Bacillus subtilis (DSM 28592, Amp-T18)

Name: Bacillus subtilis (DSM 28592, Amp-T18)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 130876

Type strain

Strain Designation Amp-T18

Culture col. no. DSM 28592 Amp-T18

NCBI tax ID(s) 1423

Special Collections DSMZ Mouse DZIF

History DSMZ <- T. Clavel, TU Munich, Freising-Weihenstephan, Germany; Amp-T18

Links

Bacillus subtilis Amp-T18 is a bacterium that was isolated from caecal content; wildtype C57BL/6 mouse treated with ampicillin.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 403373

DSM 28592

@ref 20215

Last LPSN update 2026-05-29 08:37:29

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Bacillus

Species Bacillus subtilis

Full scientific name Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

Synonyms (1)

"Vibrio subtilis"

BacDive ID	Other strains from **Bacillus subtilis** (272)	Type strain
1172	B. subtilis Marburg, 744, DSM 10, ATCC 6051, CCM 2216, ... (type strain)
962	B. subtilis DSM 1088, IFO 13169, NBRC 13169, OUT 8353
963	B. subtilis RCTI 140, DSM 1089, IFO 3026, NBRC 3026, ...
964	B. subtilis DSM 1092, IFO 3009, NBRC 3009, OUT 8235
965	B. subtilis DSM 1970, ATCC 21228, SC 8584 (SO-4)
966	B. subtilis 4-3 Ky, DSM 1971, ATCC 21394
967	B. subtilis DSM 2109, ATCC 11774, NCTC 8236, CCUG 50054, ...
969	B. subtilis SA 22, DSM 4181, NCA 72-52
970	B. subtilis natto 3335 UM4, 3335 UM4, pLS20, DSM 4449
971	B. subtilis natto 3335 UM8, pLS20, 3335 UM8, DSM 4450, ...
972	B. subtilis DSM 4451
973	B. subtilis DB163, pGR71, DSM 4515
974	B. subtilis pMW1, DSM 4608, BR 157
975	B. subtilis DSM 5552, NRS 254
976	B. subtilis DSM 5611, NRRL B-360, NRS 230
977	B. subtilis SB 491, DSM 6397, BGSC 1A2
978	B. subtilis DSM 13019, SSI MK1
979	B. subtilis DSM 21393
980	B. subtilis ES2-W11c1, DSM 30651
981	B. subtilis ES2-W11c2, DSM 30652
982	B. subtilis ES1-131, DSM 30512
983	B. subtilis ES1-179-1, DSM 30529
984	B. subtilis ES1-181-2, DSM 30533
985	B. subtilis ES1-182-1, DSM 30534
986	B. subtilis ES1-188-2, DSM 30540
987	B. subtilis ES1-188-3, DSM 30541
988	B. subtilis ES1-227, DSM 30551
989	B. subtilis ES1-257-2, DSM 30558
990	B. subtilis ES1-272, DSM 30562
991	B. subtilis ES1-306a, DSM 30570
992	B. subtilis ES1-311-1, DSM 30581
993	B. subtilis ES2-8b1, DSM 30597
994	B. subtilis FR2-W7Bb, DSM 30711
995	B. subtilis ES2-W7c1, DSM 30642
996	B. subtilis FR2-S1Ab, DSM 30671
997	B. subtilis FR2-S6Ba, DSM 30676
998	B. subtilis FR2-S7Aa, DSM 30677
999	B. subtilis FR2-S11Ca, DSM 30682
1000	B. subtilis FR1-49con, DSM 30723
1001	B. subtilis TW17/1, DSM 30801
1002	B. subtilis 1E9(pBC16), DSM 23521
1003	B. subtilis strain 168, DSM 23778
1004	B. subtilis YB886, DSM 25152
1156	B. subtilis 168, DSM 402, NBRC 111470, NCIB 10106, JCM ...
1157	B. subtilis S 237, DSM 1090, OUT 8424
1158	B. subtilis S 239, DSM 1091, OUT 8425
1159	B. subtilis DSM 3256, IAM 1213
1160	B. subtilis DSM 3257, IAM 1259, JCM 20132
1170	B. subtilis DSM 5213, BGSC 1A40, BR 151
1171	B. subtilis BD 393, DSM 5214, BGSC 1A511
1173	B. subtilis CU 1050, DSM 5545, BGSC 1A459/SU+III, HER ...
1174	B. subtilis DSM 5660, NRRL B-362, NRS 624
1175	B. subtilis 110NA, DSM 5547, HER 1243
1176	B. subtilis DSM 6198, ATCC 39090, BGSC 1S53
1177	B. subtilis RUB 331, DSM 6223, ATCC 31578
1179	B. subtilis NP40, 1A309, DSM 6887, BGSC 1A309
1180	B. subtilis 1A658, DA 65, DSM 6889, BGSC 1A658
1186	B. subtilis DSM 9565, ATCC 33677, BR 151
130881	B. subtilis T3_6, DSM 31033
130880	B. subtilis D13-1, DSM 100614
130877	B. subtilis FS3-1, DSM 100605
130878	B. subtilis D6-1, DSM 100612
130879	B. subtilis D8-1, DSM 100613
22915	B. subtilis P5/4_1, DSM 30924
22916	B. subtilis P8/5_1, DSM 30927
22917	B. subtilis P8/15_1, DSM 30928
22918	B. subtilis P10/7_1, DSM 30929
22919	B. subtilis P10/14_1, DSM 30925
23667	B. subtilis D1, DSM 30941
23668	B. subtilis D-FC1, DSM 30942
130185	B. subtilis G20, DSM 31020
130186	B. subtilis G23, DSM 31021
130187	B. subtilis b9_4, DSM 31008
130188	B. subtilis b9_5, DSM 31009
130189	B. subtilis b9_6, DSM 31010
131652	B. subtilis Stev2, DSM 103044
131653	B. subtilis Stev9, DSM 103047
131654	B. subtilis Stev18, DSM 103051
131655	B. subtilis D36_1, DSM 103758
134612	B. subtilis PM 15, CIP 76.53
134701	B. subtilis AA 56, CIP 76.87
134853	B. subtilis FS 437, CIP 76.34
134854	B. subtilis PM 43, CIP 76.63
134856	B. subtilis FS 32, CIP 76.81
134857	B. subtilis AA 77, CIP 76.91
134858	B. subtilis AA 79, CIP 76.92
134859	B. subtilis AA 92, CIP 76.93
135155	B. subtilis FA 10, CIP 76.72
135173	B. subtilis FF 25, CIP 76.80
135255	B. subtilis CIP 51.55
135269	B. subtilis FD 10, CIP 76.29
135270	B. subtilis FE 9, CIP 76.30
135280	B. subtilis CIP 61.6, ATCC 4529, NRRL NRS-1004, NRRL: ...
135297	B. subtilis PM 16, CIP 76.54
135365	B. subtilis NRS-230, CIP 52.66
135516	B. subtilis 1576, CIP 51.15
135545	B. subtilis CIP 51.51
135547	B. subtilis PM 14, CIP 76.52
135577	B. subtilis PM 44, CIP 76.64
135581	B. subtilis PM 26, CIP 76.56
135590	B. subtilis PM 32, CIP 76.57
135620	B. subtilis 5a, CIP 61.7, NCTC 2590
135643	B. subtilis PM 36, CIP 76.58
135676	B. subtilis PM 39, CIP 76.60
135695	B. subtilis AB 93-8-1, CIP 76.44
135696	B. subtilis AB 53, CIP 76.103
135697	B. subtilis AB 72, CIP 76.104
135698	B. subtilis AB 73, CIP 76.105
135699	B. subtilis AD 8, CIP 76.106
135700	B. subtilis AD 19, CIP 76.107
135701	B. subtilis AA 48, CIP 76.108
135783	B. subtilis PM 42, CIP 76.62
135784	B. subtilis EV 15, CIP 76.70
135900	B. subtilis CIP 104473, ATCC 19659, IFO 13722, PRD 66
135909	B. subtilis 3R9675, CIP 54.10, ATCC 9524
136058	B. subtilis CIP A33
136375	B. subtilis B-558, CIP 53.159, ATCC 9466, IAM 1026, NCDO ...
136391	B. subtilis CIP 53.173, NCTC 2121
136392	B. subtilis CIP 53.174, ATCC 8190, NCTC 2118
136394	B. subtilis CIP 53.176, NCTC 2116
136395	B. subtilis CIP 53.178, NCTC 2123
136501	B. subtilis 168 CA, CIP 106309
136948	B. subtilis AA 4, CIP 76.83
136959	B. subtilis AA 16, CIP 76.85
136969	B. subtilis AA 5, CIP 76.84
136972	B. subtilis AA 51, CIP 76.86
136975	B. subtilis AA 57, CIP 76.88
136991	B. subtilis AA 62, CIP 76.89
136992	B. subtilis AA 64, CIP 76.90
137036	B. subtilis B2, CIP 55.96
137087	B. subtilis FE 6, CIP 76.75
137100	B. subtilis PM 25, CIP 76.55
137129	B. subtilis AA 2, CIP 76.82
137272	B. subtilis 796, CIP 104611, ATCC 82, ATCC 8037
137292	B. subtilis CIP 53.179, ATCC 8188, NCTC 2122
137348	B. subtilis FS 381, CIP 76.32
137349	B. subtilis FS 436, CIP 76.33
137416	B. subtilis PM 13, CIP 76.51
137419	B. subtilis AC 27, CIP 76.96
137433	B. subtilis AC 28, CIP 76.97
137537	B. subtilis CIP A4
137923	B. subtilis 99-86, CIP 102199
138447	B. subtilis CIP 51.48
138480	B. subtilis CIP 51.54
138711	B. subtilis PM 37, CIP 76.48
138730	B. subtilis PM 40, CIP 76.49
138739	B. subtilis PM 7, CIP 76.50
138763	B. subtilis AC 32, CIP 76.99
138773	B. subtilis AC 33, CIP 76.100
138777	B. subtilis AC 34, CIP 76.101
138779	B. subtilis AC 35, CIP 76.102
138875	B. subtilis FE 19, CIP 76.79
138909	B. subtilis Caron, CIP A155
138915	B. subtilis PM 38, CIP 76.59
138956	B. subtilis AB 12, CIP 76.35
138957	B. subtilis 168, CIP 76.45
138958	B. subtilis PM 6, CIP 76.46
138959	B. subtilis CB 22, CIP 76.26
138964	B. subtilis DB 16, CIP 76.27
138965	B. subtilis FA 10, CIP 76.28
138967	B. subtilis FS 336, CIP 76.31
138968	B. subtilis FS 223, CIP 76.38
138970	B. subtilis FS 319, CIP 76.36
138971	B. subtilis AB 35, CIP 76.37
138972	B. subtilis FS 382, CIP 76.39
138973	B. subtilis AB 34, CIP 76.40
138974	B. subtilis AA 32, CIP 76.41
138975	B. subtilis AB 91-2-2, CIP 76.43
138976	B. subtilis PM 9, CIP 76.47
138979	B. subtilis PM 41, CIP 76.61
138980	B. subtilis PM 47, CIP 76.65
138981	B. subtilis PM 49, CIP 76.66
138982	B. subtilis SB 5-2, CIP 76.67
138983	B. subtilis BD 68, CIP 76.68
138984	B. subtilis EC 21, CIP 76.69
138985	B. subtilis EV 16, CIP 76.71
138986	B. subtilis FA 20-1, CIP 76.73
138987	B. subtilis FC 10, CIP 76.74
138988	B. subtilis FE 10, CIP 76.76
138989	B. subtilis FE 20, CIP 76.77
138990	B. subtilis FF 15, CIP 76.78
138992	B. subtilis AA 94, CIP 76.94
138993	B. subtilis AB 8, CIP 76.95
138994	B. subtilis AC 31, CIP 76.98
138995	B. subtilis AB 34-1, CIP 76.42
139174	B. subtilis 543, CIP 51.49
139209	B. subtilis CIP 102517
139407	B. subtilis CIP 53.175, NCTC 2119
139411	B. subtilis CIP 53.177, NCTC 2117
139520	B. subtilis Parry1, CIP 110271, NCIMB 13061, NCTC 5398, ...
139538	B. subtilis RS2008, CIP 109935
139539	B. subtilis CIP 63.8
141283	B. subtilis CCUG 625
141686	B. subtilis CCUG 4783
142101	B. subtilis CCUG 10780, ATCC 12432, NCTC 10315, ATCC ...
142397	B. subtilis CCUG 12316
145153	B. subtilis CCUG 27765, NRRL NRS-356, NRRL NRS-238, NRRL ...
146436	B. subtilis CCUG 31131
146685	B. subtilis CCUG 31949
146735	B. subtilis CCUG 32040
147533	B. subtilis CCUG 33755
147568	B. subtilis CCUG 33816
148233	B. subtilis CCUG 35081
148234	B. subtilis CCUG 35082
148235	B. subtilis CCUG 35083
148236	B. subtilis CCUG 35084
148237	B. subtilis CCUG 35085
148238	B. subtilis CCUG 35086
148239	B. subtilis CCUG 35087
148240	B. subtilis CCUG 35088
148241	B. subtilis CCUG 35089
148242	B. subtilis CCUG 35090
148243	B. subtilis CCUG 35091
148244	B. subtilis CCUG 35092
148245	B. subtilis CCUG 35093
148436	B. subtilis CCUG 35445
149160	B. subtilis CCUG 36967
149203	B. subtilis CCUG 37263
149428	B. subtilis CCUG 37730
149469	B. subtilis CCUG 37799
149478	B. subtilis CCUG 37815 A
149479	B. subtilis CCUG 37815 B
149600	B. subtilis CCUG 38041
149872	B. subtilis CCUG 38587
150261	B. subtilis CCUG 39418
150473	B. subtilis CCUG 41392
150474	B. subtilis CCUG 41393
151897	B. subtilis CCUG 45145
153228	B. subtilis CCUG 48815 A
157307	B. subtilis IMI 347329
157622	B. subtilis 2dA_C27, DSM 103912
157623	B. subtilis SB-14, DSM 109343
158869	B. subtilis Fh040319_C2, DSM 109511
158870	B. subtilis Fh040319_C10, DSM 109512
158871	B. subtilis Fh040319_C12, DSM 109513
158872	B. subtilis Fh040319_C14, DSM 109514
158873	B. subtilis FH4_1, DSM 110116
161119	B. subtilis JCM 11003
162225	B. subtilis JCM 18293
162706	B. subtilis JCM 20036, IAM 1071
162708	B. subtilis JCM 20038, IAM 1076
162718	B. subtilis JCM 20057, IAM 1114
162729	B. subtilis JCM 20073, ATCC 14593, IAM 1145
162736	B. subtilis JCM 20083, IAM 1163
162738	B. subtilis JCM 20085, IAM 1168
162739	B. subtilis JCM 20086, IAM 1169
162746	B. subtilis JCM 20094, IAM 1191
162747	B. subtilis JCM 20095, IAM 1192
162748	B. subtilis JCM 20096, IAM 1193, IFO 3033, NBRC 3033
162755	B. subtilis JCM 20105, IAM 1207
162758	B. subtilis JCM 20108, IAM 1212
162767	B. subtilis JCM 20118, IAM 1231
162773	B. subtilis JCM 20127, IAM 1247
162882	B. subtilis JCM 20333, ATCC 4925, IAM 11060
162883	B. subtilis JCM 20336, ATCC 6460, IAM 11063
162890	B. subtilis JCM 20352, IAM 12019
162891	B. subtilis JCM 20353, IAM 12020
162952	B. subtilis JCM 20520, IAM 12366
163112	B. subtilis JCM 21228, IAM 14446
163798	B. subtilis JCM 30446
164104	B. subtilis JCM 31626
164106	B. subtilis JCM 31628
164275	B. subtilis JCM 32485
174177	B. subtilis CIMB 05-2416, CRBIP17.22
174358	B. subtilis P7, DSM 115556
174359	B. subtilis ZH1, DSM 115557
176404	B. subtilis SZB3, DSM 115815
176405	B. subtilis BEST7003, Bacillus subtilis BEST7003, DSM ...
176406	B. subtilis BP20.15, DSM 119457
176407	B. subtilis BB10.1, DSM 119458
177878	B. subtilis FSU-2739, SF002739
177901	B. subtilis b 1, SF002793

Morphology

Colony morphology

22185

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
22185	LB (Luria-Bertani) MEDIUM (DSMZ Medium 381)		Medium recipe at MediaDive	Name: LB (Luria-Bertani) MEDIUM (DSMZ Medium 381) Composition: Agar 20.0 g/l NaCl 10.0 g/l Tryptone 10.0 g/l Yeast extract 5.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
22185	positive	growth	37

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125438		95.248

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Patient	#Antibiotic treatment
#Host	#Mammals	#Muridae (Mouse/Rat)
#Host Body-Site	#Gastrointestinal tract	#Large intestine
#Host Body Product	#Gastrointestinal tract	#Caecal content

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
22185	caecal content; wildtype C57BL/6 mouse treated with ampicillin	Freising	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence KR364738 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
22185	yes, in single cases	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM2462308v1 assembly for Bacillus subtilis DSM 28592	contig	GCA_024623085	1423.2387	1423	72.17

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
22185	Bacillus sp. Amp-T18 16S ribosomal RNA gene, partial sequence	KR364738	1451		1796611

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2462308v1 assembly for Bacillus subtilis DSM 28592
NCBI: GCA_024623085

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	87.60	no
125439	motility	BacteriaNetⓘ	yes	55.39	no
125439	gram_stain	BacteriaNetⓘ	variable	67.56	no
125439	spore_formation	BacteriaNetⓘ	yes	56.02	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bacillus subtilis DSM 28592
NCBI: GCA_024623085.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	81.39	no
125438	anaerobic	anaerobicⓘ	no	88.33	no
125438	aerobic	aerobicⓘ	yes	69.63	no
125438	spore-forming	spore-formingⓘ	yes	95.25	no
125438	thermophilic	thermophileⓘ	no	95.23	yes
125438	flagellated	motile2+ⓘ	yes	88.28	no

Literature

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#22185	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28592
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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