Name: Bacillus subtilis 168
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1156

Type strain:

Species: Bacillus subtilis

Strain Designation: 168

Culture col. no.: DSM 402, NBRC 111470, NCIB 10106, JCM 10629, ATCC 33234, KCTC 2217, NCIMB 10106

Strain history: H. Yoshikawa <-- S. Dusko Ehrich <-- C. Anagnostopoulos <-- J. Spizizen 168 (trpC2).

NCBI tax ID(s): 1423 (species)

SI-ID 38268

ATCC 23857, ATCC 33234, NCIB 10106, DSM 402, NCIMB 10106, NCIMB 10263, KCTC 1326, KCTC 2217, JCM 10629, BCRC 10614, CCRC 10614, VTT E-04026, LMG 19457

Special Collections

Bacillus phage vB_BsuP-Goe1 (Bacillus virus Goe1) Bacillus phage vB_BsuM-Goe9

Other strains from Bacillus subtilis

Section

Bacillus subtilis 168 is a spore-forming, Gram-positive human pathogen of the family Bacillaceae.

Gram-positive
spore-forming
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus subtilis**
	Full Scientific Name (LPSN)	Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

Synonym

"Vibrio subtilis"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #182]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #182]

Culture medium growth

[Ref.: #182]

Culture medium link

Medium recipe at MediaDive

[Ref.: #182]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #182]			growth	30 °C
[Ref.: #67770]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Ability of spore formation

yes Confidence in %95.341

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence AB598744 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #182]	Pathogenicity (human)	yes, in single cases
[Ref.: #182]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bacillus subtilis subsp. subtilis gene for 16S rRNA, partial sequence, strain: JCM 10629	AB598744	1472	GenBank ENA	135461 tax ID
[Ref.: #124043]	Bacillus subtilis strain KCTC 2217 16S ribosomal RNA gene, partial sequence.	FJ969740	1454	GenBank ENA	1423 tax ID	*
[Ref.: #124043]	Bacillus subtilis strain KCTC 2217 16S-23S ribosomal RNA intergenic spacer, complete sequence.	FJ969780	165	GenBank ENA	1423 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Bacillus subtilis subsp. subtilis str. 168	GCA_000696635	scaffold	GenBank ENA	224308 tax ID
[Ref.: #66792]	Bacillus subtilis subsp. subtilis str. 168	224308.482	complete	PATRIC	224308 tax ID	*
[Ref.: #66792]	Bacillus subtilis subsp. subtilis str. 168	GCA_012931705	complete	GenBank ENA	224308 tax ID	*
[Ref.: #66792]	Bacillus subtilis subsp. subtilis str. 168	GCA_000789275	complete	GenBank ENA	224308 tax ID	*
[Ref.: #66792]	Bacillus subtilis subsp. subtilis str. 168	GCA_002009135	chromosome	GenBank ENA	224308 tax ID	*
[Ref.: #66792]	Bacillus subtilis subsp. subtilis str. 168	GCA_002009095	chromosome	GenBank ENA	224308 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus subtilis subsp. subtilis str. 168 168 NCBI: GCA_000009045.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	83.726	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	89.462	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	70.701	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	95.341	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.091	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	88.402	no

Availability in culture collections External links

[Ref.: #182]

Culture collection no.

DSM 402, NBRC 111470, NCIB 10106, JCM 10629, ATCC 33234, KCTC 2217, NCIMB 10106

[Ref.: #70827]

SI-ID 38268


[Ref.: #124042]	Bacillus phage vB_BsuP-Goe1 (Bacillus virus Goe1)
[Ref.: #124042]	Bacillus phage vB_BsuM-Goe9

Literature:

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Boosting isoprene production via heterologous expression of the Kudzu isoprene synthase gene (kIspS) into Bacillus spp. cell factory.	Gomaa L, Loscar ME, Zein HS, Abdel-Ghaffar N, Abdelhadi AA, Abdelaal AS, Abdallah NA	AMB Express	10.1186/s13568-017-0461-7	2017	*
Enzymology	Deposition of Bacillus subtilis spores using an airbrush-spray or spots to study surface decontamination by pulsed light.	Levy C, Bornard I, Carlin F	J Microbiol Methods	10.1016/j.mimet.2010.11.021	2010	*
Enzymology	A versatile esterase from Bacillus subtilis: cloning, expression, characterization, and its application in biocatalysis.	Schmidt M, Henke E, Heinze B, Kourist R, Hidalgo A, Bornscheuer UT	Biotechnol J	10.1002/biot.200600174	2007	*
Enzymology	Cloning and expression of the lysostaphin gene in Bacillus subtilis and Lactobacillus casei.	Gaier W, Vogel RF, Hammes WP	Lett Appl Microbiol	10.1111/j.1472-765x.1992.tb00651.x	1992	*

References

#182	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 402

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#70827

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38268.1 )

#124042

Dr. Johannes Wittmann, Dr. Clara Rolland, Dr. Lorenz Reimer, Joaquim Sardà: PhageDive .

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

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