Name: Bacillus subtilis natto 3335 UM4
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 970

Type strain:

Species: Bacillus subtilis

Strain Designation: natto 3335 UM4, 3335 UM4, pLS20

Culture col. no.: DSM 4449

NCBI tax ID(s): 1423 (species)

SI-ID 112709

DSM 4449

Special Collections

DSMZ

Other strains from Bacillus subtilis

Bacillus subtilis natto 3335 UM4 is a human pathogen of the family Bacillaceae.

human pathogen
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus subtilis**
	Full Scientific Name (LPSN)	Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

Synonym

"Vibrio subtilis"

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1667]	Pathogenicity (human)	yes
[Ref.: #1667]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Availability in culture collections External links

[Ref.: #1667]

Culture collection no.

DSM 4449

[Ref.: #70643]

SI-ID 112709

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Metabolism	A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation.	Mori K, Fukui K, Amatsu R, Ishikawa S, Verrone V, Wipat A, Meijer WJJ, Yoshida KI	Microb Cell Fact	10.1186/s12934-022-01759-8	2022	*
Genetics	A Conserved Class II Type Thioester Domain-Containing Adhesin Is Required for Efficient Conjugation in Bacillus subtilis.	Gago-Cordoba C, Val-Calvo J, Abia D, Diaz-Talavera A, Miguel-Arribas A, Aguilar Suarez R, van Dijl JM, Wu LJ, Meijer WJJ	mBio	10.1128/mBio.00104-21	2021	*
Metabolism	Reversible regulation of conjugation of Bacillus subtilis plasmid pLS20 by the quorum sensing peptide responsive anti-repressor RappLS20.	Singh PK, Serrano E, Ramachandran G, Miguel-Arribas A, Gago-Cordoba C, Val-Calvo J, Lopez-Perez A, Alfonso C, Wu LJ, Luque-Ortega JR, Meijer WJJ	Nucleic Acids Res	10.1093/nar/gkaa797	2020	*
Metabolism	Inactivation of the dimeric RappLS20 anti-repressor of the conjugation operon is mediated by peptide-induced tetramerization.	Crespo I, Bernardo N, Miguel-Arribas A, Singh PK, Luque-Ortega JR, Alfonso C, Malfois M, Meijer WJJ, Boer DR	Nucleic Acids Res	10.1093/nar/gkaa540	2020	*
Biotechnology	Stable mutants of restriction-deficient/modification-proficient Bacillus subtilis 168: hub strains for giant DNA engineering.	Itaya M, Sato M, Watanabe S, Yoshikawa H, Tomita M, Sato R	J Biochem	10.1093/jb/mvz031	2019	*
Genetics	The first high frequency of recombination-like conjugal transfer from an integrated origin of transfer sequence in Bacillus subtilis 168.	Itaya M, Hasegawa M, Tomita M, Sato M	Biosci Biotechnol Biochem	10.1080/09168451.2017.1422970	2018	*
Enzymology	Discovery of a new family of relaxases in Firmicutes bacteria.	Ramachandran G, Miguel-Arribas A, Abia D, Singh PK, Crespo I, Gago-Cordoba C, Hao JA, Luque-Ortega JR, Alfonso C, Wu LJ, Boer DR, Meijer WJ	PLoS Genet	10.1371/journal.pgen.1006586	2017	*
Pathogenicity	The presence of conjugative plasmid pLS20 affects global transcription of Its Bacillus subtilis host and confers beneficial stress resistance to cells.	Rosch TC, Golman W, Hucklesby L, Gonzalez-Pastor JE, Graumann PL	Appl Environ Microbiol	10.1128/AEM.03154-13	2013	*
Genetics	Mobility of the native Bacillus subtilis conjugative plasmid pLS20 is regulated by intercellular signaling.	Singh PK, Ramachandran G, Ramos-Ruiz R, Peiro-Pastor R, Abia D, Wu LJ, Meijer WJ	PLoS Genet	10.1371/journal.pgen.1003892	2013	*
Metabolism	Inhibition of Bacillus subtilis natural competence by a native, conjugative plasmid-encoded comK repressor protein.	Singh PK, Ramachandran G, Duran-Alcalde L, Alonso C, Wu LJ, Meijer WJ	Environ Microbiol	10.1111/j.1462-2920.2012.02819.x	2012	*
Enzymology	Serial assembly of Thermus megaplasmid DNA in the genome of Bacillus subtilis 168: a BAC-based domino method applied to DNA with a high GC content.	Ohtani N, Hasegawa M, Sato M, Tomita M, Kaneko S, Itaya M	Biotechnol J	10.1002/biot.201100396	2012	*
Metabolism	Alp7R regulates expression of the actin-like protein Alp7A in Bacillus subtilis.	Derman AI, Nonejuie P, Michel BC, Truong BD, Fujioka A, Erb ML, Pogliano J	J Bacteriol	10.1128/JB.06550-11	2012	*
Metabolism	Localization pattern of conjugation machinery in a Gram-positive bacterium.	Bauer T, Rosch T, Itaya M, Graumann PL	J Bacteriol	10.1128/JB.00175-11	2011	*
Genetics	Complete nucleotide sequence and determination of the replication region of the sporulation inhibiting plasmid p576 from Bacillus pumilus NRS576.	Singh PK, Ballestero-Beltran S, Ramachandran G, Meijer WJ	Res Microbiol	10.1016/j.resmic.2010.07.007	2010	*
Enzymology	Conjugational transfer system to shuttle giant DNA cloned by Bacillus subtilis genome (BGM) vector.	Kuroki A, Ohtani N, Tsuge K, Tomita M, Itaya M	Gene	10.1016/j.gene.2007.04.030	2007	*
Enzymology	Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium.	Itaya M, Sakaya N, Matsunaga S, Fujita K, Kaneko S	Biosci Biotechnol Biochem	10.1271/bbb.70.740	2006	*
Metabolism	Identification and characterization of a novel type of replication terminator with bidirectional activity on the Bacillus subtilis theta plasmid pLS20.	Meijer WJ, Smith M, Wake RG, de Boer AL, Venema G, Bron S	Mol Microbiol	10.1111/j.1365-2958.1996.tb02474.x	1996	*
Genetics	Characterization of the replication region of the Bacillus subtilis plasmid pLS20: a novel type of replicon.	Meijer WJ, de Boer AJ, van Tongeren S, Venema G, Bron S	Nucleic Acids Res	10.1093/nar/23.16.3214	1995	*
Genetics	Bacillus subtilis (natto) plasmid pLS20 mediates interspecies plasmid transfer.	Koehler TM, Thorne CB	J Bacteriol	10.1128/jb.169.11.5271-5278.1987	1987	*
	Bacillus subtilis 168 as a unique platform enabling synthesis and dissemination of genomes.	Itaya M	J Gen Appl Microbiol	10.2323/jgam.2021.12.001	2022	*
	Structural and biochemical characterization of the relaxosome auxiliary proteins encoded on the Bacillus subtilis plasmid pLS20.	Crespo I, Bernardo N, Cuppari A, Calisto BM, Val-Calvo J, Miguel-Arribas A, Meijer WJJ, Carpena X, Gil-Ortiz F, Malfois M, Boer DR	Comput Struct Biotechnol J	10.1016/j.csbj.2021.12.041	2022	*
	Establishment Genes Present on pLS20 Family of Conjugative Plasmids Are Regulated in Two Different Ways.	Val-Calvo J, Miguel-Arribas A, Freire F, Abia D, Wu LJ, Meijer WJJ	Microorganisms	10.3390/microorganisms9122465	2021	*
	pLS20 is the archetype of a new family of conjugative plasmids harboured by Bacillus species.	Val-Calvo J, Miguel-Arribas A, Abia D, Wu LJ, Meijer WJJ	NAR Genom Bioinform	10.1093/nargab/lqab096	2021	*
	Assessment of Bacillus subtilis Plasmid pLS20 Conjugation in the Absence of Quorum Sensing Repression.	Mori K, Verrone V, Amatsu R, Fukui K, Meijer WJJ, Ishikawa S, Wipat A, Yoshida KI	Microorganisms	10.3390/microorganisms9091931	2021	*
	Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20.	Meijer WJJ, Boer DR, Ares S, Alfonso C, Rojo F, Luque-Ortega JR, Wu LJ	Front Mol Biosci	10.3389/fmolb.2021.648468	2021	*
	Surface Exclusion Revisited: Function Related to Differential Expression of the Surface Exclusion System of Bacillus subtilis Plasmid pLS20.	Gago-Cordoba C, Val-Calvo J, Miguel-Arribas A, Serrano E, Singh PK, Abia D, Wu LJ, Meijer WJJ	Front Microbiol	10.3389/fmicb.2019.01502	2019	*
	Stable and efficient delivery of DNA to Bacillus subtilis (natto) using pLS20 conjugational transfer plasmids.	Itaya M, Nagasaku M, Shimada T, Ohtani N, Shiwa Y, Yoshikawa H, Kaneko S, Tomita M, Sato M	FEMS Microbiol Lett	10.1093/femsle/fnz032	2019	*
	Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer.	Miyano M, Tanaka K, Ishikawa S, Takenaka S, Miguel-Arribas A, Meijer WJJ, Yoshida KI	Microb Cell Fact	10.1186/s12934-017-0855-x	2018	*
	The Bacillus subtilis Conjugative Plasmid pLS20 Encodes Two Ribbon-Helix-Helix Type Auxiliary Relaxosome Proteins That Are Essential for Conjugation.	Miguel-Arribas A, Hao JA, Luque-Ortega JR, Ramachandran G, Val-Calvo J, Gago-Cordoba C, Gonzalez-Alvarez D, Abia D, Alfonso C, Wu LJ, Meijer WJJ	Front Microbiol	10.3389/fmicb.2017.02138	2017	*
	Induction of Plasmid Conjugation in Bacillus subtilis Is Bistable and Driven by a Direct Interaction of a Rap/Phr Quorum-sensing System with a Master Repressor.	Rosch TC, Graumann PL	J Biol Chem	10.1074/jbc.M115.664110	2015	*
	A complex genetic switch involving overlapping divergent promoters and DNA looping regulates expression of conjugation genes of a gram-positive plasmid.	Ramachandran G, Singh PK, Luque-Ortega JR, Yuste L, Alfonso C, Rojo F, Wu LJ, Meijer WJ	PLoS Genet	10.1371/journal.pgen.1004733	2014	*
	Diverse regulatory circuits for transfer of conjugative elements.	Singh PK, Meijer WJ	FEMS Microbiol Lett	10.1111/1574-6968.12526	2014	*
	Restriction on conjugational transfer of pLS20 in Bacillus subtilis 168.	Ohtani N, Sato M, Tomita M, Itaya M	Biosci Biotechnol Biochem	10.1271/bbb.80315	2008	*
	Experimental basis for a stable plasmid, pLS30, to shuttle between Bacillus subtilis species by conjugational transfer.	Sakaya N, Kaneko S, Matsunaga S, Itaya M	J Biochem	10.1093/jb/mvj058	2006	*
	Mobilization of "nonmobilizable" plasmids by the aggregation-mediated conjugation system of Bacillus thuringiensis.	Andrup L, Jorgensen O, Wilcks A, Smidt L, Jensen GB	Plasmid	10.1006/plas.1996.0035	1996	*
	A New Tool for the Flexible Genetic Manipulation of Geobacillus kaustophilus.	Amatsu R, Mori K, Ishikawa S, Meijer WJJ, Yoshida KI	Bio Protoc	10.21769/BioProtoc.4502	2022	*
	Effective plasmid delivery to a plasmid-free Bacillus natto strain by a conjugational transfer system.	Itaya M, Sato M, Watanabe S, Kataoka M	J Biochem	10.1093/jb/mvac069	2022	*

References

#1667

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4449

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#70643

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID112709.1 )

* These data were automatically processed and therefore are not curated

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