Name: Bacillus subtilis CIP 104473
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 135900

Type strain:

Species: Bacillus subtilis

Culture col. no.: CIP 104473, ATCC 19659, IFO 13722, PRD 66

Strain history: CIP <- 1995, ATCC <- C.H. Shaffer, Jr.: strain PRD66

NCBI tax ID(s): 1423 (species)

SI-ID 7465

LMG 13579, ATCC 19659, IFO 13722, NCIMB 12900, CIP 104473, NBIMCC 3562, NCIMB 13818, NBRC 13722, CCT 0926, CDBB 1009, NRRL B-14471, NRRL B-41757

Other strains from Bacillus subtilis

Section

Bacillus subtilis CIP 104473 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from Unknown source.

Gram-positive
motile
rod-shaped
facultative anaerobe
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus subtilis**
	Full Scientific Name (LPSN)	Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

Synonym

"Vibrio subtilis"

Information on morphological and physiological properties Morphology

[Ref.: #35164]	Gram stain	positive

[Ref.: #35164]	Cell shape	rod-shaped

[Ref.: #35164]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #35164]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #35164]

Culture medium growth

[Ref.: #35164]

Culture medium composition

expand / minimize

[Ref.: #35164]

Culture medium

CIP Medium 3

[Ref.: #35164]

Culture medium growth

[Ref.: #35164]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #35164]

Culture medium

CIP Medium 6

[Ref.: #35164]

Culture medium growth

[Ref.: #35164]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #35164]

Culture medium

CIP Medium 72

[Ref.: #35164]

Culture medium growth

[Ref.: #35164]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #35164]			growth	30 °C

[Ref.: #35164]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #35164]

Oxygen tolerance

facultative anaerobe

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #35164]		nitrate ChEBI	+	reduction
[Ref.: #35164]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #35164]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #35164]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #35164]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #35164]	urease	+	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #35164]	7981	-	+	+	+	-	+	-	-	-	-	-	-	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #35164]	Sample type/isolated from	Unknown source
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #35164]

Biosafety level

Risk group (French classification)

Availability in culture collections External links

[Ref.: #35164]

Culture collection no.

CIP 104473, ATCC 19659, IFO 13722, PRD 66

[Ref.: #93171]

SI-ID 7465

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Enzymology	Anatomical and Phytochemical Characteristics of Different Parts of Hypericum scabrum L. Extracts, Essential Oils, and Their Antimicrobial Potential.	Ergin KN, Karakaya S, Goger G, Sytar O, Demirci B, Duman H	Molecules	10.3390/molecules27041228	2022	*
Pathogenicity	Recent Patents on Impact of Lipopeptide on the Biofilm Formation onto Titanium and Stainless Steel Surfaces.	Pires MEE, Parreira AG, Silva TNL, Colares HC, da Silva JA, de Magalhaes JT, Galdino AS, Goncalves DB, Granjeiro JM, Granjeiro PA	Recent Pat Biotechnol	10.2174/1872208313666190822150323	2020	*
Pathogenicity	The differential effects of heat-shocking on the viability of spores from Bacillus anthracis, Bacillus subtilis, and Clostridium sporogenes after treatment with peracetic acid- and glutaraldehyde-based disinfectants.	March JK, Pratt MD, Lowe CW, Cohen MN, Satterfield BA, Schaalje B, O'Neill KL, Robison RA	Microbiologyopen	10.1002/mbo3.277	2015	*
Pathogenicity	Antimicrobial activity of Carpolobia lutea extracts and fractions.	Nwidu LL, Nwafor PA, Vilegas W	Afr J Tradit Complement Altern Med		2012	*
Metabolism	Real-time PCR investigation on the expression of sboA and ituD genes in Bacillus spp.	Velho RV, Caldas DG, Medina LF, Tsai SM, Brandelli A	Lett Appl Microbiol	10.1111/j.1472-765X.2011.03060.x	2011	*
Pathogenicity	Effects of minerals on resistance of Bacillus subtilis spores to heat and hydrostatic pressure.	Igura N, Kamimura Y, Islam MS, Shimoda M, Hayakawa I	Appl Environ Microbiol	10.1128/AEM.69.10.6307-6310.2003	2003	*
Pathogenicity	Disinfection of Bacillus subtilis spores with chlorine dioxide: a bench-scale and pilot-scale study.	Radziminski C, Ballantyne L, Hodson J, Creason R, Andrews RC, Chauret C	Water Res	10.1016/s0043-1354(01)00355-4	2002	*
Biotechnology	Growth of nisin-producing lactococci in cooked rice supplemented with soybean extract and its application to inhibition of Bacillus subtilis in rice miso.	Kato T, Inuzuka L, Kondo M, Matsuda T	Biosci Biotechnol Biochem	10.1271/bbb.65.330	2001	*
Pathogenicity	Evaluation of microbial loads of Bacillus subtilis spores on penicylinders.	Danielson JW	J AOAC Int		1993	*
Enzymology	Effect of Dietary Supplementation of Bacillus subtilis on Growth Performance, Organ Weight, Digestive Enzyme Activities, and Serum Biochemical Indices in Broiler.	Mohamed TM, Sun W, Bumbie GZ, Dosoky WM, Rao Z, Hu P, Wu L, Tang Z	Animals (Basel)	10.3390/ani12121558	2022	*
	Feeding Bacillus subtilis ATCC19659 to Broiler Chickens Enhances Growth Performance and Immune Function by Modulating Intestinal Morphology and Cecum Microbiota.	Mohamed TM, Sun W, Bumbie GZ, Elokil AA, Mohammed KAF, Zebin R, Hu P, Wu L, Tang Z	Front Microbiol	10.3389/fmicb.2021.798350	2022	*
	Comparison of the sporicidal activity of a UV disinfection process with three FDA cleared sterilants.	Pineau L, Radix C, Weber DJ	Am J Infect Control	10.1016/j.ajic.2022.02.027	2022	*
	Synthesis, Antibacterial, and Cytotoxicity Evaluation of Oleanolic Acid-4-aminoquinoline Based Hybrid Compounds.	Khwaza V, Oyedeji OO, Aderibigbe BA, Morifi E, Fonkui TY, Ndinteh DT, Steenkamp V	Recent Adv Antiinfect Drug Discov	10.2174/1574891X16666210210165547	2021	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#35164 Collection of Institut Pasteur ; Curators of the CIP; CIP 104473

#68382

Automatically annotated from API zym .

#93171

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7465.1 )

* These data were automatically processed and therefore are not curated

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