Strain identifier

BacDive ID: 137923

Type strain: No

Species: Bacillus subtilis

Strain Designation: 99-86

Strain history: CIP <- 1986, Lab. Ident. Inst. Pasteur, Paris, France: strain 99-86 <- Saint Joseph Hosp., Paris, France

NCBI tax ID(s): 1423 (species)

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General

@ref: 38872

BacDive-ID: 137923

keywords: Bacteria, facultative anaerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Bacillus subtilis 99-86 is a facultative anaerobe, spore-forming, Gram-positive bacterium of the family Bacillaceae.

NCBI tax id

  • NCBI tax id: 1423
  • Matching level: species

strain history

  • @ref: 38872
  • history: CIP <- 1986, Lab. Ident. Inst. Pasteur, Paris, France: strain 99-86 <- Saint Joseph Hosp., Paris, France

doi: 10.13145/bacdive137923.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus subtilis
  • full scientific name: Bacillus subtilis (Ehrenberg 1835) Cohn 1872 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Vibrio subtilis

@ref: 38872

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Bacillus

species: Bacillus subtilis

strain designation: 99-86

type strain: no

Morphology

cell morphology

  • @ref: 38872
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 38872
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
38872MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
38872CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
38872CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
38872positivegrowth30
38872positivegrowth22-37
38872nogrowth10
38872nogrowth45
38872nogrowth55

culture pH

  • @ref: 38872
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 38872
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 38872
  • spore formation: yes

halophily

  • @ref: 38872
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3887216947citrate+carbon source
388724853esculin+hydrolysis
38872606565hippurate-hydrolysis
3887217632nitrate+reduction
3887216301nitrite-reduction
3887217632nitrate+respiration

metabolite production

  • @ref: 38872
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
3887215688acetoin+
3887217234glucose-

enzymes

@refvalueactivityec
38872oxidase+
38872beta-galactosidase+3.2.1.23
38872alcohol dehydrogenase-1.1.1.1
38872gelatinase+
38872amylase+
38872DNase+
38872caseinase+3.4.21.50
38872catalase+1.11.1.6
38872tween esterase+
38872gamma-glutamyltransferase+2.3.2.2
38872lecithinase-
38872lipase-
38872lysine decarboxylase-4.1.1.18
38872ornithine decarboxylase-4.1.1.17
38872protease+
38872urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase-3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
38872-+++------+-++-++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
38872+++++-+++++-+++++++++++--------++++++----+-++----+++-+-+---++------------+-+---++-----+--++++++----

Isolation, sampling and environmental information

isolation

@refgeographic locationcountryorigin.countrycontinentsample typeisolation date
38872ParisFranceFRAEurope
38872ParisFranceFRAEuropeHuman, Sinus1986

Safety information

risk assessment

  • @ref: 38872
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

External links

@ref: 38872

culture collection no.: CIP 102199

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
38872Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20102199Collection of Institut Pasteur (CIP 102199)
68382Automatically annotated from API zym