Name: Clostridium perfringens CIP 104880
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 138119

Type strain:

Species: Clostridium perfringens

Culture col. no.: CIP 104880, ATCC 12917, NCTC 8239, CCUG 18370

Strain history: CIP <- 1996, NCTC <- B.C. Hobbs, PHLS, London, UK

NCBI tax ID(s): 451757 (strain), 1502 (species)

SI-ID 35683

ATCC 12917, NCTC 8239, CCUG 18370, CIP 104880, CECT 486, NRRL B-23851, BCRC 17462, CCRC 17462

Other strains from Clostridium perfringens

Section

Clostridium perfringens CIP 104880 is an anaerobe, spore-forming, mesophilic bacterium of the family Clostridiaceae.

Gram-positive
spore-forming
anaerobe
mesophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium perfringens**
	Full Scientific Name (LPSN)	Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)

Synonym

"Bacillus perfringens"

Information on morphological and physiological properties Morphology

[Ref.: #39127]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #39127]	Cell shape	rod-shaped

[Ref.: #39127]	Motility	no

[Ref.: #39127]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #39127]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #39127]

Culture medium growth

[Ref.: #39127]

Culture medium composition

expand / minimize

[Ref.: #39127]

Culture medium

CIP Medium 20

[Ref.: #39127]

Culture medium growth

[Ref.: #39127]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #39127]			growth	37 °C
[Ref.: #46812]			growth	37 °C

[Ref.: #39127]	Temperature range	mesophilic
[Ref.: #46812]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #46812]	Oxygen tolerance	anaerobe
[Ref.: #39127]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.029

[Ref.: #39127]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.999

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #39127]	D-fructose ChEBI	+/-	degradation
[Ref.: #39127]	D-glucose ChEBI	+/-	degradation
[Ref.: #39127]	esculin ChEBI	+	hydrolysis
[Ref.: #39127]	lactose ChEBI	+/-	degradation
[Ref.: #39127]	maltose ChEBI	+/-	degradation
[Ref.: #39127]	nitrate ChEBI	-	reduction
[Ref.: #39127]	nitrate ChEBI	+	respiration
[Ref.: #39127]	nitrite ChEBI	+	reduction
[Ref.: #39127]	sucrose ChEBI	+/-	degradation

	Metabolite production	Metabolite	Production
[Ref.: #39127]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #39127]	amylase	+
[Ref.: #39127]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #39127]	caseinase	+	3.4.21.50
[Ref.: #39127]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #39127]	DNase	+
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #39127]	gelatinase	+/-
[Ref.: #39127]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #39127]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #39127]	oxidase	-
[Ref.: #39127]	protease	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #39127]	tween esterase	+
[Ref.: #39127]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #39127]	8269	-	+	+	+	-	+	-	-	+	-	+	+	+	+	-	+	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #46812]	Sample type/isolated from	Salt beef,boiled
[Ref.: #46812]	Sampling date	1952
[Ref.: #46812]	Geographic location (country and/or sea, region)	London
[Ref.: #46812]	Country	United Kingdom
[Ref.: #46812]	Country ISO 3 Code	GBR
[Ref.: #46812]	Continent	Europe

[Ref.: #39127]	Sample type/isolated from	Food, Salt beef
[Ref.: #39127]	Geographic location (country and/or sea, region)	London
[Ref.: #39127]	Country	United Kingdom
[Ref.: #39127]	Country ISO 3 Code	GBR
[Ref.: #39127]	Continent	Europe
[Ref.: #39127]	Isolation date	1952

[Ref.: #39127]	Geographic location (country and/or sea, region)	London
[Ref.: #39127]	Country	United Kingdom
[Ref.: #39127]	Country ISO 3 Code	GBR
[Ref.: #39127]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Treatment	#Heated (Burned)
#Engineered	#Food production	#Meat
#Condition	#Saline	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #39127]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium perfringens NCTC 8239	451757.18	wgs	PATRIC	451757 tax ID	*
[Ref.: #66792]	Clostridium perfringens NCTC 8239	GCA_902459515	contig	GenBank	451757 tax ID	*
[Ref.: #66792]	Clostridium perfringens NCTC 8239 str. NCTC 8239	GCA_000171215	contig	GenBank	451757 tax ID	*
[Ref.: #66792]	Clostridium perfringens NCTC 8239	451757.16	wgs	PATRIC	451757 tax ID	*
[Ref.: #66792]	Clostridium perfringens NCTC 8239 strain NCTC8239	451757.9	wgs	PATRIC	451757 tax ID	*
[Ref.: #66792]	Clostridium perfringens NCTC 8239 strain str. NCTC8239	451757.5	wgs	PATRIC	451757 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium perfringens NCTC 8239 strain str. NCTC8239 PATRIC: 451757.5
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium perfringens NCTC 8239 PATRIC: 451757.16
[Ref.: #69480]	gram-positive	yes	95.066	no
[Ref.: #69480]	anaerobic	yes	97.871	no
[Ref.: #69480]	halophile	no	65.698	no
[Ref.: #69480]	spore-forming	yes	90.224	no
[Ref.: #69480]	glucose-util	yes	90.523	no
[Ref.: #69480]	aerobic	no	95.683	yes
[Ref.: #69480]	flagellated	no	91.26	no
[Ref.: #69480]	motile	no	82.092	no
[Ref.: #69480]	thermophile	no	97.788	yes
[Ref.: #69480]	glucose-ferment	yes	77.484	no

Availability in culture collections External links

[Ref.: #39127]

Culture collection no.

CIP 104880, ATCC 12917, NCTC 8239, CCUG 18370

[Ref.: #94911]

SI-ID 35683

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Biotechnology	Predictive model for growth of Clostridium perfringens during cooling of cooked pork supplemented with sodium chloride and sodium pyrophosphate.	Juneja VK, Osoria M, Purohit AS, Golden CE, Mishra A, Taneja NK, Salazar JK, Thippareddi H, Kumar GD	Meat Sci	10.1016/j.meatsci.2021.108557	2021	*
Metabolism	Phosphorothioation of foreign DNA influences the transformation efficiency in Clostridium perfringens NCTC8239.	Yasugi M, Motooka D, Nakamura S, Miyake M	Anaerobe	10.1016/j.anaerobe.2019.102085	2019	*
Pathogenicity	Nitrate salts suppress sporulation and production of enterotoxin in Clostridium perfringens strain NCTC8239.	Yasugi M, Otsuka K, Miyake M	Microbiol Immunol	10.1111/1348-0421.12437	2016	*
Pathogenicity	Transcriptional Profile during Deoxycholate-Induced Sporulation in a Clostridium perfringens Isolate Causing Foodborne Illness.	Yasugi M, Okuzaki D, Kuwana R, Takamatsu H, Fujita M, Sarker MR, Miyake M	Appl Environ Microbiol	10.1128/AEM.00252-16	2016	*
Pathogenicity	Potential for growth of Clostridium perfringens from spores in pork scrapple during cooling.	Juneja VK, Porto-Fett AC, Gartner K, Tufft L, Luchansky JB	Foodborne Pathog Dis	10.1089/fpd.2009.0405	2010	*
Biotechnology	Predictive model for Clostridium perfringens growth in roast beef during cooling and inhibition of spore germination and outgrowth by organic acid salts.	Sanchez-Plata MX, Amezquita A, Blankenship E, Burson DE, Juneja V, Thippareddi H	J Food Prot	10.4315/0362-028x-68.12.2594	2005	*
Pathogenicity	Effect of spices and organic acids on the growth of Clostridium perfringens during cooling of cooked ground beef.	Sabah JR, Juneja VK, Fung DY	J Food Prot	10.4315/0362-028x-67.9.1840	2004	*
Biotechnology	Growth of heat-treated enterotoxin-positive Clostridium perfringens and the implications for safe cooling rates.	Andersen KG, Hansen TB, Knochel S	J Food Prot	10.4315/0362-028x-67.1.83	2004	*
Pathogenicity	Use of organic acids for the control of Clostridium perfringens in cooked vacuum-packaged restructured roast beef during an alternative cooling procedure.	Sabah JR, Thippareddi H, Marsden JL, Fung DY	J Food Prot	10.4315/0362-028x-66.8.1408	2003	*
Biotechnology	Effects of chilling rate on outgrowth of Clostridium perfringens spores in vacuum-packaged cooked beef and pork.	Danler RJ, Boyle EA, Kastner CL, Thippareddi H, Fung DY, Phebus RK	J Food Prot	10.4315/0362-028x-66.3.501	2003	*
Biotechnology	Cooling rate effect on outgrowth of Clostridium perfringens in cooked, ready-to-eat turkey breast roasts.	Steele FM, Wright KH	Poult Sci	10.1093/ps/80.6.813	2001	*
Pathogenicity	Characteristics of a sporulation stimulating factor from Clostridium perfringens type A.	Tseng W, Labbe RG	Lett Appl Microbiol	10.1046/j.1472-765x.2000.00710.x	2000	*
Pathogenicity	The Clostridium perfringens enterotoxin gene is on a transposable element in type A human food poisoning strains.	Brynestad S, Synstad B, Granum PE	Microbiology (Reading)	10.1099/00221287-143-7-2109	1997	*
Phylogeny	Regulated expression of Clostridium perfringens enterotoxin in naturally cpe-negative type A, B, and C isolates of C. perfringens.	Czeczulin JR, Collie RE, McClane BA	Infect Immun	10.1128/iai.64.8.3301-3309.1996	1996	*
Genetics	A complex array of Hpr consensus DNA recognition sequences proximal to the enterotoxin gene in Clostridium perfringens type A.	Brynestad S, Iwanejko LA, Stewart GS, Granum PE	Microbiology (Reading)	10.1099/13500872-140-1-97	1994	*
Pathogenicity	Growth, sporulation and enterotoxin production by Clostridium perfringens type A in the presence of human bile salts.	Heredia NL, Labbe RG, Rodriguez MA, Garcia-Alvarado JS	FEMS Microbiol Lett	10.1016/0378-1097(91)90387-p	1991	*
Metabolism	Characterization of a parasporal inclusion body from sporulating, enterotoxin-positive Clostridium perfringens type A.	Loffler A, Labbe R	J Bacteriol	10.1128/jb.165.2.542-548.1986	1986	*
Biotechnology	Relationship of sporulation, enterotoxin formation, and spoilage during growth of Clostridium perfringens type A in cooked chicken.	Craven SE, Blankenship LC, McDonel JL	Appl Environ Microbiol	10.1128/aem.41.5.1184-1191.1981	1981	*
Metabolism	Growth, sporulation, and germination of Clostridium perfringens in media of controlled water activity.	Kang CK, Woodburn M, Pagenkopf A, Cheney R	Appl Microbiol	10.1128/am.18.5.798-805.1969	1969	*
Phylogeny	Improved isolation of Clostridium perfringens from foal faeces.	Netherwood T, Chanter N, Mumford JA	Res Vet Sci	10.1016/s0034-5288(96)90090-9	1996	*
	Influence of Cooling Rate on Outgrowth of Clostridium perfringens Spores in Cooked Ground Beef.	Juneja VK, Snyder OP Jr, Cygnarowicz-Provost M	J Food Prot	10.4315/0362-028X-57.12.1063	1994	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39127 Collection of Institut Pasteur ; Curators of the CIP; CIP 104880
#46812 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 18370

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#94911

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35683.1 )

* These data were automatically processed and therefore are not curated

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Clostridium perfringens NCTC 8239 strain str. NCTC8239

Clostridium perfringens NCTC 8239

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