Name: Clostridium perfringens McClung 1988
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2632

Type strain:

Species: Clostridium perfringens

Strain Designation: McClung 1988, BP6K

Culture col. no.: CCUG 35750, CIP 104612, BCRC 10648, NCIMB 8875, CECT 4647, DSM 798, ATCC 10543, NCIB 8875, WDCM 00174

Strain history: CIP <- 1995, ATCC <- M.J. Boyd: strain BP6K <- NIH

NCBI tax ID(s): 1502 (species)

SI-ID 35240

ATCC 10543, CCUG 35750, CIP 104612, NCIB 8875, CECT 4647, CCRC 10648, NCIMB 8875, BCRC 10648, DSM 798

Special Collections

Other strains from Clostridium perfringens

Section

Clostridium perfringens McClung 1988 is an anaerobe, spore-forming, Gram-positive human pathogen of the family Clostridiaceae.

Gram-positive
spore-forming
anaerobe
human pathogen
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium perfringens**
	Full Scientific Name (LPSN)	Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)

Synonym

"Bacillus perfringens"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #454]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #454]

Culture medium growth

[Ref.: #454]

Culture medium link

Medium recipe at MediaDive

[Ref.: #454]

Culture medium composition

expand / minimize

[Ref.: #41813]

Culture medium

MEDIUM 20 - for Anaerobic bacteria

[Ref.: #41813]

Culture medium growth

[Ref.: #41813]

Culture medium composition

expand / minimize

[Ref.: #121678]

Culture medium

CIP Medium 20

[Ref.: #121678]

Culture medium growth

[Ref.: #121678]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #454]	growth	37 °C
[Ref.: #41813]	growth	37 °C
[Ref.: #52962]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #454]	Oxygen tolerance	anaerobe
[Ref.: #52962]	Oxygen tolerance	anaerobe

[Ref.: #69481]

Ability of spore formation

yes Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	+	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	+	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	-	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	+	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	+	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	-		* from API rID32A
[Ref.: #68380]	leucine arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	tyrosine arylamidase	-		* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #52962]

Fatty acid	Percentage	ECL
C12:0	21.9	12
C14:0	12	14
C18:0	10	18
C16:0	9.4	16
C18:2 ω6,9c/C18:0 ANTE	7.9	17.724
C19:1 ISO I	5.4	18.473
C17:1 ISO I/C16:0 DMA	5.3	16.481
C20:0	5.1	20
C13:0	3.6	13
C20:2 ω6,9c	3.2	19.735
C19:0 CYCLO ω9c	3	18.87
C18:1 ω9c	2.9	17.769
C13:0 3OH/C15:1 i I/H	2.6	14.469
C20:1 ω7c	1.9	19.833
unknown 16.972	1.9	16.972
C19:0	1.7	19
C11:0	1.2	11
C15:0	1.1	15

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #52962]	4908	-	+	+	+	-	+	-	-	-	+	+	+	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #454]	Pathogenicity (human)	yes
[Ref.: #454]	Pathogenicity (animal)	yes
[Ref.: #454]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121678]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Clostridium perfringens strain ATCC 10543 16S ribosomal RNA gene, partial sequence	GQ911558	771	GenBank ENA	1502 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium perfringens strain FDAARGOS_904	1502.1788	complete	PATRIC	1502 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium perfringens strain FDAARGOS_904 strain Not applicable PATRIC: 1502.1788
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium perfringens strain FDAARGOS_904 strain Not applicable PATRIC: 1502.1788
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	73.511	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	85.433	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	77.5	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	91.38	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.376	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	65.205	no

Availability in culture collections External links

[Ref.: #454]

Culture collection no.

CCUG 35750, CIP 104612, BCRC 10648, NCIMB 8875, CECT 4647, DSM 798, ATCC 10543, NCIB 8875, WDCM 00174

[Ref.: #72163]

SI-ID 35240

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	A clostridial endo-beta-galactosidase that cleaves both blood group A and B glycotopes: the first member of a new glycoside hydrolase family, GH98.	Anderson KM, Ashida H, Maskos K, Dell A, Li SC, Li YT	J Biol Chem	10.1074/jbc.M414099200	2004	*
Enzymology	Characterization of a novel endo-beta-galactosidase specific for releasing the disaccharide GlcNAc alpha 1-->4Gal from glycoconjugates.	Ashida H, Maskos K, Li SC, Li YT	Biochemistry	10.1021/bi011940e	2002	*
Metabolism	A novel endo-beta-galactosidase from Clostridium perfringens that liberates the disaccharide GlcNAcalpha 1-->Gal from glycans specifically expressed in the gastric gland mucous cell-type mucin.	Ashida H, Anderson K, Nakayama J, Maskos K, Chou CW, Cole RB, Li SC, Li YT	J Biol Chem	10.1074/jbc.M103589200	2001	*
Enzymology	Small neuraminidase gene of Clostridium perfringens ATCC 10543: cloning, nucleotide sequence, and production.	Chien CH, Huang YC, Chen HY	Enzyme Microb Technol	10.1016/s0141-0229(96)00129-9	1997	*
Enzymology	Site-directed mutations of the catalytic and conserved amino acids of the neuraminidase gene, nanH, of Clostridium perfringens ATCC 10543.	Chien CH, Shann YJ, Sheu SY	Enzyme Microb Technol	10.1016/0141-0229(95)00245-6	1996	*
Enzymology	Purification and characterization of neuraminidase from Clostridium perfringens.	Chien CH, Chang SC, Wei YH	Proc Natl Sci Counc Repub China B		1989	*
Pathogenicity	Response of white mice to cells and culture constituents of Clostridium perfringens.	Satterlee LD, Walker HW	Appl Microbiol	10.1128/am.18.2.240-244.1969	1969	*

References

#454	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 798

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41813 ; Curators of the CIP;
#52962 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 35750

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72163

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35240.1 )

#121678 Collection of Institut Pasteur ; Curators of the CIP; CIP 104612
* These data were automatically processed and therefore are not curated

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