Strain identifier

BacDive ID: 2632

Type strain: No

Species: Clostridium perfringens

Strain Designation: McClung 1988, BP6K

Strain history: CIP <- 1995, ATCC <- M.J. Boyd: strain BP6K <- NIH

NCBI tax ID(s): 1502 (species)

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General

@ref: 454

BacDive-ID: 2632

DSM-Number: 798

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, mesophilic, Gram-positive, human pathogen

description: Clostridium perfringens McClung 1988 is an anaerobe, spore-forming, mesophilic human pathogen of the family Clostridiaceae.

NCBI tax id

  • NCBI tax id: 1502
  • Matching level: species

strain history

@refhistory
454<- ATCC <- M.J. Boyd, BP6K <- NIH
121678CIP <- 1995, ATCC <- M.J. Boyd: strain BP6K <- NIH

doi: 10.13145/bacdive2632.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium perfringens
  • full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus perfringens

@ref: 454

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium perfringens

full scientific name: Clostridium perfringens (Veillon and Zuber 1898) Hauduroy et al. 1937

strain designation: McClung 1988, BP6K

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
454CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://mediadive.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
41813MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
121678CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
454positivegrowth37mesophilic
41813positivegrowth37mesophilic
52962positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
454anaerobe
52962anaerobe
69480anaerobe98.973

spore formation

@refspore formationconfidence
69481yes100
69480yes99.999

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose+fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate+reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase+3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase+3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase+3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    52962C11:01.211
    52962C12:021.912
    52962C13:03.613
    52962C14:01214
    52962C15:01.115
    52962C16:09.416
    52962C18:01018
    52962C19:01.719
    52962C20:05.120
    52962C13:0 3OH/C15:1 i I/H2.614.469
    52962C17:1 ISO I/C16:0 DMA5.316.481
    52962C18:1 ω9c2.917.769
    52962C18:2 ω6,9c/C18:0 ANTE7.917.724
    52962C19:0 CYCLO ω9c318.87
    52962C19:1 ISO I5.418.473
    52962C20:1 ω7c1.919.833
    52962C20:2 ω6,9c3.219.735
    52962unknown 16.9721.916.972
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
52962-+++-+---+++-++-+-----+------

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
454yesyes2Risk group (German classification)
1216782Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Clostridium perfringens strain ATCC 10543 16S ribosomal RNA gene, partial sequence
  • accession: GQ911558
  • length: 771
  • database: ena
  • NCBI tax ID: 1502

Genome sequences

  • @ref: 66792
  • description: Clostridium perfringens strain FDAARGOS_904
  • accession: 1502.1788
  • assembly level: complete
  • database: patric
  • NCBI tax ID: 1502

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
motileno77.426no
gram-positiveyes93.97no
anaerobicyes97.583no
aerobicno95.73no
halophileno77.278no
spore-formingyes92.577no
glucose-utilyes90.57no
flagellatedno90.528no
thermophileno99.339no
glucose-fermentyes74.593no

External links

@ref: 454

culture collection no.: CCUG 35750, CIP 104612, BCRC 10648, NCIMB 8875, CECT 4647, DSM 798, ATCC 10543, NCIB 8875, WDCM 00174

straininfo link

  • @ref: 72163
  • straininfo: 35240

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Enzymology2556722Purification and characterization of neuraminidase from Clostridium perfringens.Chien CH, Chang SC, Wei YHProc Natl Sci Counc Repub China B1989Clostridium perfringens/*enzymology/growth & development, Enzyme Stability, Hydrogen-Ion Concentration, Kinetics, Molecular Weight, Neuraminidase/*isolation & purification, Substrate SpecificityPhylogeny
Pathogenicity4308935Response of white mice to cells and culture constituents of Clostridium perfringens.Satterlee LD, Walker HWAppl Microbiol1969Animal Feed, Animals, Chemical Precipitation, Chromatography, Clostridium Infections/*etiology, *Clostridium perfringens/analysis, Foodborne Diseases/*etiology, Gastrointestinal Motility/drug effects, Mice, Neuraminidase/analysis/pharmacology, Phospholipases/analysis/pharmacology, Toxins, Biological/analysis10.1128/am.18.2.240-244.1969
Enzymology8987487Site-directed mutations of the catalytic and conserved amino acids of the neuraminidase gene, nanH, of Clostridium perfringens ATCC 10543.Chien CH, Shann YJ, Sheu SYEnzyme Microb Technol1996Amino Acid Sequence, Base Sequence, Binding Sites/physiology, Blotting, Western, Chemical Phenomena, Chemistry, Cloning, Molecular, Clostridium perfringens/*enzymology, Conserved Sequence/genetics, DNA Primers, Genes, Bacterial/*genetics, Kinetics, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed/genetics, Mutation/genetics, N-Acetylneuraminic Acid/metabolism, Neuraminidase/*genetics, Precipitin Tests, Protein Conformation, Sequence AnalysisGenetics10.1016/0141-0229(95)00245-6
Enzymology9115702Small neuraminidase gene of Clostridium perfringens ATCC 10543: cloning, nucleotide sequence, and production.Chien CH, Huang YC, Chen HYEnzyme Microb Technol1997Amino Acid Sequence, Base Sequence, Biotechnology, Cloning, Molecular, Clostridium perfringens/*enzymology/*genetics, DNA, Bacterial/genetics, Escherichia coli/genetics, Gene Expression, *Genes, Bacterial, Molecular Sequence Data, Neuraminidase/biosynthesis/*genetics, Salmonella typhimurium/enzymology/genetics, Transformation, GeneticBiotechnology10.1016/s0141-0229(96)00129-9
Metabolism11382776A novel endo-beta-galactosidase from Clostridium perfringens that liberates the disaccharide GlcNAcalpha 1-->Gal from glycans specifically expressed in the gastric gland mucous cell-type mucin.Ashida H, Anderson K, Nakayama J, Maskos K, Chou CW, Cole RB, Li SC, Li YTJ Biol Chem2001Adenocarcinoma/metabolism, Ammonium Sulfate/metabolism, Animals, Chromatography, Affinity, Clostridium perfringens/*enzymology, DNA, Complementary/metabolism, Disaccharides/*chemistry/metabolism, Electrophoresis, Polyacrylamide Gel, Epitopes, Gastric Mucosa/*metabolism, *Glycoside Hydrolases, Humans, Hydrogen-Ion Concentration, Hydrolysis, Immunoblotting, Immunohistochemistry, Magnetic Resonance Spectroscopy, Models, Chemical, Mucins/*metabolism, Spectrometry, Mass, Electrospray Ionization, Stomach Neoplasms/embryology, Substrate Specificity, Swine, Transfection, Tumor Cells, Cultured, beta-Galactosidase/*chemistry/metabolismEnzymology10.1074/jbc.M103589200
Enzymology11841232Characterization of a novel endo-beta-galactosidase specific for releasing the disaccharide GlcNAc alpha 1-->4Gal from glycoconjugates.Ashida H, Maskos K, Li SC, Li YTBiochemistry2002Amino Acid Sequence, Base Sequence, Catalytic Domain/genetics, Cloning, Molecular, Clostridium perfringens/enzymology/genetics, Disaccharides/*metabolism, Escherichia coli/enzymology/genetics, Gene Expression Regulation, Enzymologic, Glycoconjugates/*metabolism, *Glycoside Hydrolases, Molecular Sequence Data, Mutagenesis, Site-Directed, Nuclear Magnetic Resonance, Biomolecular, Sequence Homology, Amino Acid, Substrate Specificity/genetics, beta-Galactosidase/biosynthesis/*chemistry/genetics/*metabolismMetabolism10.1021/bi011940e
Enzymology15618227A clostridial endo-beta-galactosidase that cleaves both blood group A and B glycotopes: the first member of a new glycoside hydrolase family, GH98.Anderson KM, Ashida H, Maskos K, Dell A, Li SC, Li YTJ Biol Chem2004ABO Blood-Group System, Amino Acid Sequence, Animals, Base Sequence, Cell Separation, Chromatography, Chromatography, Gel, Chromatography, Thin Layer, Cloning, Molecular, Clostridium perfringens/*enzymology, Concanavalin A/pharmacology, DNA Primers/chemistry, Databases as Topic, Electrophoresis, Polyacrylamide Gel, Erythrocytes/metabolism, Escherichia coli/enzymology, Female, Flow Cytometry, Glycoproteins/chemistry, Glycoside Hydrolases/*chemistry, Humans, Hydrolysis, Lectins/chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Ovarian Cysts/metabolism, Peptides/chemistry, Plasmids/metabolism, Polysaccharides/chemistry, Recombinant Proteins/chemistry, Swine, Time FactorsMetabolism10.1074/jbc.M414099200

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
454Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 798)https://www.dsmz.de/collection/catalogue/details/culture/DSM-798
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41813Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16540
52962Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 35750)https://www.ccug.se/strain?id=35750
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72163Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35240.1StrainInfo: A central database for resolving microbial strain identifiers
121678Curators of the CIPCollection of Institut Pasteur (CIP 104612)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104612