Name: Bacillus cereus F 4433/73
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 622

Type strain:

Species: Bacillus cereus

Strain Designation: F 4433/73

Culture col. no.: DSM 4313, NCTC 11145, CCUG 6514, CIP 106508, ATCC 49064

Strain history: CIP <- 2000, CCUG <- NCTC, London, UK <- R.J. Gilbert, FHL, PHLS, Colindale <- J.M. Goepfert <- T.F. Midura, USA: Strain F4433/73

NCBI tax ID(s): 1396 (species)

SI-ID 7075

LMG 12335, DSM 4313, NCTC 11145, ATCC 49064, CCUG 6514, CIP 106508, BCRC 15324, CCRC 15324

Special Collections

Other strains from Bacillus cereus

Section

Bacillus cereus F 4433/73 is a facultative anaerobe, spore-forming, Gram-positive bacterium that was isolated from Meat loaf implicated in gastroenteritis outbreak.

Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus cereus**
	Full Scientific Name (LPSN)	Bacillus cereus Frankland and Frankland 1887 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #118206]	Gram stain	positive

[Ref.: #118206]	Cell shape	rod-shaped

[Ref.: #118206]	Motility	yes

[Ref.: #118206]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #33726]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #33726]

Culture medium growth

[Ref.: #33726]

Culture medium composition

expand / minimize

[Ref.: #1653]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1653]

Culture medium growth

[Ref.: #1653]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1653]

Culture medium composition

expand / minimize

[Ref.: #118206]

Culture medium

CIP Medium 72

[Ref.: #118206]

Culture medium growth

[Ref.: #118206]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #118206]

Culture medium

CIP Medium 3

[Ref.: #118206]

Culture medium growth

[Ref.: #118206]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #1653]		growth	30 °C
[Ref.: #33726]		growth	30 °C
[Ref.: #118206]		growth	22-45 °C
[Ref.: #118206]	no	growth	10 °C
[Ref.: #118206]	no	growth	55 °C

	pH	Kind of pH		pH
[Ref.: #118206]			growth	6

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118206]

Oxygen tolerance

facultative anaerobe

[Ref.: #118206]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #118206]	NaCl		growth	0-6	%
[Ref.: #118206]	NaCl	no	growth	8	%
[Ref.: #118206]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #118206]	citrate ChEBI	+	carbon source
[Ref.: #118206]	esculin ChEBI	+	hydrolysis
[Ref.: #118206]	nitrate ChEBI	+	reduction
[Ref.: #118206]	nitrate ChEBI	+	respiration
[Ref.: #118206]	nitrite ChEBI	+	reduction

	Metabolite production	Metabolite	Production
[Ref.: #118206]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #118206]		acetoin ChEBI	+
[Ref.: #118206]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118206]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #118206]	amylase	+
[Ref.: #118206]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118206]	caseinase	+	3.4.21.50
[Ref.: #118206]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #118206]	DNase	+
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #118206]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #118206]	gelatinase	+
[Ref.: #118206]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #118206]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118206]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118206]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118206]	oxidase	-
[Ref.: #118206]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118206]	tween esterase	-
[Ref.: #118206]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #118206]	1727	not determinedn.d.	+	+	-	+	+	-	-	-	-	+	+	-	-	-	-	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	+	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118206]	9566	-	+	-	+	-	+	+	+	-	+	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44905]	Sample type/isolated from	Meat loaf implicated in gastroenteritis outbreak
[Ref.: #44905]	Geographic location (country and/or sea, region)	California
[Ref.: #44905]	Country	USA
[Ref.: #44905]	Country ISO 3 Code	USA
[Ref.: #44905]	Continent	North America

[Ref.: #118206]	Sample type/isolated from	Food, Meat loaf implicated in gastroenteritis outbreak
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Meat
#Infection	#Disease	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1653]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #118206]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus cereus DSM 4313	GCA_018221725	contig	GenBank ENA	1396 tax ID	*
[Ref.: #66792]	Bacillus cereus strain DSM 4313	1396.2855	wgs	PATRIC	1396 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus cereus strain DSM 4313 PATRIC: 1396.2855
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus cereus strain DSM 4313 PATRIC: 1396.2855
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	74.972	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.046	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	83.183	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	57.759	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94.51	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	85.431	no

Availability in culture collections External links

[Ref.: #1653]

Culture collection no.

DSM 4313, NCTC 11145, CCUG 6514, CIP 106508, ATCC 49064

[Ref.: #70298]

SI-ID 7075

Literature:

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Enhancement of mechanical properties, microstructure, and antimicrobial activities of zein films cross-linked using succinic anhydride, eugenol, and citric Acid.	Khalil AA, Deraz SF, Elrahman SA, El-Fawal G	Prep Biochem Biotechnol	10.1080/10826068.2014.940967	2015	*
Pathogenicity	Enterotoxigenicity and cytotoxicity of Bacillus thuringiensis strains and development of a process for Cry1Ac production.	Yang CY, Pang JC, Kao SS, Tsen HY	J Agric Food Chem	10.1021/jf025863l	2003	*
Enzymology	Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. 'Liscia' and C. medica cv. 'Rugosa' Cultivated in Southern Italy.	Aliberti L, Caputo L, De Feo V, De Martino L, Nazzaro F, Souza LF	Molecules	10.3390/molecules21091244	2016	*
Enzymology	Chemical Composition, Antibacterial and Phytotoxic Activities of Peganum harmala Seed Essential Oils from Five Different Localities in Northern Africa.	Apostolico I, Aliberti L, Caputo L, De Feo V, Fratianni F, Nazzaro F, Souza LF, Khadhr M	Molecules	10.3390/molecules21091235	2016	*

References

#1653

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4313

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33726 ; Curators of the CIP;
#44905 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 6514

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#70298

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7075.1 )

#118206 Collection of Institut Pasteur ; Curators of the CIP; CIP 106508
* These data were automatically processed and therefore are not curated

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Bacillus cereus strain DSM 4313

Bacillus cereus strain DSM 4313

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