Strain identifier

BacDive ID: 622

Type strain: No

Species: Bacillus cereus

Strain Designation: F 4433/73

Strain history: CIP <- 2000, CCUG <- NCTC, London, UK <- R.J. Gilbert, FHL, PHLS, Colindale <- J.M. Goepfert <- T.F. Midura, USA: Strain F4433/73

NCBI tax ID(s): 1396 (species)

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General

@ref: 1653

BacDive-ID: 622

DSM-Number: 4313

keywords: genome sequence, Bacteria, facultative anaerobe, spore-forming, Gram-positive, motile, rod-shaped

description: Bacillus cereus F 4433/73 is a facultative anaerobe, spore-forming, Gram-positive bacterium that was isolated from Meat loaf implicated in gastroenteritis outbreak.

NCBI tax id

  • NCBI tax id: 1396
  • Matching level: species

strain history

@refhistory
1653<- NCTC
118206CIP <- 2000, CCUG <- NCTC, London, UK <- R.J. Gilbert, FHL, PHLS, Colindale <- J.M. Goepfert <- T.F. Midura, USA: Strain F4433/73

doi: 10.13145/bacdive622.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus cereus
  • full scientific name: Bacillus cereus Frankland and Frankland 1887 (Approved Lists 1980)

@ref: 1653

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus cereus

full scientific name: Bacillus cereus Frankland and Frankland 1887

strain designation: F 4433/73

type strain: no

Morphology

cell morphology

  • @ref: 118206
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 118206
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
33726MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
1653NUTRIENT AGAR (DSMZ Medium 1)yesName: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1
118206CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
118206CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperature
1653positivegrowth30
33726positivegrowth30
118206positivegrowth22-45
118206nogrowth10
118206nogrowth55

culture pH

  • @ref: 118206
  • ability: positive
  • type: growth
  • pH: 6

Physiology and metabolism

oxygen tolerance

  • @ref: 118206
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore formationconfidence
118206yes
69481yes100

halophily

@refsaltgrowthtested relationconcentration
118206NaClpositivegrowth0-6 %
118206NaClnogrowth8 %
118206NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11820616947citrate+carbon source
1182064853esculin+hydrolysis
11820617632nitrate+reduction
11820616301nitrite+reduction
11820617632nitrate+respiration

metabolite production

  • @ref: 118206
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11820615688acetoin+
11820617234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
118206oxidase-
118206beta-galactosidase-3.2.1.23
118206alcohol dehydrogenase+1.1.1.1
118206gelatinase+
118206amylase+
118206DNase+
118206caseinase+3.4.21.50
118206catalase+1.11.1.6
118206tween esterase-
118206gamma-glutamyltransferase-2.3.2.2
118206lecithinase+
118206lipase+
118206lysine decarboxylase-4.1.1.18
118206ornithine decarboxylase-4.1.1.17
118206protease+
118206urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118206-+-+-+++-+++---++---

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
118206++-++----++----+--++-----------+-----------------++--+--+--++--------------+---++-------+-----+-+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
44905Meat loaf implicated in gastroenteritis outbreakCaliforniaUSAUSANorth America
118206Food, Meat loaf implicated in gastroenteritis outbreak

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Meat
#Infection#Disease

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
16532Risk group (German classification)
1182062Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Bacillus cereus DSM 4313GCA_018221725contigncbi1396
66792Bacillus cereus strain DSM 43131396.2855wgspatric1396

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesyes100no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes74.972no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no92.046no
69480spore-formingspore-formingAbility to form endo- or exosporesyes83.183no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no57.759yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno94.51yes
69480flagellatedmotile2+Ability to perform flagellated movementyes85.431no

External links

@ref: 1653

culture collection no.: DSM 4313, NCTC 11145, CCUG 6514, CIP 106508, ATCC 49064

straininfo link

  • @ref: 70298
  • straininfo: 7075

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity12502392Enterotoxigenicity and cytotoxicity of Bacillus thuringiensis strains and development of a process for Cry1Ac production.Yang CY, Pang JC, Kao SS, Tsen HYJ Agric Food Chem10.1021/jf025863l2003Animals, Bacillus thuringiensis/genetics/metabolism/*pathogenicity, Bacillus thuringiensis Toxins, Bacterial Proteins/*biosynthesis/genetics, *Bacterial Toxins, CHO Cells/drug effects, Cell Death/drug effects, Cricetinae, Endotoxins/*biosynthesis/genetics, Enterotoxins/biosynthesis, Gene Expression, Genetic Vectors, Hemolysin Proteins, Insecticides, Pest Control, Biological, Promoter Regions, Genetic, Recombinant Proteins, alpha-Amylases/geneticsBiotechnology
Pathogenicity25036665Enhancement of mechanical properties, microstructure, and antimicrobial activities of zein films cross-linked using succinic anhydride, eugenol, and citric Acid.Khalil AA, Deraz SF, Elrahman SA, El-Fawal GPrep Biochem Biotechnol10.1080/10826068.2014.9409672015Anti-Bacterial Agents/chemical synthesis/pharmacology, Bacterial Physiological Phenomena/*drug effects, Cell Survival/drug effects, Citric Acid/*chemistry, Cross-Linking Reagents/chemistry, Elastic Modulus, Eugenol/*chemistry, Materials Testing, *Membranes, Artificial, Succinic Anhydrides/*chemistry, Surface Properties, Tensile Strength, Zein/*chemistry/*pharmacology/ultrastructure
Enzymology27649128Chemical Composition, Antibacterial and Phytotoxic Activities of Peganum harmala Seed Essential Oils from Five Different Localities in Northern Africa.Apostolico I, Aliberti L, Caputo L, De Feo V, Fratianni F, Nazzaro F, Souza LF, Khadhr MMolecules10.3390/molecules210912352016Africa, Northern, *Anti-Bacterial Agents/chemistry/isolation & purification/pharmacology, Bacteria/*growth & development, *Herbicides/chemistry/isolation & purification/pharmacology, Lepidium sativum/*growth & development, *Oils, Volatile/chemistry/isolation & purification/pharmacology, Peganum/*chemistry, Raphanus/*growth & development, Ruta/*growth & development, Seeds/*chemistryPhylogeny
Enzymology27649138Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. 'Liscia' and C. medica cv. 'Rugosa' Cultivated in Southern Italy.Aliberti L, Caputo L, De Feo V, De Martino L, Nazzaro F, Souza LFMolecules10.3390/molecules210912442016Adenylyl Cyclases/biosynthesis, *Anti-Infective Agents/chemistry/isolation & purification/pharmacology, Bacteria/*growth & development, Cell Line, Tumor, *Central Nervous System Agents/chemistry/isolation & purification/pharmacology, Citrus/*chemistry, *Cytotoxins/chemistry/isolation & purification/pharmacology, Fruit/*chemistry, Humans, Italy, *Oils, Volatile/chemistry/isolation & purification/pharmacologyPhylogeny

Reference

@idauthorscataloguedoi/urltitle
1653Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 4313)https://www.dsmz.de/collection/catalogue/details/culture/DSM-4313
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33726Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18647
44905Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 6514)https://www.ccug.se/strain?id=6514
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
70298Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7075.1StrainInfo: A central database for resolving microbial strain identifiers
118206Curators of the CIPCollection of Institut Pasteur (CIP 106508)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106508