Name: Mycobacterium avium subsp. paratuberculosis DSM 44133
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8439

Type strain:

Species: Mycobacterium avium subsp. paratuberculosis

Culture col. no.: DSM 44133, ATCC 19698, TMC 807, CCUG 55483, CIP 103963

Strain history: CIP <- 1994, ATCC <- R.S. Merkal: strain 5617, Mycobacterium paratuberculosis <- D. Porter: strain TMC 807

NCBI tax ID(s): 1074454 (strain), 1770 (subspecies)

SI-ID 44555

ATCC 19698, CIP 103963, DSM 44133, CCUG 55483

Other strains from Mycobacterium avium subsp. paratuberculosis

Section

Mycobacterium avium subsp. paratuberculosis DSM 44133 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from feces of cow with naturally aquired paratuberculosis.

Gram-positive
rod-shaped
human pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium avium subsp. paratuberculosis**
	Full Scientific Name (LPSN)	Mycobacterium avium subsp. paratuberculosis (Bergey et al. 1923) Thorel et al. 1990

Synonym

Mycobacterium paratuberculosis

Information on morphological and physiological properties Morphology

[Ref.: #116671]	Gram stain	positive

[Ref.: #116671]	Cell shape	rod-shaped

[Ref.: #116671]	Motility	no

[Ref.: #60601]

Incubation period

30 days

Multimedia content

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Intellectual property rights:

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Caption:

Medium 780 37°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

MIDDLEBROOK MEDIUM WITH MYCOBACTIN (DSMZ Medium 780)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

MEDIUM 72- for trypto casein soja agar

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

CIP Medium 96

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

CIP Medium 72

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #11514]	growth	37 °C
[Ref.: #40896]	growth	37 °C
[Ref.: #60601]	growth	37 °C
[Ref.: #116671]	growth	37 °C

[Ref.: #11514]	Temperature range	mesophilic
[Ref.: #40896]	Temperature range	mesophilic
[Ref.: #60601]	Temperature range	mesophilic
[Ref.: #116671]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #60601]

Oxygen tolerance

microaerophile

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116671]		nitrate ChEBI	-	reduction
[Ref.: #116671]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #116671]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116671]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #116671]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116671]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116671]	7751	-	+	+	+	-	+	-	-	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11514]	Sample type/isolated from	feces of cow with naturally aquired paratuberculosis
[Ref.: #11514]	Country	USA
[Ref.: #11514]	Country ISO 3 Code	USA
[Ref.: #11514]	Continent	North America

[Ref.: #60601]	Sample type/isolated from	Cow feces,naturally aquired paratuberculos
[Ref.: #60601]	Sampling date	1966
[Ref.: #60601]	Geographic location (country and/or sea, region)	Iowa
[Ref.: #60601]	Country	USA
[Ref.: #60601]	Country ISO 3 Code	USA
[Ref.: #60601]	Continent	North America

[Ref.: #116671]	Sample type/isolated from	Animal, Cow, feces
[Ref.: #116671]	Geographic location (country and/or sea, region)	Ames, Iowa
[Ref.: #116671]	Country	United States of America
[Ref.: #116671]	Country ISO 3 Code	USA
[Ref.: #116671]	Continent	North America
[Ref.: #116671]	Isolation date	1966
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X52934 (>99% sequence identity) for Mycobacterium from Microbeatlas

Aquatic Samples	4789
Soil Samples	12779
Animal Samples	3677
Plant Samples	2833
Total Samples	24078

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11514]	Pathogenicity (human)	yes
[Ref.: #11514]	Pathogenicity (animal)	yes
[Ref.: #11514]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #116671]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11514]	Mycobacterium avium subsp. paratuberculosis strain ATCC 19698 16S ribosomal RNA gene, partial sequence	EF521896	1442	ENA	1770 tax ID
[Ref.: #20218]	Mycobacterium avium subsp. paratuberculosis strain ATCC 19698 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence	EF521900	438	ENA	1770 tax ID	*
[Ref.: #20218]	M.paratuberculosis 16S ribosomal RNA gene	M59265	145	ENA	1770 tax ID	*
[Ref.: #20218]	Mycobacterium paratuberculosis 16S rRNA gene	X52934	1463	ENA	1770 tax ID	*
[Ref.: #20218]	Mycobacterium avium subsp. paratuberculosis strain CIP 103963 16S ribosomal RNA gene, partial sequence	AF547899	554	ENA	1770 tax ID	*
[Ref.: #20218]	Mycobacterium avium subsp. paratuberculosis strain DSM 44133 16S ribosomal RNA gene, partial sequence	HM007575	595	ENA	1770 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacterium avium subsp. paratuberculosis ATCC 19698	1074454.6	wgs	PATRIC	1074454 tax ID	*
[Ref.: #66792]	Mycobacterium avium paratuberculosis ATCC 19698	2548876531	draft	JGI IMG/M	1074454 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium avium subsp. paratuberculosis ATCC 19698 PATRIC: 1074454.6
[Ref.: #69480]	motile	no	95.329	no
[Ref.: #69480]	flagellated	no	98.872	no
[Ref.: #69480]	gram-positive	yes	90.117	no
[Ref.: #69480]	anaerobic	no	99.548	no
[Ref.: #69480]	aerobic	yes	91.382	yes
[Ref.: #69480]	halophile	no	92.986	no
[Ref.: #69480]	spore-forming	no	85.72	no
[Ref.: #69480]	thermophile	no	97.386	no
[Ref.: #69480]	glucose-util	yes	63.486	no
[Ref.: #69480]	glucose-ferment	no	92.844	no

Availability in culture collections External links

[Ref.: #11514]

Culture collection no.

DSM 44133, ATCC 19698, TMC 807, CCUG 55483, CIP 103963

[Ref.: #77838]

SI-ID 44555

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Development and validation of a loop-mediated isothermal amplification assay-a rapid and sensitive detection tool for Mycobacterium avium subsp. paratuberculosis in small ruminants.	Sange MD, Becker A, Hassan AA, Bulte M, Ganter M, Siebert U, Abdulmawjood A	J Appl Microbiol	10.1111/jam.14284	2019	*
Genetics	Stability of genotyping target sequences of Mycobacterium avium subsp. paratuberculosis upon cultivation on different media, in vitro- and in vivo passage, and natural infection.	Kasnitz N, Kohler H, Weigoldt M, Gerlach GF, Mobius P	Vet Microbiol	10.1016/j.vetmic.2013.09.008	2013	*
Genetics	Divergent cytokine responses of macrophages to Mycobacterium avium subsp. paratuberculosis strains of Types II and III in a standardized in vitro model.	Borrmann E, Mobius P, Diller R, Kohler H	Vet Microbiol	10.1016/j.vetmic.2011.04.002	2011	*
Pathogenicity	Gene encoded antimicrobial peptides, a template for the design of novel anti-mycobacterial drugs.	Carroll J, Field D, O'Connor PM, Cotter PD, Coffey A, Hill C, Ross RP, O'Mahony J	Bioeng Bugs	10.4161/bbug.1.6.13642	2010	*
Pathogenicity	Comparison of the activities of the lantibiotics nisin and lacticin 3147 against clinically significant mycobacteria.	Carroll J, Draper LA, O'Connor PM, Coffey A, Hill C, Ross RP, Cotter PD, O'Mahony J	Int J Antimicrob Agents	10.1016/j.ijantimicag.2010.03.029	2010	*
Biotechnology	[Study on the effects of HTST pasteurization temperatures on Mycobacterium avium subsp. paratuberculosis in an industrial fluid milk-processing system].	Igimi S, Iriguchi S, Monden S, Okada Y, Yamamoto S, Mori Y	Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku		2010	*
Enzymology	Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea.	Park KT, Ahn J, Davis WC, Koo HC, Kwon NH, Jung WK, Kim JM, Hong SK, Park YH	J Vet Sci	10.4142/jvs.2006.7.4.349	2006	*
Pathogenicity	Interaction between Mycobacterium avium subsp. paratuberculosis and environmental protozoa.	Whan L, Grant IR, Rowe MT	BMC Microbiol	10.1186/1471-2180-6-63	2006	*
Enzymology	Inactivation of Mycobacterium avium subsp. paratuberculosis in cow's milk by means of high hydrostatic pressure at mild temperatures.	Lopez-Pedemonte T, Sevilla I, Garrido JM, Aduriz G, Guamis B, Juste RA, Roig-Sagues AX	Appl Environ Microbiol	10.1128/AEM.01924-05	2006	*
Metabolism	Members of the 30- to 32-kilodalton mycolyl transferase family (Ag85) from culture filtrate of Mycobacterium avium subsp. paratuberculosis are immunodominant Th1-type antigens recognized early upon infection in mice and cattle.	Rosseels V, Marche S, Roupie V, Govaerts M, Godfroid J, Walravens K, Huygen K	Infect Immun	10.1128/IAI.74.1.202-212.2006	2006	*
Phylogeny	Real-time quantitative PCR detection of Mycobacterium avium subsp. paratuberculosis and differentiation from other mycobacteria using SYBR Green and TaqMan assays.	Ravva SV, Stanker LH	J Microbiol Methods	10.1016/j.mimet.2005.04.004	2005	*
Enzymology	Amplified fragment length polymorphism reveals genomic variability among Mycobacterium avium subsp. paratuberculosis isolates.	O'Shea B, Khare S, Bliss K, Klein P, Ficht TA, Adams LG, Rice-Ficht AC	J Clin Microbiol	10.1128/JCM.42.8.3600-3606.2004	2004	*
Cultivation	Effect of turbulent-flow pasteurization on survival of Mycobacterium avium subsp. paratuberculosis added to raw milk.	Pearce LE, Truong HT, Crawford RA, Yates GF, Cavaignac S, de Lisle GW	Appl Environ Microbiol	10.1128/AEM.67.9.3964-3969.2001	2001	*
Cultivation	Effect of three factors in cheese production (pH, salt, and heat) on Mycobacterium avium subsp. paratuberculosis viability.	Sung N, Collins MT	Appl Environ Microbiol	10.1128/AEM.66.4.1334-1339.2000	2000	*
Pathogenicity	HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium.	Dei R, Tortoli E, Bartoloni A, Simonetti MT, Lillini E	Vet Microbiol	10.1016/s0378-1135(98)00292-2	1999	*
Stress	Thermal tolerance of Mycobacterium paratuberculosis.	Sung N, Collins MT	Appl Environ Microbiol	10.1128/AEM.64.3.999-1005.1998	1998	*
Enzymology	Relationship of acid phosphatase activity to ultrastructural features in mice inoculated with Mycobacterium paratuberculosis.	Tanaka S, Sato M, Taniguchi T, Yokomizo Y	J Comp Pathol	10.1016/s0021-9975(96)80065-x	1996	*
Enzymology	Histopathological and morphometrical comparison of granulomatous lesions in BALB/c and C3H/HeJ mice inoculated with Mycobacterium paratuberculosis.	Tanaka S, Sato M, Taniguchi T, Yokomizo Y	J Comp Pathol	10.1016/s0021-9975(08)80315-5	1994	*
Pathogenicity	Effects of colonial morphology and tween 80 on antimicrobial susceptibility of Mycobacterium paratuberculosis.	Van Boxtel RM, Lambrecht RS, Collins MT	Antimicrob Agents Chemother	10.1128/AAC.34.12.2300	1990	*
Metabolism	Granulomatous enteritis following oral inoculation of newborn rabbits with Mycobacterium paratuberculosis of bovine origin.	Mokresh AH, Butler DG	Can J Vet Res		1990	*
Enzymology	Analysis of restriction endonuclease fragment patterns of DNA from Mycobacterium paratuberculosis.	Whipple DL, Kapke PA, Andrews RE Jr	Vet Microbiol	10.1016/0378-1135(89)90084-9	1989	*
Phylogeny	Genetic relatedness among Mycobacterium paratuberculosis and M. avium complex.	Labidi A, Thoen CO	Acta Leprol		1989	*
Enzymology	Characterization of total deoxyribonucleic acid of Mycobacterium paratuberculosis (ATCC 19698) and of M. avium complex (ATCC 25291) using restriction enzymes.	Labidi A	Arch Inst Pasteur Tunis		1988	*
	Development and application of quantitative polymerase chain reaction assay based on the ABI 7700 system (TaqMan) for detection and quantification of Mycobacterium avium subsp. paratuberculosis.	Kim SG, Shin SJ, Jacobson RH, Miller LJ, Harpending PR, Stehman SM, Rossiter CA, Lein DA	J Vet Diagn Invest	10.1177/104063870201400206	2002	*
	Reduced formation of granulomata in gamma(delta) T cell knockout BALB/c mice inoculated with Mycobacterium avium subsp. paratuberculosis.	Tanaka S, Itohara S, Sato M, Taniguchi T, Yokomizo Y	Vet Pathol	10.1354/vp.37-5-415	2000	*
	A rabbit model for study of Mycobacterium paratuberculosis infection.	Mokresh AH, Czuprynski CJ, Butler DG	Infect Immun	10.1128/iai.57.12.3798-3807.1989	1989	*

References

#11514

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44133

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40896 ; Curators of the CIP;
#60601 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55483

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#77838

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44555.1 )

#116671 Collection of Institut Pasteur ; Curators of the CIP; CIP 103963
* These data were automatically processed and therefore are not curated

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Mycobacterium avium subsp. paratuberculosis ATCC 19698

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