Mycobacterium avium subsp. paratuberculosis (CIP 103964, ATCC 19851, NCTC 8578)

Name: Mycobacterium avium subsp. paratuberculosis (CIP 103964, ATCC 19851, NCTC 8578)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 135349

Type strain

Strain Designation JC31

Culture col. no. CIP 103964 ATCC 19851 NCTC 8578 JC31

NCBI tax ID(s) 1770

Special Collections CIP

History CIP <- 1994, ATCC <- NCTC, Mycobacterium johnei <- H.W. Smith: strain JC31

Links

Mycobacterium avium subsp. paratuberculosis JC31 is a rod-shaped bacterium that was isolated from Human, With Johne's disease.

rod-shaped Bacteria

Name and taxonomic classification

SI-ID 37132

ATCC 19851,NCTC 8578,CIP 103964

@ref 20215

Last LPSN update 2026-05-29 08:38:32

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium avium subsp. paratuberculosis

Full scientific name Mycobacterium avium subsp. paratuberculosis (Bergey et al. 1923) Thorel et al. 1990

Synonyms (1)

Mycobacterium paratuberculosis

BacDive ID	Other strains from **Mycobacterium avium subsp. paratuberculosis** (9)	Type strain
8439	M. avium subsp. paratuberculosis DSM 44133, ATCC 19698, TMC 807, CCUG 55483, ... (type strain)
8440	M. avium subsp. paratuberculosis 1351, DSM 44135
135350	M. avium subsp. paratuberculosis CIP 103967, ATCC 43545
135351	M. avium subsp. paratuberculosis CIP 103968, ATCC 49164
135352	M. avium subsp. paratuberculosis 1001, CIP 103971
136665	M. avium subsp. paratuberculosis TB795/95, CIP 107488
136893	M. avium subsp. paratuberculosis CIP 103965, ATCC 43015
137548	M. avium subsp. paratuberculosis 4138, CIP 103978
169420	M. avium subsp. paratuberculosis JIII-386, DSM 108962

Morphology

Cell morphology

@ref	Cell shape	Motility
34445	rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Growth	Composition	Medium link
34445	MEDIUM 96 - for Mycobacterium avium subsp. paratuberculosis		Tween 80 (0.500 ml);Middlebrook 7H9 broth (4.700 g);Middelbrook OADC enrichment (100.000 ml);Distilled water (900.000 ml);Mycobactine (2.000 ml)
34445	CIP Medium 96			Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
34445	positive	growth	37
34445	positive	growth	30-37
34445	negative	growth	10
34445	negative	growth	22
34445	negative	growth	45

Physiology and metabolism

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
34445	606565 ChEBI	hippurate	-	hydrolysis
34445	17632 ChEBI	nitrate	-	reduction
34445	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
34445	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
34445	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
34445	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
34445	gamma-glutamyltransferase	+	2.3.2.2
34445	gelatinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
34445	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
34445	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
34445	-	-	+	+	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Host species
34445	Human, With Johne's disease	Homo sapiens

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
34445	2	Risk group (French classification)

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Estimation of Performance Characteristics of Analytical Methods for Mycobacterium avium subsp. paratuberculosis Detection in Dairy Products.	Butot S, Ricchi M, Sevilla IA, Michot L, Molina E, Tello M, Russo S, Arrigoni N, Garrido JM, Tomas D.	Front Microbiol	10.3389/fmicb.2019.00509	2019
Enzymology	Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria.	Swift BM, Gerrard ZE, Huxley JN, Rees CE.	PLoS One	10.1371/journal.pone.0106690	2014
Enzymology	Development of a new, combined rapid method using phage and PCR for detection and identification of viable Mycobacterium paratuberculosis bacteria within 48 hours.	Stanley EC, Mole RJ, Smith RJ, Glenn SM, Barer MR, McGowan M, Rees CE.	Appl Environ Microbiol	10.1128/aem.01722-06	2007
Phylogeny	Amplified fragment length polymorphism reveals specific epigenetic distinctions between Mycobacterium avium subspecies paratuberculosis isolates of various isolation types.	O'Shea B, Khare S, Klein P, Roussel A, Adams LG, Ficht TA, Rice-Ficht AC.	J Clin Microbiol	10.1128/jcm.01123-10	2011
Phylogeny	Mycobacterium avium subsp. paratuberculosis strains isolated from Crohn's disease patients and animal species exhibit similar polymorphic locus patterns.	Ghadiali AH, Strother M, Naser SA, Manning EJ, Sreevatsan S.	J Clin Microbiol	10.1128/jcm.42.11.5345-5348.2004	2004
Enzymology	Application of the C(18)-carboxypropylbetaine specimen processing method to recovery of Mycobacterium avium subsp. paratuberculosis from ruminant tissue specimens.	Thornton CG, MacLellan KM, Stabel JR, Carothers C, Whitlock RH, Passen S.	J Clin Microbiol	10.1128/jcm.40.5.1783-1790.2002	2002
	Mycobacteriophage cell binding proteins for the capture of mycobacteria.	Arutyunov D, Singh U, El-Hawiet A, Seckler HD, Nikjah S, Joe M, Bai Y, Lowary TL, Klassen JS, Evoy S, Szymanski CM.	Bacteriophage	10.4161/21597073.2014.960346	2014
Enzymology	Molecular epidemiology of Mycobacterium avium subsp. paratuberculosis: evidence for limited strain diversity, strain sharing, and identification of unique targets for diagnosis.	Motiwala AS, Strother M, Amonsin A, Byrum B, Naser SA, Stabel JR, Shulaw WP, Bannantine JP, Kapur V, Sreevatsan S.	J Clin Microbiol	10.1128/jcm.41.5.2015-2026.2003	2003
	Mechanisms of Antimicrobial Action of Cinnamon and Oregano Oils, Cinnamaldehyde, Carvacrol, 2,5-Dihydroxybenzaldehyde, and 2-Hydroxy-5-Methoxybenzaldehyde against Mycobacterium avium subsp. paratuberculosis (Map).	Nowotarska SW, Nowotarski K, Grant IR, Elliott CT, Friedman M, Situ C.	Foods	10.3390/foods6090072	2017
Enzymology	Maximizing capture efficiency and specificity of magnetic separation for Mycobacterium avium subsp. paratuberculosis cells.	Foddai A, Elliott CT, Grant IR.	Appl Environ Microbiol	10.1128/aem.01432-10	2010
Stress	Rapid assessment of the viability of Mycobacterium avium subsp. paratuberculosis cells after heat treatment, using an optimized phage amplification assay.	Foddai A, Elliott CT, Grant IR.	Appl Environ Microbiol	10.1128/aem.02625-09	2010
Stress	Efficacy of various pasteurization time-temperature conditions in combination with homogenization on inactivation of Mycobacterium avium subsp. paratuberculosis in milk.	Grant IR, Williams AG, Rowe MT, Muir DD.	Appl Environ Microbiol	10.1128/aem.71.6.2853-2861.2005	2005
Enzymology	Optimization of a phage amplification assay to permit accurate enumeration of viable Mycobacterium avium subsp. paratuberculosis cells.	Foddai A, Elliott CT, Grant IR.	Appl Environ Microbiol	10.1128/aem.00294-09	2009
	UV light inactivation of Mycobacterium avium subsp. paratuberculosis in milk as assessed by FASTPlaqueTB phage assay and culture.	Altic LC, Rowe MT, Grant IR.	Appl Environ Microbiol	10.1128/aem.00057-07	2007
Enzymology	New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis.	Herthnek D, Bolske G.	BMC Microbiol	10.1186/1471-2180-6-87	2006
	The efficacy and safety of high-pressure processing of food.	EFSA Panel on Biological Hazards (BIOHAZ Panel), Koutsoumanis K, Alvarez-Ordonez A, Bolton D, Bover-Cid S, Chemaly M, Davies R, De Cesare A, Herman L, Hilbert F, Lindqvist R, Nauta M, Peixe L, Ru G, Simmons M, Skandamis P, Suffredini E, Castle L, Crotta M, Grob K, Milana MR, Petersen A, Roig Sagues AX, Vinagre Silva F, Barthelemy E, Christodoulidou A, Messens W, Allende A.	EFSA J	10.2903/j.efsa.2022.7128	2022
Enzymology	Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation.	Grant IR, Ball HJ, Rowe MT.	Appl Environ Microbiol	10.1128/aem.64.9.3153-3158.1998	1998
Phylogeny	Molecular characterization of pigmented and nonpigmented isolates of Mycobacterium avium subsp. paratuberculosis.	Stevenson K, Hughes VM, de Juan L, Inglis NF, Wright F, Sharp JM.	J Clin Microbiol	10.1128/jcm.40.5.1798-1804.2002	2002
Enzymology	Inactivation of Mycobacterium paratuberculosis in cows' milk at pasteurization temperatures.	Grant IR, Ball HJ, Neill SD, Rowe MT.	Appl Environ Microbiol	10.1128/aem.62.2.631-636.1996	1996
	Rapid identification of mycobacteria from AIDS patients by capillary electrophoretic profiling of amplified SOD gene.	Bull TJ, Shanson DC, Archard LC.	Clin Mol Pathol	10.1136/mp.48.3.m124	1995
Enzymology	Incidence of Mycobacterium paratuberculosis in bulk raw and commercially pasteurized cows' milk from approved dairy processing establishments in the United Kingdom.	Grant IR, Ball HJ, Rowe MT.	Appl Environ Microbiol	10.1128/aem.68.5.2428-2435.2002	2002
Enzymology	Antibacterial activities of naturally occurring compounds against Mycobacterium avium subsp. paratuberculosis.	Wong SY, Grant IR, Friedman M, Elliott CT, Situ C	Appl Environ Microbiol	10.1128/AEM.00981-08	2008
Cultivation	Persistence of Mycobacterium paratuberculosis during manufacture and ripening of cheddar cheese.	Donaghy JA, Totton NL, Rowe MT	Appl Environ Microbiol	10.1128/AEM.70.8.4899-4905.2004	2004
Phylogeny	Effect of chemical decontamination and refrigerated storage on the isolation of Mycobacterium avium subsp. paratuberculosis from heat-treated milk.	Grant IR, Rowe MT	Lett Appl Microbiol	10.1111/j.1472-765x.2004.01498.x	2004
Phylogeny	Iodixanol development of a laboratory-scale technique to monitor the persistence of Mycobacterium avium subsp. paratuberculosis in Cheddar cheese.	Donaghy JA, Totton NL, Rowe MT	ScientificWorldJournal	10.1100/tsw.2003.109	2003
Pathogenicity	Bactericidal effect of chlorine on Mycobacterium paratuberculosis in drinking water.	Whan LB, Grant IR, Ball HJ, Scott R, Rowe MT	Lett Appl Microbiol	10.1046/j.1472-765x.2001.00987.x	2001
Stress	Effect of higher pasteurization temperatures, and longer holding times at 72 degrees C, on the inactivation of Mycobacterium paratuberculosis in milk.	Grant IR, Ball HJ, Rowe MT	Lett Appl Microbiol	10.1046/j.1365-2672.1999.00557.x	1999
Pathogenicity	Interaction between Mycobacterium avium subsp. paratuberculosis and environmental protozoa.	Whan L, Grant IR, Rowe MT	BMC Microbiol	10.1186/1471-2180-6-63	2006

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34445	Collection of Institut Pasteur ; Curators of the CIP; CIP 103964
#68382	Automatically annotated from API zym .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium avium subsp. paratuberculosis (CIP 103964, ATCC 19851, NCTC 8578)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

BacDive

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