Methanosarcina mazei (DSM 10132, SarPi)

Name: Methanosarcina mazei (DSM 10132, SarPi)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7107

Type strain

Strain Designation SarPi

Culture col. no. DSM 10132 SarPi

NCBI tax ID(s) 2209

Special Collections DSMZ

History <- J.-L. Garcia; SarPi <- C. Joulian {1995}

Links

Methanosarcina mazei SarPi is an anaerobe, mesophilic prokaryote that was isolated from ricefield soil.

anaerobe mesophilic genome sequence

Name and taxonomic classification

SI-ID 48759

DSM 10132

@ref 20215

Last LPSN update 2026-02-09 11:19:23

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina mazei

Full scientific name Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

Synonyms (4)

Methanosarcina frisia
Methanococcus frisius
Methanosarcina mazeii
Methanococcus mazei

BacDive ID	Other strains from **Methanosarcina mazei** (13)	Type strain
7096	M. mazei S-6, DSM 2053, ATCC 43572, OCM 26, DSM 108209 (type strain)
7097	M. mazei Z-558, DSM 2244, OCM 92
7098	M. mazei MC3, DSM 2907, OCM 192
7099	M. mazei C16, DSM 3318, ATCC 43340, OCM 98
7100	M. mazei G1, DSM 3338, OCM 87
7101	M. mazei Gö1, DSM 3647, ATCC BAA-159, JCM 11833, ...
7102	M. mazei DSM 4556, ATCC 43573, OCM 34
7103	M. mazei T18, DSM 6300
7104	M. mazei Equi, DSM 7058, OCM 61
7105	M. mazei Gö1, DSM 7222
7106	M. mazei TMA, DSM 9195, JCM 9314, NBRC 101201
24166	M. mazei O1M9701, DSM 27785, OCM 748
164152	M. mazei JCM 31898, VKM B-2370

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	99.4

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3812	METHANOSARCINA MEDIUM (DSMZ Medium 120)		Medium recipe at MediaDive	Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
3812	METHANOBACTERIUM II MEDIUM (N2/CO2) (DSMZ Medium 825a)		Medium recipe at MediaDive	Name: METHANOBACTERIUM II MEDIUM (N2/CO2) (DSMZ Medium 825a) Composition: Methanol 7.7571 g/l NaHCO3 3.91773 g/l NH4Cl 0.979432 g/l Yeast extract 0.979432 g/l L-Cysteine HCl x H2O 0.489716 g/l Na2S x 9 H2O 0.489716 g/l Na-acetate 0.489716 g/l K2HPO4 0.29383 g/l KH2PO4 0.29383 g/l MgCl2 x 6 H2O 0.195886 g/l KCl 0.0979432 g/l MgSO4 x 7 H2O 0.029383 g/l Nitrilotriacetic acid 0.0146915 g/l NaCl 0.00979432 g/l MnSO4 x H2O 0.00489716 g/l ZnSO4 x 7 H2O 0.00176298 g/l CoSO4 x 7 H2O 0.00176298 g/l CaCl2 x 2 H2O 0.000979432 g/l FeSO4 x 7 H2O 0.000979432 g/l Sodium resazurin 0.000489716 g/l NiCl2 x 6 H2O 0.00029383 g/l AlK(SO4)2 x 12 H2O 0.000195886 g/l H3BO3 9.79432e-05 g/l CuSO4 x 5 H2O 9.79432e-05 g/l Na2MoO4 x 2 H2O 9.79432e-05 g/l Pyridoxine hydrochloride 9.79432e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Biotin 1.95886e-05 g/l Folic acid 1.95886e-05 g/l Na2WO4 x 2 H2O 3.91773e-06 g/l Na2SeO3 x 5 H2O 2.9383e-06 g/l Vitamin B12 9.79432e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3812	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

3812

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		96.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
3812	ricefield soil	Philippines	PHL	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3812	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM97018v1 assembly for Methanosarcina mazei SarPi	complete	GCA_000970185	1434115.4	2648501772	1434115	98.51

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanosarcina mazei SarPi
NCBI: GCA_000970185

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	96.10	no
125439	motility	BacteriaNetⓘ	no	80.00	no
125439	gram_stain	BacteriaNetⓘ	negative	99.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	75.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanosarcina mazei SarPi SarPi
NCBI: GCA_000970185.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	64.80	no
125438	anaerobic	anaerobicⓘ	yes	86.28	no
125438	aerobic	aerobicⓘ	no	89.86	no
125438	spore-forming	spore-formingⓘ	no	81.30	no
125438	thermophilic	thermophileⓘ	no	86.07	yes
125438	flagellated	motile2+ⓘ	no	84.21	no

Literature

References

#3812	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10132
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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