Methanosarcina mazei (DSM 7222, Gö1)

Name: Methanosarcina mazei (DSM 7222, Gö1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7105

Type strain

Strain Designation Gö1

Culture col. no. DSM 7222 Gö1

NCBI tax ID(s) 2209

Special Collections DSMZ

History <- R. I. L. Eggen, Wageningen University, Laboratory of Microbiology, Netherlands (Methanosarcina frisia) <- M. Blaut, Universität Göttingen, Germany <- Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH <- F. Widdel, Universität Göttingen, Germany; Gö1 {1978}

Links

Methanosarcina mazei Gö1 is an anaerobe, mesophilic prokaryote that was isolated from anaerobic sewage digester, derived from strain DSM 3647.

anaerobe mesophilic

Name and taxonomic classification

SI-ID 48388

DSM 7222

@ref 20215

Last LPSN update 2026-02-09 11:19:23

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina mazei

Full scientific name Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

Synonyms (4)

Methanosarcina frisia
Methanococcus frisius
Methanosarcina mazeii
Methanococcus mazei

BacDive ID	Other strains from **Methanosarcina mazei** (13)	Type strain
7096	M. mazei S-6, DSM 2053, ATCC 43572, OCM 26, DSM 108209 (type strain)
7097	M. mazei Z-558, DSM 2244, OCM 92
7098	M. mazei MC3, DSM 2907, OCM 192
7099	M. mazei C16, DSM 3318, ATCC 43340, OCM 98
7100	M. mazei G1, DSM 3338, OCM 87
7101	M. mazei Gö1, DSM 3647, ATCC BAA-159, JCM 11833, ...
7102	M. mazei DSM 4556, ATCC 43573, OCM 34
7103	M. mazei T18, DSM 6300
7104	M. mazei Equi, DSM 7058, OCM 61
7106	M. mazei TMA, DSM 9195, JCM 9314, NBRC 101201
7107	M. mazei SarPi, DSM 10132
24166	M. mazei O1M9701, DSM 27785, OCM 748
164152	M. mazei JCM 31898, VKM B-2370

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3058	METHANOSARCINA MAZEI (GÖ1) MEDIUM (DSMZ Medium 120c)		Medium recipe at MediaDive	Name: METHANOSARCINA MAZEI (GOE1) MEDIUM (DSMZ Medium 120c) Composition: Sucrose 17.0319 g/l Na-acetate 9.96016 g/l NaCl 2.24104 g/l Yeast extract 1.99203 g/l Casitone 1.99203 g/l NaHCO3 1.99203 g/l NH4Cl 0.498008 g/l MgSO4 x 7 H2O 0.498008 g/l K2HPO4 0.348606 g/l Na2S x 9 H2O 0.298805 g/l L-Cysteine HCl x H2O 0.298805 g/l CaCl2 x 2 H2O 0.249004 g/l KH2PO4 0.229084 g/l HCl 0.00249004 g/l FeSO4 x 7 H2O 0.00199203 g/l FeCl2 x 4 H2O 0.00149402 g/l Sodium resazurin 0.000498008 g/l CoCl2 x 6 H2O 0.000189243 g/l Pyridoxine hydrochloride 9.96016e-05 g/l MnCl2 x 4 H2O 9.96016e-05 g/l ZnCl2 6.97211e-05 g/l (DL)-alpha-Lipoic acid 4.98008e-05 g/l Thiamine HCl 4.98008e-05 g/l p-Aminobenzoic acid 4.98008e-05 g/l Calcium D-(+)-pantothenate 4.98008e-05 g/l Nicotinic acid 4.98008e-05 g/l Riboflavin 4.98008e-05 g/l Na2MoO4 x 2 H2O 3.58566e-05 g/l NiCl2 x 6 H2O 2.39044e-05 g/l Biotin 1.99203e-05 g/l Folic acid 1.99203e-05 g/l H3BO3 5.9761e-06 g/l CuCl2 x 2 H2O 1.99203e-06 g/l Vitamin B12 9.96016e-07 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3058	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

3058

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3058	anaerobic sewage digester, derived from strain DSM 3647	Göttingen	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3058	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Methanosarcina Spherical Virus, a Novel Archaeal Lytic Virus Targeting Methanosarcina Strains.	Weidenbach K, Nickel L, Neve H, Alkhnbashi OS, Kunzel S, Kupczok A, Bauersachs T, Cassidy L, Tholey A, Backofen R, Schmitz RA.	J Virol	10.1128/jvi.00955-17	2017
Metabolism	A novel inducible protein production system and neomycin resistance as selection marker for Methanosarcina mazei.	Mondorf S, Deppenmeier U, Welte C.	Archaea	10.1155/2012/973743	2012
Metabolism	Function of Ech hydrogenase in ferredoxin-dependent, membrane-bound electron transport in Methanosarcina mazei.	Welte C, Kallnik V, Grapp M, Bender G, Ragsdale S, Deppenmeier U.	J Bacteriol	10.1128/jb.01307-09	2010
Enzymology	Transcriptional profiling of methyltransferase genes during growth of Methanosarcina mazei on trimethylamine.	Kratzer C, Carini P, Hovey R, Deppenmeier U.	J Bacteriol	10.1128/jb.00420-09	2009
Metabolism	The Methanothermobacter thermautotrophicus ExoIII homologue Mth212 is a DNA uridine endonuclease.	Georg J, Schomacher L, Chong JP, Majernik AI, Raabe M, Urlaub H, Muller S, Ciirdaeva E, Kramer W, Fritz HJ.	Nucleic Acids Res	10.1093/nar/gkl604	2006

References

#3058	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7222
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanosarcina mazei (DSM 7222, Gö1)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

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