Strain identifier
BacDive ID: 7107
Type strain:
Species: Methanosarcina mazei
Strain Designation: SarPi
Strain history: <- J.-L. Garcia; SarPi <- C. Joulian {1995}
NCBI tax ID(s): 2209 (species)
General
@ref: 3812
BacDive-ID: 7107
DSM-Number: 10132
keywords: genome sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina mazei SarPi is an anaerobe, mesophilic archaeon that was isolated from ricefield soil.
NCBI tax id
- NCBI tax id: 2209
- Matching level: species
strain history
- @ref: 3812
- history: <- J.-L. Garcia; SarPi <- C. Joulian {1995}
doi: 10.13145/bacdive7107.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina mazei
- full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
synonyms
@ref synonym 20215 Methanosarcina frisia 20215 Methanococcus frisius 20215 Methanosarcina mazeii 20215 Methanococcus mazei
@ref: 3812
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina mazei
full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984
strain designation: SarPi
type strain: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3812 | METHANOSARCINA MEDIUM (DSMZ Medium 120) | yes | https://mediadive.dsmz.de/medium/120 | Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water |
3812 | METHANOBACTERIUM II MEDIUM (N2/CO2) (DSMZ Medium 825a) | yes | https://mediadive.dsmz.de/medium/825a | Name: METHANOBACTERIUM II MEDIUM (N2/CO2) (DSMZ Medium 825a) Composition: Methanol 7.7571 g/l NaHCO3 3.91773 g/l NH4Cl 0.979432 g/l Yeast extract 0.979432 g/l L-Cysteine HCl x H2O 0.489716 g/l Na2S x 9 H2O 0.489716 g/l Na-acetate 0.489716 g/l K2HPO4 0.29383 g/l KH2PO4 0.29383 g/l MgCl2 x 6 H2O 0.195886 g/l KCl 0.0979432 g/l MgSO4 x 7 H2O 0.029383 g/l Nitrilotriacetic acid 0.0146915 g/l NaCl 0.00979432 g/l MnSO4 x H2O 0.00489716 g/l ZnSO4 x 7 H2O 0.00176298 g/l CoSO4 x 7 H2O 0.00176298 g/l CaCl2 x 2 H2O 0.000979432 g/l FeSO4 x 7 H2O 0.000979432 g/l Sodium resazurin 0.000489716 g/l NiCl2 x 6 H2O 0.00029383 g/l AlK(SO4)2 x 12 H2O 0.000195886 g/l H3BO3 9.79432e-05 g/l CuSO4 x 5 H2O 9.79432e-05 g/l Na2MoO4 x 2 H2O 9.79432e-05 g/l Pyridoxine hydrochloride 9.79432e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Biotin 1.95886e-05 g/l Folic acid 1.95886e-05 g/l Na2WO4 x 2 H2O 3.91773e-06 g/l Na2SeO3 x 5 H2O 2.9383e-06 g/l Vitamin B12 9.79432e-07 g/l Distilled water |
culture temp
- @ref: 3812
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 3812
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 3812
- sample type: ricefield soil
- country: Philippines
- origin.country: PHL
- continent: Asia
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Agriculture | #Field |
#Environmental | #Terrestrial | #Soil |
#Host | #Plants | #Herbaceous plants (Grass,Crops) |
Safety information
risk assessment
- @ref: 3812
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Methanosarcina mazei SarPi | GCA_000970185 | complete | ncbi | 1434115 |
66792 | Methanosarcina mazei SarPi | 2648501772 | complete | img | 1434115 |
66792 | Methanosarcina mazei SarPi | 1434115.4 | complete | patric | 1434115 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 90.943 | no |
flagellated | no | 96.985 | no |
gram-positive | no | 94.916 | no |
anaerobic | yes | 97.147 | yes |
aerobic | no | 94.095 | yes |
halophile | no | 89.504 | no |
spore-forming | no | 94.398 | no |
thermophile | no | 97.4 | yes |
glucose-util | yes | 70.229 | no |
glucose-ferment | no | 70.703 | no |
External links
@ref: 3812
culture collection no.: DSM 10132
straininfo link
- @ref: 76562
- straininfo: 48759
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
3812 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10132) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10132 | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
76562 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48759.1 | StrainInfo: A central database for resolving microbial strain identifiers |