Strain identifier

BacDive ID: 7107

Type strain: No

Species: Methanosarcina mazei

Strain Designation: SarPi

Strain history: <- J.-L. Garcia; SarPi <- C. Joulian {1995}

NCBI tax ID(s): 2209 (species)

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General

@ref: 3812

BacDive-ID: 7107

DSM-Number: 10132

keywords: genome sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina mazei SarPi is an anaerobe, mesophilic archaeon that was isolated from ricefield soil.

NCBI tax id

  • NCBI tax id: 2209
  • Matching level: species

strain history

  • @ref: 3812
  • history: <- J.-L. Garcia; SarPi <- C. Joulian {1995}

doi: 10.13145/bacdive7107.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina mazei
  • full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
  • synonyms

    @refsynonym
    20215Methanosarcina frisia
    20215Methanococcus frisius
    20215Methanosarcina mazeii
    20215Methanococcus mazei

@ref: 3812

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: SarPi

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3812METHANOSARCINA MEDIUM (DSMZ Medium 120)yeshttps://mediadive.dsmz.de/medium/120Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
3812METHANOBACTERIUM II MEDIUM (N2/CO2) (DSMZ Medium 825a)yeshttps://mediadive.dsmz.de/medium/825aName: METHANOBACTERIUM II MEDIUM (N2/CO2) (DSMZ Medium 825a) Composition: Methanol 7.7571 g/l NaHCO3 3.91773 g/l NH4Cl 0.979432 g/l Yeast extract 0.979432 g/l L-Cysteine HCl x H2O 0.489716 g/l Na2S x 9 H2O 0.489716 g/l Na-acetate 0.489716 g/l K2HPO4 0.29383 g/l KH2PO4 0.29383 g/l MgCl2 x 6 H2O 0.195886 g/l KCl 0.0979432 g/l MgSO4 x 7 H2O 0.029383 g/l Nitrilotriacetic acid 0.0146915 g/l NaCl 0.00979432 g/l MnSO4 x H2O 0.00489716 g/l ZnSO4 x 7 H2O 0.00176298 g/l CoSO4 x 7 H2O 0.00176298 g/l CaCl2 x 2 H2O 0.000979432 g/l FeSO4 x 7 H2O 0.000979432 g/l Sodium resazurin 0.000489716 g/l NiCl2 x 6 H2O 0.00029383 g/l AlK(SO4)2 x 12 H2O 0.000195886 g/l H3BO3 9.79432e-05 g/l CuSO4 x 5 H2O 9.79432e-05 g/l Na2MoO4 x 2 H2O 9.79432e-05 g/l Pyridoxine hydrochloride 9.79432e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Biotin 1.95886e-05 g/l Folic acid 1.95886e-05 g/l Na2WO4 x 2 H2O 3.91773e-06 g/l Na2SeO3 x 5 H2O 2.9383e-06 g/l Vitamin B12 9.79432e-07 g/l Distilled water

culture temp

  • @ref: 3812
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 3812
  • oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

  • @ref: 3812
  • sample type: ricefield soil
  • country: Philippines
  • origin.country: PHL
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

Safety information

risk assessment

  • @ref: 3812
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Methanosarcina mazei SarPiGCA_000970185completencbi1434115
66792Methanosarcina mazei SarPi2648501772completeimg1434115
66792Methanosarcina mazei SarPi1434115.4completepatric1434115

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno90.943no
flagellatedno96.985no
gram-positiveno94.916no
anaerobicyes97.147yes
aerobicno94.095yes
halophileno89.504no
spore-formingno94.398no
thermophileno97.4yes
glucose-utilyes70.229no
glucose-fermentno70.703no

External links

@ref: 3812

culture collection no.: DSM 10132

straininfo link

  • @ref: 76562
  • straininfo: 48759

Reference

@idauthorscataloguedoi/urltitle
3812Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10132)https://www.dsmz.de/collection/catalogue/details/culture/DSM-10132
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76562Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID48759.1StrainInfo: A central database for resolving microbial strain identifiers