Methanosarcina mazei (DSM 2053, ATCC 43572, OCM 26)

Name: Methanosarcina mazei (DSM 2053, ATCC 43572, OCM 26)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7096

Type strain

Strain Designation S-6

Culture col. no. DSM 2053 ATCC 43572 OCM 26 S-6 DSM 108209

NCBI tax ID(s) 2209

Special Collections DSMZ

Links

Methanosarcina mazei S-6 is an anaerobe, mesophilic prokaryote that was isolated from sewage sludge plant.

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 41776

DSM 108209,DSM 2053,ATCC 43572,VKM B-1636

@ref 20215

Last LPSN update 2025-12-16 09:48:29

Domain Methanobacteriati

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina mazei

Full scientific name Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

Synonyms (4)

Methanosarcina frisia
Methanococcus frisius
Methanosarcina mazeii
Methanococcus mazei

BacDive ID	Other strains from **Methanosarcina mazei** (13)	Type strain
7097	M. mazei Z-558, DSM 2244, OCM 92
7098	M. mazei MC3, DSM 2907, OCM 192
7099	M. mazei C16, DSM 3318, ATCC 43340, OCM 98
7100	M. mazei G1, DSM 3338, OCM 87
7101	M. mazei Gö1, DSM 3647, ATCC BAA-159, JCM 11833, ...
7102	M. mazei DSM 4556, ATCC 43573, OCM 34
7103	M. mazei T18, DSM 6300
7104	M. mazei Equi, DSM 7058, OCM 61
7105	M. mazei Gö1, DSM 7222
7106	M. mazei TMA, DSM 9195, JCM 9314, NBRC 101201
7107	M. mazei SarPi, DSM 10132
24166	M. mazei O1M9701, DSM 27785, OCM 748
164152	M. mazei JCM 31898, VKM B-2370

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	98.8

Multimedia

@ref:	690
multimedia content:	DSM_2053.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_2053.jpg
caption:	Medium 120, 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
690	METHANOSARCINA MEDIUM (DSMZ Medium 120)		Medium recipe at MediaDive	Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
690	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

690

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		95.6

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Plant (Factory)
#Engineered	#Waste	#Sewage sludge

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
690	sewage sludge plant	California, El Segundo	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AJ012095 (>99% sequence identity) for Methanosarcina from Microbeatlas

Aquatic Samples	3372
Soil Samples	2215
Animal Samples	684
Plant Samples	931
Total Samples	7202

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
690	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM97020v1 assembly for Methanosarcina mazei S-6	complete	GCA_000970205	213585.10	2627853861	213585	98.24

Genome browser: GCA_000970205

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
690	Methanosarcina mazei 16S rRNA gene, strain DSM 2053 (T)	AJ012095	1333		2209
124043	Methanosarcina mazei gene for 16S ribosomal RNA, partial sequence, strain: DSM 2053.	AB973358	1406		2209

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanosarcina mazei S-6
NCBI: GCA_000970205

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	95.60	no
125439	motility	BacteriaNetⓘ	no	79.90	no
125439	gram_stain	BacteriaNetⓘ	negative	98.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	83.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanosarcina mazei S-6 S-6
NCBI: GCA_000970205.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	66.17	no
125438	anaerobic	anaerobicⓘ	yes	84.80	yes
125438	aerobic	aerobicⓘ	no	91.21	no
125438	spore-forming	spore-formingⓘ	no	81.46	no
125438	thermophilic	thermophileⓘ	no	85.20	no
125438	flagellated	motile2+ⓘ	no	83.71	no

Literature

Title	Authors	Journal	DOI	Year
Archaeosomes: New Generation of Liposomes Based on Archaeal Lipids for Drug Delivery and Biomedical Applications.	Santhosh PB, Genova J.	ACS Omega	10.1021/acsomega.2c06034	2023
Methanogenic Archaea Can Produce Methane in Deliquescence-Driven Mars Analog Environments.	Maus D, Heinz J, Schirmack J, Airo A, Kounaves SP, Wagner D, Schulze-Makuch D.	Sci Rep	10.1038/s41598-019-56267-4	2020
Response of Methanogen Communities to the Elevation of Cathode Potentials in Bioelectrochemical Reactors Amended with Magnetite.	Gao K, Wang X, Huang J, Xia X, Lu Y.	Appl Environ Microbiol	10.1128/aem.01488-21	2021
Gut Colonization with Methanogenic Archaea Lowers Plasma Trimethylamine N-oxide Concentrations in Apolipoprotein e-/- Mice.	Ramezani A, Nolin TD, Barrows IR, Serrano MG, Buck GA, Regunathan-Shenk R, West RE, Latham PS, Amdur R, Raj DS.	Sci Rep	10.1038/s41598-018-33018-5	2018
Methane production in aerated marshland and model soils: effects of microflora and soil texture	Wagner D, Pfeiffer EM, Bock E.	Soil Biol Biochem	10.1016/s0038-0717(99)00011-5	1999
Diversity and Niche of Archaea in Bioremediation.	Krzmarzick MJ, Taylor DK, Fu X, McCutchan AL.	Archaea	10.1155/2018/3194108	2018
Metabolic flexibility as a major predictor of spatial distribution in microbial communities.	Carbonero F, Oakley BB, Purdy KJ.	PLoS One	10.1371/journal.pone.0085105	2014
A genetic system for Archaea of the genus Methanosarcina: liposome-mediated transformation and construction of shuttle vectors.	Metcalf WW, Zhang JK, Apolinario E, Sowers KR, Wolfe RS.	Proc Natl Acad Sci U S A	10.1073/pnas.94.6.2626	1997
The purine-utilizing bacterium Clostridium acidurici 9a: a genome-guided metabolic reconsideration.	Hartwich K, Poehlein A, Daniel R.	PLoS One	10.1371/journal.pone.0051662	2012
Archaeosomes varying in lipid composition differ in receptor-mediated endocytosis and differentially adjuvant immune responses to entrapped antigen.	Sprott GD, Sad S, Fleming LP, Dicaire CJ, Patel GB, Krishnan L.	Archaea	10.1155/2003/569283	2003
Hydroxydiether Lipid Structures in Methanosarcina spp. and Methanococcus voltae.	Sprott GD, Dicaire CJ, Choquet CG, Patel GB, Ekiel I.	Appl Environ Microbiol	10.1128/aem.59.3.912-914.1993	1993
Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei.	Peck MW.	Appl Environ Microbiol	10.1128/aem.55.4.940-945.1989	1989
Inhibition of methanogenesis by methyl fluoride: studies of pure and defined mixed cultures of anaerobic bacteria and archaea.	Janssen PH, Frenzel P.	Appl Environ Microbiol	10.1128/aem.63.11.4552-4557.1997	1997
Spontaneous Disaggregation of Methanosarcina mazei S-6 and Its Use in the Development of Genetic Techniques for Methanosarcina spp.	Harris JE.	Appl Environ Microbiol	10.1128/aem.53.10.2500-2504.1987	1987
Enzyme-Linked Immunosorbent Assays for the Specific and Sensitive Quantification of Methanosarcina mazei and Methanobacterium bryantii.	Kemp HA, Archer DB, Morgan MR.	Appl Environ Microbiol	10.1128/aem.54.4.1003-1008.1988	1988
Methanogenesis from ethanol by defined mixed continuous cultures.	Tatton MJ, Archer DB, Powell GE, Parker ML.	Appl Environ Microbiol	10.1128/aem.55.2.440-445.1989	1989
Disaggregation of Methanosarcina spp. and Growth as Single Cells at Elevated Osmolarity.	Sowers KR, Boone JE, Gunsalus RP.	Appl Environ Microbiol	10.1128/aem.59.11.3832-3839.1993	1993
Formation of unilamellar liposomes from total polar lipid extracts of methanogens.	Choquet CG, Patel GB, Beveridge TJ, Sprott GD.	Appl Environ Microbiol	10.1128/aem.58.9.2894-2900.1992	1992
Immobilization patterns and dynamics of acetate-utilizing methanogens immobilized in sterile granular sludge in upflow anaerobic sludge blanket reactors.	Schmidt JE, Ahring BK.	Appl Environ Microbiol	10.1128/aem.65.3.1050-1054.1999	1999
Plasmid DNA from the acetotrophic methanogen Methanosarcina acetivorans.	Sowers KR, Gunsalus RP.	J Bacteriol	10.1128/jb.170.10.4979-4982.1988	1988
Archaeosome vaccine adjuvants induce strong humoral, cell-mediated, and memory responses: comparison to conventional liposomes and alum.	Krishnan L, Dicaire CJ, Patel GB, Sprott GD.	Infect Immun	10.1128/iai.68.1.54-63.2000	2000
ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea.	Verhees CH, Tuininga JE, Kengen SW, Stams AJ, van der Oost J, de Vos WM.	J Bacteriol	10.1128/jb.183.24.7145-7153.2001	2001
Potassium extrusion by the moderately halophilic and alkaliphilic methanogen methanolobus taylorii GS-16 and homeostasis of cytosolic pH.	Ni S, Boone JE, Boone DR.	J Bacteriol	10.1128/jb.176.23.7274-7279.1994	1994
Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil.	Wagner D, Schirmack J, Ganzert L, Morozova D, Mangelsdorf K	Int J Syst Evol Microbiol	10.1099/ijs.0.046565-0	2013

References

#690	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2053
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanosarcina mazei (DSM 2053, ATCC 43572, OCM 26)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000970205

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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