Methanosarcina mazei DSM 4556 is an anaerobe, mesophilic prokaryote that was isolated from alkaline mud, oil exploration drilling site.
anaerobe mesophilic 16S sequence| @ref 20215 |
Last LPSN update
2025-12-16 09:48:29 |
| Domain Methanobacteriati |
| Phylum Methanobacteriota |
| Class Methanosarcinia |
| Order Methanosarcinales |
| Family Methanosarcinaceae |
| Genus Methanosarcina |
| Species Methanosarcina mazei |
| Full scientific name Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984 |
| Synonyms (4) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 1716 | METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) | Medium recipe at MediaDive  | Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a; with strain-specific modifications) Composition: Methanol 3.86719 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 1716 | positive | growth | 37 | mesophilic |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Industrial | #Oil reservoir | |
| #Environmental | #Terrestrial | #Mud (Sludge) | |
| #Condition | #Alkaline | - |
| @ref | Sample type | Geographic location | Country | Country ISO 3 Code | Continent | |
|---|---|---|---|---|---|---|
| 1716 | alkaline mud, oil exploration drilling site | Hula swamp area of Galilee | Israel | ISR | Asia |
Global distribution of 16S sequence AB065296 (>99% sequence identity) for Methanosarcina from Microbeatlas 
 Aquatic Samples |
3372 |
 Soil Samples |
2215 |
 Animal Samples |
684 |
 Plant Samples |
931 |
| Total Samples | 7202 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Methanosarcina mazei gene for 16S rRNA | AB065296 | 1439 |   | 2209 |  |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Methanosarcina Spherical Virus, a Novel Archaeal Lytic Virus Targeting Methanosarcina Strains. | Weidenbach K, Nickel L, Neve H, Alkhnbashi OS, Kunzel S, Kupczok A, Bauersachs T, Cassidy L, Tholey A, Backofen R, Schmitz RA. | J Virol | 10.1128/jvi.00955-17 | 2017 | | |
| Enzymology | Identification of the gene for disaggregatase from Methanosarcina mazei. | Osumi N, Kakehashi Y, Matsumoto S, Nagaoka K, Sakai J, Miyashita K, Kimura M, Asakawa S. | Archaea | 10.1155/2008/949458 | 2008 | |
| Genetics | A genetic system for Archaea of the genus Methanosarcina: liposome-mediated transformation and construction of shuttle vectors. | Metcalf WW, Zhang JK, Apolinario E, Sowers KR, Wolfe RS. | Proc Natl Acad Sci U S A | 10.1073/pnas.94.6.2626 | 1997 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive7102.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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