Methanosarcina mazei MC3 is an anaerobe, mesophilic prokaryote that was isolated from digester sludge.
anaerobe mesophilic| @ref 20215 |
Last LPSN update
2026-02-09 11:19:23 |
| Domain Archaea |
| Phylum Methanobacteriota |
| Class Methanosarcinia |
| Order Methanosarcinales |
| Family Methanosarcinaceae |
| Genus Methanosarcina |
| Species Methanosarcina mazei |
| Full scientific name Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984 |
| Synonyms (4) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 1240 | METHANOSARCINA THERMOPHILA (BCYT) MEDIUM (DSMZ Medium 318) | Medium recipe at MediaDive  | Name: METHANOSARCINA THERMOPHILA (BCYT) MEDIUM (DSMZ Medium 318) Composition: Methanol 7.7571 g/l KHCO3 1.95886 g/l NH4Cl 0.979432 g/l Yeast extract 0.489716 g/l Trypticase peptone 0.489716 g/l Na2S x 9 H2O 0.29383 g/l KH2PO4 0.29383 g/l L-Cysteine HCl x H2O 0.29383 g/l Nitrilotriacetic acid 0.125367 g/l MgCl2 x 6 H2O 0.0979432 g/l NaCl 0.00979432 g/l FeCl2 x 4 H2O 0.00979432 g/l CaCl2 x 2 H2O 0.000979432 g/l MnCl2 x 4 H2O 0.000979432 g/l NiCl2 x 6 H2O 0.000979432 g/l ZnCl2 0.000979432 g/l Sodium resazurin 0.000489716 g/l Na2WO4 x 2 H2O 0.000391773 g/l Na2MoO4 x 2 H2O 0.00029383 g/l CoCl2 x 6 H2O 0.00029383 g/l Na2SeO3 x 5 H2O 0.00029383 g/l CuCl2 0.000195886 g/l H3BO3 9.79432e-05 g/l Pyridoxine hydrochloride 9.79432e-05 g/l Calcium D-(+)-pantothenate 4.89716e-05 g/l (DL)-alpha-Lipoic acid 4.89716e-05 g/l p-Aminobenzoic acid 4.89716e-05 g/l Nicotinic acid 4.89716e-05 g/l Riboflavin 4.89716e-05 g/l Thiamine HCl 4.89716e-05 g/l Folic acid 1.95886e-05 g/l Biotin 1.95886e-05 g/l Vitamin B12 9.79432e-07 g/l Distilled water |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 1240 | positive | growth | 35 | mesophilic |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Metabolism | Evaluation and optimization of PCR primers for selective and quantitative detection of marine ANME subclusters involved in sulfate-dependent anaerobic methane oxidation. | Timmers PHA, Widjaja-Greefkes HCA, Plugge CM, Stams AJM. | Appl Microbiol Biotechnol | 10.1007/s00253-017-8338-x | 2017 |  |
| The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows. | Vaidya JD, van den Bogert B, Edwards JE, Boekhorst J, van Gastelen S, Saccenti E, Plugge CM, Smidt H. | Front Microbiol | 10.3389/fmicb.2018.00092 | 2018 | | |
| Contact angle measurement and cell hydrophobicity of granular sludge from upflow anaerobic sludge bed reactors. | Daffonchio D, Thaveesri J, Verstraete W. | Appl Environ Microbiol | 10.1128/aem.61.10.3676-3680.1995 | 1995 | | |
| Enzymology | Bacteriological composition and structure of granular sludge adapted to different substrates. | Grotenhuis JT, Smit M, Plugge CM, Xu YS, van Lammeren AA, Stams AJ, Zehnder AJ. | Appl Environ Microbiol | 10.1128/aem.57.7.1942-1949.1991 | 1991 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive7098.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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