Methanosarcina mazei (DSM 27785, OCM 748, O1M9701)

Name: Methanosarcina mazei (DSM 27785, OCM 748, O1M9701)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24166

Type strain

Strain Designation O1M9701

Culture col. no. DSM 27785 OCM 748 O1M9701

NCBI tax ID(s) 2209

Special Collections DSMZ

History <- M.-C. Lai, National Chung Hsing University, Taichung, Taiwan; O1M9701 <- M.-C. Lai; {1997}

Links

Methanosarcina mazei O1M9701 is an anaerobe, mesophilic prokaryote that was isolated from sediment of estuarine environment.

anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 398958

DSM 27785

@ref 20215

Last LPSN update 2026-02-09 11:23:54

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina mazei

Full scientific name Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

Synonyms (4)

Methanosarcina frisia
Methanococcus frisius
Methanosarcina mazeii
Methanococcus mazei

BacDive ID	Other strains from **Methanosarcina mazei** (13)	Type strain
7096	M. mazei S-6, DSM 2053, ATCC 43572, OCM 26, DSM 108209 (type strain)
7097	M. mazei Z-558, DSM 2244, OCM 92
7098	M. mazei MC3, DSM 2907, OCM 192
7099	M. mazei C16, DSM 3318, ATCC 43340, OCM 98
7100	M. mazei G1, DSM 3338, OCM 87
7101	M. mazei Gö1, DSM 3647, ATCC BAA-159, JCM 11833, ...
7102	M. mazei DSM 4556, ATCC 43573, OCM 34
7103	M. mazei T18, DSM 6300
7104	M. mazei Equi, DSM 7058, OCM 61
7105	M. mazei Gö1, DSM 7222
7106	M. mazei TMA, DSM 9195, JCM 9314, NBRC 101201
7107	M. mazei SarPi, DSM 10132
164152	M. mazei JCM 31898, VKM B-2370

Morphology

Colony morphology

21016

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21016	METHANOSARCINA MB MEDIUM (1 G/L NACL) (DSMZ Medium 924b)		Medium recipe at MediaDive	Name: METHANOSARCINA MB MEDIUM (1 g/l NaCl) (DSMZ Medium 924b; with strain-specific modifications) Composition: NaHCO3 3.95648 g/l Methanol 3.13353 g/l Yeast extract 1.97824 g/l Trypticase peptone 1.97824 g/l MgCl2 x 6 H2O 0.98912 g/l NH4Cl 0.98912 g/l KCl 0.49456 g/l K2HPO4 0.395648 g/l Na2S x 9 H2O 0.24728 g/l L-Cysteine HCl x H2O 0.24728 g/l MgSO4 x 7 H2O 0.0296736 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l Sodium resazurin 0.00049456 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l H3BO3 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Folic acid 1.97824e-05 g/l Biotin 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21016	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

21016

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Estuary
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
21016	sediment of estuarine environment	Eriln Shi	Taiwan, Province of China	TWN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AF411469 (>99% sequence identity) for Methanosarcina from Microbeatlas

Aquatic Samples	3372
Soil Samples	2215
Animal Samples	684
Plant Samples	931
Total Samples	7202

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21016	1	Risk group (German classification)

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21016	Methanosarcina mazei strain O1M9701 16S ribosomal RNA gene, partial sequence	AF411469	1271		2209

Genome-based predictions

Literature

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21016	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 27785
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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