Name: Methanosarcina mazei TMA
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7106

Type strain:

Species: Methanosarcina mazei

Strain Designation: TMA

Culture col. no.: DSM 9195, JCM 9314, NBRC 101201

Strain history: S. Asakawa TMA.

NCBI tax ID(s): 1293038 (strain), 2209 (species)

SI-ID 48592

DSM 9195, JCM 9314, NBRC 101201

Special Collections

DSMZ
JCM

Other strains from Methanosarcina mazei

Section

Methanosarcina mazei TMA is an anaerobe archaeon that was isolated from paddy field soil.

anaerobe
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanosarcinales*
	Family	*Methanosarcinaceae*
	Genus	*Methanosarcina*
	Species	**Methanosarcina mazei**
	Full Scientific Name (LPSN)	Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

	Synonym	Methanosarcina frisia
	Synonym	Methanococcus frisius
	Synonym	Methanosarcina mazeii
	Synonym	Methanococcus mazei

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3509]

Culture medium

METHANOSARCINA MEDIUM (DSMZ Medium 120)

[Ref.: #3509]

Culture medium growth

[Ref.: #3509]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3509]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #3509]			growth	37 °C
[Ref.: #67770]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3509]

Oxygen tolerance

anaerobe

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3509]	Sample type/isolated from	paddy field soil
[Ref.: #3509]	Geographic location (country and/or sea, region)	Fukuoka prefecture, Chikugo city
[Ref.: #3509]	Country	Japan
[Ref.: #3509]	Country ISO 3 Code	JPN
[Ref.: #3509]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Paddy field soil in Chikugo
[Ref.: #67770]	Geographic location (country and/or sea, region)	Fukuoka
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Condition	#Anoxic (anaerobic)	-
#Condition	#Humid	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB065295 (>99% sequence identity) for Methanosarcina from Microbeatlas

Aquatic Samples	3372
Soil Samples	2215
Animal Samples	684
Plant Samples	931
Total Samples	7202

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3509]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Methanosarcina mazei gene for 16S rRNA	AB065295	1439	GenBank ENA	2209 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanosarcina mazei TMA	GCA_019669945	complete	GenBank ENA	2209 tax ID	*
[Ref.: #66792]	Methanosarcina mazei JCM 9314	GCA_001315865	contig	GenBank ENA	1293038 tax ID	*
[Ref.: #66792]	Methanosarcina mazei JCM 9314	2681813026	draft	JGI IMG/M	1293038 tax ID	*
[Ref.: #66792]	Methanosarcina mazei TMA	GCA_025035885	chromosome	GenBank ENA	1434116 tax ID	*
[Ref.: #66792]	Methanosarcina mazei strain TMA	2209.222	complete	PATRIC	2209 tax ID	*
[Ref.: #66792]	Methanosarcina mazei TMA	1434116.4	complete	PATRIC	1434116 tax ID	*

[Ref.: #3509]

GC-content

42.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methanosarcina mazei TMA NCBI: GCA_019669945.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	67.199	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	yes	85.445	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	89.794	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	82.341	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	83.641	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	84.708	no

Availability in culture collections External links

[Ref.: #3509]

Culture collection no.

DSM 9195, JCM 9314, NBRC 101201

[Ref.: #76561]

SI-ID 48592

References

#3509

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 9195

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76561

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID48592.1 )

* These data were automatically processed and therefore are not curated

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