Methanosarcina mazei (DSM 3647, ATCC BAA-159, JCM 11833)

Name: Methanosarcina mazei (DSM 3647, ATCC BAA-159, JCM 11833)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7101

Type strain

Strain Designation Gö1

Culture col. no. DSM 3647 ATCC BAA-159 JCM 11833 OCM 88 Gö1

NCBI tax ID(s) 2209

Special Collections DSMZ JCM

Links

Methanosarcina mazei Gö1 is an anaerobe, mesophilic prokaryote that was isolated from anaerobic sewage digester.

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 44307

ATCC BAA-159,DSM 3647,JCM 11833

@ref 20215

Last LPSN update 2025-12-16 09:48:29

Domain Methanobacteriati

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina mazei

Full scientific name Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984

Synonyms (4)

Methanosarcina frisia
Methanococcus frisius
Methanosarcina mazeii
Methanococcus mazei

BacDive ID	Other strains from **Methanosarcina mazei** (13)	Type strain
7096	M. mazei S-6, DSM 2053, ATCC 43572, OCM 26, DSM 108209 (type strain)
7097	M. mazei Z-558, DSM 2244, OCM 92
7098	M. mazei MC3, DSM 2907, OCM 192
7099	M. mazei C16, DSM 3318, ATCC 43340, OCM 98
7100	M. mazei G1, DSM 3338, OCM 87
7102	M. mazei DSM 4556, ATCC 43573, OCM 34
7103	M. mazei T18, DSM 6300
7104	M. mazei Equi, DSM 7058, OCM 61
7105	M. mazei Gö1, DSM 7222
7106	M. mazei TMA, DSM 9195, JCM 9314, NBRC 101201
7107	M. mazei SarPi, DSM 10132
24166	M. mazei O1M9701, DSM 27785, OCM 748
164152	M. mazei JCM 31898, VKM B-2370

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1435	METHANOSARCINA MEDIUM (DSMZ Medium 120)		Medium recipe at MediaDive	Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
1435	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

1435

Oxygen toleranceanaerobe

Compound production

1435

CompoundATPase

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Waste	#Wastewater
#Condition	#Anoxic (anaerobic)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1435	anaerobic sewage digester	Göttingen	Germany	DEU	Europe
67770	Sewage plant	Göttingen	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1435	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM706v1 assembly for Methanosarcina mazei Go1	complete	GCA_000007065	192952.21	638154509	192952	98.78

Genome browser: GCA_000007065

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Methanosarcina mazei Go1 culture DSM:3647 16S ribosomal RNA gene, partial sequence	JQ346757	1268		192952

GC content

@ref	GC-content (mol%)	Method
1435	41	sequence analysis

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanosarcina mazei Go1
PATRIC: 192952.21

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	66.67	no
125438	anaerobic	anaerobicⓘ	yes	84.63	yes
125438	spore-forming	spore-formingⓘ	no	79.46	no
125438	aerobic	aerobicⓘ	no	91.21	yes
125438	thermophilic	thermophileⓘ	no	85.08	no
125438	flagellated	motile2+ⓘ	no	84.21	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Newly Established Genetic System for Functional Analysis of MetSV.	Gehlert FO, Weidenbach K, Baruske B, Hallack D, Repnik U, Schmitz RA.	Int J Mol Sci	10.3390/ijms241311163	2023
Pathogenicity	Dual-RNAseq Analysis Unravels Virus-Host Interactions of MetSV and Methanosarcina mazei.	Gehlert FO, Sauerwein T, Weidenbach K, Repnik U, Hallack D, Forstner KU, Schmitz RA.	Viruses	10.3390/v14112585	2022
	Methanosarcina Spherical Virus, a Novel Archaeal Lytic Virus Targeting Methanosarcina Strains.	Weidenbach K, Nickel L, Neve H, Alkhnbashi OS, Kunzel S, Kupczok A, Bauersachs T, Cassidy L, Tholey A, Backofen R, Schmitz RA.	J Virol	10.1128/jvi.00955-17	2017
Enzymology	Localization of Methyl-Coenzyme M reductase as metabolic marker for diverse methanogenic Archaea.	Wrede C, Walbaum U, Ducki A, Heieren I, Hoppert M.	Archaea	10.1155/2013/920241	2013
Metabolism	Archaeosine Modification of Archaeal tRNA: Role in Structural Stabilization.	Turner B, Burkhart BW, Weidenbach K, Ross R, Limbach PA, Schmitz RA, de Crecy-Lagard V, Stedman KM, Santangelo TJ, Iwata-Reuyl D.	J Bacteriol	10.1128/jb.00748-19	2020
Metabolism	Extracellular electron uptake in Methanosarcinales is independent of multiheme c-type cytochromes.	Yee MO, Rotaru AE.	Sci Rep	10.1038/s41598-019-57206-z	2020
	Biofilm formation of mucosa-associated methanoarchaeal strains.	Bang C, Ehlers C, Orell A, Prasse D, Spinner M, Gorb SN, Albers SV, Schmitz RA.	Front Microbiol	10.3389/fmicb.2014.00353	2014
Metabolism	Physiological and molecular characterizations of the interactions in two cellulose-to-methane cocultures.	Lu H, Ng SK, Jia Y, Cai M, Lee PKH.	Biotechnol Biofuels	10.1186/s13068-017-0719-y	2017
Pathogenicity	Effects of antimicrobial peptides on methanogenic archaea.	Bang C, Schilhabel A, Weidenbach K, Kopp A, Goldmann T, Gutsmann T, Schmitz RA.	Antimicrob Agents Chemother	10.1128/aac.00661-12	2012
	Effects of nitrogen and carbon sources on transcription of soluble methyltransferases in Methanosarcina mazei strain Go1.	Veit K, Ehlers C, Schmitz RA.	J Bacteriol	10.1128/jb.187.17.6147-6154.2005	2005
Metabolism	ATP-dependent H+ -pump activity in inverted vesicles of Methanosarcina mazei Gö1 and characterization of membrane ATPase.	Inatomi KI.	J Bacteriol	10.1128/jb.178.8.2424-2426.1996	1996
Enzymology	Energy conservation by the H2:heterodisulfide oxidoreductase from Methanosarcina mazei Gö1: identification of two proton-translocating segments.	Ide T, Baumer S, Deppenmeier U.	J Bacteriol	10.1128/jb.181.13.4076-4080.1999	1999
Enzymology	Identification and analysis of proton-translocating pyrophosphatases in the methanogenic archaeon Methansarcina mazei.	Baumer S, Lentes S, Gottschalk G, Deppenmeier U.	Archaea	10.1155/2002/371325	2002
Enzymology	The A1A0 ATPase from Methanosarcina mazei: cloning of the 5' end of the aha operon encoding the membrane domain and expression of the proteolipid in a membrane-bound form in Escherichia coli.	Ruppert C, Wimmers S, Lemker T, Muller V.	J Bacteriol	10.1128/jb.180.13.3448-3452.1998	1998
Metabolism	Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Gö1.	Abken HJ, Tietze M, Brodersen J, Baumer S, Beifuss U, Deppenmeier U.	J Bacteriol	10.1128/jb.180.8.2027-2032.1998	1998
Enzymology	Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation.	Kavran JM, Gundllapalli S, O'Donoghue P, Englert M, Soll D, Steitz TA.	Proc Natl Acad Sci U S A	10.1073/pnas.0704769104	2007
Metabolism	Electron paramagnetic resonance spectroscopic and electrochemical characterization of the partially purified N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanosarcina mazei Gö1.	Lu WP, Becher B, Gottschalk G, Ragsdale SW.	J Bacteriol	10.1128/jb.177.9.2245-2250.1995	1995
Metabolism	Delta mu Na+ drives the synthesis of ATP via an delta mu Na(+)-translocating F1F0-ATP synthase in membrane vesicles of the archaeon Methanosarcina mazei Gö1.	Becher B, Muller V.	J Bacteriol	10.1128/jb.176.9.2543-2550.1994	1994
	Effect of sulfate on low-temperature anaerobic digestion.	Madden P, Al-Raei AM, Enright AM, Chinalia FA, de Beer D, O'Flaherty V, Collins G.	Front Microbiol	10.3389/fmicb.2014.00376	2014
Metabolism	Lysine-2,3-aminomutase and beta-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of Nepsilon-acetyl-beta-lysine and growth at high salinity.	Pfluger K, Baumann S, Gottschalk G, Lin W, Santos H, Muller V.	Appl Environ Microbiol	10.1128/aem.69.10.6047-6055.2003	2003
Metabolism	Impact of a wastewater treatment plant on microbial community composition and function in a hyporheic zone of a eutrophic river.	Atashgahi S, Aydin R, Dimitrov MR, Sipkema D, Hamonts K, Lahti L, Maphosa F, Kruse T, Saccenti E, Springael D, Dejonghe W, Smidt H.	Sci Rep	10.1038/srep17284	2015

References

#1435	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3647
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanosarcina mazei (DSM 3647, ATCC BAA-159, JCM 11833)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000007065

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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