Strain identifier

BacDive ID: 7101

Type strain: No

Species: Methanosarcina mazei

Strain Designation: Gö1

Strain history: DSM 3647 <-- F. Widdel Gö1.

NCBI tax ID(s): 2209 (species)

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General

@ref: 1435

BacDive-ID: 7101

DSM-Number: 3647

keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic

description: Methanosarcina mazei Gö1 is an anaerobe, mesophilic archaeon that was isolated from anaerobic sewage digester.

NCBI tax id

  • NCBI tax id: 2209
  • Matching level: species

strain history

@refhistory
1435<- F. Widdel, Gö1 (Methanosarcina sp.)
67770DSM 3647 <-- F. Widdel Gö1.

doi: 10.13145/bacdive7101.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Methanomicrobia
  • order: Methanosarcinales
  • family: Methanosarcinaceae
  • genus: Methanosarcina
  • species: Methanosarcina mazei
  • full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
  • synonyms

    @refsynonym
    20215Methanosarcina frisia
    20215Methanococcus frisius
    20215Methanosarcina mazeii
    20215Methanococcus mazei

@ref: 1435

domain: Archaea

phylum: Euryarchaeota

class: Methanomicrobia

order: Methanosarcinales

family: Methanosarcinaceae

genus: Methanosarcina

species: Methanosarcina mazei

full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984

strain designation: Gö1

type strain: no

Culture and growth conditions

culture medium

  • @ref: 1435
  • name: METHANOSARCINA MEDIUM (DSMZ Medium 120)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/120
  • composition: Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

culture temp

@refgrowthtypetemperaturerange
1435positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 1435
  • oxygen tolerance: anaerobe

compound production

  • @ref: 1435
  • compound: ATPase

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
1435anaerobic sewage digesterGöttingenGermanyDEUEurope
67770Sewage plantGöttingenGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Biodegradation#Anaerobic digestor
#Engineered#Waste#Wastewater
#Condition#Anoxic (anaerobic)

Safety information

risk assessment

  • @ref: 1435
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Enterococcus faecalis clone OCM088 16S ribosomal RNA gene, partial sequenceGU417255462ena1351
20218Methanosarcina mazei Go1 16S ribosomal RNA gene, partial sequenceJQ3467571268ena192952

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
67770Methanosarcina mazei Go1GCA_000007065completencbi192952
66792Methanosarcina mazei Go1192952.21completepatric192952

GC content

  • @ref: 1435
  • GC-content: 41
  • method: sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno90.226no
gram-positiveno95.336no
anaerobicyes97.283yes
aerobicno93.994yes
halophileno88.126no
spore-formingno94.278no
glucose-utilyes66.774no
flagellatedno96.753no
thermophileno97.127no
glucose-fermentno71.692no

External links

@ref: 1435

culture collection no.: DSM 3647, ATCC BAA 159, JCM 11833, OCM 88

straininfo link

  • @ref: 76556
  • straininfo: 44307

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
1435Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3647)https://www.dsmz.de/collection/catalogue/details/culture/DSM-3647
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
76556Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID44307.1StrainInfo: A central database for resolving microbial strain identifiers