Strain identifier
BacDive ID: 7101
Type strain:
Species: Methanosarcina mazei
Strain Designation: Gö1
Strain history: DSM 3647 <-- F. Widdel Gö1.
NCBI tax ID(s): 2209 (species)
General
@ref: 1435
BacDive-ID: 7101
DSM-Number: 3647
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina mazei Gö1 is an anaerobe, mesophilic archaeon that was isolated from anaerobic sewage digester.
NCBI tax id
- NCBI tax id: 2209
- Matching level: species
strain history
@ref | history |
---|---|
1435 | <- F. Widdel, Gö1 (Methanosarcina sp.) |
67770 | DSM 3647 <-- F. Widdel Gö1. |
doi: 10.13145/bacdive7101.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina mazei
- full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
synonyms
@ref synonym 20215 Methanosarcina frisia 20215 Methanococcus frisius 20215 Methanosarcina mazeii 20215 Methanococcus mazei
@ref: 1435
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina mazei
full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984
strain designation: Gö1
type strain: no
Culture and growth conditions
culture medium
- @ref: 1435
- name: METHANOSARCINA MEDIUM (DSMZ Medium 120)
- growth: yes
- link: https://mediadive.dsmz.de/medium/120
- composition: Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
1435 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 1435
- oxygen tolerance: anaerobe
compound production
- @ref: 1435
- compound: ATPase
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
1435 | anaerobic sewage digester | Göttingen | Germany | DEU | Europe |
67770 | Sewage plant | Göttingen | Germany | DEU | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Biodegradation | #Anaerobic digestor |
#Engineered | #Waste | #Wastewater |
#Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 1435
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Enterococcus faecalis clone OCM088 16S ribosomal RNA gene, partial sequence | GU417255 | 462 | ena | 1351 |
20218 | Methanosarcina mazei Go1 16S ribosomal RNA gene, partial sequence | JQ346757 | 1268 | ena | 192952 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
67770 | Methanosarcina mazei Go1 | GCA_000007065 | complete | ncbi | 192952 |
66792 | Methanosarcina mazei Go1 | 192952.21 | complete | patric | 192952 |
GC content
- @ref: 1435
- GC-content: 41
- method: sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 90.226 | no |
gram-positive | no | 95.336 | no |
anaerobic | yes | 97.283 | yes |
aerobic | no | 93.994 | yes |
halophile | no | 88.126 | no |
spore-forming | no | 94.278 | no |
glucose-util | yes | 66.774 | no |
flagellated | no | 96.753 | no |
thermophile | no | 97.127 | no |
glucose-ferment | no | 71.692 | no |
External links
@ref: 1435
culture collection no.: DSM 3647, ATCC BAA 159, JCM 11833, OCM 88
straininfo link
- @ref: 76556
- straininfo: 44307
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
1435 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 3647) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-3647 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
76556 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID44307.1 | StrainInfo: A central database for resolving microbial strain identifiers |