Strain identifier
BacDive ID: 7096
Type strain: 
Species: Methanosarcina mazei
Strain Designation: S-6
Strain history: <- R. A. Mah, University of California, Los Angeles, USA; S-6
NCBI tax ID(s): 2209 (species)
version 8.1 (current version)
General
@ref: 690
BacDive-ID: 7096
DSM-Number: 2053
keywords: genome sequence, 16S sequence, Archaea, anaerobe, mesophilic
description: Methanosarcina mazei S-6 is an anaerobe, mesophilic archaeon that was isolated from sewage sludge plant.
NCBI tax id
- NCBI tax id: 2209
- Matching level: species
strain history
- @ref: 690
- history: <- R. A. Mah, University of California, Los Angeles, USA; S-6
doi: 10.13145/bacdive7096.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Methanomicrobia
- order: Methanosarcinales
- family: Methanosarcinaceae
- genus: Methanosarcina
- species: Methanosarcina mazei
- full scientific name: Methanosarcina mazei corrig. (Barker 1936) Mah and Kuhn 1984
synonyms
@ref synonym 20215 Methanosarcina frisia 20215 Methanococcus frisius 20215 Methanosarcina mazeii 20215 Methanococcus mazei
@ref: 690
domain: Archaea
phylum: Euryarchaeota
class: Methanomicrobia
order: Methanosarcinales
family: Methanosarcinaceae
genus: Methanosarcina
species: Methanosarcina mazei
full scientific name: Methanosarcina mazei (Barker 1936) Mah and Kuhn 1984
strain designation: S-6
type strain: yes
Morphology
multimedia
- @ref: 690
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_2053.jpg
- caption: Medium 120, 37°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
- @ref: 690
- name: METHANOSARCINA MEDIUM (DSMZ Medium 120)
- growth: yes
- link: https://mediadive.dsmz.de/medium/120
- composition: Name: METHANOSARCINA MEDIUM (DSMZ Medium 120) Composition: Methanol 15.4688 g/l Na-acetate 2.44141 g/l NaCl 2.19727 g/l NaHCO3 1.95312 g/l Casitone 1.95312 g/l Yeast extract 1.95312 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Thiamine HCl 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water
culture temp
- @ref: 690
- growth: positive
- type: growth
- temperature: 37
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 690
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 690
- sample type: sewage sludge plant
- geographic location: California, El Segundo
- country: USA
- origin.country: USA
- continent: North America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Industrial | #Plant (Factory) |
| #Engineered | #Waste | #Sewage sludge |
taxonmaps
- @ref: 69479
- File name: preview.99_187097.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17802;96_68776;97_89418;98_121696;99_187097&stattab=map
- Last taxonomy: Methanosarcina
- 16S sequence: AJ012095
- Sequence Identity:
- Total samples: 7202
- soil counts: 2215
- aquatic counts: 3372
- animal counts: 684
- plant counts: 931
Safety information
risk assessment
- @ref: 690
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Enterococcus faecalis clone OCM026 16S ribosomal RNA gene, partial sequence | GU417218 | 456 | ena | 1351 |
| 690 | Methanosarcina mazei 16S rRNA gene, strain DSM 2053 (T) | AJ012095 | 1333 | ena | 2209 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Methanosarcina mazei S-6 | GCA_000970205 | complete | ncbi | 213585 |
| 66792 | Methanosarcina mazei S-6 | 2627853861 | complete | img | 213585 |
| 66792 | Methanosarcina mazei S-6 | 213585.10 | complete | patric | 213585 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 90.768 | no |
| flagellated | no | 96.59 | no |
| gram-positive | no | 95.415 | no |
| anaerobic | yes | 97.716 | yes |
| aerobic | no | 94.922 | yes |
| halophile | no | 87.193 | no |
| spore-forming | no | 95.002 | no |
| thermophile | no | 96.032 | no |
| glucose-util | yes | 68.233 | no |
| glucose-ferment | no | 71.041 | no |
External links
@ref: 690
culture collection no.: DSM 2053, ATCC 43572, OCM 26, DSM 108209
straininfo link
- @ref: 76551
- straininfo: 41776
literature
- topic: Phylogeny
- Pubmed-ID: 23378113
- title: Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil.
- authors: Wagner D, Schirmack J, Ganzert L, Morozova D, Mangelsdorf K
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.046565-0
- year: 2013
- mesh: Base Composition, DNA, Archaeal/genetics, Methanosarcina/*classification/genetics/isolation & purification, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Siberia, *Soil Microbiology
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 690 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 2053) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-2053 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 76551 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41776.1 | StrainInfo: A central database for resolving microbial strain identifiers |