Pseudomonas fluorescens (DSM 50415, ATCC 12983, ICPB 2278)

Name: Pseudomonas fluorescens (DSM 50415, ATCC 12983, ICPB 2278)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 12858

Type strain

Strain Designation 1291, LA

Culture col. no. DSM 50415 ATCC 12983 ICPB 2278 NCIB 8917 1291 5 more

NCBI tax ID(s) 294

Special Collections DSMZ CIP

History DSMZ <- ICPB <- ATCC <- R. Hugh (Pseudomonas lemmonieri 1291) <- ATCC <- NCIB <- W.B. Hugo, LA CIP <- 1959, NCIB, Pseudomonas lemonnieri <- W. Hugo: strain LA

Links

Pseudomonas fluorescens 1291 is an obligate aerobe, Gram-negative, motile animal pathogen that was isolated from Environment, Soil, Keuper Marl.

Gram-negative motile rod-shaped obligate aerobe animal pathogen 16S sequence Bacteria

Name and taxonomic classification

SI-ID 4068

LMG 5939,ATCC 12983,ATCC 17458,CCUG 1255,DSM 50415,NCIB 8917,NCPPB 1802,NCTC 10690,NRRL B-1864,IFO 15837,NCIMB 8917,CIP 59.27,NBRC 15837,BCRC 13910,CCRC 13910,ICMP 13616,KACC 10323,NCIM 2060,CFBP 3150

@ref 20215

Last LPSN update 2026-05-29 08:27:54

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Pseudomonadales

Family Pseudomonadaceae

Genus Pseudomonas

Species Pseudomonas fluorescens

Full scientific name Pseudomonas fluorescens Migula 1895 (Approved Lists 1980)

BacDive ID	Other strains from **Pseudomonas fluorescens** (99)	Type strain
12851	P. fluorescens DSM 50090, ATCC 13525, ICPB 3200, NCIB 9046, ... (type strain)
12825	P. fluorescens 1175, DSM 1976
12827	P. fluorescens B599, DSM 2764
12828	P. fluorescens AR 11, DSM 4358, NCDO 2085
12829	P. fluorescens WS 1760, DSM 6147
12830	P. fluorescens ST, DSM 6290
12831	P. fluorescens EMK, DSM 6308
12833	P. fluorescens IRB110, DSM 6607
12834	P. fluorescens IRB120, DSM 6608
12835	P. fluorescens IRB130, DSM 6609
12836	P. fluorescens pUTK21, 5RL, DSM 6699
12837	P. fluorescens pUTK21, HK44, DSM 6700
12838	P. fluorescens AB VII, DSM 6757
12839	P. fluorescens PSC, DSM 7122
12840	P. fluorescens MBC 1, DSM 7153
12842	P. fluorescens PCR, DSM 7355
12843	P. fluorescens JD1, DSM 8542
12844	P. fluorescens JD2, DSM 8543
12845	P. fluorescens II, DSM 8369, NCIMB 8859
12846	P. fluorescens 4, DSM 8567
12848	P. fluorescens Tween B, DSM 9958
12850	P. fluorescens SK1, DSM 16274
12852	P. fluorescens 12, 216, DSM 50091, ATCC 17397, ICPB 2657-12, ...
12853	P. fluorescens 411, DSM 50106, ATCC 17826, ICPB 2711-411, ...
12854	P. fluorescens 108, 52-22, DSM 50108, ATCC 17482, CIP 104377, ...
12855	P. fluorescens DSM 50124, ATCC 17581, ICPB 2656-214
12856	P. fluorescens 15, DSM 50148, ATCC 17533, ICPB 2440-15
12859	P. fluorescens ES1-129-5, DSM 30511
12860	P. fluorescens 92, DSM 23471
128377	P. fluorescens SF002755,
24369	P. fluorescens PWD33, DSM 11531
24370	P. fluorescens PWD34, DSM 11532
24371	P. fluorescens DSM 50101, ATCC 17387, ICPB 2657-2, Stanier ...
24372	P. fluorescens PJ253, DSM 50120, ATCC 17561, ICPB 2656-194
134423	P. fluorescens BA3SM1, CIP 110551
134568	P. fluorescens B10, CIP 106483, ATCC 948, IFO 3903, CCEB ...
134777	P. fluorescens CIP 63.26, CCEB 488
135120	P. fluorescens PMW6, CIP 104683, ATCC 39005, NCIMB 11615
137905	P. fluorescens 3749, CIP 102156
137942	P. fluorescens 215-86, CIP 102245
138074	P. fluorescens 52-1C, CIP 104605, ATCC 15453
138894	P. fluorescens CIP 63.47, ATCC 6972, LMG 1799, NCIMB 8194, ...
139769	P. fluorescens B-1606, DSM 50161, ATCC 17636, ICPB 2805-269, ...
141244	P. fluorescens CCUG 369, ATCC 17567
141379	P. fluorescens CCUG 1320, ATCC 17562, LMG 5940
141398	P. fluorescens CCUG 1428, NCTC 10688
141468	P. fluorescens CCUG 2088, NRRL B-1123, ATCC 10757
141593	P. fluorescens CCUG 3367, ATCC 19373, ATCC 23323, NCTC 1381
143398	P. fluorescens CCUG 18892
145593	P. fluorescens CCUG 28921
145658	P. fluorescens CCUG 29164
145761	P. fluorescens CCUG 29521
145794	P. fluorescens CCUG 29606
145826	P. fluorescens CCUG 29727
146312	P. fluorescens CCUG 30871
146676	P. fluorescens CCUG 31774
146677	P. fluorescens CCUG 31775
146678	P. fluorescens CCUG 31776
146679	P. fluorescens CCUG 31777
146764	P. fluorescens CCUG 32081
146879	P. fluorescens CCUG 32348
146915	P. fluorescens CCUG 32444
146922	P. fluorescens CCUG 32456 A
147480	P. fluorescens CCUG 33665
147487	P. fluorescens CCUG 33687
147857	P. fluorescens CCUG 34393
147953	P. fluorescens CCUG 34574
147954	P. fluorescens CCUG 34575 A
148032	P. fluorescens CCUG 34712
148211	P. fluorescens CCUG 35042
148620	P. fluorescens CCUG 35733
148804	P. fluorescens CCUG 35958
151752	P. fluorescens CCUG 44791
151931	P. fluorescens CCUG 45242
153000	P. fluorescens CCUG 48229
153803	P. fluorescens CCUG 51106, ATCC 17386, LMG 5167, NCPPB 1804
154555	P. fluorescens CCUG 54396
157427	P. fluorescens IMI 346683
157428	P. fluorescens IMI 347502
157429	P. fluorescens IMI 347507
157430	P. fluorescens IMI 347511
157431	P. fluorescens IMI 347531
157432	P. fluorescens IMI 349019
157433	P. fluorescens IMI 347506
157801	P. fluorescens FW300-N2E2, DSM 106119
157802	P. fluorescens FW300-N1B4, DSM 106120
157803	P. fluorescens FW300-N2C3, DSM 106121
157804	P. fluorescens GW456-L13, DSM 106123
157805	P. fluorescens FW300-N2E3, DSM 106124
161307	P. fluorescens JCM 13057, ATCC 949, IFO 3507, NBRC 3507, ...
161308	P. fluorescens JCM 13059, ATCC 11253, NRRL B-1244
161474	P. fluorescens JCM 14347, ATCC 14150, IFO 15840, LMG 14675, ...
161958	P. fluorescens JCM 17186, FERM P-11027, NBRC 107686
162688	P. fluorescens JCM 20007, ATCC 15916, IAM 1008
162702	P. fluorescens JCM 20030, IAM 1051
162704	P. fluorescens JCM 20032, ATCC 15917, IAM 1055
162712	P. fluorescens JCM 20046, IAM 1092, IFO 12568, NBRC 12568
162722	P. fluorescens JCM 20064, ATCC 31419, IAM 1126
163414	P. fluorescens JCM 2779, AJ 2089, IAM 12405

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
119408	negative	rod-shaped

Pigmentation

@ref	Production	Name
119408		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
37344	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
12716	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
12716	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
12716	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
119408	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
12716	positive	growth	30
37344	positive	growth	30
119408	positive	growth	10-30
119408	negative	growth	5
119408	negative	growth	41

Physiology and metabolism

Oxygen tolerance

119408

Oxygen toleranceobligate aerobe

Compound production

@ref	Compound
12716	pyocyanin-like pigment
12716	poly(ß-hydroxyoctanoic acid)
12716	nitrite reductase
12716	nitrous oxide reductase

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	+	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
119408	17632 ChEBI	nitrate	-	reduction
119408	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
119408	16301 ChEBI	nitrite	-	reduction
119408	15882 ChEBI	phenol	+	degradation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
119408	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
119408	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
119408	alcohol dehydrogenase	+	1.1.1.1
119408	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
119408	beta-galactosidase	-	3.2.1.23
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
119408	caseinase	+	3.4.21.50
119408	catalase	+	1.11.1.6
119408	DNase	-
119408	gelatinase	+/-
68369	gelatinase	+		from API 20NE
119408	lecithinase	-
119408	lipase	-
119408	lysine decarboxylase	-	4.1.1.18
119408	ornithine decarboxylase	-	4.1.1.17
119408	oxidase	+
119408	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
12716	+	-	-	-	-	-	+	-	+	+	+	+	+	-	+	+	-	+	+	-	+
12716	+	-	-	-	-	-	+	-	+	+	+	+	+	-	+	+	-	+	+	-	+/-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
119408	not determinedn.d.	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	+	+	-	-	-	+	+	+	+	-	+	-	-	+	-	-	+	+	+	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	-	+	+	+	+	+	+	-	-	-	+	+	+	+	+	+	+	-	+	+	-

Isolation, sampling and environmental information

Isolation

119408

Sample typeEnvironment, Soil, Keuper Marl

Taxonmaps

69479

Global distribution of 16S sequence AJ308306 (>99% sequence identity) for Pseudomonas from Microbeatlas

Aquatic Samples	8731
Soil Samples	16904
Animal Samples	11521
Plant Samples	5158
Total Samples	42314

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
12716	yes, in single cases		1	Risk group (German classification) According to TRBA 466
119408			1	Risk group (French classification) According to TRBA 466

Phages

@ref	Name	Strain number
124042	Pseudomonas phage vB_PflM-PT-JD08	DSM 50415

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Pseudomonas fluorescens partial 16S rRNA gene, strain Biotype F	AJ308306	1371		294
124043	Pseudomonas fluorescens strain LMG5939 16S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, complete sequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequence.	AF127585	526		294

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Dual-Temperature Microbiological Control of Cellular Products: A Potential Impact for Bacterial Screening of Platelet Concentrates?	Vollmer T, Knabbe C, Dreier J.	Microorganisms	10.3390/microorganisms11092350	2023
Metabolism	Synthesis of poly-3-hydroxyalkanoates is a common feature of fluorescent pseudomonads.	Huisman GW, de Leeuw O, Eggink G, Witholt B.	Appl Environ Microbiol	10.1128/aem.55.8.1949-1954.1989	1989
	Pyrimidine Nucleotide Biosynthesis and Regulation in Pseudomonas lemonnieri.	Bodampati S, West TP.	Curr Microbiol	10.1007/s00284-024-03957-6	2024
	Genotypic characterization of Bradyrhizobium strains nodulating endemic woody legumes of the Canary Islands by PCR-restriction fragment length polymorphism analysis of genes encoding 16S rRNA (16S rDNA) and 16S-23S rDNA intergenic spacers, repetitive extragenic palindromic PCR genomic fingerprinting, and partial 16S rDNA sequencing.	Vinuesa P, Rademaker JL, de Bruijn FJ, Werner D.	Appl Environ Microbiol	10.1128/aem.64.6.2096-2104.1998	1998
	Effect of Two Plant Species, Flax (Linum usitatissinum L.) and Tomato (Lycopersicon esculentum Mill.), on the Diversity of Soilborne Populations of Fluorescent Pseudomonads.	Lemanceau P, Corberand T, Gardan L, Latour X, Laguerre G, Boeufgras J, Alabouvette C.	Appl Environ Microbiol	10.1128/aem.61.3.1004-1012.1995	1995
Metabolism	Role of the Tat ransport system in nitrous oxide reductase translocation and cytochrome cd1 biosynthesis in Pseudomonas stutzeri.	Heikkila MP, Honisch U, Wunsch P, Zumft WG.	J Bacteriol	10.1128/jb.183.5.1663-1671.2001	2001
	Sequence diversity of the oprI gene, coding for major outer membrane lipoprotein I, among rRNA group I pseudomonads.	De Vos D, Bouton C, Sarniguet A, De Vos P, Vauterin M, Cornelis P.	J Bacteriol	10.1128/jb.180.24.6551-6556.1998	1998
Enzymology	Purification and characterization of an exopolysaccharide of Burkholderia (Pseudomonas) pseudomallei.	Steinmetz I, Rohde M, Brenneke B.	Infect Immun	10.1128/iai.63.10.3959-3965.1995	1995

References

#12716	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 50415
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#37344	; Curators of the CIP;
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119408	Collection of Institut Pasteur ; Curators of the CIP; CIP 59.27
#124042	Johannes Wittmann, Clara Rolland, Lorenz Reimer, Joaquim Sardà: PhageDive .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Pseudomonas fluorescens (DSM 50415, ATCC 12983, ICPB 2278)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Phages

Sequence information

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

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