Pseudomonas fluorescens B10 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from Creamery waste.
Gram-negative motile rod-shaped aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
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|
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Pseudomonadales |
| Family Pseudomonadaceae |
| Genus Pseudomonas |
| Species Pseudomonas fluorescens |
| Full scientific name Pseudomonas fluorescens Migula 1895 (Approved Lists 1980) |
| @ref | Name | Growth | Composition | Medium link | |
|---|---|---|---|---|---|
| 32975 | MEDIUM 3 - Columbia agar | Columbia agar (39.000 g);distilled water (1000.000 ml) | |||
| 32975 | CIP Medium 72 | Medium recipe at CIP | |||
| 32975 | CIP Medium 3 | Medium recipe at CIP |
| @ref | Spore formation | Confidence | |
|---|---|---|---|
| 125439 | 99.6 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68369 | 17128 ChEBI | adipate | - | assimilation | from API 20NE |
| 68369 | 29016 ChEBI | arginine | + | hydrolysis | from API 20NE |
| 32975 | 16947 ChEBI | citrate | + | carbon source | |
| 68369 | 17634 ChEBI | D-glucose | + | assimilation | from API 20NE |
| 68369 | 17634 ChEBI | D-glucose | - | fermentation | from API 20NE |
| 68369 | 16899 ChEBI | D-mannitol | + | assimilation | from API 20NE |
| 68369 | 16024 ChEBI | D-mannose | + | assimilation | from API 20NE |
| 68369 | 27689 ChEBI | decanoate | + | assimilation | from API 20NE |
| 32975 | 4853 ChEBI | esculin | - | hydrolysis | |
| 68369 | 4853 ChEBI | esculin | - | hydrolysis | from API 20NE |
| 68369 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20NE |
| 68369 | 24265 ChEBI | gluconate | + | assimilation | from API 20NE |
| 68369 | 30849 ChEBI | L-arabinose | - | assimilation | from API 20NE |
| 68369 | 25115 ChEBI | malate | + | assimilation | from API 20NE |
| 68369 | 17306 ChEBI | maltose | + | assimilation | from API 20NE |
| 68369 | 59640 ChEBI | N-acetylglucosamine | + | assimilation | from API 20NE |
| 32975 | 17632 ChEBI | nitrate | + | reduction | |
| 32975 | 17632 ChEBI | nitrate | - | respiration | |
| 68369 | 17632 ChEBI | nitrate | + | reduction | from API 20NE |
| 32975 | 16301 ChEBI | nitrite | - | reduction | |
| 68369 | 27897 ChEBI | tryptophan | - | energy source | from API 20NE |
| 68369 | 16199 ChEBI | urea | - | hydrolysis | from API 20NE |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 32975 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 32975 | alcohol dehydrogenase | + | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 32975 | amylase | - | ||
| 68369 | arginine dihydrolase | + | 3.5.3.6 | from API 20NE |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 32975 | beta-galactosidase | - | 3.2.1.23 | |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68369 | beta-glucosidase | - | 3.2.1.21 | from API 20NE |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 32975 | caseinase | + | 3.4.21.50 | |
| 32975 | catalase | + | 1.11.1.6 | |
| 67770 | cholesterol esterase | 3.1.1.13 | ||
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 68369 | cytochrome oxidase | + | 1.9.3.1 | from API 20NE |
| 32975 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 32975 | gelatinase | +/- | ||
| 68369 | gelatinase | - | from API 20NE | |
| 32975 | lecithinase | + | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 32975 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 32975 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 32975 | ornithine decarboxylase | - | 4.1.1.17 | |
| 32975 | oxidase | + | ||
| 32975 | protease | - | ||
| 68382 | trypsin | + | 3.4.21.4 | from API zym |
| 32975 | tryptophan deaminase | - | ||
| 32975 | tween esterase | - | ||
| 32975 | urease | - | 3.5.1.5 | |
| 68369 | urease | - | 3.5.1.5 | from API 20NE |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Food production | #Dairy product | |
| #Engineered | #Industrial | #Industrial production | |
| #Engineered | #Waste | #Industrial waste |
Global distribution of 16S sequence LC507955 (>99% sequence identity) for Pseudomonas from Microbeatlas ![]()
| @ref | Biosafety level | Biosafety level comment | |
|---|---|---|---|
| 32975 | 1 | Risk group (French classification) |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 66792 | ATCC948-2 assembly for Pseudomonas fluorescens ATCC 948 substr. 2 | scaffold | 294 | |||
| 66792 | ATCC948-1 assembly for Pseudomonas fluorescens ATCC 948 substr. 1 | scaffold | 294 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 67770 | Pseudomonas fluorescens gene for 16S rRNA, partial sequence, strain: NBRC 3903 | AB680165 | 1462 | 294 | ||
| 67770 | Pseudomonas fluorescens strain LMG 5822 16S ribosomal RNA gene, partial sequence | GU198106 | 1500 | 294 | ||
| 67770 | Pseudomonas fluorescens JCM 13058 gene for 16S rRNA, partial sequence | LC507955 | 1459 | 294 | ||
| 124043 | Pseudomonas fluorescens strain BCRC 10304 16S-23S ribosomal RNA intergenic spacer, complete sequence. | EU014543 | 512 | 294 | ||
| 124043 | Pseudomonas fluorescens strain ATCC 948 16S ribosomal RNA gene, partial sequence. | KY435607 | 1370 | 294 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | no | 99.60 | no |
| 125439 | motility | BacteriaNetⓘ | yes | 89.90 | no |
| 125439 | gram_stain | BacteriaNetⓘ | negative | 99.80 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 91.00 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | no | 98.50 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 98.28 | no |
| 125438 | aerobic | aerobicⓘ | yes | 91.56 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 87.80 | no |
| 125438 | thermophilic | thermophileⓘ | no | 98.50 | yes |
| 125438 | flagellated | motile2+ⓘ | yes | 87.41 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Genetics | Mining Marine Metagenomes Revealed a Quorum-Quenching Lactonase with Improved Biochemical Properties That Inhibits the Food Spoilage Bacterium Pseudomonas fluorescens. | Haramati R, Dor S, Gurevich D, Levy D, Freund D, Rytwo G, Sharon I, Afriat-Jurnou L. | Appl Environ Microbiol | 10.1128/aem.01680-21 | 2022 | |
| Kinetic models for pulsed electric field and thermal inactivation of Escherichia coli and Pseudomonas fluorescens in whole milk | Walter L, Knight G, Ng SY, Buckow R. | Int Dairy J | 10.1016/j.idairyj.2016.01.027 | 2016 | ||
| Architectural Features and Resistance to Food-Grade Disinfectants in Listeria monocytogenes-Pseudomonas spp. Dual-Species Biofilms. | Rodriguez-Lopez P, Rodriguez-Herrera JJ, Lopez Cabo M. | Front Microbiol | 10.3389/fmicb.2022.917964 | 2022 | ||
| The Use of Ozone as an Eco-Friendly Strategy against Microbial Biofilm in Dairy Manufacturing Plants: A Review. | Panebianco F, Rubiola S, Di Ciccio PA. | Microorganisms | 10.3390/microorganisms10010162 | 2022 | ||
| Strategies for oral delivery of bioactive peptides with focus on debittering and masking. | Mirzapour-Kouhdasht A, McClements DJ, Taghizadeh MS, Niazi A, Garcia-Vaquero M. | NPJ Sci Food | 10.1038/s41538-023-00198-y | 2023 | ||
| Purification and characterization of an endopeptidase from Pseudomonas fluorescens ATCC 948 | Gobbetti M, Smacchi E, Stepaniak L, Crea F, Fox PF. | Journal of Food Biochemistry. | 10.1111/j.1745-4514.1998.tb00228.x | 1998 | ||
| Enzymology | Cloning, sequencing, and expression in Escherichia coli of the D-hydantoinase gene from Pseudomonas putida and distribution of homologous genes in other microorganisms. | LaPointe G, Viau S, LeBlanc D, Robert N, Morin A. | Appl Environ Microbiol | 10.1128/aem.60.3.888-895.1994 | 1994 | |
| Lipid patterns of selected microorganisms as determined by gas-liquid chromatography. | Steinhauer JE, Flentge RL, Lechowich RV. | Appl Microbiol | 10.1128/am.15.4.826-829.1967 | 1967 | ||
| Metabolism | Bioactive Compounds of Pseudoalteromonas sp. IBRL PD4.8 Inhibit Growth of Fouling Bacteria and Attenuate Biofilms of Vibrio alginolyticus FB3. | Supardy NA, Ibrahim D, Mat Nor SR, Noordin WNM. | Pol J Microbiol | 10.21307/pjm-2019-003 | 2019 | |
| Catalase activity of psychrophilic bacteria grown at 2 and at 30 C. | FRANK HA, ISHIBASHI ST, REID A, ITO JS. | Appl Microbiol | 10.1128/am.11.2.151-153.1963 | 1963 | ||
| Steroids. 159. Antimicrobial properties of 21,21-dimethoxy progesterone and other progesterone analogues. | CASAS-CAMPILLO C, BALANDRANO D, GALARZA A. | J Bacteriol | 10.1128/jb.81.3.366-375.1961 | 1961 | ||
| Reduction in Spoilage Microbiota and Cyclopiazonic Acid Mycotoxin with Chestnut Extract Enriched Chitosan Packaging: Stability of Inoculated Gouda Cheese. | Korge K, Seme H, Bajic M, Likozar B, Novak U. | Foods | 10.3390/foods9111645 | 2020 | ||
| Cultivation | Media for the enhancement of fluorescent pigment production by Pseudomonas species. | Garibaldi JA. | J Bacteriol | 10.1128/jb.94.5.1296-1299.1967 | 1967 | |
| Metabolism | Lipolytic activity of various microorganisms isolated from cottonseed. | MAYNE RY. | Appl Microbiol | 10.1128/am.4.5.270-273.1956 | 1956 | |
| Metabolism | Production and comparison of peptide siderophores from strains of distantly related pathovars of Pseudomonas syringae and Pseudomonas viridiflava LMG 2352. | Bultreys A, Gheysen I. | Appl Environ Microbiol | 10.1128/aem.66.1.325-331.2000 | 2000 | |
| Pathogenicity | Mutation rate, spectrum, topology, and context-dependency in the DNA mismatch repair-deficient Pseudomonas fluorescens ATCC948. | Long H, Sung W, Miller SF, Ackerman MS, Doak TG, Lynch M | Genome Biol Evol | 10.1093/gbe/evu284 | 2014 | |
| Enzymology | Characterization and partial purification of a bacteriocin-like substance produced by thermophilic Bacillus licheniformis H1 isolated from cow manure compost. | Abdel-Mohsein HS, Sasaki T, Tada C, Nakai Y | Anim Sci J | 10.1111/j.1740-0929.2010.00835.x | 2011 | |
| Biotechnology | Bactericidal activity of lactoferrin and its amidated and pepsin-digested derivatives against Pseudomonas fluorescens in ground beef and meat fractions. | del Olmo A, Morales P, Nunez M | J Food Prot | 10.4315/0362-028x-72.4.760 | 2009 | |
| Pathogenicity | Antimicrobial activity of lactoperoxidase system incorporated into cross-linked alginate films. | Yener FY, Korel F, Yemenicioglu A | J Food Sci | 10.1111/j.1750-3841.2009.01057.x | 2009 | |
| Enzymology | Inhibition of Proteolytic Enzymes from Pseudomonas fluorescens ATCC 948 and Angiotensin I-Converting Enzyme by Peptides from Zein, Hordein, and Gluten Hydrolysates. | Gobbetti M, Smacchi E, Corsetti A, Bellucci M | J Food Prot | 10.4315/0362-028X-60.5.499 | 1997 | |
| Enzymology | Purification and characterization of a dipeptidase from Pseudomonas fluorescens ATCC 948. | Gobbetti M, Fox PF | J Dairy Sci | 10.3168/jds.S0022-0302(96)76372-5 | 1996 | |
| Cultivation | Peptidase profiles of Pseudomonas fluorescens: identification and properties. | Gobbetti M, Rossi J | J Dairy Sci | 10.3168/jds.S0022-0302(92)77832-1 | 1992 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #32975 | Collection of Institut Pasteur ; Curators of the CIP; CIP 106483 |
| #44346 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 1254 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68369 | Automatically annotated from API 20NE . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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