Name: Pseudomonas fluorescens pUTK21
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 12837

Type strain:

Species: Pseudomonas fluorescens

Strain Designation: pUTK21, HK44

Culture col. no.: DSM 6700

NCBI tax ID(s): 294 (species)

SI-ID 113081

DSM 6700

Special Collections

DSMZ

Other strains from Pseudomonas fluorescens

Section

Pseudomonas fluorescens pUTK21 is a Gram-negative human pathogen of the family Pseudomonadaceae.

Gram-negative
human pathogen
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Pseudomonadaceae*
	Genus	*Pseudomonas*
	Species	**Pseudomonas fluorescens**
	Full Scientific Name (LPSN)	Pseudomonas fluorescens Migula 1895 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Oxygen tolerance

aerobe Confidence in %92.478

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2777]	24376	+	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	+	+	+	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2777]	Pathogenicity (human)	yes
[Ref.: #2777]	Pathogenicity (animal)	yes
[Ref.: #2777]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudomonas fluorescens HK44	GCA_000217955	scaffold	GenBank ENA	1042209 tax ID	*
[Ref.: #66792]	Pseudomonas fluorescens HK44	1042209.11	wgs	PATRIC	1042209 tax ID	*
[Ref.: #66792]	Pseudomonas fluorescens HK44	2504643024	draft	JGI IMG/M	1042209 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Pseudomonas fluorescens HK44 PATRIC: 1042209.11
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudomonas fluorescens HK44 HK44 NCBI: GCA_000217955.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	99.5	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	98.544	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	92.478	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.439	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98.486	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	84.903	no

Availability in culture collections External links

[Ref.: #2777]

Culture collection no.

DSM 6700

[Ref.: #82049]

SI-ID 113081

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft genome sequence of the polycyclic aromatic hydrocarbon-degrading, genetically engineered bioluminescent bioreporter Pseudomonas fluorescens HK44.	Chauhan A, Layton AC, Williams DE, Smartt AE, Ripp S, Karpinets TV, Brown SD, Sayler GS	J Bacteriol	10.1128/JB.05530-11	2011	*
Metabolism	Comparison of response of six different luminescent bacterial bioassays to bioremediation of five contrasting oils.	Bundy JG, Campbell CD, Paton GI	J Environ Monit	10.1039/b103104j	2001	*
Metabolism	Development of QSARs to investigate the bacterial toxicity and biotransformation potential of aromatic heterocylic compounds.	Bundy JG, Morriss AW, Durham DG, Campbell CD, Paton GI	Chemosphere	10.1016/s0045-6535(00)00178-8	2001	*
Enzymology	Kinetics and response of a Pseudomonas fuorescens HK44 biosensor.	Webb OF, Bienkowski PR, Matrubutham U, Evans FA, Heitzer A, Sayler GS	Biotechnol Bioeng	10.1002/(SICI)1097-0290(19970605)54:5<491::AID-BIT8>3.0.CO;2-9	1997	*
Metabolism	NAH plasmid-mediated catabolism of anthracene and phenanthrene to naphthoic acids.	Menn FM, Applegate BM, Sayler GS	Appl Environ Microbiol	10.1128/aem.59.6.1938-1942.1993	1993	*
Metabolism	Rapid, sensitive bioluminescent reporter technology for naphthalene exposure and biodegradation.	King JM, Digrazia PM, Applegate B, Burlage R, Sanseverino J, Dunbar P, Larimer F, Sayler GS	Science	10.1126/science.249.4970.778	1990	*

References

#2777

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6700

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#82049

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID113081.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Pseudomonas fluorescens in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Pseudomonas fluorescens HK44

Pseudomonas fluorescens HK44 HK44

Change proposal

Change proposal