Haemophilus influenzae Kilian HK 394 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from human cerebrospinal fluid.
Gram-negative rod-shaped microaerophile genome sequence Bacteria| @ref 20215 |
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Last LPSN update
2026-02-09 11:20:38 |
| Domain Bacteria |
| Phylum Pseudomonadota |
| Class Gammaproteobacteria |
| Order Pasteurellales |
| Family Pasteurellaceae |
| Genus Haemophilus |
| Species Haemophilus influenzae |
| Full scientific name Haemophilus influenzae corrig. (Lehmann and Neumann 1896) Winslow et al. 1917 (Approved Lists 1980) |
| Synonyms (2) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 16829 | HAEMOPHILUS MEDIUM (DSMZ Medium 804) | Medium recipe at MediaDive  | Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water | ||
| 36559 | MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria | Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml) | |||
| 120108 | CIP Medium 10 | Medium recipe at CIP |
| Test 1 | Test 2 | Test 3 | Test 4 | Test 5 | |
|---|---|---|---|---|---|
| @ref | 16829 | 16829 | 16829 | 16829 | 16829 |
| Medium | Mueller-Hinton Agar | M 804 | M 804 | M 804 | M 804 |
| Manual annotation | 1 | 1 | 1 | 1 | 1 |
| Inhibition zone diameter in mm | Inhibition zone diameter in mm | Inhibition zone diameter in mm | Inhibition zone diameter in mm | Inhibition zone diameter in mm | |
| Amikacin 30µg (disc) | 24 | 24-26 | 24 | 18 | 18 |
| Ampicillin 10µg (disc) | 34 | 34 | 36-38 | 20-22 | 20-22 |
| Aztreonam 30µg (disc) | 42 | 42 | 44-46 | 32 | 32 |
| Bacitracin 10Unit | 0 | 0 | 10 | 0 | 0 |
| Cefalotin 30µg (disc) | 30 | 30 | 30 | 20 | 20 |
| Cefazolin 30µg (disc) | 28-30 | 28-30 | 30 | 12 | 12 |
| Cefotaxime 30µg (disc) | 46 | 46-48 | 48 | 32 | 32 |
| Ceftriaxone 30µg (disc) | 44 | 46-48 | 50 | 32 | 32 |
| Chloramphenicol 30µg (disc) | 42 | 50 | 48-50 | 32-34 | 32-34 |
| Clindamycin 10µg (disc) | 20 | 26 | 30 | 10-12 | 10-12 |
| Colistin 10µg (disc) | 20 | 20 | 20-22 | 14 | 14 |
| Doxycycline 30µg (disc) | 32 | 36-38 | 40 | 24-16 | 24-16 |
| Erythromycin 15µg (disc) | 22-24 | 20 | 20-22 | 12 | 12 |
| Fosfomycin 50µg (disc) | 48 | >50 | 46-48 | 28 | 28 |
| Gentamycin 10µg (disc) | |||||
| Imipenem 10µg (disc) | 36 | 38 | 36-38 | 22-24 | 22-24 |
| Kanamycin 30µg (disc) | 26 | 30 | 26 | 20 | 20 |
| Lincomycin 15µg (disc) | 12 | 14-16 | 14-16 | 8 | 8 |
| Linezolid 10µg (disc) | 22 | 26 | 18-20 | 12 | 12 |
| Mezlocillin 30µg (disc) | 38 | 38 | 36-38 | 24 | 24 |
| Moxifloxacin 5µg (disc) | 36-38 | 40 | 40 | 28 | 28 |
| Neomycin 30µg (disc) | 20 | 22 | 18 | 16 | 16 |
| Nitrofurantoin 100µg (disc) | 32 | 40-42 | 36 | 20-22 | 20-22 |
| Norfloxacin 10µg (disc) | 40 | 40 | 40-42 | 30 | 30 |
| Nystatin 100Unit | 8 | 0 | 8 | 0 | 0 |
| Ofloxacin 5µg (disc) | 40 | 40 | 42 | 30 | 30 |
| Oxacillin 5µg (disc) | 0 | 0 | 0 | 0 | 0 |
| Penicillin G 6µg (disc) | 30 | 30 | 30-32 | 20 | 20 |
| Pipemidic acid 20µg (disc) | 26 | 28 | 28 | 20 | 20 |
| Piperacillin/Tazobactam 40µg (disc) | 38 | 40 | 40 | 24 | 24 |
| Polymyxin B 300Unit | 20 | 20 | 22 | 16 | 16 |
| Quinupristin/Dalfopristin 15µg (disc) | 20 | 22 | 20 | 16 | 16 |
| Teicoplanin 30µg (disc) | 8 | 8 | 10 | 6 | 6 |
| Tetracycline 30µg (disc) | 40 | 40-42 | 40 | 28 | 28 |
| Ticarcillin 75µg (disc) | 42 | 40 | 44-46 | 30 | 30 |
| Vancomycin 30µg (disc) | 6 | 8 | 10-12 | 6 | 6 |
| @ref | ChEBI | Metabolite | Is resistant | Resistance conc. | |
|---|---|---|---|---|---|
| 16829 | 7809 | Oxacillin | 5 µg (disc) | from Antibiotic test |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | - | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 68377 | beta-galactosidase | - | 3.2.1.23 | from API NH |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 16829 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 16829 | cytochrome-c oxidase | + | 1.9.3.1 | |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | - | from API zym | |
| 68377 | gamma-glutamyltransferase | - | 2.3.2.2 | from API NH |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 68377 | proline-arylamidase | - | 3.4.11.5 | from API NH |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 68377 | tryptophan deaminase | - | 4.1.99.1 | from API NH |
| 68382 | valine arylamidase | - | from API zym |
Metadata FA analysis  | |||||||||||||||||||||||||||||||||||||||||||
| @ref | 65609 | ||||||||||||||||||||||||||||||||||||||||||
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| @ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 65609 | - | + | + | - | - | + | + | - | + | - | - | - | - | |
| 16829 | + | + | +/- | +/- | + | + | - | + | - | - | - | - | - | |
| 16829 | + | + | + | + | + | + | - | + | - | - | - | - | - | |
| 16829 | + | + | - | - | + | + | - | + | - | - | - | - | - | |
| 16829 | + | + | +/- | +/- | - | + | - | + | - | - | - | - | - | |
| 16829 | - | + | + | - | +/- | + | + | - | + | - | - | - | - | |
| 16829 | - | + | + | + | + | + | + | - | + | - | - | - | - | |
| 16829 | - | + | + | +/- | +/- | + | + | - | + | - | - | - | - | |
| 16829 | - | + | + | - | - | + | + | - | + | - | - | - | - | |
| 16829 | - | + | + | +/- | +/- | - | + | - | + | - | - | - | - |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|
| 66792 | 35860_G01 assembly for Haemophilus influenzae NCTC8468 | complete | GCA_901472485   | 727.2184  | 727 | 98.56 | |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Establishment and Clinical Application of a RPA-LFS Assay for Detection of Capsulated and Non-Capsulated Haemophilus influenzae. | Wang Y, Liu A, Fu M, Guo J, Wang L, Zuo X, Ma F. | Front Cell Infect Microbiol | 10.3389/fcimb.2022.878813 | 2022 | | |
| Epidemiological analysis and rapid detection by one-step multiplex PCR assay of Haemophilus influenzae in children with respiratory tract infections in Zhejiang Province, China. | Fan X, Liu X, Ji L, Cai D, Jiang J, Zhu J, Sun A, Yan J. | BMC Infect Dis | 10.1186/s12879-018-3295-2 | 2018 | | |
| Phylogeny | Clinical and Bacteriologic Analysis of Nontypeable Haemophilus influenzae Strains Isolated from Children with Invasive Diseases in Japan from 2008 to 2015. | Naito S, Takeuchi N, Ohkusu M, Takahashi-Nakaguchi A, Takahashi H, Imuta N, Nishi J, Shibayama K, Matsuoka M, Sasaki Y, Ishiwada N. | J Clin Microbiol | 10.1128/jcm.00141-18 | 2018 | |
| Pathogenicity | Mycoplasma pneumoniae Compared to Streptococcus pneumoniae Avoids Induction of Proinflammatory Epithelial Cell Responses despite Robustly Inducing TLR2 Signaling. | de Groot RCA, Zhu H, Hoogenboezem T, de Bruijn ACJM, Eenjes E, 't Jong AEJ, Belo AI, Estevao SC, Bajramovic JJ, Rottier RJ, Kool M, van Rossum AMC, Unger WWJ. | Infect Immun | 10.1128/iai.00129-22 | 2022 | |
| Pathogenicity | In vitro and in vivo activities of a new cephalosporin, FR264205, against Pseudomonas aeruginosa. | Takeda S, Nakai T, Wakai Y, Ikeda F, Hatano K. | Antimicrob Agents Chemother | 10.1128/aac.00860-06 | 2007 | |
| Pathogenicity | Novel carbapenem antibiotics for parenteral and oral applications: in vitro and in vivo activities of 2-aryl carbapenems and their pharmacokinetics in laboratory animals. | Fujimoto K, Takemoto K, Hatano K, Nakai T, Terashita S, Matsumoto M, Eriguchi Y, Eguchi K, Shimizudani T, Sato K, Kanazawa K, Sunagawa M, Ueda Y. | Antimicrob Agents Chemother | 10.1128/aac.01051-12 | 2013 | |
| Localization and density of Porphyromonas gingivalis and Tannerella forsythia in gingival and subgingival granulation tissues affected by chronic or aggressive periodontitis. | Rajakaruna GA, Negi M, Uchida K, Sekine M, Furukawa A, Ito T, Kobayashi D, Suzuki Y, Akashi T, Umeda M, Meinzer W, Izumi Y, Eishi Y. | Sci Rep | 10.1038/s41598-018-27766-7 | 2018 | | |
| Pathogenicity | Detection of multidrug resistance in Mycobacterium tuberculosis. | Sekiguchi J, Miyoshi-Akiyama T, Augustynowicz-Kopec E, Zwolska Z, Kirikae F, Toyota E, Kobayashi I, Morita K, Kudo K, Kato S, Kuratsuji T, Mori T, Kirikae T. | J Clin Microbiol | 10.1128/jcm.00750-06 | 2007 | |
| Development and evaluation of a line probe assay for rapid identification of pncA mutations in pyrazinamide-resistant mycobacterium tuberculosis strains. | Sekiguchi J, Nakamura T, Miyoshi-Akiyama T, Kirikae F, Kobayashi I, Augustynowicz-Kopec E, Zwolska Z, Morita K, Suetake T, Yoshida H, Kato S, Mori T, Kirikae T. | J Clin Microbiol | 10.1128/jcm.00352-07 | 2007 | | |
| Cultivation | Selective culture medium to survey the incidence of Haemophilus species. | Crawford JJ, Barden L, Kirkman JB. | Appl Microbiol | 10.1128/am.18.4.646-649.1969 | 1969 | |
| Enzymology | Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae and Neisseria meningitidis. | Abdeldaim GM, Stralin K, Korsgaard J, Blomberg J, Welinder-Olsson C, Herrmann B. | BMC Microbiol | 10.1186/1471-2180-10-310 | 2010 | |
| In vitro effect of ultrasound on bacteria and suggested protocol for sonication and diagnosis of prosthetic infections. | Monsen T, Lovgren E, Widerstrom M, Wallinder L. | J Clin Microbiol | 10.1128/jcm.02316-08 | 2009 | | |
| Enzymology | Simultaneous detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae by use of molecular beacons in a duplex real-time PCR. | Gullsby K, Storm M, Bondeson K. | J Clin Microbiol | 10.1128/jcm.01540-07 | 2008 | |
| Phylogeny | Direct and rapid identification and genogrouping of meningococci and porA amplification by LightCycler PCR. | Molling P, Jacobsson S, Backman A, Olcen P. | J Clin Microbiol | 10.1128/jcm.40.12.4531-4535.2002 | 2002 | |
| Genetics | Haemophilus influenzae Type b Meningitis in Infants, New York, New York, USA, 2022-2023. | Ewing A, Haldeman S, Job MJ, Otto C, Ratner AJ. | Emerg Infect Dis | 10.3201/eid3103.240946 | 2025 | |
| The lack of specificity of sodC-based PCR for the detection of Neisseria meningitidis carriage in pharyngeal swabs from adolescents. | Pham-Tran DD, Leong LEX, McMillan M, Lawrence A, Mohammed H, Finn A, Marshall HS. | Microbiol Spectr | 10.1128/spectrum.02779-24 | 2025 | | |
| Short incubation of disc diffusion for Streptococcus pneumoniae and Haemophilus influenzae to reduce time to susceptibility report. | Akerlund A, Serrander L, Sundqvist M. | J Antimicrob Chemother | 10.1093/jac/dkad272 | 2023 | | |
| Genetics | hicap: In Silico Serotyping of the Haemophilus influenzae Capsule Locus. | Watts SC, Holt KE. | J Clin Microbiol | 10.1128/jcm.00190-19 | 2019 | |
| Pathogenicity | Evaluation of an Automated System for Reading and Interpreting Disk Diffusion Antimicrobial Susceptibility Testing of Fastidious Bacteria. | Idelevich EA, Becker K, Schmitz J, Knaack D, Peters G, Kock R. | PLoS One | 10.1371/journal.pone.0159183 | 2016 | |
| Pathogenicity | Haemophilus influenzae with Non-Beta-Lactamase-Mediated Beta-Lactam Resistance: Easy To Find but Hard To Categorize. | Skaare D, Lia A, Hannisdal A, Tveten Y, Matuschek E, Kahlmeter G, Kristiansen BE. | J Clin Microbiol | 10.1128/jcm.01630-15 | 2015 | |
| Osteoarticular Infection in Three Young Thoroughbred Horses Caused by a Novel Gram Negative Cocco-Bacillus. | Hudson BJ, Chicken C, Blishen A, Todhunter KH, Begg AP, Chan L, Karagiannis T, Raymond B, Bogema D, Adkins AR, O'Sullivan CB, O'Rourke BA, Roy Chowdhury P, Djordjevic SP, Charles IG, Edgar A, Mitsakos K. | Case Rep Vet Med | 10.1155/2020/9785861 | 2020 | | |
| Enzymology | Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections. | Gadsby NJ, McHugh MP, Russell CD, Mark H, Conway Morris A, Laurenson IF, Hill AT, Templeton KE. | Clin Microbiol Infect | 10.1016/j.cmi.2015.05.004 | 2015 | |
| Direct blood culturing on solid medium outperforms an automated continuously monitored broth-based blood culture system in terms of time to identification and susceptibility testing. | Idelevich EA, Grunastel B, Peters G, Becker K. | New Microbes New Infect | 10.1016/j.nmni.2015.12.004 | 2016 | | |
| The gene encoding protein D (hpd) is highly conserved among Haemophilus influenzae type b and nontypeable strains. | Song XM, Forsgren A, Janson H. | Infect Immun | 10.1128/iai.63.2.696-699.1995 | 1995 | | |
| Pathogenicity | A wide spectrum of fastidious and ampicillin-susceptible bacteria dominate in animal-caused wounds. | Gustavsson O, Johansson AV, Monstein HJ, Nilsson LE, Bredberg A. | Eur J Clin Microbiol Infect Dis | 10.1007/s10096-016-2667-z | 2016 | |
| Pathogenicity | Laboratory- and species-specific interpretive breakpoints for disk diffusion tests of chloramphenicol susceptibility of Haemophilus influenzae. | Kronvall G, Ringertz S, Karlsson I, Goransson E, Dornbusch K. | Antimicrob Agents Chemother | 10.1128/aac.32.10.1484 | 1988 | |
| Pathogenicity | Amoxicillin treatment of experimental acute otitis media caused by Haemophilus influenzae with non-beta-lactamase-mediated resistance to beta-lactams: aspects of virulence and treatment. | Melhus A, Janson H, Westman E, Hermansson A, Forsgren A, Prellner K. | Antimicrob Agents Chemother | 10.1128/aac.41.9.1979 | 1997 | |
| Haemophilus influenzae resides and multiplies intracellularly in human adenoid tissue as demonstrated by in situ hybridization and bacterial viability assay. | Forsgren J, Samuelson A, Ahlin A, Jonasson J, Rynnel-Dagoo B, Lindberg A. | Infect Immun | 10.1128/iai.62.2.673-679.1994 | 1994 | | |
| Genetic characterization of a cryptic genospecies of Haemophilus causing urogenital and neonatal infections. | Quentin R, Martin C, Musser JM, Pasquier-Picard N, Goudeau A. | J Clin Microbiol | 10.1128/jcm.31.5.1111-1116.1993 | 1993 | | |
| Phylogeny | Genetic identification of cryptic genospecies of Haemophilus causing urogenital and neonatal infections by PCR using specific primers targeting genes coding for 16S rRNA. | Quentin R, Ruimy R, Rosenau A, Musser JM, Christen R. | J Clin Microbiol | 10.1128/jcm.34.6.1380-1385.1996 | 1996 | |
| Phylogeny | Capsulation in distantly related strains of Haemophilus influenzae type b: genetic drift and gene transfer at the capsulation locus. | Kroll JS, Moxon ER. | J Bacteriol | 10.1128/jb.172.3.1374-1379.1990 | 1990 | |
| Common antigenic domains in transferrin-binding protein 2 of Neisseria meningitidis, Neisseria gonorrhoeae, and Haemophilus influenzae type b. | Stevenson P, Williams P, Griffiths E. | Infect Immun | 10.1128/iai.60.6.2391-2396.1992 | 1992 | | |
| Pathogenicity | Pharmacodynamics of telithromycin In vitro against respiratory tract pathogens. | Odenholt I, Lowdin E, Cars O. | Antimicrob Agents Chemother | 10.1128/aac.45.1.23-29.2001 | 2001 | |
| Production of polyclonal and monoclonal antibodies against group A, B, and C capsular polysaccharides of Neisseria meningitidis and preparation of latex reagents. | Nato F, Mazie JC, Fournier JM, Slizewicz B, Sagot N, Guibourdenche M, Postic D, Riou JY. | J Clin Microbiol | 10.1128/jcm.29.7.1447-1452.1991 | 1991 | | |
| Pathogenicity | An invasive Haemophilus influenzae serotype b infection in an Anglo-Saxon plague victim. | Guellil M, Keller M, Dittmar JM, Inskip SA, Cessford C, Solnik A, Kivisild T, Metspalu M, Robb JE, Scheib CL | Genome Biol | 10.1186/s13059-021-02580-z | 2022 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive11704.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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