Name: Haemophilus influenzae L-378
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 11703

Type strain:

Species: Haemophilus influenzae

Strain Designation: L-378

Culture col. no.: CCUG 29539, DSM 11970, ATCC 49766, CIP 103570, CCM 4457, NCTC 12975

Strain history: CIP <- 1992, J.H. Jorgensen, Univ. Texas Hlth. Sci. Center, San-Antonio, Texas, USA <- 1986, S. Farmer, Med. College Wisconsin, USA

NCBI tax ID(s): 727 (species)

SI-ID 7367

LMG 13189, ATCC 49766, CCUG 29539, CIP 103570, CCM 4457, NCTC 12975, DSM 11970, CNCTC 5105, BCRC 17029, CCRC 17029, ACM 5055

Special Collections

Other strains from Haemophilus influenzae

Section

Haemophilus influenzae L-378 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from lung abcess.

Gram-negative
rod-shaped
microaerophile
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pasteurellales*
	Family	*Pasteurellaceae*
	Genus	*Haemophilus*
	Species	**Haemophilus influenzae**
	Full Scientific Name (LPSN)	Haemophilus influenzae corrig. (Lehmann and Neumann 1896) Winslow et al. 1917 (Approved Lists 1980)

	Synonym	Hemophilus influenzae
	Synonym	"Bacterium* influenzae"*

Information on morphological and physiological properties Morphology

[Ref.: #120111]	Gram stain	negative

[Ref.: #120111]	Cell shape	rod-shaped

[Ref.: #120111]	Motility	no

[Ref.: #4562]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4562]

Culture medium

HAEMOPHILUS MEDIUM (DSMZ Medium 804)

[Ref.: #4562]

Culture medium growth

[Ref.: #4562]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4562]

Culture medium composition

expand / minimize

[Ref.: #41776]

Culture medium

MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and Neisseria

[Ref.: #41776]

Culture medium growth

[Ref.: #41776]

Culture medium composition

expand / minimize

[Ref.: #120111]

Culture medium

CIP Medium 10

[Ref.: #120111]

Culture medium growth

[Ref.: #120111]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120111]

Culture medium

CIP Medium 10_BIORAD

[Ref.: #120111]

Culture medium growth

[Ref.: #120111]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4562]		growth	37 °C
[Ref.: #41776]		growth	37 °C
[Ref.: #120111]		growth	37 °C
[Ref.: #120111]	no	growth	30 °C
[Ref.: #120111]	no	growth	41 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4562]	Oxygen tolerance	microaerophile
[Ref.: #120111]	Oxygen tolerance	facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Reference

[Ref.: #4562]
M 804
37
1
microaerophil
yes
Inhibition zone diameter in mm
30-32
8
32-34
40
40-42
32
30
44
40-42
32
34-36
40
22
24
8
14
14-16
34-36
32-34
20
24
38
6
20
26-28
18
30
44
20
>50
34-36
24
resistant0
18
6
42

[Ref.: #4562]
M 804
37

microaerophil
yes
Inhibition zone diameter in mm
30
6
32
42-44
40
32
28
44-46
42
30
38
40-42
24-26
26
10
18-20
18
40
40
20
24
34-36
resistant0
20
30
22
32
44
18-20
>50
38
24
resistant0
20-22
6
44

[Ref.: #4562]
M 804



yes
Inhibition zone diameter in mm
30
10
30-32
40
40
32
30
42-44
40
32
36-38
40
24
24-26
resistant0
14-16
14-16
34
34-36
20-22
26
40-42
resistant0
20
24-26
20
32
46-48
16
>50
34-36
18
12
18-20
resistant0
40-42

[Ref.: #4562]
M 804
37

microaerophil
yes
Inhibition zone diameter in mm
28
8
26-28
38
34
30
22-24
40
38-40
28
28-30
34-36
20
20
resistant0
12
10
32-34
32
18
20-22
26
resistant0
16
22
16-18
24
40-42
16
46-48
30-32
18
resistant0
14-16
6-8
36

[Ref.: #4562]
M 804
37

microaerophil
yes
Inhibition zone diameter in mm
28
8
26-28
38
34
30
22-24
40
38-40
28
28-30
34-36
20
20
resistant0
12
10
32-34
32
18
20-22
26
resistant0
16
22
16-18
24
40-42
16
46-48
30-32
18
resistant0
14-16
6-8
36

Only first 15 entries are displayed. Click here to see all.

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68377]	D-glucose ChEBI	+	builds acid from	* from API NH
[Ref.: #68377]	maltose ChEBI	-	builds acid from	* from API NH
[Ref.: #120111]	nitrate ChEBI	+	reduction
[Ref.: #120111]	nitrite ChEBI	-	reduction
[Ref.: #68377]	ornithine ChEBI	-	degradation	* from API NH
[Ref.: #68377]	tryptophan ChEBI	+	energy source	* from API NH
[Ref.: #68377]	urea ChEBI	+	hydrolysis	* from API NH

	Metabolite production	Metabolite	Production
[Ref.: #120111]		indole ChEBI	yes
[Ref.: #68377]		indole ChEBI	yes	* from API NH

	Physiological tests	Metabolite	Indole test
[Ref.: #68377]		indole ChEBI	+	* from API NH

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120111]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68377]	alkaline phosphatase	+	3.1.3.1	* from API NH, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120111]	beta-galactosidase	-	3.2.1.23
[Ref.: #68377]	beta-galactosidase	-	3.2.1.23	* from API NH, API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68377]	beta-lactamase	-	3.5.2.6	* from API NH
[Ref.: #4562]	catalase	+	1.11.1.6
[Ref.: #120111]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #4562]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68377]	lipase	-		* from API NH
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #120111]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120111]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68377]	ornithine decarboxylase	-	4.1.1.17	* from API NH
[Ref.: #120111]	oxidase	-
[Ref.: #68377]	proline-arylamidase	-	3.4.11.5	* from API NH
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120111]	tryptophan deaminase	-
[Ref.: #68377]	tryptophan deaminase	+	4.1.99.1	* from API NH
[Ref.: #120111]	urease	+	3.5.1.5
[Ref.: #68377]	urease	+	3.5.1.5	* from API NH
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #49650]

Fatty acid	Percentage	ECL
C16:1 ω7c	37	15.819
C16:0	32	16
C14:0	19.3	14
C14:0 3OH/C16:1 ISO I	7.4	15.485
C18:0	1	18
C18:2 ω6,9c/C18:0 ANTE	0.7	17.724
C16:1 ω7c DMA	0.4	16.288
C15:0 ISO 3OH	0.4	16.135
unknown 14.503	0.4	14.503
C13:0 ISO 2OH	0.4	13.814
C18:1 ω7c /12t/9t	0.3	17.824
C16:1 ω5c	0.2	15.908
C12:0	0.2	12
C18:1 ω9c	0.1	17.769

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #4562]	6259	-	+	+	-	-	-	+	-	+	-	-	+	+
[Ref.: #49650]	1362	-	+	+	-	-	-	+	-	+	-	-	-	+
[Ref.: #49650]	2071	-	+	-	-	-	-	+	-	+	-	-	-	+
[Ref.: #4562]	5945	-	+	+	-	-	-	+	-	+	-	-	-	+
[Ref.: #4562]	5913	-	+	+	-	+	-	+	-	+	-	-	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #49650]	2327	-	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-
[Ref.: #120111]	7583	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4562]	Sample type/isolated from	lung abcess
[Ref.: #4562]	Country	USA
[Ref.: #4562]	Country ISO 3 Code	USA
[Ref.: #4562]	Continent	North America

[Ref.: #49650]	Sample type/isolated from	Human lung abscess,57-year-old patient

[Ref.: #120111]	Country	United States of America
[Ref.: #120111]	Country ISO 3 Code	USA
[Ref.: #120111]	Continent	North America
[Ref.: #120111]	Isolation date	1986
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Oral cavity and airways	#Lung
#Host Body-Site	#Other	#Abscess

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HE978269 (>99% sequence identity) for Haemophilus influenzae from Microbeatlas

Aquatic Samples	597
Soil Samples	173
Animal Samples	30711
Plant Samples	55
Total Samples	31536

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4562]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120111]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4562]	Haemophilus influenzae partial 16S rRNA gene, strain DSM 11970	HE978269	1532	GenBank ENA	727 tax ID

Availability in culture collections External links

[Ref.: #4562]

Culture collection no.

CCUG 29539, DSM 11970, ATCC 49766, CIP 103570, CCM 4457, NCTC 12975

[Ref.: #80923]

SI-ID 7367

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Enzymology	Overexpression and Purification of C-terminal Fragment of the Passenger Domain of Hap Protein from Nontypeable Haemophilus influenzae in a Highly Optimized Escherichia coli Expression System.	Tabatabaee A, Siadat SD, Moosavi SF, Aghasadeghi MR, Memarnejadian A, Pouriayevali MH, Yavari N	Avicenna J Med Biotechnol		2013	*
Pathogenicity	Quality control guidelines for cefdinir, cefepime, cefetamet, cefmetazole, cefpodoxime, cefprozil, and clinafloxacin (CI-960) for various National Committee for Clinical Laboratory Standards susceptibility testing methods. Quality Control Study Group.	Bale MJ, Jones RN	J Clin Microbiol	10.1128/jcm.31.9.2538-2540.1993	1993	*
Pathogenicity	Haemophilus influenzae ATCC 49766, an alternative quality control strain for monitoring broth microdilution susceptibility tests with selected beta-lactams.	Barry AL, Jorgensen JH, Hardy DJ, Allen SD, Baker CN	J Clin Microbiol	10.1128/jcm.30.8.2033-2037.1992	1992	*
Pathogenicity	Development of revised quality control limits for disk diffusion susceptibility tests of selected cephem antibiotics with Haemophilus influenzae and description of a new control strain.	Jorgensen JH, Barry AL, Doern GV, Ferraro MJ, Murray PR	J Clin Microbiol	10.1128/jcm.30.8.2029-2032.1992	1992	*
Pathogenicity	Ceftaroline activity on certain respiratory tract and wound infection agents at the minimum inhibitory concentration level.	Yanik K, Guluzade E, Bilgin K, Karadag A, Eroglu C, Birinci A, Gunaydin M	J Infect Dev Ctries	10.3855/jidc.6300	2015	*
	Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate.	Behrouzi A, Mianroodi RA, Afrough P, Ayadi A, Serajian A	Iran J Microbiol	10.18502/ijm.v12i5.4602	2020	*
	Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae.	Tavakoli M, Bouzari S, Siadat SD, Najar Peerayeh S, Jafari A	Jundishapur J Microbiol	10.5812/jjm.8(4)2015.16377	2015	*

References

#4562

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11970

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41776 ; Curators of the CIP;
#49650 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 29539

#68377

Automatically annotated from API NH .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#80923

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7367.1 )

#120111 Collection of Institut Pasteur ; Curators of the CIP; CIP 103570
* These data were automatically processed and therefore are not curated

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