Strain identifier

BacDive ID: 11703

Type strain: No

Species: Haemophilus influenzae

Strain Designation: L-378

Strain history: CIP <- 1992, J.H. Jorgensen, Univ. Texas Hlth. Sci. Center, San-Antonio, Texas, USA <- 1986, S. Farmer, Med. College Wisconsin, USA

NCBI tax ID(s): 727 (species)

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General

@ref: 4562

BacDive-ID: 11703

DSM-Number: 11970

keywords: 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Haemophilus influenzae L-378 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from lung abcess.

NCBI tax id

  • NCBI tax id: 727
  • Matching level: species

strain history

@refhistory
4562<- Heinrich Mack Nachf.; ATCC 49766 <- ATCC; <- J. H. Jorgensen; L-378 <- S. Farmer;
120111CIP <- 1992, J.H. Jorgensen, Univ. Texas Hlth. Sci. Center, San-Antonio, Texas, USA <- 1986, S. Farmer, Med. College Wisconsin, USA

doi: 10.13145/bacdive11703.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Haemophilus
  • species: Haemophilus influenzae
  • full scientific name: Haemophilus influenzae corrig. (Lehmann and Neumann 1896) Winslow et al. 1917 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Hemophilus influenzae
    20215Bacterium influenzae

@ref: 4562

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Haemophilus

species: Haemophilus influenzae

full scientific name: Haemophilus influenzae (Lehmann and Neumann 1896) Winslow et al. 1917

strain designation: L-378

type strain: no

Morphology

cell morphology

  • @ref: 120111
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation period
45621-2 days
120111

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4562HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
41776MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
120111CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10
120111CIP Medium 10_BIORADyeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10_BIORAD

culture temp

@refgrowthtypetemperaturerange
4562positivegrowth37mesophilic
41776positivegrowth37mesophilic
120111positivegrowth37mesophilic
120111nogrowth30mesophilic
120111nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
4562microaerophile
120111facultative anaerobe

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
4562M 804371microaerophil30-32832-344040-4232304440-423234-3640222481414-1634-3632-3420243862026-2818304420>5034-3624018642
4562M 80437microaerophil3063242-4440322844-4642303840-4224-26261018-20184040202434-360203022324418-20>503824020-22644
4562M 804301030-324040323042-44403236-38402424-26014-1614-163434-3620-222640-4202024-26203246-4816>5034-36181218-20040-42
4562M 80437microaerophil28826-2838343022-244038-402828-3034-3620200121032-34321820-22260162216-182440-421646-4830-3218014-166-836
4562M 80437microaerophil28826-2838343022-244038-402828-3034-3620200121032-34321820-22260162216-182440-421646-4830-3218014-166-836

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12011117632nitrate+reduction
12011116301nitrite-reduction
6837717634D-glucose+builds acid from
6837717306maltose-builds acid from
6837718257ornithine-degradation
6837716199urea+hydrolysis
6837727897tryptophan+energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleyes
12011135581indoleyes

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
4562catalase+1.11.1.6
4562cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase+4.1.99.1
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase+3.1.3.1
68377lipase-
68377urease+3.5.1.5
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
120111oxidase-
120111beta-galactosidase-3.2.1.23
120111alcohol dehydrogenase-1.1.1.1
120111catalase+1.11.1.6
120111lysine decarboxylase-4.1.1.18
120111ornithine decarboxylase-4.1.1.17
120111tryptophan deaminase-
120111urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    49650C12:00.212
    49650C14:019.314
    49650C16:03216
    49650C18:0118
    49650C13:0 ISO 2OH0.413.814
    49650C14:0 3OH/C16:1 ISO I7.415.485
    49650C15:0 ISO 3OH0.416.135
    49650C16:1 ω5c0.215.908
    49650C16:1 ω7c3715.819
    49650C16:1 ω7c DMA0.416.288
    49650C18:1 ω7c /12t/9t0.317.824
    49650C18:1 ω9c0.117.769
    49650C18:2 ω6,9c/C18:0 ANTE0.717.724
    49650unknown 14.5030.414.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
49650-+---+----++--------
120111-+++-+----++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
4562-++---+-+--++
49650-++---+-+---+
49650-+----+-+---+
4562-++---+-+---+
4562-++-+-+-+--++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
4562lung abcessUSAUSANorth America
49650Human lung abscess,57-year-old patient
120111United States of AmericaUSANorth America1986

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Oral cavity and airways#Lung
#Host Body-Site#Other#Abscess

taxonmaps

  • @ref: 69479
  • File name: preview.99_36.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_18;96_30;97_31;98_34;99_36&stattab=map
  • Last taxonomy: Haemophilus influenzae
  • 16S sequence: HE978269
  • Sequence Identity:
  • Total samples: 31536
  • soil counts: 173
  • aquatic counts: 597
  • animal counts: 30711
  • plant counts: 55

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
45622Risk group (German classification)
1201112Risk group (French classification)

Sequence information

16S sequences

  • @ref: 4562
  • description: Haemophilus influenzae partial 16S rRNA gene, strain DSM 11970
  • accession: HE978269
  • length: 1532
  • database: ena
  • NCBI tax ID: 727

External links

@ref: 4562

culture collection no.: CCUG 29539, DSM 11970, ATCC 49766, CIP 103570, CCM 4457, NCTC 12975

straininfo link

  • @ref: 80923
  • straininfo: 7367

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity1500510Development of revised quality control limits for disk diffusion susceptibility tests of selected cephem antibiotics with Haemophilus influenzae and description of a new control strain.Jorgensen JH, Barry AL, Doern GV, Ferraro MJ, Murray PRJ Clin Microbiol10.1128/jcm.30.8.2029-2032.19921992Ampicillin/pharmacology, Cefaclor/pharmacology, Cefamandole/pharmacology, Cefonicid/pharmacology, Cefuroxime/pharmacology, Cephalosporins/pharmacology, Evaluation Studies as Topic, Haemophilus influenzae/*drug effects/isolation & purification, Humans, Microbial Sensitivity Tests/methods/*standards, Quality Control, Reference StandardsEnzymology
Pathogenicity1500511Haemophilus influenzae ATCC 49766, an alternative quality control strain for monitoring broth microdilution susceptibility tests with selected beta-lactams.Barry AL, Jorgensen JH, Hardy DJ, Allen SD, Baker CNJ Clin Microbiol10.1128/jcm.30.8.2033-2037.19921992Ampicillin/pharmacology, Anti-Bacterial Agents/*pharmacology, Culture Media, Drug Resistance, Microbial, Evaluation Studies as Topic, Haemophilus influenzae/*drug effects, Humans, Microbial Sensitivity Tests/methods/*standards, Penicillin Resistance, Quality Control, Species SpecificityCultivation
Pathogenicity8408586Quality control guidelines for cefdinir, cefepime, cefetamet, cefmetazole, cefpodoxime, cefprozil, and clinafloxacin (CI-960) for various National Committee for Clinical Laboratory Standards susceptibility testing methods. Quality Control Study Group.Bale MJ, Jones RNJ Clin Microbiol10.1128/jcm.31.9.2538-2540.19931993Anti-Infective Agents/*pharmacology, Cefdinir, Cefepime, Cefmetazole/pharmacology, Ceftizoxime/analogs & derivatives/pharmacology, Cephalosporins/*pharmacology, *Fluoroquinolones, Humans, Microbial Sensitivity Tests/*standards, Quality Control, Quinolones/*pharmacology
Enzymology23919121Overexpression and Purification of C-terminal Fragment of the Passenger Domain of Hap Protein from Nontypeable Haemophilus influenzae in a Highly Optimized Escherichia coli Expression System.Tabatabaee A, Siadat SD, Moosavi SF, Aghasadeghi MR, Memarnejadian A, Pouriayevali MH, Yavari NAvicenna J Med Biotechnol2013
26034537Prokaryotic High-Level Expression System in Producing Adhesin Recombinant Protein E of Nontypeable Haemophilus influenzae.Tavakoli M, Bouzari S, Siadat SD, Najar Peerayeh S, Jafari AJundishapur J Microbiol10.5812/jjm.8(4)2015.163772015
Pathogenicity26517483Ceftaroline activity on certain respiratory tract and wound infection agents at the minimum inhibitory concentration level.Yanik K, Guluzade E, Bilgin K, Karadag A, Eroglu C, Birinci A, Gunaydin MJ Infect Dev Ctries10.3855/jidc.63002015Anti-Bacterial Agents/*pharmacology, Bacterial Infections/microbiology, Cephalosporins/*pharmacology, Haemophilus influenzae/*drug effects/isolation & purification, Humans, Microbial Sensitivity Tests, Moraxella catarrhalis/*drug effects/isolation & purification, Respiratory Tract Infections/*microbiology, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Streptococcus/*drug effects/isolation & purification, Wound Infection/*microbiologyEnzymology
33603996Evaluation of immunological responses against outer membrane vesicles (OMV) of nontypeable Haemophilus influenzae using MPLA-CpG adjuvant as a vaccine candidate.Behrouzi A, Mianroodi RA, Afrough P, Ayadi A, Serajian AIran J Microbiol10.18502/ijm.v12i5.46022020

Reference

@idauthorscataloguedoi/urltitle
4562Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11970)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11970
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
41776Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15382
49650Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 29539)https://www.ccug.se/strain?id=29539
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
80923Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7367.1StrainInfo: A central database for resolving microbial strain identifiers
120111Curators of the CIPCollection of Institut Pasteur (CIP 103570)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103570