Strain identifier

BacDive ID: 11704

Type strain: No

Species: Haemophilus influenzae

Strain Designation: Kilian HK 394, Pittman 576, 576, HK 394

Strain history: CIP <- 1954, M. Pittman, N.I.H.: strain 576 <- H.K. Killian strain HK 394

NCBI tax ID(s): 727 (species)

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General

@ref: 16829

BacDive-ID: 11704

DSM-Number: 23393

keywords: genome sequence, Bacteria, microaerophile, Gram-negative, rod-shaped

description: Haemophilus influenzae Kilian HK 394 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from human cerebrospinal fluid.

NCBI tax id

  • NCBI tax id: 727
  • Matching level: species

strain history

@refhistory
16829<- CCUG <- R. J. Owen, NCTC <- M. Pittman, NIH; Pittman 576
120108CIP <- 1954, M. Pittman, N.I.H.: strain 576 <- H.K. Killian strain HK 394

doi: 10.13145/bacdive11704.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pasteurellales
  • family: Pasteurellaceae
  • genus: Haemophilus
  • species: Haemophilus influenzae
  • full scientific name: Haemophilus influenzae corrig. (Lehmann and Neumann 1896) Winslow et al. 1917 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Hemophilus influenzae
    20215Bacterium influenzae

@ref: 16829

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pasteurellales

family: Pasteurellaceae

genus: Haemophilus

species: Haemophilus influenzae

full scientific name: Haemophilus influenzae (Lehmann and Neumann 1896) Winslow et al. 1917

strain designation: Kilian HK 394, Pittman 576, 576, HK 394

type strain: no

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
120108negativerod-shapedno
69480no95.06
69480negative96.915

colony morphology

  • @ref: 16829
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16829HAEMOPHILUS MEDIUM (DSMZ Medium 804)yeshttps://mediadive.dsmz.de/medium/804Name: HAEMOPHILUS MEDIUM (DSMZ Medium 804) Composition: Mueller-Hinton broth 21.0 g/l Yeast extract 5.0 g/l Distilled water
36559MEDIUM 10 - Chocolate medium for Actinobacillus pleuropneumoniae, Capnocytophaga cynodegmi, Haemophilus and NeisseriayesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml);PolyVitex mischung (10.000 ml)
120108CIP Medium 10yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=10

culture temp

@refgrowthtypetemperature
16829positivegrowth37
36559positivegrowth37

Physiology and metabolism

oxygen tolerance

  • @ref: 16829
  • oxygen tolerance: microaerophile

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
16829Mueller-Hinton Agar371aerob + 5% CO23003442383028-3046423640422224622-2412404020263202026203244204836-3822820838
16829M 804371microaerophil3003440383028-3046-48423840-425022-2424-2682014-164040202840-42020302236-3846-4826>504026022840
16829M 80430-32036-3844-4636-3830304844-4636-384048-50222410-1220-2214-164240-4220-2228361022261840503046-484018-208201040
16829M 80437microaerophil20020-2230242012323222-242832-34181861283030142020-22016201624-163210-12282812016624
16829M 80437microaerophil20020-2230242012323222-242832-34181861283030142020-22016201624-163210-12282812016624

spore formation

@refspore formationconfidence
69480no91.739
69481no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837717634D-glucose+builds acid from
6837727897tryptophan-energy source

metabolite production

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
16829catalase+1.11.1.6
16829cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    65609C14:015.914
    65609C16:03616
    65609C18:02.218
    65609C12:0 ALDE ?210.915
    65609C13:0 ISO 2OH0.313.814
    65609C14:0 3OH/C16:1 ISO I5.415.485
    65609C16:1 ω7c32.315.819
    65609C16:1 ω9c0.215.774
    65609C18:1 ω7c /12t/9t0.617.824
    65609C18:1 ω9c1.517.769
    65609C18:2 ω6,9c/C18:0 ANTE2.417.724
    65609Unidentified0.213.934
    65609unknown 14.503114.503
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
65609-++--+----++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
65609-++--++-+----
16829+++/-+/-++-+-----
16829++++++-+-----
16829++--++-+-----
16829+++/-+/--+-+-----
16829-++-+/-++-+----
16829-++++++-+----
16829-+++/-+/-++-+----
16829-++--++-+----
16829-+++/-+/--+-+----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
16829human cerebrospinal fluidUSAUSANorth America
120108Cerebrospinal fluidUnited States of AmericaUSANorth America1940

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Fluids#Cerebrospinal fluid
#Infection#Patient

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
168292Risk group (German classification)
1201082Risk group (French classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haemophilus influenzae NCTC8468GCA_901472485completencbi727
66792Haemophilus influenzae strain NCTC8468727.2184completepatric727

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno100no
69480gram-positivegram-positivePositive reaction to Gram-stainingno96.915no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no97.487yes
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no91.262yes
69480spore-formingspore-formingAbility to form endo- or exosporesno91.739no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno95.06no

External links

@ref: 16829

culture collection no.: DSM 23393, ATCC 9334, CCUG 13225B, CCUG 23946, CIP 54.94, NCTC 8468

straininfo link

  • @ref: 80924
  • straininfo: 54431

literature

  • topic: Pathogenicity
  • Pubmed-ID: 35109894
  • title: An invasive Haemophilus influenzae serotype b infection in an Anglo-Saxon plague victim.
  • authors: Guellil M, Keller M, Dittmar JM, Inskip SA, Cessford C, Solnik A, Kivisild T, Metspalu M, Robb JE, Scheib CL
  • journal: Genome Biol
  • DOI: 10.1186/s13059-021-02580-z
  • year: 2022
  • mesh: Child, *Haemophilus Vaccines, Haemophilus influenzae/genetics, Humans, Infant, Phylogeny, *Plague, Serogroup
  • topic2: Phylogeny

Reference

@idauthorscataloguedoi/urltitle
16829Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23393)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23393
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36559Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18411
65609Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 23946)https://www.ccug.se/strain?id=23946
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
80924Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID54431.1StrainInfo: A central database for resolving microbial strain identifiers
120108Curators of the CIPCollection of Institut Pasteur (CIP 54.94)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2054.94