Name: Legionella pneumophila subsp. pneumophila Benson 1169-MN-H
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6725

Type strain:

Species: Legionella pneumophila subsp. pneumophila

Strain Designation: Benson 1169-MN-H, R69, 1169-MN-H SG 14, 1169-MN-H

Culture col. no.: DSM 25226, ATCC 43703, CCUG 30663, CIP 103869, NCTC 12174

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.F. Benson: strain 1169-MN-H <- Minnesota Dept. Hlth. <- J.A. Washington

NCBI tax ID(s): 91891 (subspecies)

SI-ID 41996

ATCC 43703, CCUG 30663, CCUG 30709, NCTC 12174, CIP 103869, CCUG 44898

Other strains from Legionella pneumophila subsp. pneumophila

Section

Legionella pneumophila subsp. pneumophila Benson 1169-MN-H is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from bronchial aspirate.

Gram-negative
mesophilic
microaerophile
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella pneumophila subsp. pneumophila**
	Full Scientific Name (LPSN)	Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

Information on morphological and physiological properties Morphology

[Ref.: #121911]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #121911]	Cell shape	rod-shaped

[Ref.: #121911]	Motility	no

[Ref.: #17947]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17947]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #17947]

Culture medium growth

[Ref.: #17947]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17947]

Culture medium composition

expand / minimize

[Ref.: #38484]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #38484]

Culture medium growth

[Ref.: #38484]

Culture medium composition

expand / minimize

[Ref.: #121911]

Culture medium

CIP Medium 23

[Ref.: #121911]

Culture medium growth

[Ref.: #121911]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #17947]			growth	37 °C
[Ref.: #38484]			growth	37 °C

[Ref.: #17947]	Temperature range	mesophilic
[Ref.: #38484]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #17947]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.991

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #17947]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #17947]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121911]	7716	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17947]	Sample type/isolated from	bronchial aspirate
[Ref.: #17947]	Geographic location (country and/or sea, region)	Minnesota
[Ref.: #17947]	Country	USA
[Ref.: #17947]	Country ISO 3 Code	USA
[Ref.: #17947]	Continent	North America

[Ref.: #121911]	Sample type/isolated from	Human, Bronchial aspirate
[Ref.: #121911]	Geographic location (country and/or sea, region)	Minnesota
[Ref.: #121911]	Country	United States of America
[Ref.: #121911]	Country ISO 3 Code	USA
[Ref.: #121911]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host Body Product	#Fluids	#Aspirate
#Host Body-Site	#Oral cavity and airways	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17947]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121911]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella pneumophila NCTC12174	GCA_900461535	contig	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila subsp. pneumophila ATCC 43703	GCA_000586095	scaffold	GenBank	91891 tax ID	*
[Ref.: #66792]	Legionella pneumophila strain NCTC12174	446.732	wgs	PATRIC	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila subsp. pneumophila strain ATCC 43703	91891.25	wgs	PATRIC	91891 tax ID	*
[Ref.: #66792]	Legionella pneumophila pneumophila ATCC 43703	2657245690	draft	JGI IMG/M	91891 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Legionella pneumophila subsp. pneumophila strain ATCC 43703 PATRIC: 91891.25
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella pneumophila subsp. pneumophila ATCC 43703 NCBI: GCA_000586095.1
[Ref.: #69480]	motile	yes	79.87	no
[Ref.: #69480]	flagellated	no	73.003	no
[Ref.: #69480]	gram-positive	no	98.736	no
[Ref.: #69480]	anaerobic	no	98.63	no
[Ref.: #69480]	halophile	no	86.427	no
[Ref.: #69480]	spore-forming	no	94.895	no
[Ref.: #69480]	glucose-util	yes	68.714	no
[Ref.: #69480]	aerobic	no	92.12	yes
[Ref.: #69480]	thermophile	no	96.786	yes
[Ref.: #69480]	glucose-ferment	no	86.3	no

Availability in culture collections External links

[Ref.: #17947]

Culture collection no.

DSM 25226, ATCC 43703, CCUG 30663, CIP 103869, NCTC 12174

[Ref.: #76195]

SI-ID 41996

References

#17947

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25226

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#38484 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76195

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41996.1 )

#121911 Collection of Institut Pasteur ; Curators of the CIP; CIP 103869
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Legionella pneumophila subsp. pneumophila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Legionella pneumophila subsp. pneumophila strain ATCC 43703

Legionella pneumophila subsp. pneumophila ATCC 43703

Change proposal

Change proposal