Name: Legionella pneumophila subsp. pneumophila R63
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6784

Type strain:

Species: Legionella pneumophila subsp. pneumophila

Strain Designation: R63, OLDA SG1, OLDA

Culture col. no.: DSM 25200, ATCC 43109, CIP 103855, NCTC 12008

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- J.R. Joly: strain OLDA

NCBI tax ID(s): 91891 (subspecies)

SI-ID 41654

ATCC 43109, NCTC 12008, CIP 103855

Other strains from Legionella pneumophila subsp. pneumophila

Section

Legionella pneumophila subsp. pneumophila R63 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from Human.

Gram-negative
mesophilic
microaerophile
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella pneumophila subsp. pneumophila**
	Full Scientific Name (LPSN)	Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

Information on morphological and physiological properties Morphology

[Ref.: #121899]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #121899]	Cell shape	rod-shaped

[Ref.: #121899]	Motility	no

[Ref.: #17937]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17937]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #17937]

Culture medium growth

[Ref.: #17937]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17937]

Culture medium composition

expand / minimize

[Ref.: #40145]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40145]

Culture medium growth

[Ref.: #40145]

Culture medium composition

expand / minimize

[Ref.: #121899]

Culture medium

CIP Medium 23

[Ref.: #121899]

Culture medium growth

[Ref.: #121899]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #17937]			growth	37 °C
[Ref.: #40145]			growth	37 °C

[Ref.: #17937]	Temperature range	mesophilic
[Ref.: #40145]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #17937]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.99

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #17937]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121899]	7703	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #121899]	Sample type/isolated from	Human
[Ref.: #121899]	Country	Republic of Korea
[Ref.: #121899]	Country ISO 3 Code	KOR
[Ref.: #121899]	Continent	Asia
[Ref.: #121899]	Isolation date	1993
* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17937]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121899]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella pneumophila NCTC12008	GCA_900452705	contig	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila strain NCTC12008	446.726	wgs	PATRIC	446 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Legionella pneumophila strain NCTC12008 PATRIC: 446.726
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella pneumophila strain NCTC12008 PATRIC: 446.726
[Ref.: #69480]	gram-positive	no	98.424	no
[Ref.: #69480]	anaerobic	no	98.642	yes
[Ref.: #69480]	halophile	no	86.236	no
[Ref.: #69480]	spore-forming	no	94.722	no
[Ref.: #69480]	glucose-util	yes	68.484	no
[Ref.: #69480]	aerobic	no	91.734	yes
[Ref.: #69480]	flagellated	no	79.297	no
[Ref.: #69480]	thermophile	no	98.082	no
[Ref.: #69480]	motile	yes	77.407	no
[Ref.: #69480]	glucose-ferment	no	87.969	no

Availability in culture collections External links

[Ref.: #17937]

Culture collection no.

DSM 25200, ATCC 43109, CIP 103855, NCTC 12008

[Ref.: #76254]

SI-ID 41654

References

#17937

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25200

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40145 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76254

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID41654.1 )

#121899 Collection of Institut Pasteur ; Curators of the CIP; CIP 103855
* These data were automatically processed and therefore are not curated

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Legionella pneumophila strain NCTC12008

Legionella pneumophila strain NCTC12008

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