Name: Legionella pneumophila subsp. pneumophila R51
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6721

Type strain:

Species: Legionella pneumophila subsp. pneumophila

Strain Designation: R51, Chicago 2 SG 6, Chicago 2

Culture col. no.: DSM 25182, ATCC 33215, CIP 103860, NCTC 11406

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.M. McKinney: strain Chicago 2 <- H. Sommers

NCBI tax ID(s): 933085 (strain), 91891 (subspecies)

SI-ID 40395

ATCC 33215, CIP 103860, NCTC 11406

Other strains from Legionella pneumophila subsp. pneumophila

Section

Legionella pneumophila subsp. pneumophila R51 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from human lung biopsy.

Gram-negative
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella pneumophila subsp. pneumophila**
	Full Scientific Name (LPSN)	Legionella pneumophila subsp. pneumophila (Brenner et al. 1979) Brenner et al. 1989

Information on morphological and physiological properties Morphology

[Ref.: #121912]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.999

[Ref.: #121912]	Cell shape	rod-shaped

[Ref.: #121912]	Motility	no

[Ref.: #17927]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #17927]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #17927]

Culture medium growth

[Ref.: #17927]

Culture medium link

Medium recipe at MediaDive

[Ref.: #17927]

Culture medium composition

expand / minimize

[Ref.: #38479]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #38479]

Culture medium growth

[Ref.: #38479]

Culture medium composition

expand / minimize

[Ref.: #121912]

Culture medium

CIP Medium 23

[Ref.: #121912]

Culture medium growth

[Ref.: #121912]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #17927]			growth	37 °C
[Ref.: #38479]			growth	37 °C

[Ref.: #17927]	Temperature range	mesophilic
[Ref.: #38479]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #17927]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.977

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #17927]	catalase	+	1.11.1.6
[Ref.: #121912]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #17927]	cytochrome-c oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121912]	oxidase	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121912]	7708	-	+	+	+	-	+	+	+	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #17927]	Sample type/isolated from	human lung biopsy

[Ref.: #121912]	Sample type/isolated from	Human, Lung biopsy
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Biopsy
#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Lung

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #17927]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121912]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	L.pneumophila sgp6 (ATCC 33215) gene for 16S rRNA	X73402	1467	ENA	933085 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Chicago-2) 16S rRNA, partial, segment 2 of 3	M19442	131	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Chicago-2) 16S rRNA, partial, segment 1 of 3	M19448	123	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Chicago-2) 16S rRNA, partial, segment 3 of 3	M22264	116	ENA	446 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella pneumophila subsp. pneumophila ATCC 33215	GCA_000586295	scaffold	GenBank	933085 tax ID	*
[Ref.: #66792]	Legionella pneumophila subsp. pneumophila ATCC 33215	933085.3	wgs	PATRIC	933085 tax ID	*
[Ref.: #66792]	Legionella pneumophila pneumophila ATCC 33215	2667527927	draft	JGI IMG/M	933085 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Legionella pneumophila subsp. pneumophila ATCC 33215 ATCC 33215 NCBI: GCA_000586295.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella pneumophila subsp. pneumophila ATCC 33215 ATCC 33215 NCBI: GCA_000586295.1
[Ref.: #69480]	motile	yes	80.93	no
[Ref.: #69480]	flagellated	no	69.377	no
[Ref.: #69480]	gram-positive	no	98.668	no
[Ref.: #69480]	anaerobic	no	98.562	yes
[Ref.: #69480]	halophile	no	87.038	no
[Ref.: #69480]	spore-forming	no	94.629	no
[Ref.: #69480]	glucose-util	yes	69.457	no
[Ref.: #69480]	aerobic	no	92.401	yes
[Ref.: #69480]	thermophile	no	97.776	no
[Ref.: #69480]	glucose-ferment	no	85.705	no

Availability in culture collections External links

[Ref.: #17927]

Culture collection no.

DSM 25182, ATCC 33215, CIP 103860, NCTC 11406

[Ref.: #76191]

SI-ID 40395

References

#17927

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25182

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38479 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76191

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40395.1 )

#121912 Collection of Institut Pasteur ; Curators of the CIP; CIP 103860
* These data were automatically processed and therefore are not curated

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Legionella pneumophila subsp. pneumophila ATCC 33215 ATCC 33215

Legionella pneumophila subsp. pneumophila ATCC 33215 ATCC 33215

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