Legionella pneumophila Philadelphia-1 | Type strain | DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

Strain identifier

BacDive ID: 6712

Type strain:

Species: Legionella pneumophila

Strain Designation: Philadelphia-1

Culture col. no.: DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	31-03-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Proteobacteria
	Class	Gammaproteobacteria
	Order	Legionellales
	Family	Legionellaceae
	Genus	Legionella
	Species	Legionella pneumophila
	Full Scientific Name (PNU)	Legionella pneumophila Brenner et al. 1979 (Approved Lists 1980)

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #3193]

Incubation period

2-3 days

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #3193]		catalase	+	1.11.1.6
[Ref.: #3193]		cytochrome-c oxidase	+	1.9.3.1

Fatty acid profile

Type of FA analysis

whole cell analysis

Incubation medium

Agar/liquid

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Library/peak naming table

System

Instrument

Method/protocol

CCUG

Cutoff value

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
15:0	0.6	15
16:0	12	16
17:0	0.9	17
18:0	3	18
20:0	1.9	20
14:0 ISO	2.4	13.618
14:0 ISO 3OH	0.8	15.117
14:1 ω5c	0.7	13.899
15:0 ANTEISO	9.4	14.711
15:1 ω6c	1.5	14.856
16:0 ISO	23.4	15.626
16:1 ISO H	4.9	15.461
16:1 ω7c	29.6	15.819
17:0 ANTEISO	7.4	16.722
17:1 ω9c ANTEISO	1.4	16.525

Type of FA analysis

whole cell analysis

Incubation medium

Agar/liquid

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Library/peak naming table

System

Instrument

Method/protocol

CCUG

Cutoff value

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
14:0	1.5	14
15:0	1.1	15
16:0	9.8	16
17:0	0.7	17
18:0	2.3	18
19:0	0.5	19
20:0	2.2	20
14:0 ISO	5	13.618
14:0 ISO 3OH	1.3	15.117
14:1 ω5c	1.8	13.899
15:0 ANTEISO	9.2	14.711
15:1 ω6c	3.3	14.856
16:0 ISO	17.8	15.626
16:1 ISO H	2.7	15.461
16:1 ω7c	35.3	15.819
17:0 ANTEISO	3.4	16.722
17:0 CYCLO	1.2	16.888
Unidentified	0.4	13.244
Unidentified	0.9	16.318

Type of FA analysis

whole cell analysis

Incubation medium

Agar/liquid

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Library/peak naming table

System

Instrument

Method/protocol

CCUG

Cutoff value

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
14:0	0.5	14
15:0	0.9	15
16:0	5.7	16
17:0	1	17
18:0	1.9	18
19:0	0.5	19
20:0	1.6	20
14:0 ISO	7	13.618
14:0 ISO 3OH	1	15.117
14:1 ω5c	1.3	13.899
15:0 ANTEISO	11	14.711
15:1 ω6c	3.8	14.856
16:0 ISO	26.5	15.626
16:1 ISO H	4.1	15.461
16:1 ω7c	26.9	15.819
17:0 ANTEISO	4.2	16.722
17:0 CYCLO	1.6	16.888
17:1 ω9c ANTEISO	0.5	16.525

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #3193]	2487	-	-	-	-	-	-	+/-	+	-	-	+	-	-
[Ref.: #3193]	2486	+	+	+	+	+/-	-	-	+	-	-	+	-	-
[Ref.: #3193]	2485	+	+/-	-	-	-	-	+	+	-	-	+	-	-

[Ref.: #3193]	Oxygen tolerance	microaerophile
[Ref.: #45204]	Oxygen tolerance	microaerophile

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #3193]

Culture medium

BCYE-AGAR (DSMZ Medium 585), 37°C, microaerophilic; needs high humidity

[Ref.: #3193]

Culture medium growth

[Ref.: #3193]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #40120]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40120]

Culture medium growth

[Ref.: #40120]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #3193]			growth	37 ̊C
[Ref.: #40120]			growth	37 ̊C

[Ref.: #3193]	Temperature range	mesophilic
[Ref.: #40120]	Temperature range	mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #3193]

Sample type/isolated from

human lung tissue

[Ref.: #3193]

Geographic location (country and/or sea, region)

Philadelphia

[Ref.: #3193]

Country

USA

[Ref.: #3193]

Continent

North America

[Ref.: #45204]

Sample type/isolated from

Human lung

[Ref.: #45204]

Geographic location (country and/or sea, region)

Philadelphia

[Ref.: #45204]

Country

USA

[Ref.: #45204]

Continent

North America

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Lung

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #3193]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #3193]

GC-content

39.0 mol%

thermal denaturation, midpoint method (Tm)

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #3193]	complete genome, GenBank Genome project data	Legionella pneumophila subsp. pneumophila str. Philadelphia 1, complete genome	AE017354 ENA	WGS	3397754	272624 tax ID
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Legionella pneumophila gyrB gene for DNA gyrase subunit B, partial cds, clone: gy10041.icb	AB014928 ENA		1167	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC33152 16S-23S rRNA intergenic region with tRNA-Ala-like sequence	AF000655 ENA	16S	448	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila serogroup 1 major outer membrane protein precursor (mompS) gene, complete cds	AF078136 ENA		1157	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 defect in organelle trafficking protein (dotA) gene, partial cds	AF095231 ENA		3105	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila subsp. pneumophila ATCC 33152 RNA polymerase B subunit (rpoB) gene, partial cds	AF367748 ENA		300	91891 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 IcmB (icmB) gene, partial cds	AF527122 ENA		240	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 DotB (dotB) gene, partial cds	AF527139 ENA		346	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 IcmT (icmT) gene, partial cds	AF527156 ENA		264	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila djlA and kdtA (waaA) genes	AJ011775 ENA		3545	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila lepB gene signal peptidase I, strain ATCC 33152	AJ608705 ENA		756	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila partial gyrB gene for DNA gyrase subunit B	AJ617789 ENA		253	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila partial rnpB gene for ribonuclease P RNA, type strain CCUG 9568T	AJ781429 ENA		325	91891 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial flaA gene for flagellin, strain ATCC 33152	AJ878789 ENA		247	272624 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial proA gene, strain ATCC 33152	AJ878810 ENA		472	272624 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial pilE gene	AJ878823 ENA		443	272624 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial asd gene	AJ878835 ENA		556	272624 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila partial rpoB gene for RNA polymerase B-subunit, strain ATCC 33152	AM931899 ENA		300	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 322 from Patent WO0123604	AX109589 ENA		824	91891 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 1772 from Patent WO0123604	AX111039 ENA		968	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 DotA (dotA) gene, partial cds	AY036018 ENA		360	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY298787 ENA	16S	3248	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain Philadelphia-1 heat shock protein 60 (hsp60) gene, complete cds	EU867419 ENA		1647	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain DSM 7513 gyrase B (gyrB) gene, partial cds	GQ426107 ENA		1187	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ST12800 macrophage infectivity potentiator (mip) gene, partial cds	GU083757 ENA		645	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ST12804 serogroup 1 ribonuclease P RNA (rnpB) gene, partial sequence	GU083816 ENA		354	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN1 VNTR marker Lpms1 genomic sequence	GU598121 ENA		566	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN2 VNTR marker Lpms3 genomic sequence	GU598122 ENA		875	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN3 VNTR marker Lpms33 genomic sequence	GU598123 ENA		227	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN4 VNTR marker Lpms35 genomic sequence	GU598124 ENA		202	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN5 VNTR marker Lpms13 genomic sequence	GU598125 ENA		427	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN6 VNTR marker Lpms17 genomic sequence	GU598126 ENA		276	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN7 VNTR marker Lpms19 genomic sequence	GU598127 ENA		173	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN8 VNTR marker Lpms34 genomic sequence	GU598128 ENA		209	446 tax ID	*
[Ref.: #20218]	INSDC Sequence	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 2 of 3	M19444 ENA	16S	137	446 tax ID	*
[Ref.: #20218]	INSDC Sequence	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 1 of 3	M19450 ENA	16S	125	446 tax ID	*
[Ref.: #20218]	INSDC Sequence	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 3 of 3	M22266 ENA	16S	141	446 tax ID	*
[Ref.: #20218]	INSDC Sequence	Legionella pneumophila 16S ribosomal RNA	M59157 ENA	16S	1544	446 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila coded portion of proteolysis tag tmRNA gene	U68079 ENA		407	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	L.pneumophila Sgp.1 (ATCC 33152) gene for 5S ribosomal RNA	X72354 ENA		64	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	L.pneumophila 23S and 5S rRNA genes, partial CDS's	Z24705 ENA		336	446 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	L.pneumophila Serogroup 1 Philadelphia-1 gene for 23S ribosomal RNA and 5S ribosomal RNA	Z30431 ENA		336	446 tax ID	*
[Ref.: #3193]	gene for mip, Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial mip gene, strain ATCC 33152	AJ878849 ENA		561	272624 tax ID

Availability in culture collectionsStrain availability

[Ref.: #3193]

Culture collection no.

DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

[Ref.: #3193]

Strain history

<- ATCC <- R.E. Weaver, strain Philadelphia-1

[Ref.: #20218]

Associated Passport(s) in StrainInfo

160885, 108916, 866305

References

#3193

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7513

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40120 ; Curators of the CIP;
#45204 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 9568
* These References are textmined

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