Legionella pneumophila Philadelphia-1 | Type strain | DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

Strain identifier

BacDive ID: 6712

Type strain:

Species: Legionella pneumophila

Strain Designation: Philadelphia-1

Culture col. no.: DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Isolation, sampling and environmental information
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #3193]	Domain	Bacteria
[Ref.: #3193]	Phylum	Proteobacteria
[Ref.: #3193]	Class	Gammaproteobacteria
[Ref.: #3193]	Order	Legionellales
[Ref.: #3193]	Family	Legionellaceae
[Ref.: #3193]	Genus	Legionella
[Ref.: #3193]	Species	Legionella pneumophila
[Ref.: #3193]	Full Scientific Name	Legionella pneumophila subsp. pneumophila Brenner et al. 1979
[Ref.: #3193]	Strain Designation	Philadelphia-1
[Ref.: #3193]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	19-11-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Proteobacteria
[Ref.: #20215]	Class	Gammaproteobacteria		Int. J. Syst. Evol. Microbiol. 55:2235
[Ref.: #20215]	Family	Legionellaceae		Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Genus	Legionella	genus (AL)	Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Species	Legionella pneumophila	species (AL)	Int. J. Syst. Bacteriol. 30:225
[Ref.: #20215]	Full Scientific Name	Legionella pneumophila Brenner et al. 1979

[Ref.: #20215]

Synonym

Legionella pneumophila subsp. pneumophila

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #3193]

Incubation period

2-3 days

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #3193]		catalase	+	1.11.1.6
[Ref.: #3193]		cytochrome-c oxidase	+	1.9.3.1

Fatty acid profile

Type of FA analysis

whole cell analysis

Incubation medium

Agar/liquid

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Library/peak naming table

System

Instrument

Method/protocol

CCUG

Cutoff value

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
15:0	0.6	15
16:0	12	16
17:0	0.9	17
18:0	3	18
20:0	1.9	20
14:0 ISO	2.4	13.618
14:0 ISO 3OH	0.8	15.117
14:1 ω5c	0.7	13.899
15:0 ANTEISO	9.4	14.711
15:1 ω6c	1.5	14.856
16:0 ISO	23.4	15.626
16:1 ISO H	4.9	15.461
16:1 ω7c	29.6	15.819
17:0 ANTEISO	7.4	16.722
17:1 ω9c ANTEISO	1.4	16.525

Type of FA analysis

whole cell analysis

Incubation medium

Agar/liquid

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Library/peak naming table

System

Instrument

Method/protocol

CCUG

Cutoff value

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
14:0	1.5	14
15:0	1.1	15
16:0	9.8	16
17:0	0.7	17
18:0	2.3	18
19:0	0.5	19
20:0	2.2	20
14:0 ISO	5	13.618
14:0 ISO 3OH	1.3	15.117
14:1 ω5c	1.8	13.899
15:0 ANTEISO	9.2	14.711
15:1 ω6c	3.3	14.856
16:0 ISO	17.8	15.626
16:1 ISO H	2.7	15.461
16:1 ω7c	35.3	15.819
17:0 ANTEISO	3.4	16.722
17:0 CYCLO	1.2	16.888
Unidentified	0.4	13.244
Unidentified	0.9	16.318

Type of FA analysis

whole cell analysis

Incubation medium

Agar/liquid

Incubation temperature (°C)

Incubation time (d)

Incubation pH

Incubation oxygen condition

Treatment/condition

Software version

Library/peak naming table

System

Instrument

Method/protocol

CCUG

Cutoff value

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
14:0	0.5	14
15:0	0.9	15
16:0	5.7	16
17:0	1	17
18:0	1.9	18
19:0	0.5	19
20:0	1.6	20
14:0 ISO	7	13.618
14:0 ISO 3OH	1	15.117
14:1 ω5c	1.3	13.899
15:0 ANTEISO	11	14.711
15:1 ω6c	3.8	14.856
16:0 ISO	26.5	15.626
16:1 ISO H	4.1	15.461
16:1 ω7c	26.9	15.819
17:0 ANTEISO	4.2	16.722
17:0 CYCLO	1.6	16.888
17:1 ω9c ANTEISO	0.5	16.525

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #3193]	2487	-	-	-	-	-	-	+/-	+	-	-	+	-	-
[Ref.: #3193]	2486	+	+	+	+	+/-	-	-	+	-	-	+	-	-
[Ref.: #3193]	2485	+	+/-	-	-	-	-	+	+	-	-	+	-	-

[Ref.: #3193]	Oxygen tolerance	microaerophile
[Ref.: #45204]	Oxygen tolerance	microaerophile

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #3193]

Culture medium

BCYE-AGAR (DSMZ Medium 585), 37°C, microaerophilic; needs high humidity

[Ref.: #3193]

Culture medium growth

[Ref.: #3193]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #40120]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40120]

Culture medium growth

[Ref.: #40120]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #3193]			growth	37 ̊C
[Ref.: #40120]			growth	37 ̊C

[Ref.: #3193]	Temperature range	mesophilic
[Ref.: #40120]	Temperature range	mesophilic

Information on isolation source, the sampling and environmental conditionsIsolation, sampling and environmental information

[Ref.: #3193]

Sample type/isolated from

human lung tissue

[Ref.: #3193]

Geographic location (country and/or sea, region)

Philadelphia

[Ref.: #3193]

Country

USA

[Ref.: #3193]

Continent

North America

[Ref.: #45204]

Sample type/isolated from

Human lung

[Ref.: #45204]

Geographic location (country and/or sea, region)

Philadelphia

[Ref.: #45204]

Country

USA

[Ref.: #45204]

Continent

North America

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Organ	#Lung

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #3193]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

[Ref.: #3193]

GC-content

39.0 mol%

thermal denaturation, midpoint method (Tm)

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #3193]	complete genome, GenBank Genome project data	Legionella pneumophila subsp. pneumophila str. Philadelphia 1, complete genome	AE017354		3397754	272624
[Ref.: #20218]	Marker Gene (DDBJ Direct submission)	Legionella pneumophila gyrB gene for DNA gyrase subunit B, partial cds, clone: gy10041.icb	AB014928		1167	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC33152 16S-23S rRNA intergenic region with tRNA-Ala-like sequence	AF000655	16S	448	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila serogroup 1 major outer membrane protein precursor (mompS) gene, complete cds	AF078136		1157	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 defect in organelle trafficking protein (dotA) gene, partial cds	AF095231		3105	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila subsp. pneumophila ATCC 33152 RNA polymerase B subunit (rpoB) gene, partial cds	AF367748		300	91891	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 IcmB (icmB) gene, partial cds	AF527122		240	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 DotB (dotB) gene, partial cds	AF527139		346	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 IcmT (icmT) gene, partial cds	AF527156		264	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila djlA and kdtA (waaA) genes	AJ011775		3545	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila lepB gene signal peptidase I, strain ATCC 33152	AJ608705		756	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila partial gyrB gene for DNA gyrase subunit B	AJ617789		253	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila partial rnpB gene for ribonuclease P RNA, type strain CCUG 9568T	AJ781429		325	91891	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial flaA gene for flagellin, strain ATCC 33152	AJ878789		247	272624	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial proA gene, strain ATCC 33152	AJ878810		472	272624	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial pilE gene	AJ878823		443	272624	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial asd gene	AJ878835		556	272624	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Legionella pneumophila partial rpoB gene for RNA polymerase B-subunit, strain ATCC 33152	AM931899		300	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 322 from Patent WO0123604	AX109589		824	91891	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 1772 from Patent WO0123604	AX111039		968	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 DotA (dotA) gene, partial cds	AY036018		360	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY298787	16S	3248	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain Philadelphia-1 heat shock protein 60 (hsp60) gene, complete cds	EU867419		1647	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain DSM 7513 gyrase B (gyrB) gene, partial cds	GQ426107		1187	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ST12800 macrophage infectivity potentiator (mip) gene, partial cds	GU083757		645	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ST12804 serogroup 1 ribonuclease P RNA (rnpB) gene, partial sequence	GU083816		354	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN1 VNTR marker Lpms1 genomic sequence	GU598121		566	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN2 VNTR marker Lpms3 genomic sequence	GU598122		875	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN3 VNTR marker Lpms33 genomic sequence	GU598123		227	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN4 VNTR marker Lpms35 genomic sequence	GU598124		202	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN5 VNTR marker Lpms13 genomic sequence	GU598125		427	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN6 VNTR marker Lpms17 genomic sequence	GU598126		276	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN7 VNTR marker Lpms19 genomic sequence	GU598127		173	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila strain ATCC 33152 isolate SSCP gel band BN8 VNTR marker Lpms34 genomic sequence	GU598128		209	446	*
[Ref.: #20218]	INSDC Sequence	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 2 of 3	M19444	16S	137	446	*
[Ref.: #20218]	INSDC Sequence	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 1 of 3	M19450	16S	125	446	*
[Ref.: #20218]	INSDC Sequence	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 3 of 3	M22266	16S	141	446	*
[Ref.: #20218]	INSDC Sequence	Legionella pneumophila 16S ribosomal RNA	M59157	16S	1544	446	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Legionella pneumophila coded portion of proteolysis tag tmRNA gene	U68079		407	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	L.pneumophila Sgp.1 (ATCC 33152) gene for 5S ribosomal RNA	X72354		64	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	L.pneumophila 23S and 5S rRNA genes, partial CDS's	Z24705		336	446	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	L.pneumophila Serogroup 1 Philadelphia-1 gene for 23S ribosomal RNA and 5S ribosomal RNA	Z30431		336	446	*
[Ref.: #3193]	gene for mip, Marker Gene (EMBL Direct submission)	Legionella pneumophila subsp. pneumophila str. Philadelphia 1 partial mip gene, strain ATCC 33152	AJ878849		561	272624

Availability in culture collectionsStrain availability

[Ref.: #3193]

Culture collection no.

DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

[Ref.: #3193]

Strain history

<- ATCC <- R.E. Weaver, strain Philadelphia-1

[Ref.: #20218]

Associated Passport(s) in StrainInfo

160885, 108916, 866305

References

#3193

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7513

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40120 ; Curators of the CIP;
#45204 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 9568
* These References are textmined

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Legionella pneumophila in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF