Name: Legionella pneumophila Philadelphia-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6712

Type strain:

Species: Legionella pneumophila

Strain Designation: Philadelphia-1

Culture col. no.: DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

Strain history: <- ATCC <- R.E. Weaver, strain Philadelphia-1

NCBI tax ID(s): 446 (species)

Other strains from Legionella pneumophila

Section

Legionella pneumophila Philadelphia-1 is a microaerophile, mesophilic bacterium that was isolated from human lung tissue.

mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	23-11-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Proteobacteria
	Class	Gammaproteobacteria
	Order	Legionellales
	Family	Legionellaceae
	Genus	Legionella
	Species	Legionella pneumophila
	Full Scientific Name (PNU)	Legionella pneumophila Brenner et al. 1979 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #3193]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3193]

Culture medium

BCYE-AGAR (DSMZ Medium 585), microaerophilic; needs high humidity

[Ref.: #3193]

Culture medium growth

[Ref.: #3193]

Culture medium link

Medium recipe at BacMedia

[Ref.: #3193]

Culture medium composition

expand / minimize

[Ref.: #40120]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40120]

Culture medium growth

[Ref.: #40120]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #3193]			growth	37 °C
[Ref.: #40120]			growth	37 °C

[Ref.: #3193]	Temperature range	mesophilic
[Ref.: #40120]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #3193]	Oxygen tolerance	microaerophile
[Ref.: #45204]	Oxygen tolerance	microaerophile

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #3193]		catalase	+	1.11.1.6
[Ref.: #3193]		cytochrome-c oxidase	+	1.9.3.1

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
C16:1 ω7c	29.6	15.819
C16:0 iso	23.4	15.626
C16:0	12	16
C15:0 ANTEISO	9.4	14.711
C17:0 anteiso	7.4	16.722
C16:1 ISO H	4.9	15.461
C18:0	3	18
C14:0 ISO	2.4	13.618
C20:0	1.9	20
C15:1 ω6c	1.5	14.856
C17:1 ω9c ANTEISO	1.4	16.525
C17:0	0.9	17
C14:0 ISO 3OH	0.8	15.117
C14:1 ω5c	0.7	13.899
C15:0	0.6	15

Metadata FA analysis

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
C16:1 ω7c	35.3	15.819
C16:0 iso	17.8	15.626
C16:0	9.8	16
C15:0 ANTEISO	9.2	14.711
C14:0 ISO	5	13.618
C17:0 anteiso	3.4	16.722
C15:1 ω6c	3.3	14.856
C16:1 ISO H	2.7	15.461
C18:0	2.3	18
C20:0	2.2	20
C14:1 ω5c	1.8	13.899
C14:0	1.5	14
C14:0 ISO 3OH	1.3	15.117
C17:0 CYCLO	1.2	16.888
C15:0	1.1	15
Unidentified	0.9	16.318
C17:0	0.7	17
C19:0	0.5	19
Unidentified	0.4	13.244

Metadata FA analysis

Reference

[Ref.: #45204]

Fatty acid	Percentage	ECL
C16:1 ω7c	26.9	15.819
C16:0 iso	26.5	15.626
C15:0 ANTEISO	11	14.711
C14:0 ISO	7	13.618
C16:0	5.7	16
C17:0 anteiso	4.2	16.722
C16:1 ISO H	4.1	15.461
C15:1 ω6c	3.8	14.856
C18:0	1.9	18
C17:0 CYCLO	1.6	16.888
C20:0	1.6	20
C14:1 ω5c	1.3	13.899
C14:0 ISO 3OH	1	15.117
C17:0	1	17
C15:0	0.9	15
C19:0	0.5	19
C14:0	0.5	14
C17:1 ω9c ANTEISO	0.5	16.525

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #3193]	3609	-	+	+/-	-	-	-	-	+	+	-	-	+	-
[Ref.: #3193]	3608	-	+	+	+	+	+/-	-	-	+	-	-	+	-
[Ref.: #3193]	3607	-	-	-	-	-	-	-	+/-	+	-	-	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3193]	Sample type/isolated from	human lung tissue
[Ref.: #3193]	Geographic location (country and/or sea, region)	Philadelphia
[Ref.: #3193]	Country	USA
[Ref.: #3193]	Country ISO 3 Code	USA
[Ref.: #3193]	Continent	North America

[Ref.: #45204]	Sample type/isolated from	Human lung
[Ref.: #45204]	Geographic location (country and/or sea, region)	Philadelphia
[Ref.: #45204]	Country	USA
[Ref.: #45204]	Country ISO 3 Code	USA
[Ref.: #45204]	Continent	North America

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Lung
#Infection	#Patient	-

What are isolation sources categories?

* marker position based on {}

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3193]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Legionella pneumophila strain ATCC33152 16S-23S rRNA intergenic region with tRNA-Ala-like sequence	AF000655	448	ENA	446 tax ID	*
[Ref.: #20218]	Legionella pneumophila 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer and tRNA-Ala gene, complete sequence; and 23S ribosomal RNA gene, partial sequence	AY298787	3248	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 2 of 3	M19444	137	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 1 of 3	M19450	125	ENA	446 tax ID	*
[Ref.: #20218]	L.pneumophila (strain Philadelphia-1) 16S rRNA, partial, segment 3 of 3	M22266	141	ENA	446 tax ID	*
[Ref.: #20218]	Legionella pneumophila 16S ribosomal RNA	M59157	1544	ENA	446 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella pneumophila FDAARGOS_779	GCA_006364635	complete	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila Philadelphia-1	GCA_001941585	complete	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila D-4954	GCA_003003515	complete	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila L10-023	GCA_001610735	complete	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila NY24 (D-7706)	GCA_003003535	complete	GenBank	446 tax ID	*
[Ref.: #66792]	Legionella pneumophila NY23 (D-7705)	GCA_003003555	complete	GenBank	446 tax ID	*

[Ref.: #3193]

GC-content

39.0 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #3193]

Culture collection no.

DSM 7513, ATCC 33152, WDCM 00107, CCUG 9568, NCTC 11192

[Ref.: #20218]

Associated Passport(s) in StrainInfo

160885, 108916, 866305

References

#3193

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7513

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40120 ; Curators of the CIP;
#45204 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 9568

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These References are textmined

Change proposal

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