Name: Enterococcus faecium U0317
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 5303

Type strain:

Species: Enterococcus faecium

Strain Designation: U0317

Culture col. no.: DSM 25390

Strain history: <- W. van Schalk, University Medical Center Utrecht, Dept. of Med. Microbiol., The Netherlands; U0317

NCBI tax ID(s): 1352 (species)

SI-ID 405106

DSM 25390

Special Collections

DSMZ

Other strains from Enterococcus faecium

Section

Enterococcus faecium U0317 is a microaerophile bacterium that was isolated from clinical isolate .

microaerophile
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus faecium**
	Full Scientific Name (LPSN)	Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

Synonym

Streptococcus faecium

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %91.304

[Ref.: #18027]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18027]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #18027]

Culture medium growth

[Ref.: #18027]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18027]

Culture medium composition

expand / minimize

[Ref.: #18027]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #18027]

Culture medium growth

[Ref.: #18027]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18027]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #18027]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #18027]

Oxygen tolerance

microaerophile

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68370]	arginine ChEBI	+	hydrolysis	* from API 20STR, API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68370]	D-mannitol ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68370]	D-ribose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68370]	esculin ChEBI	+	hydrolysis	* from API 20STR
[Ref.: #68370]	glycogen ChEBI	-	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68370]	hippurate ChEBI	+	hydrolysis	* from API 20STR, API rID32STR
[Ref.: #68370]	inulin ChEBI	-	builds acid from	* from API 20STR
[Ref.: #68370]	L-arabinose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68370]	lactose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68370]	trehalose ChEBI	+	builds acid from	* from API 20STR, API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68370]		acetoin ChEBI	yes	* from API 20STR, API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR, API 20STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68381]	alkaline phosphatase	-	3.1.3.1	* from API rID32STR, API 20STR
[Ref.: #68381]	alpha-galactosidase	+	3.2.1.22	* from API rID32STR
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR, API 20STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR, API 20STR
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR, API 20STR
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR, API 20STR
[Ref.: #18027]	catalase	-	1.11.1.6
[Ref.: #18027]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68370]	leucine arylamidase	-	3.4.11.1	* from API 20STR
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

API® 20STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Acetoin production (Voges Proskauer test)VP	Hippuric acid hydrolysisHIP	Esculin hydrolysis/beta-glucosidaseESC	Pyrrolidonyl arylamidasePYRA	alpha-Galactosidasealpha GAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	Alkaline phosphatasePAL	Leucine arylamidaseLAP	Arginine dihydrolaseADH	Acid from D-riboseRIB	Acid from L-arabinoseARA	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	beta hemolysis presentbeta HEM
[Ref.: #18027]	852	+	+	+	-	-	-	+	-	-	+	+	+	+	-	+	+	-	+	+/-	-	not determinedn.d.
[Ref.: #18027]	851	+	+	+	+	+/-	-	+	-	-	+	+	+	+	+/-	+	+	-	-	+/-	-	not determinedn.d.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #18027]	10586	+	+	+	-	+	-	+	+	-	+	+	+	+	+	-	+	+	-	+	+	+	+	+	-	-	+	+	-	+	-	+	-
[Ref.: #18027]	10585	+	+	+	-	+	-	+	+	-	+	+	+	+	+	-	+	+	-	+	+	+	+	+	-	-	+	+	-	+	-	+	-
[Ref.: #18027]	10584	+	+	+	-	+	-	+	+	-	+	+	-	+	+	-	-	+	-	+	-	+/-	+	+	-	-	+	-	-	-	-	+/-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18027]	Sample type/isolated from	clinical isolate (urinary tract infection)
[Ref.: #18027]	Geographic location (country and/or sea, region)	Utrecht
[Ref.: #18027]	Country	Netherlands
[Ref.: #18027]	Country ISO 3 Code	NLD
[Ref.: #18027]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Infection	#Medical environment	#Clinic
#Host Body-Site	#Urogenital tract	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18027]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus faecium U0317	GCA_000172915	contig	GenBank ENA	547468 tax ID	*
[Ref.: #66792]	Enterococcus faecium U0317	547468.4	wgs	PATRIC	547468 tax ID	*
[Ref.: #66792]	Enterococcus faecium U0317	647000248	draft	JGI IMG/M	547468 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Enterococcus faecium U0317 PATRIC: 547468.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	91.304	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	89.681	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	77.984	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	96.643	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.5	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	87	no

Availability in culture collections External links

[Ref.: #18027]

Culture collection no.

DSM 25390

[Ref.: #74793]

SI-ID 405106

Literature:

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Detection and characterization of bacterial polysaccharides in drug-resistant enterococci.	Ali L, Blum HE, Sakiotanc T	Glycoconj J	10.1007/s10719-019-09881-3	2019	*
Pathogenicity	Identification and functional characterization of the putative polysaccharide biosynthesis protein (CapD) of Enterococcus faecium U0317.	Ali L, Spiess M, Wobser D, Rodriguez M, Blum HE, Sakiotanc T	Infect Genet Evol	10.1016/j.meegid.2015.11.020	2015	*
Phylogeny	Chemical structure of wall teichoic acid isolated from Enterococcus faecium strain U0317.	Bychowska A, Theilacker C, Czerwicka M, Marszewska K, Huebner J, Holst O, Stepnowski P, Kaczynski Z	Carbohydr Res	10.1016/j.carres.2011.09.026	2011	*

References

#18027

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 25390

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68370

Automatically annotated from API 20STR .

#68381

Automatically annotated from API rID32STR .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#74793

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405106.1 )

* These data were automatically processed and therefore are not curated

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