Enterococcus faecium | Type strain | DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

Strain identifier

BacDive ID: 5301

Type strain:

Species: Enterococcus faecium

Culture col. no.: DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

[Ref.: #8846]	Domain	Bacteria
[Ref.: #8846]	Phylum	Firmicutes
[Ref.: #8846]	Class	Bacilli
[Ref.: #8846]	Order	Lactobacillales
[Ref.: #8846]	Family	Enterococcaceae
[Ref.: #8846]	Genus	Enterococcus
[Ref.: #8846]	Species	Enterococcus faecium
[Ref.: #8846]	Full Scientific Name	Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984
[Ref.: #8846]	Type strain

		Prokaryotic Nomenclature Up-to-date (PNU)	Taxonomical status	Literature reference
	Last PNU update	25-03-2019 (DD-MM-YYYY)
[Ref.: #20215]	Domain	Bacteria
[Ref.: #20215]	Phylum	Firmicutes
[Ref.: #20215]	Class	Bacilli		Int. J. Syst. Evol. Microbiol. 60:469
[Ref.: #20215]	Family	Enterococcaceae		Int. J. Syst. Evol. Microbiol. 60:469
[Ref.: #20215]	Genus	Enterococcus	gen. nov. (VP)	Int. J. Syst. Bacteriol. 34:31*
[Ref.: #20215]	Species	Enterococcus faecium	comb. nov. (VP)	Int. J. Syst. Bacteriol. 34:31*
[Ref.: #20215]	Full Scientific Name	Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

[Ref.: #20215]

Synonym

Streptococcus faecium

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #8846]	Type of hemolysis	alpha
[Ref.: #8846]	Incubation period	1-2 days

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #8846]		cytochrome-c oxidase	-	1.9.3.1

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #8846]	2613	+	+	-	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	2290	+	+	+/-	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	-	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	2289	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	2288	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	not determinedn.d.	-	-	-	-	+	-	-	+	-	-	+	-	+	-

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Test 6

Reference

[Ref.: #8846]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22
12
28
24-26
22
14
8
14
resistant0
24
30
26
18
18
24
18
28
12-14
16
resistant0
resistant0
16-18
14
resistant0
24-26
16-18
32-34
16
32
16-18
14
24-26
resistant0
28
18-20
20

[Ref.: #8846]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
26
16
30
28
24-26
18
10
20-22
resistant0
26-28
34
28
16-18
18
24
20
30
16
18
resistant0
resistant0
16-18
14-16
6
14
18
34
20
34
16
18
32
resistant0
30
20
22

[Ref.: #8846]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
26
14-16
30
28
24
18
12-14
20
resistant0
26
34
26
16
14-16
22
16
30
14-16
18
resistant0
resistant0
14
16
resistant0
12
14
32
20-22
32
18
16
30
resistant0
26
18
22-24

[Ref.: #8846]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
16
resistant0
16
16
10
6
resistant0
resistant0
resistant0
18-20
18
18
12
12
14
10
18-20
resistant0
10
resistant0
resistant0
resistant0
10
resistant0
10
10
20
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #8846]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22-24
12
28
26-28
24
16
12
14
resistant0
28
34-36
28
18
18
26
20
30
14-16
16-18
resistant0
resistant0
16-18
16
resistant0
24-26
18
34
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #8846]
Mueller-Hinton Agar
37

aerob
yes
Inhibition zone diameter in mm
24
14
26-28
24-26
20-22
16
8-10
16-18
resistant0
24
30-32
24-26
16
16
20
16-18
26-28
14
16
resistant0
resistant0
8-10
12-14
resistant0
12
16
30
18-20
30
16
16
28
resistant0
24-26
18
20-22

Only first 15 entries are displayed. Click here to see all.

[Ref.: #8846]	Murein short key	A11.31
[Ref.: #8846]	Murein types	A4alpha L-Lys-D-Asp

[Ref.: #8846]

Oxygen tolerance

microaerophile

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #8846]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), 37°C, microaerophilic

[Ref.: #8846]

Culture medium growth

[Ref.: #8846]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #8846]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693), 37°C, microaerophilic

[Ref.: #8846]

Culture medium growth

[Ref.: #8846]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #41268]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41268]

Culture medium growth

[Ref.: #41268]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8846]			growth	37 ̊C
[Ref.: #41268]			growth	37 ̊C

[Ref.: #8846]	Temperature range	mesophilic
[Ref.: #41268]	Temperature range	mesophilic

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #8846]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium insertion sequence IS1485, complete sequence	AF029727	1366	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium clone pEMH19 insertion sequence IS1485 left junction	AF029770	195	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium clone pEMH19 insertion sequence IS1485 right junction	AF029771	758	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium putative elongation factor Tu (tuf) gene, partial cds	AF124222	751	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium 60 kDa chaperonin (cpn60) gene, partial cds	AF245681	552	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium 23S ribosomal RNA gene, partial sequence	AF273486	301	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium elongation factor Tu (tufB) gene, partial cds	AF274724	751	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium ATCC 19434 GroES and GroEL genes, partial cds	AF417582	1904	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain DSM 20477 RpoB gene, partial cds	AF515643	323	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Enterococcus faecium 16S rRNA gene, strain DSM20477	AJ276355	1533	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Enterococcus faecium partial sodA gene for superoxide dismutase, strain CIP 103014 T (ATCC 19434)	AJ387913	438	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 292 from Patent WO0123604	AX109559	831	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 608 from Patent WO0123604	AX109875	751	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 621 from Patent WO0123604	AX109888	751	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 1870 from Patent WO0123604	AX111137	388	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 8 from Patent WO0188186	AX354197	438	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 16S-23S ribosomal RNA intergenic spacer, partial sequence	AY351322	344	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium DnaK (dnaK) gene, partial cds; dnaK-dnaJ intergenic spacer, complete sequence; and DnaJ (dnaJ) gene, partial cds	DQ176870	600	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 16S ribosomal RNA gene, partial sequence	DQ411813	1482	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 ATP synthase beta-subunit (atpD) gene, partial cds	GU457278	718	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain DSM 20477 16S ribosomal RNA gene, partial sequence	HQ012007	936	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 DNA-directed RNA polymerase beta subunit (rpoB) gene, partial cds	HQ611243	1177	1352	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 16S ribosomal RNA gene, partial sequence	KC510233	815	1352	*
[Ref.: #20218]	INSDC Sequence	Enterococcus faecium genomic DNA fragment	L78127	1225	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	E.faecium 16S-23S rRNA spacer DNA, strain ATCC 19434	X87180	344	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	E.faecium 23S-5S rRNA spacer DNA, strain ATCC 19434	X87181	94	1352	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	E.faecium 16S rRNA gene, partial (strain ATTC 19434 (CCUG 542))	Y12906	366	1352	*
[Ref.: #8846]	INSDC	Enterococcus faecium gene for 16S ribosomal RNA, partial sequence, strain: JCM 8727	LC096216	1499	1352

Availability in culture collectionsStrain availability

[Ref.: #8846]

Culture collection no.

DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

[Ref.: #8846]

Strain history

<- NCDO <- NCTC <- A. Grumbach

[Ref.: #20218]

Associated Passport(s) in StrainInfo

9243, 9238, 119643, 9250, 866224

References

#8846

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20477

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41268 ; Curators of the CIP;
* These References are textmined

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