Name: Enterococcus faecium DSM 20477
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5301

Type strain:

Species: Enterococcus faecium

Culture col. no.: DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

Strain history: <- NCDO <- NCTC <- A. Grumbach

NCBI tax ID(s): 1352 (species)

Other strains from Enterococcus faecium

Section

Enterococcus faecium DSM 20477 is a microaerophile, mesophilic bacterium that has multiple antibiotic resistances.

antibiotic resistance
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	23-11-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Firmicutes
	Class	Bacilli
	Order	Lactobacillales
	Family	Enterococcaceae
	Genus	Enterococcus
	Species	Enterococcus faecium
	Full Scientific Name (PNU)	Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

Synonym

Streptococcus faecium

Information on morphological and physiological properties Morphology

[Ref.: #8846]	Type of hemolysis	alpha
[Ref.: #8846]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8846]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693), microaerophilic

[Ref.: #8846]

Culture medium growth

[Ref.: #8846]

Culture medium link

Medium recipe at BacMedia

[Ref.: #8846]

Culture medium composition

expand / minimize

[Ref.: #8846]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), microaerophilic

[Ref.: #8846]

Culture medium growth

[Ref.: #8846]

Culture medium link

Medium recipe at BacMedia

[Ref.: #8846]

Culture medium composition

expand / minimize

[Ref.: #41268]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41268]

Culture medium growth

[Ref.: #41268]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8846]			growth	37 °C
[Ref.: #41268]			growth	37 °C

[Ref.: #8846]	Temperature range	mesophilic
[Ref.: #41268]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8846]

Oxygen tolerance

microaerophile

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Test 6

Test 7

Reference

[Ref.: #8846]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22
12
28
24-26
22
14
8
14
resistant0
24
30
26
18
18
24
18
28
12-14
16
resistant0
resistant0
16-18
14
resistant0
24-26
16-18
32-34
16
32
16-18
14
24-26
resistant0
28
18-20
20

[Ref.: #8846]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
26
16
30
28
24-26
18
10
20-22
resistant0
26-28
34
28
16-18
18
24
20
30
16
18
resistant0
resistant0
16-18
14-16
6
14
18
34
20
34
16
18
32
resistant0
30
20
22

[Ref.: #8846]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
26
14-16
30
28
24
18
12-14
20
resistant0
26
34
26
16
14-16
22
16
30
14-16
18
resistant0
resistant0
14
16
resistant0
12
14
32
20-22
32
18
16
30
resistant0
26
18
22-24

[Ref.: #8846]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
16
resistant0
16
16
10
6
resistant0
resistant0
resistant0
18-20
18
18
12
12
14
10
18-20
resistant0
10
resistant0
resistant0
resistant0
10
resistant0
10
10
20
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #8846]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22-24
12
28
26-28
24
16
12
14
resistant0
28
34-36
28
18
18
26
20
30
14-16
16-18
resistant0
resistant0
16-18
16
resistant0
24-26
18
34
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #8846]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
22
12-14
26-28
22-24
20
16
8
20
resistant0
24
30
26
16
16
20
18
26-28
14
14-16
resistant0
resistant0
10
14
resistant0
12-14
16
30
18
30
14
14
28
resistant0
26
16-18
20

[Ref.: #8846]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
22
12-14
26-28
22-24
20
16
8
20
resistant0
24
30
26
16
16
20
18
26-28
14
14-16
resistant0
resistant0
10
14
resistant0
12-14
16
30
18
30
14
14
28
resistant0
26
16-18
20

Only first 15 entries are displayed. Click here to see all.

[Ref.: #8846]	Murein short key	A11.31
[Ref.: #8846]	Murein types	A4alpha L-Lys-D-Asp

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #8846]		cytochrome-c oxidase	-	1.9.3.1

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #8846]	7959	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	+	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	7905	+	+	-	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	7576	+	+	+/-	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	-	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	7575	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	7574	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	not determinedn.d.	-	-	-	-	+	-	-	+	-	-	+	-	+	-

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #44225]	1930	-	+	+	+	+	-	-	-	-	+	-	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8846]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Enterococcus faecium 16S rRNA gene, strain DSM20477	AJ276355	1533	ENA	1352 tax ID	*
[Ref.: #20218]	Enterococcus faecium strain ATCC 19434 16S-23S ribosomal RNA intergenic spacer, partial sequence	AY351322	344	ENA	1352 tax ID	*
[Ref.: #20218]	Enterococcus faecium strain ATCC 19434 16S ribosomal RNA gene, partial sequence	DQ411813	1482	ENA	1352 tax ID	*
[Ref.: #20218]	Enterococcus faecium strain DSM 20477 16S ribosomal RNA gene, partial sequence	HQ012007	936	ENA	1352 tax ID	*
[Ref.: #20218]	Enterococcus faecium strain ATCC 19434 16S ribosomal RNA gene, partial sequence	KC510233	815	ENA	1352 tax ID	*
[Ref.: #20218]	E.faecium 16S-23S rRNA spacer DNA, strain ATCC 19434	X87180	344	ENA	1352 tax ID	*
[Ref.: #20218]	E.faecium 16S rRNA gene, partial (strain ATTC 19434 (CCUG 542))	Y12906	366	ENA	1352 tax ID	*
[Ref.: #8846]	Enterococcus faecium gene for 16S ribosomal RNA, partial sequence, strain: JCM 8727	LC096216	1499	ENA	1352 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Enterococcus faecium NCTC7171	GCA_900447735	contig	GenBank	1352 tax ID	*
[Ref.: #66792]	Enterococcus faecium strain NCTC7171	1352.4067	wgs	PATRIC	1352 tax ID	*

Availability in culture collections External links

[Ref.: #8846]

Culture collection no.

DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

[Ref.: #20218]

Associated Passport(s) in StrainInfo

9243, 9238, 119643, 9250, 866224

References

#8846

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20477

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41268 ; Curators of the CIP;
#44225 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 542

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These References are textmined

Change proposal

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