Enterococcus faecium | Type strain | DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

Strain identifier

BacDive ID: 5301

Type strain:

Species: Enterococcus faecium

Culture col. no.: DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

Section

Name and taxonomic classification
Morphology and physiology
Culture and growth conditions
Application and interaction
Molecular biology
Strain availability

External links

Information on the name and the taxonomic classification.Name and taxonomic classification

	Last LPSN update	31-03-2020 (DD-MM-YYYY)
	Domain	Bacteria
	Phylum	Firmicutes
	Class	Bacilli
	Order	Lactobacillales
	Family	Enterococcaceae
	Genus	Enterococcus
	Species	Enterococcus faecium
	Full Scientific Name (PNU)	Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

Synonym

Streptococcus faecium

Information on morphological and physiological propertiesMorphology and physiology

[Ref.: #8846]	Type of hemolysis	alpha
[Ref.: #8846]	Incubation period	1-2 days

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #8846]		cytochrome-c oxidase	-	1.9.3.1

API® NH:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Penicillinase productionPEN	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from maltoseMAL	Acid from sucroseSAC	Ornithine decarboxylaseODC	Urease/urea hydrolysisURE	LipaseLIP	Alkaline phosphatasePAL	beta-Galactosidasebeta GAL	Proline arylamidaseProA	gamma-Glutamyl transferaseGGT	Indole productionIND
[Ref.: #44225]	1930	-	+	+	+	+	-	-	-	-	+	-	-	-

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #8846]	6574	+	+	-	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	6254	+	+	+/-	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	-	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	6253	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	6252	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	not determinedn.d.	-	-	-	-	+	-	-	+	-	-	+	-	+	-
[Ref.: #8846]	6628	+	+	+	-	-	-	+	+	-	+	+	-	+	+	-	+	+	-	+	+	+	+	+	-	-	+	-	-	+	-	+	-

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Test 6

Reference

[Ref.: #8846]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22
12
28
24-26
22
14
8
14
resistant0
24
30
26
18
18
24
18
28
12-14
16
resistant0
resistant0
16-18
14
resistant0
24-26
16-18
32-34
16
32
16-18
14
24-26
resistant0
28
18-20
20

[Ref.: #8846]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
26
16
30
28
24-26
18
10
20-22
resistant0
26-28
34
28
16-18
18
24
20
30
16
18
resistant0
resistant0
16-18
14-16
6
14
18
34
20
34
16
18
32
resistant0
30
20
22

[Ref.: #8846]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
26
14-16
30
28
24
18
12-14
20
resistant0
26
34
26
16
14-16
22
16
30
14-16
18
resistant0
resistant0
14
16
resistant0
12
14
32
20-22
32
18
16
30
resistant0
26
18
22-24

[Ref.: #8846]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
16
resistant0
16
16
10
6
resistant0
resistant0
resistant0
18-20
18
18
12
12
14
10
18-20
resistant0
10
resistant0
resistant0
resistant0
10
resistant0
10
10
20
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #8846]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
22-24
12
28
26-28
24
16
12
14
resistant0
28
34-36
28
18
18
26
20
30
14-16
16-18
resistant0
resistant0
16-18
16
resistant0
24-26
18
34
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.
n.d.

[Ref.: #8846]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
22
12-14
26-28
22-24
20
16
8
20
resistant0
24
30
26
16
16
20
18
26-28
14
14-16
resistant0
resistant0
10
14
resistant0
12-14
16
30
18
30
14
14
28
resistant0
26
16-18
20

Only first 15 entries are displayed. Click here to see all.

[Ref.: #8846]	Murein short key	A11.31
[Ref.: #8846]	Murein types	A4alpha L-Lys-D-Asp

[Ref.: #8846]

Oxygen tolerance

microaerophile

Information on culture and growth conditionsCulture and growth conditions

[Ref.: #8846]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693), 37°C, microaerophilic

[Ref.: #8846]

Culture medium growth

[Ref.: #8846]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #8846]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92), 37°C, microaerophilic

[Ref.: #8846]

Culture medium growth

[Ref.: #8846]

Culture medium link

medium recipe provided by DSMZ

[Ref.: #41268]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41268]

Culture medium growth

[Ref.: #41268]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #8846]			growth	37 ̊C
[Ref.: #41268]			growth	37 ̊C

[Ref.: #8846]	Temperature range	mesophilic
[Ref.: #41268]	Temperature range	mesophilic

Information on possible application of the strain and its possible interaction with e.g. potential hostsApplication and interaction

[Ref.: #8846]

Biosafety level

Risk group (German classification)

Information on genomic background e.g. entries in nucleic sequence databassMolecular biology

Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence database	Sequence accession description	Sequence accession number	Sequence type	Sequence length (bp)	Associated NCBI tax ID
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium insertion sequence IS1485, complete sequence	AF029727 ENA		1366	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium clone pEMH19 insertion sequence IS1485 left junction	AF029770 ENA		195	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium clone pEMH19 insertion sequence IS1485 right junction	AF029771 ENA		758	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium putative elongation factor Tu (tuf) gene, partial cds	AF124222 ENA		751	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium 60 kDa chaperonin (cpn60) gene, partial cds	AF245681 ENA		552	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium 23S ribosomal RNA gene, partial sequence	AF273486 ENA		301	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium elongation factor Tu (tufB) gene, partial cds	AF274724 ENA		751	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium ATCC 19434 GroES and GroEL genes, partial cds	AF417582 ENA		1904	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain DSM 20477 RpoB gene, partial cds	AF515643 ENA		323	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Enterococcus faecium 16S rRNA gene, strain DSM20477	AJ276355 ENA	16S	1533	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Enterococcus faecium partial sodA gene for superoxide dismutase, strain CIP 103014 T (ATCC 19434)	AJ387913 ENA		438	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 292 from Patent WO0123604	AX109559 ENA		831	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 608 from Patent WO0123604	AX109875 ENA		751	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 621 from Patent WO0123604	AX109888 ENA		751	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 1870 from Patent WO0123604	AX111137 ENA		388	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	Sequence 8 from Patent WO0188186	AX354197 ENA		438	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 16S-23S ribosomal RNA intergenic spacer, partial sequence	AY351322 ENA	16S	344	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium DnaK (dnaK) gene, partial cds; dnaK-dnaJ intergenic spacer, complete sequence; and DnaJ (dnaJ) gene, partial cds	DQ176870 ENA		600	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 16S ribosomal RNA gene, partial sequence	DQ411813 ENA	16S	1482	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 ATP synthase beta-subunit (atpD) gene, partial cds	GU457278 ENA		718	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain DSM 20477 16S ribosomal RNA gene, partial sequence	HQ012007 ENA	16S	936	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 DNA-directed RNA polymerase beta subunit (rpoB) gene, partial cds	HQ611243 ENA		1177	1352 tax ID	*
[Ref.: #20218]	Marker Gene (GenBank Direct submission)	Enterococcus faecium strain ATCC 19434 16S ribosomal RNA gene, partial sequence	KC510233 ENA	16S	815	1352 tax ID	*
[Ref.: #20218]	INSDC Sequence	Enterococcus faecium genomic DNA fragment	L78127 ENA		1225	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	E.faecium 16S-23S rRNA spacer DNA, strain ATCC 19434	X87180 ENA	16S	344	1352 tax ID	*
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	E.faecium 23S-5S rRNA spacer DNA, strain ATCC 19434	X87181 ENA		94	1352 tax ID	*
[Ref.: #8846]	16S rRNA gene, Marker Gene (DDBJ Direct submission)	Enterococcus faecium gene for 16S ribosomal RNA, partial sequence, strain: JCM 8727	LC096216 ENA	16S	1499	1352 tax ID
[Ref.: #20218]	Marker Gene (EMBL Direct submission)	E.faecium 16S rRNA gene, partial (strain ATTC 19434 (CCUG 542))	Y12906 ENA	16S	366	1352 tax ID	*

Availability in culture collectionsStrain availability

[Ref.: #8846]

Culture collection no.

DSM 20477, ATCC 19434, NCDO 942, NCTC 7171, WDCM 00010, CCUG 542, LMG 8149, LMG 11423, CCM 7167

[Ref.: #8846]

Strain history

<- NCDO <- NCTC <- A. Grumbach

[Ref.: #20218]

Associated Passport(s) in StrainInfo

9243, 9238, 119643, 9250, 866224

References

#8846

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20477

#20215

Leibniz Institute DSMZ D.Gleim, M.Kracht, N.Weiss et. al.: Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#41268 ; Curators of the CIP;
#44225 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 542
* These References are textmined

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