Name: Enterococcus faecium DSM 2146
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5295

Type strain:

Species: Enterococcus faecium

Culture col. no.: DSM 2146, ATCC 6057, WDCM 00177, NBRC 113009, CIP 106742, CCM 2308, CECT 8108

Strain history: CIP <- 2000, ATCC <- J.M. Sherman, Streptocccus faecalis

NCBI tax ID(s): 1352 (species)

SI-ID 8777

LMG 15709, ATCC 6057, CCM 2308, DSM 2146, NCIMB 8842, CIP 106742, CCUG 34228, KCTC 3077, KCTC 3513, CCUG 46351, BCRC 17012, CCRC 17012, CGMCC 1.2025, CECT 8108

Other strains from Enterococcus faecium

Section

Enterococcus faecium DSM 2146 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from cheese.

coccus-shaped
Gram-positive
mesophilic
microaerophile
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus faecium**
	Full Scientific Name (LPSN)	Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

Synonym

Streptococcus faecium

Information on morphological and physiological properties Morphology

[Ref.: #119264]	Gram stain	positive

[Ref.: #119264]	Cell shape	coccus-shaped

[Ref.: #119264]	Motility	no

[Ref.: #977]

Incubation period

1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #977]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #977]

Culture medium growth

[Ref.: #977]

Culture medium link

Medium recipe at MediaDive

[Ref.: #977]

Culture medium composition

expand / minimize

[Ref.: #977]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #977]

Culture medium growth

[Ref.: #977]

Culture medium link

Medium recipe at MediaDive

[Ref.: #977]

Culture medium composition

expand / minimize

[Ref.: #41995]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #41995]

Culture medium growth

[Ref.: #41995]

Culture medium composition

expand / minimize

[Ref.: #119264]

Culture medium

CIP Medium 72

[Ref.: #119264]

Culture medium growth

[Ref.: #119264]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119264]

Culture medium

CIP Medium 111

[Ref.: #119264]

Culture medium growth

[Ref.: #119264]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #119264]

Culture medium

CIP Medium 29

[Ref.: #119264]

Culture medium growth

[Ref.: #119264]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #977]		growth	37 °C
[Ref.: #41995]		growth	37 °C
[Ref.: #119264]		growth	25-45 °C
[Ref.: #119264]	no	growth	10 °C

[Ref.: #977]	Temperature range	mesophilic
[Ref.: #41995]	Temperature range	mesophilic
[Ref.: #119264]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #977]	Oxygen tolerance	microaerophile
[Ref.: #119264]	Oxygen tolerance	facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Test 4

Test 5

Test 6

Test 7

Reference

[Ref.: #977]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
30
22
30-32
30-32
28
24-26
24
26-28
resistant0
30
30-32
26
16
14
22
18
24
16
18
resistant0
resistant0
12
12
resistant0
10
14-16
30-32
24-26
30
20
22
30
resistant0
24-26
20
28

[Ref.: #977]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
32
24
34
34
30
28
26-28
28
resistant0
32
32-34
26-28
14
14
22
18
24-26
16
18-20
resistant0
resistant0
10
16
resistant0
10
14-16
30
26
30
22
22
28
resistant0
24
18
28

[Ref.: #977]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
30
24
32
30-32
30
26
26
28
resistant0
32
32
26-28
14
14
22
18
26
16
18
resistant0
resistant0
12-14
16
resistant0
12
14
30
26
32
20
22
22-24
resistant0
24
20
26

[Ref.: #977]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
30-32
22-24
32
32
30
26-28
18
28
resistant0
32
30
28
16-18
16-18
22
18
26
16
18
resistant0
resistant0
16-18
16
resistant0
12
16-18
32
26-28
32
20
22
24-26
resistant0
24-26
18-20
26-28

[Ref.: #977]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
28-30
20
32
30-32
28
26
20-22
26-28
resistant0
30-32
32
26
16-18
16
24
18
26
18
20
resistant0
resistant0
18
14
resistant0
24
16
28-30
26-28
32
24
22
24
resistant0
24-26
20
26

[Ref.: #977]
Mueller-Hinton Agar



yes
Inhibition zone diameter in mm
30
22
32
32-34
30
28
22-24
30
resistant0
32-34
34-36
28-30
16
14
24
18
26
18
20
resistant0
resistant0
18
16-18
resistant0
24
14
32
28
30
22
22
28
resistant0
26
20
24-26

[Ref.: #977]
Mueller Hinton
37

microaerophil
yes
Inhibition zone diameter in mm
30
22
30
30
28
24
22
24-26
resistant0
30
26-28
26
16
14
20-22
18
24
16
18
resistant0
resistant0
18
12
resistant0
12
14-16
30
24-26
28
16-18
22
28-30
resistant0
24
18
24-26

Only first 15 entries are displayed. Click here to see all.

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #119264]		NaCl		growth	6.5	%

[Ref.: #977]	Murein short key	A11.31
[Ref.: #977]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #119264]	esculin ChEBI	+	hydrolysis
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #119264]	hippurate ChEBI	-	hydrolysis
[Ref.: #68381]	L-arabinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	melibiose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #119264]	nitrate ChEBI	-	reduction
[Ref.: #119264]	nitrite ChEBI	-	reduction
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #119264]		acetoin ChEBI	-
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #119264]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API rID32STR
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym, API rID32STR
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #119264]	beta-galactosidase	-	3.2.1.23
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API rID32STR
[Ref.: #977]	catalase	-	1.11.1.6
[Ref.: #119264]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #977]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #119264]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119264]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119264]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119264]	oxidase	-
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #977]	10862	+	+	-	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #977]	10174	+	+	-	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	-	-	-	-	-	+	-	-	+/-	-	-	-
[Ref.: #977]	10175	+	+	+	-	-	-	+	+	-	+	+	-	-	+	-	-	+	-	+	+	+	+	-	-	-	+	-	-	-	-	+	-
[Ref.: #977]	10176	+	+	+/-	-	-	-	+	+	-	+	+	-	-	+	-	+/-	+	-	+	+	+	+	-	-	-	+	-	-	-	-	+	-
[Ref.: #977]	10177	+	+	+/-	-	-	-	+	+	-	+	+	-	-	+	-	-	+	-	+	+	+	+	-	-	-	+	-	-	-	-	+	-
[Ref.: #977]	10178	+	+	-	-	-	-	+	+	-	+	+	-	-	+	-	-	+	-	+	+	-	+	+	-	-	+	-	-	+/-	-	-	-
[Ref.: #977]	10179	+	+	-	-	-	-	+	+	-	+	+	-	-	+	-	-	+	-	+	+	-	-	-	-	-	+	-	-	-	-	+/-	-
[Ref.: #977]	10714	+	+	-	-	-	-	+/-	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	+	-	-	+	-	-	+/-	-	+	-
[Ref.: #977]	10766	+	+	+	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	+	-	-	+	-	-	+	-	+	-
[Ref.: #977]	10899	+	+	-	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+/-	+/-	-	-	-	+	-	-	+	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119264]	9770	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #977]	Sample type/isolated from	cheese

[Ref.: #119264]	Sample type/isolated from	Food, Cheese
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Dairy product

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #977]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119264]	Biosafety level	2	Risk group (French classification)

Availability in culture collections External links

[Ref.: #977]

Culture collection no.

DSM 2146, ATCC 6057, WDCM 00177, NBRC 113009, CIP 106742, CCM 2308, CECT 8108

[Ref.: #74785]

SI-ID 8777

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Enterococcus hirae, Enterococcus faecium and Enterococcus faecalis show different sensitivities to typical biocidal agents used for disinfection.	Suchomel M, Lenhardt A, Kampf G, Grisold A	J Hosp Infect	10.1016/j.jhin.2019.08.014	2019	*
Pathogenicity	Isopropanol at 60% and at 70% are effective against 'isopropanol-tolerant' Enterococcus faecium.	Gebel J, Gemein S, Kampf G, Pidot SJ, Buetti N, Exner M	J Hosp Infect	10.1016/j.jhin.2019.01.024	2019	*
Pathogenicity	Resistance of enterococci to heat and chemical agents.	Renner P, Peters J	Zentralbl Hyg Umweltmed		1999	*
Pathogenicity	[Inactivation of chlorhexidine for in vitro testing of disinfectants].	Kampf G, Hofer M, Ruden H	Zentralbl Hyg Umweltmed		1998	*
Pathogenicity	Disinfection with gaseous formaldehyde. Third Part: Bactericidal and sporicidal effectiveness of gaseous formaldehyde and level of residues in dependence on concentration, temperature and relative humidity.	Casella ML, Schmidt-Lorenz W	Zentralbl Hyg Umweltmed		1989	*
Pathogenicity	Disinfection with gaseous formaldehyde. First Part: Bactericidal and sporicidal effectiveness of formaldehyde with and without formation of a condensing layer.	Casella ML, Schmidt-Lorenz W	Zentralbl Hyg Umweltmed		1989	*
	Requirements and infection prophylaxis for internally cooled implant drills.	Proff P, Bayerlein T, Kramer A, Allegrini S Jr, Dietze S, Fanghanel J, Gedrange T	Folia Morphol (Warsz)		2006	*
	Oxazoline scaffold in synthesis of benzosiloxaboroles and related ring-expanded heterocycles: diverse reactivity, structural peculiarities and antimicrobial activity.	Krajewska J, Nowicki K, Durka K, Marek-Urban PH, Winska P, Stepniewski T, Wozniak K, Laudy AE, Lulinski S	RSC Adv	10.1039/d2ra03910a	2022	*

References

#977	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2146

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41995 ; Curators of the CIP;

#68381

Automatically annotated from API rID32STR .

#68382

Automatically annotated from API zym .

#74785

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8777.1 )

#119264 Collection of Institut Pasteur ; Curators of the CIP; CIP 106742
* These data were automatically processed and therefore are not curated

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