Name: Enterococcus faecium 70/90
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 5299

Type strain:

Species: Enterococcus faecium

Strain Designation: 70/90, vanA

Culture col. no.: DSM 13590, CIP 110055, WDCM 00154, CECT 8224, NCTC 13633

Strain history: CIP <- 2009, DSMZ <- G. Rueter: strain 70/90 <- W. Witte and I. Klare, RKI <- J. Wagner, Freie Univ. Berlin, Germany: strain vanA

NCBI tax ID(s): 1352 (species)

SI-ID 143343

DSM 13590, CIP 110055, CECT 8224

Special Collections

Other strains from Enterococcus faecium

Section

When using BacDive for research please cite our paper

Enterococcus faecium 70/90 is a microaerophile, mesophilic, Gram-positive bacterium that has multiple antibiotic resistances and was isolated from human faeces, rectal swab.

antibiotic resistance
coccus-shaped
Gram-positive
mesophilic
microaerophile
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Enterococcaceae*
	Genus	*Enterococcus*
	Species	**Enterococcus faecium**
	Full Scientific Name (LPSN)	Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

Synonym

Streptococcus faecium

Information on morphological and physiological properties Morphology

[Ref.: #119226]	Gram stain	positive

[Ref.: #119226]	Cell shape	coccus-shaped

[Ref.: #119226]	Motility	no

[Ref.: #5098]	Type of hemolysis	alpha
[Ref.: #5098]	Hemolysis ability	1
[Ref.: #5098]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5098]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #5098]

Culture medium growth

[Ref.: #5098]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5098]

Culture medium composition

expand / minimize

[Ref.: #5098]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #5098]

Culture medium growth

[Ref.: #5098]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5098]

Culture medium composition

expand / minimize

[Ref.: #36873]

Culture medium

MEDIUM 29- Brain heart agar

[Ref.: #36873]

Culture medium growth

[Ref.: #36873]

Culture medium composition

expand / minimize

[Ref.: #119226]

Culture medium

CIP Medium 29

[Ref.: #119226]

Culture medium growth

[Ref.: #119226]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #5098]			growth	37 °C
[Ref.: #36873]			growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5098]	Oxygen tolerance	microaerophile
[Ref.: #119226]	Oxygen tolerance	facultative anaerobe

Note that the displayed test results represent raw data and therefore may deviate!

Antibiotic susceptibility testing

Test 1

Test 2

Test 3

Reference

[Ref.: #5098]
Mueller-Hinton Agar
37
1
microaerophil
yes
Inhibition zone diameter in mm
resistant0
resistant0
12
resistant0
10
resistant0
resistant0
resistant0
resistant0
6
10
24
12
12-14
resistant0
resistant0
resistant0
18
20
resistant0
resistant0
12
resistant0
resistant0
10
12-14
18
resistant0
resistant0
20
26
30
resistant0
18
6-8
6-8

[Ref.: #5098]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
resistant0
resistant0
12
resistant0
10
resistant0
resistant0
resistant0
resistant0
10
resistant0
22
12
14
resistant0
resistant0
resistant0
18
20
resistant0
resistant0
10
resistant0
resistant0
12
14
16
resistant0
resistant0
20
24
28
resistant0
18
resistant0
6

[Ref.: #5098]
Mueller-Hinton Agar
37

microaerophil
yes
Inhibition zone diameter in mm
resistant0
resistant0
12
resistant0
10
resistant0
resistant0
resistant0
resistant0
10
resistant0
22
12
14
resistant0
resistant0
resistant0
18
20
resistant0
resistant0
10
resistant0
resistant0
12
14
16
resistant0
resistant0
20
24
28
resistant0
18
resistant0
6

Only first 15 entries are displayed. Click here to see all.

[Ref.: #5098]

Observation

Vancomycin resistant

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68381]	alpha-cyclodextrin ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	arginine ChEBI	+	hydrolysis	* from API rID32STR
[Ref.: #68381]	D-arabitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	D-mannitol ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-ribose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	D-tagatose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	glycogen ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	L-arabinose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	lactose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	maltose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	melezitose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	methyl beta-D-glucopyranoside ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #119226]	nitrate ChEBI	-	reduction
[Ref.: #119226]	nitrite ChEBI	-	reduction
[Ref.: #68381]	pullulan ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	raffinose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sorbitol ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	sucrose ChEBI	-	builds acid from	* from API rID32STR
[Ref.: #68381]	trehalose ChEBI	+	builds acid from	* from API rID32STR
[Ref.: #68381]	urea ChEBI	-	hydrolysis	* from API rID32STR
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119226]		indole ChEBI	no
[Ref.: #68381]		acetoin ChEBI	yes	* from API rID32STR

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #68381]		acetoin ChEBI	+	* from API rID32STR

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68381]	Alanyl-Phenylalanyl-Proline arylamidase	-		* from API rID32STR
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API rID32STR
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68381]	arginine dihydrolase	+	3.5.3.6	* from API rID32STR
[Ref.: #68381]	beta-galactosidase	+	3.2.1.23	* from API rID32STR
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68381]	beta-glucosidase	+	3.2.1.21	* from API rID32STR
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #68381]	beta-glucuronidase	-	3.2.1.31	* from API rID32STR
[Ref.: #5098]	catalase	-	1.11.1.6
[Ref.: #119226]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #5098]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68381]	glycyl tryptophan arylamidase	+		* from API rID32STR
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68381]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API rID32STR
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119226]	oxidase	-
[Ref.: #68381]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API rID32STR
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119226]	urease	-	3.5.1.5
[Ref.: #68381]	urease	-	3.5.1.5	* from API rID32STR
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32STR:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Arginine dihydrolaseADH (Arg)	beta-Glucosidasebeta GLU	beta-Galactosidasebeta GAR	beta-Glucuronidasebeta GUR	alpha-Galactosidasealpha GAL	Alkaline PhosphatasePAL	Acid from D-riboseRIB	Acid from D-mannitolMAN	Acid from sorbitolSOR	Acid from lactoseLAC	Acid from trehaloseTRE	Acid from raffinoseRAF	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta-Galactosidasebeta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	Hydrolysis of hippuric acidHIP	Acid from glycogenGLYG	Acid from pullulanPUL	Acid from maltoseMAL	Acid from melibioseMEL	Acid from melezitoseMLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	Acid from D-tagatoseTAG	beta-Mannosidasebeta MAN	Urease/urea hydrolysisURE
[Ref.: #5098]	11628	+	+	-	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	+	-	-	+	-	-	+	-	-	-
[Ref.: #5098]	11539	+	+	+	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	+	-	-	+	-	-	+	-	+	-
[Ref.: #5098]	11035	+	+	-	-	+/-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	-	-	-	+	+	-	+	-	-	-
[Ref.: #5098]	11034	+	+	+	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	-	-	-	+	-	-	+	-	+	-
[Ref.: #5098]	11033	+	+	+	-	-	-	+	+	-	+	+	-	-	+	-	+	+	-	+	+	+	+	+/-	-	-	+	-	-	+	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119226]	12329	-	-	-	+	-	+	-	-	-	+	+	+	-	-	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5098]	Sample type/isolated from	human faeces, rectal swab
[Ref.: #5098]	Geographic location (country and/or sea, region)	Berlin
[Ref.: #5098]	Country	Germany
[Ref.: #5098]	Country ISO 3 Code	DEU
[Ref.: #5098]	Continent	Europe
[Ref.: #5098]	Geographic location	52.5186°/13.4083°

[Ref.: #119226]	Sample type/isolated from	Human, Faeces, rectal swab
* marker position based on {}

Isolation sources categories

#Infection	#Patient	#Swab
#Host	#Human	-
#Host Body-Site	#Gastrointestinal tract	#Rectum
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5098]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119226]	Biosafety level	2	Risk group (French classification)

Availability in culture collections External links

[Ref.: #5098]

Culture collection no.

DSM 13590, CIP 110055, WDCM 00154, CECT 8224, NCTC 13633

[Ref.: #74789]

SI-ID 143343

Literature:

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Sintered and 3D-Printed Bulks of MgB2-Based Materials with Antimicrobial Properties.	Badica P, Batalu ND, Chifiriuc MC, Burdusel M, Grigoroscuta MA, Aldica GV, Pasuk I, Kuncser A, Popa M, Agostino A, Operti L, Padhi SK, Bonino V, Truccato M	Molecules	10.3390/molecules26196045	2021	*
Pathogenicity	Comparison of different beta-lactam-glycopeptide-gentamicin combinations for an experimental endocarditis caused by a highly beta-lactam-resistant and highly glycopeptide-resistant isolate of Enterococcus faecium.	Caron F, Pestel M, Kitzis MD, Lemeland JF, Humbert G, Gutmann L	J Infect Dis	10.1093/infdis/171.1.106	1995	*
	Effect of Ottoman Viper (Montivipera xanthina (Gray, 1849)) Venom on Various Cancer Cells and on Microorganisms.	Yalcin HT, Ozen MO, Gocmen B, Nalbantsoy A	Cytotechnology	10.1007/s10616-013-9540-z	2013	*

References

#5098

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13590

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#36873 ; Curators of the CIP;

#68381

Automatically annotated from API rID32STR .

#68382

Automatically annotated from API zym .

#74789

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID143343.1 )

#119226 Collection of Institut Pasteur ; Curators of the CIP; CIP 110055
* These data were automatically processed and therefore are not curated

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