Strain identifier

BacDive ID: 5303

Type strain: No

Species: Enterococcus faecium

Strain Designation: U0317

Strain history: <- W. van Schalk, University Medical Center Utrecht, Dept. of Med. Microbiol., The Netherlands; U0317

NCBI tax ID(s): 1352 (species)

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General

@ref: 18027

BacDive-ID: 5303

DSM-Number: 25390

keywords: genome sequence, Bacteria, microaerophile

description: Enterococcus faecium U0317 is a microaerophile bacterium that was isolated from clinical isolate .

NCBI tax id

  • NCBI tax id: 1352
  • Matching level: species

strain history

  • @ref: 18027
  • history: <- W. van Schalk, University Medical Center Utrecht, Dept. of Med. Microbiol., The Netherlands; U0317

doi: 10.13145/bacdive5303.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus faecium
  • full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

  • synonyms

    • @ref: 20215
    • synonym: Streptococcus faecium

@ref: 18027

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus faecium

full scientific name: Enterococcus faecium (Orla-Jensen 1919) Schleifer and Kilpper-Bälz 1984

strain designation: U0317

type strain: no

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 91.304

colony morphology

  • @ref: 18027
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18027COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
18027TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 18027
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

oxygen tolerance

  • @ref: 18027
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837028087glycogen-builds acid from
6837015443inulin-builds acid from
6837027082trehalose+builds acid from
6837017716lactose+builds acid from
6837016899D-mannitol+builds acid from
6837030849L-arabinose+builds acid from
6837016988D-ribose+builds acid from
6837029016arginine+hydrolysis
683704853esculin+hydrolysis
68370606565hippurate+hydrolysis
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
683816731melezitose-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837015688acetoinyes
6838115688acetoinyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
6838115688acetoin+
6837015688acetoin+

enzymes

@refvalueactivityec
18027catalase-1.11.1.6
18027cytochrome-c oxidase-1.9.3.1
68381urease-3.5.1.5
68381glycyl tryptophan arylamidase+
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase+3.2.1.21
68381arginine dihydrolase+3.5.3.6
68370arginine dihydrolase+3.5.3.6
68370leucine arylamidase-3.4.11.1
68370alkaline phosphatase-3.1.3.1
68370beta-galactosidase+3.2.1.23
68370beta-glucuronidase-3.2.1.31
68370beta-glucosidase+3.2.1.21

API 20STR

@refVPHIPESCPYRAalpha GALbeta GURbeta GALPALLAPADHRIBARAMANSORLACTREINURAFAMDGLYG
18027+++---+--++++-++-++/--
18027+++++/--+--+++++/-++--+/--

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
18027+++-+-++-+++++-++-+++++--++-+-+-
18027+++-+-++-+++++-++-+++++--++-+-+-
18027+++-+-++-++-++--+-+-+/-++--+----+/--

Isolation, sampling and environmental information

isolation

  • @ref: 18027
  • sample type: clinical isolate (urinary tract infection)
  • geographic location: Utrecht
  • country: Netherlands
  • origin.country: NLD
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Medical environment#Clinic
#Host Body-Site#Urogenital tract

Safety information

risk assessment

  • @ref: 18027
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus faecium U0317GCA_000172915contigncbi547468
66792Enterococcus faecium U0317547468.4wgspatric547468
66792Enterococcus faecium U0317647000248draftimg547468

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes91.304no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no89.681yes
69480spore-formingspore-formingAbility to form endo- or exosporesno77.984no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no96.643no
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno97.5yes
69480flagellatedmotile2+Ability to perform flagellated movementno87no

External links

@ref: 18027

culture collection no.: DSM 25390

straininfo link

  • @ref: 74793
  • straininfo: 405106

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22024569Chemical structure of wall teichoic acid isolated from Enterococcus faecium strain U0317.Bychowska A, Theilacker C, Czerwicka M, Marszewska K, Huebner J, Holst O, Stepnowski P, Kaczynski ZCarbohydr Res10.1016/j.carres.2011.09.0262011Carbohydrate Conformation, Carbohydrate Sequence, Cell Wall/*chemistry, *Enterococcus faecium, Lipopolysaccharides/*chemistry/isolation & purification, Magnetic Resonance Spectroscopy, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Teichoic Acids/*chemistry/isolation & purificationEnzymology
Pathogenicity26611826Identification and functional characterization of the putative polysaccharide biosynthesis protein (CapD) of Enterococcus faecium U0317.Ali L, Spiess M, Wobser D, Rodriguez M, Blum HE, Sakiotanc TInfect Genet Evol10.1016/j.meegid.2015.11.0202015Animals, Bacterial Adhesion, Bacterial Capsules/chemistry/*genetics/metabolism, Caco-2 Cells, Cell Line, Disease Models, Animal, Enterococcus faecium/*growth & development/isolation & purification/pathogenicity, Gram-Positive Bacterial Infections/*microbiology, Humans, Hydrophobic and Hydrophilic Interactions, Mice, Mutation, Polysaccharides/*biosynthesisMetabolism
Pathogenicity31230165Detection and characterization of bacterial polysaccharides in drug-resistant enterococci.Ali L, Blum HE, Sakiotanc TGlycoconj J10.1007/s10719-019-09881-32019Animals, Anti-Bacterial Agents/pharmacology, Antibodies, Bacterial/biosynthesis/*pharmacology, Antigens, Bacterial/chemistry/immunology/*isolation & purification, Bacterial Capsules/chemistry/immunology, Cell Wall/*chemistry/immunology, Disease Models, Animal, Drug Resistance, Bacterial, Endocarditis, Bacterial/drug therapy/microbiology, Enterococcus faecium/*chemistry/drug effects/immunology/pathogenicity, Female, Gram-Positive Bacterial Infections/drug therapy/microbiology, Humans, Leukocytes, Mononuclear/cytology/drug effects/immunology, Lipopolysaccharides/chemistry/immunology/*isolation & purification/pharmacology, Mice, Mice, Inbred BALB C, Opsonin Proteins/pharmacology, Penicillin-Binding Proteins/chemistry/immunology/isolation & purification/pharmacology, Phagocytosis/drug effects, Polysaccharides, Bacterial/chemistry/immunology/*isolation & purification, Primary Cell Culture, Rats, Rats, Wistar, Teichoic Acids/chemistry/immunology/*isolation & purification/pharmacology, Urinary Tract Infections/drug therapy/microbiologyEnzymology

Reference

@idauthorscataloguedoi/urltitle
18027Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25390)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25390
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68370Automatically annotated from API 20STR
68381Automatically annotated from API rID32STR
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
74793Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID405106.1StrainInfo: A central database for resolving microbial strain identifiers