Acetobacter pasteurianus (DSM 3509, ATCC 33445, LMD 22.1)

Cell morphology

Multimedia

• @ref:	1403
  multimedia content:	DSM_3509.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_3509.jpg
  caption:	Micrograph of DSM 3509. Scale bar represents 2 um.
  intellectual property rights:	Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
38091	MEDIUM 1 - for Acetobacter, Azotobacter, Gluconobacter, Gluconacetobacter, Mesorhizodium ciceri and Pseudomonas doudoroffii			Distilled water make up to (1000.000 ml);Agar (15.000 g);Yeast extract (5.000 g);Peptone (3.000 g);Mannitol (25.000 g)
1403	GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105)		Medium recipe at MediaDive	Name: GLUCONOBACTER OXYDANS MEDIUM (DSMZ Medium 105) Composition: Glucose 100.0 g/l CaCO3 20.0 g/l Agar 15.0 g/l Yeast extract 10.0 g/l Distilled water
1403	YPM MEDIUM (DSMZ Medium 360)		Medium recipe at MediaDive	Name: YPM MEDIUM (DSMZ Medium 360) Composition: Mannitol 25.0 g/l Agar 12.0 g/l Yeast extract 5.0 g/l Peptone 3.0 g/l Distilled water
121620	CIP Medium 1		Medium recipe at CIP

Culture temp

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
22886	17790 ChEBI	methanol	-	growth

Enzymes

@ref	Value	Activity	Ec
22886	catalase	+	1.11.1.6

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM28527v1 assembly for Acetobacter pasteurianus subsp. pasteurianus LMG 1262 = NBRC 106471 type strain:LMG 1262	scaffold	GCA_000285275	940265.4	2548876776	940265	45.33
67770	ASM385080v1 assembly for Acetobacter pasteurianus subsp. pasteurianus LMG 1262 = NBRC 106471	contig	GCA_003850805	940265.9	2915977242	940265	35.07
67770	ASM333215v1 assembly for Acetobacter pasteurianus BCRC14145	contig	GCA_003332155	438.30		438	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Acetobacter pasteurianus 16S rRNA, partial sequence	AB003975	155		940265
20218	Acetobacter pasteurianus strain DSM 3509 16S ribosomal RNA gene, partial sequence	GQ240636	1336		940265
20218	Acetobacter pasteurianus 16S rRNA, partial sequence	AB003973	155		438
20218	Acetobacter pasteurianus strain LMG 1262 16S ribosomal RNA gene, partial sequence	GQ240637	1363		940265
20218	Acetobacter pasteurianus subsp. pasteurianus LMG 1262 16S ribosomal RNA gene, partial sequence	JF793964	1351		940265
20218	Acetobacter pasteurianus gene for 16S rRNA, partial sequence, strain: NBRC 3191	AB680026	1413		438
20218	Acetobacter pasteurianus 16S rRNA, partial sequence	AB003974	154		438
67770	A.pasteurianus gene for 16S ribosomal RNA	X71863	1479		940265
124043	Acetobacter pasteurianus gene for 16S rRNA, partial sequence, strain: NBRC 106471.	AB682450	1413		438

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Exploration of Lactiplantibacillus fabifermentans and Furfurilactobacillus rossiae as potential cocoa fermentation starters.	Korcari D, Ricci G, Fanton A, Emide D, Barbiroli A, Fortina MG.	J Appl Microbiol	10.1111/jam.15687	2022
Metabolism	Succession of selected strains of Acetobacter pasteurianus and other acetic acid bacteria in traditional balsamic vinegar.	Gullo M, De Vero L, Giudici P.	Appl Environ Microbiol	10.1128/aem.02249-08	2009
Metabolism	An in vitro synthetic biosystem based on acetate for production of phloroglucinol.	Zhang R, Liu W, Cao Y, Xu X, Xian M, Liu H.	BMC Biotechnol	10.1186/s12896-017-0376-z	2017
Pathogenicity	Putative ABC transporter responsible for acetic acid resistance in Acetobacter aceti.	Nakano S, Fukaya M, Horinouchi S.	Appl Environ Microbiol	10.1128/aem.72.1.497-505.2006	2006
Pathogenicity	Comparative evaluation of the genomes of three common Drosophila-associated bacteria.	Petkau K, Fast D, Duggal A, Foley E.	Biol Open	10.1242/bio.017673	2016
Metabolism	Characterization of acetic acid bacteria in traditional acetic acid fermentation of rice vinegar (komesu) and unpolished rice vinegar (kurosu) produced in Japan.	Nanda K, Taniguchi M, Ujike S, Ishihara N, Mori H, Ono H, Murooka Y.	Appl Environ Microbiol	10.1128/aem.67.2.986-990.2001	2001
Enzymology	Coffea arabica L., a new host plant for Acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria.	Jimenez-Salgado T, Fuentes-Ramirez LE, Tapia-Hernandez A, Mascarua-Esparza MA, Martinez-Romero E, Caballero-Mellado J.	Appl Environ Microbiol	10.1128/aem.63.9.3676-3683.1997	1997
	Comprehensive deciphering prophages in genus Acetobacter on the ecology, genomic features, toxin-antitoxin system, and linkage with CRISPR-Cas system.	Qian C, Ma J, Liang J, Zhang L, Liang X.	Front Microbiol	10.3389/fmicb.2022.951030	2022
	Recent advances of fermented fruits: A review on strains, fermentation strategies, and functional activities.	Yuan X, Wang T, Sun L, Qiao Z, Pan H, Zhong Y, Zhuang Y.	Food Chem X	10.1016/j.fochx.2024.101482	2024
Metabolism	Oral administration with a traditional fermented multi-fruit beverage modulates non-specific and antigen-specific immune responses in BALB/c mice.	Sy JBA, Hsu TC, Limaye A, Liu JR.	PLoS One	10.1371/journal.pone.0233047	2020
Biotechnology	Metagenomics analysis of cocoa bean fermentation microbiome identifying species diversity and putative functional capabilities.	Agyirifo DS, Wamalwa M, Otwe EP, Galyuon I, Runo S, Takrama J, Ngeranwa J.	Heliyon	10.1016/j.heliyon.2019.e02170	2019
	Chemical profiling of fungal metabolites via the OSMAC approach: Novel identification of Brianthein W from an endophytic fungus, Hypomontagnella monticulosa Zg15SU.	Lutfia A, Munir E, Yurnaliza Y, Basyuni M, Oku H.	Curr Res Microb Sci	10.1016/j.crmicr.2024.100288	2024
	Regulatory mechanisms of acetic acid, ethanol and high temperature tolerances of acetic acid bacteria during vinegar production.	Hua S, Wang Y, Wang L, Zhou Q, Li Z, Liu P, Wang K, Zhu Y, Han D, Yu Y.	Microb Cell Fact	10.1186/s12934-024-02602-y	2024
Metabolism	Bacterial Methionine Metabolism Genes Influence Drosophila melanogaster Starvation Resistance.	Judd AM, Matthews MK, Hughes R, Veloz M, Sexton CE, Chaston JM.	Appl Environ Microbiol	10.1128/aem.00662-18	2018
Metabolism	The utilization of sugar cane molasses with/without the presence of lignosulfonate for the production of bacterial cellulose.	Keshk S, Sameshima K.	Appl Microbiol Biotechnol	10.1007/s00253-005-0265-6	2006
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Metabolism	Whole-Genome Sequence Analysis of Bombella intestini LMG 28161T, a Novel Acetic Acid Bacterium Isolated from the Crop of a Red-Tailed Bumble Bee, Bombus lapidarius.	Li L, Illeghems K, Van Kerrebroeck S, Borremans W, Cleenwerck I, Smagghe G, De Vuyst L, Vandamme P.	PLoS One	10.1371/journal.pone.0165611	2016
Phylogeny	Classification of acetic acid bacteria and their acid resistant mechanism.	Qiu X, Zhang Y, Hong H.	AMB Express	10.1186/s13568-021-01189-6	2021
Genetics	Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus.	Azuma Y, Hosoyama A, Matsutani M, Furuya N, Horikawa H, Harada T, Hirakawa H, Kuhara S, Matsushita K, Fujita N, Shirai M.	Nucleic Acids Res	10.1093/nar/gkp612	2009
	Nickel-resistance determinants in Acidiphilium sp. PM identified by genome-wide functional screening.	San Martin-Uriz P, Mirete S, Alcolea PJ, Gomez MJ, Amils R, Gonzalez-Pastor JE.	PLoS One	10.1371/journal.pone.0095041	2014
	Flagellar Genes Are Associated with the Colonization Persistence Phenotype of the Drosophila melanogaster Microbiota.	Morgan SJ, Chaston JM.	Microbiol Spectr	10.1128/spectrum.04585-22	2023
Genetics	Comparative Genomic Analysis of Closely Related Acetobacter pasteurianus Strains Provides Evidence of Horizontal Gene Transfer and Reveals Factors Necessary for Thermotolerance.	Matsutani M, Matsumoto N, Hirakawa H, Shiwa Y, Yoshikawa H, Okamoto-Kainuma A, Ishikawa M, Kataoka N, Yakushi T, Matsushita K.	J Bacteriol	10.1128/jb.00553-19	2020
	Bacterial Metabolism and Transport Genes Are Associated with the Preference of Drosophila melanogaster for Dietary Yeast.	Call TB, Davis EK, Bean JD, Lemmon SG, Chaston JM.	Appl Environ Microbiol	10.1128/aem.00720-22	2022
Genetics	Genetic Influences of the Microbiota on the Life Span of Drosophila melanogaster.	Matthews MK, Wilcox H, Hughes R, Veloz M, Hammer A, Banks B, Walters A, Schneider KJ, Sexton CE, Chaston JM.	Appl Environ Microbiol	10.1128/aem.00305-20	2020
Genetics	In vivo function and comparative genomic analyses of the Drosophila gut microbiota identify candidate symbiosis factors.	Newell PD, Chaston JM, Wang Y, Winans NJ, Sannino DR, Wong AC, Dobson AJ, Kagle J, Douglas AE.	Front Microbiol	10.3389/fmicb.2014.00576	2014
Metabolism	The Peculiar Structure of Acetobacter pasteurianus CIP103108 LPS Core Oligosaccharide.	Marchetti R, Nieto Fabregat F, Pallach M, Gully D, Giraud E, Molinaro A, Duda KA, Silipo A	Chembiochem	10.1002/cbic.202000597	2020
Metabolism	Cobalamin is produced by Acetobacter pasteurianus DSM 3509.	Bernhardt C, Zhu X, Schutz D, Fischer M, Bisping B	Appl Microbiol Biotechnol	10.1007/s00253-019-09704-3	2019
Pathogenicity	Structure and inflammatory activity of the LPS isolated from Acetobacter pasteurianus CIP103108.	Pallach M, Di Lorenzo F, Facchini FA, Gully D, Giraud E, Peri F, Duda KA, Molinaro A, Silipo A	Int J Biol Macromol	10.1016/j.ijbiomac.2018.08.035	2018
Proteome	Proteome analysis of Acetobacter pasteurianus during acetic acid fermentation.	Andres-Barrao C, Saad MM, Chappuis ML, Boffa M, Perret X, Ortega Perez R, Barja F	J Proteomics	10.1016/j.jprot.2011.11.027	2011
Phylogeny	Numerical Analysis of Phenotypic Features and Protein Gel Electrophoregrams of a Wide Variety of Acetobacter strains. Proposal for the Improvement of the Taxonomy of the Genus Acetobacter Beijerinck 1898, 215.	Gossele F, Swings J, Kersters K, Pauwels P, De Ley J	Syst Appl Microbiol	10.1016/S0723-2020(83)80020-4	1983
Phylogeny	Acetobacter oryzoeni sp. nov., isolated from Korean rice wine vinegar.	Baek JH, Kim KH, Moon JY, Yeo SH, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijsem.0.004008	2020