Name: Ligilactobacillus salivarius H066
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6665

Type strain:

Species: Ligilactobacillus salivarius

Strain Designation: H066

Culture col. no.: CCUG 31453, LMG 9477, DSM 20555, ATCC 11741, NCDO 929, JCM 1231, BCRC 14759, CECT 4063, CIP 103140, NCFB 2747, NCIMB 11975, NRRL B-1949

Strain history: ATCC 11741 <-- M. Rogosa HO66.

NCBI tax ID(s): 1624 (species)

Other strains from Ligilactobacillus salivarius

Section

Ligilactobacillus salivarius H066 is a mesophilic bacterium that was isolated from saliva.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Ligilactobacillus*
	Species	**Ligilactobacillus salivarius**
	Full Scientific Name (PNU)	Ligilactobacillus salivarius (Rogosa et al. 1953) Zheng et al. 2020

	Synonym	Lactobacillus salivarius subsp. salicinius
	Synonym	Lactobacillus salivarius

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %96.629

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8905]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8905]

Culture medium growth

[Ref.: #8905]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #8905]

Culture medium composition

expand / minimize

[Ref.: #40537]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #40537]

Culture medium growth

[Ref.: #40537]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8905]	growth	37 °C
[Ref.: #40537]	growth	37 °C
[Ref.: #50608]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #8905]	Temperature range	mesophilic
[Ref.: #40537]	Temperature range	mesophilic
[Ref.: #50608]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.911

[Ref.: #8905]	Murein short key	A11.31
[Ref.: #8905]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #50608]

Fatty acid	Percentage	ECL
C16:0	26.2	16
C18:1 ω9c	21	17.769
C18:1 ω7c /12t/9t	19.3	17.824
C19:0 CYCLO ω9c	17.2	18.87
C14:0	5.4	14
C16:1 ω7c	4.4	15.819
Unidentified	2.7
C18:0	2	18
Unidentified	1.9

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8905]	8608	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	+	-	-	+	+	-	-	+	-	-	-	-	-	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #8905]	8352	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	+	+	-	-	+	-	-	-	-	-	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #8905]	8351	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	+/-	-	-	+	+	-	-	+	-	-	-	-	-	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #8905]	8350	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	+	-	-	+	+	-	-	+	-	-	-	-	-	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8905]	Sample type/isolated from	saliva

[Ref.: #50608]	Sample type/isolated from	Saliva

[Ref.: #67770]	Sample type/isolated from	Human saliva
* marker position based on {}

Isolation sources categories

#Host Body Product

#Oral cavity and Airways

#Saliva

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC071821 (>99% sequence identity) for Ligilactobacillus salivarius from Microbeatlas

Aquatic Samples	3859
Soil Samples	1629
Animal Samples	69457
Plant Samples	834
Total Samples	75779

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8905]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus salivarius gene for 16S ribosomal RNA, partial sequence, strain: JCM 1231	LC071821	1472	ENA	1624 tax ID
[Ref.: #20218]	Lactobacillus salivarius strain ATCC 11741 16S ribosomal RNA gene, complete sequence	AF089108	1570	ENA	1624 tax ID	*
[Ref.: #20218]	Lactobacillus salivarius subsp. salivarius 16S-23S ribosomal RNA intergenic spacer region, complete sequence	AF113600	210	ENA	1624 tax ID	*
[Ref.: #20218]	Lactobacillus salivarius strain DSM 20555 16S ribosomal RNA gene, partial sequence	DQ901733	1063	ENA	1624 tax ID	*
[Ref.: #20218]	Lactobacillus salivarius subsp. salivarius strain DSM 20555 16S ribosomal RNA gene, partial sequence	EF468102	575	ENA	1624 tax ID	*
[Ref.: #20218]	Lactobacillus salivarius subsp. salivarius gene for 16S rRNA, partial sequence, strain: JCM 1231	AB289296	657	ENA	1624 tax ID	*
[Ref.: #20218]	Lactobacillus salivarius gene for 16S ribosomal RNA, partial sequence, strain: JCM 1231	AB370881	1486	ENA	1624 tax ID	*
[Ref.: #20218]	Lactobacillus salivarius subsp. salivarius 16S ribosomal RNA, partial sequence; 16S/23S intergenic spacer region, complete sequence; and 23S ribosomal RNA partial sequence	AF182725	675	ENA	1624 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ligilactobacillus salivarius DSM 20555 = ATCC 11741 ATCC 11741	GCA_000159395	scaffold	GenBank	1423799 tax ID
[Ref.: #67770]	Ligilactobacillus salivarius DSM 20555 = ATCC 11741 DSM 20555	GCA_001435955	contig	GenBank	1423799 tax ID
[Ref.: #66792]	Lactobacillus salivarius DSM 20555 = ATCC 11741	1423799.3	wgs	PATRIC	1423799 tax ID	*
[Ref.: #66792]	Ligilactobacillus salivarius LMG 9477	GCA_029823545.1	contig	GenBank	1624 tax ID	*
[Ref.: #66792]	Lactobacillus salivarius strain BCRC 14759	1624.127	plasmid	PATRIC	1624 tax ID	*
[Ref.: #66792]	Lactobacillus salivarius strain BCRC 14759	1624.129	plasmid	PATRIC	1624 tax ID	*

[Ref.: #8905]

GC-content

34.7 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus salivarius strain BCRC 14759 PATRIC: 1624.113
[Ref.: #69480]	gram-positive	yes	100	yes
[Ref.: #69480]	spore-forming	no	99.911	no
[Ref.: #69480]	anaerobic	no	98.577	no
[Ref.: #69480]	thermophile	no	99.23	yes
[Ref.: #69480]	motile	no	96.629	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Lactobacillus salivarius strain BCRC 14759 PATRIC: 1624.113
[Ref.: #69481]	spore-forming	no	63	no

Availability in culture collections External links

[Ref.: #8905]

Culture collection no.

CCUG 31453, LMG 9477, DSM 20555, ATCC 11741, NCDO 929, JCM 1231, BCRC 14759, CECT 4063, CIP 103140, NCFB 2747, NCIMB 11975, NRRL B-1949

[Ref.: #20218]

Associated Passport(s) in StrainInfo

14125, 14124, 137837

Literature:

Only first 10 entries are displayed. Click here to see all.

	Title	Authors	Journal	DOI	Year
Pathogenicity	Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans.	Wasfi R, Abd El-Rahman OA, Zafer MM, Ashour HM	J Cell Mol Med	10.1111/jcmm.13496	2018	*
Metabolism	Evasin-displaying lactic acid bacteria bind different chemokines and neutralize CXCL8 production in Caco-2 cells.	Skrlec K, Pucer Janez A, Rogelj B, Strukelj B, Berlec A	Microb Biotechnol	10.1111/1751-7915.12781	2017	*
Metabolism	Inhibition of Fusarium solani Infection in Murine Keratocytes by Lactobacillus salivarius ssp. salivarius JCM1231 Culture Filtrate In Vitro.	Hu J, Chen F, Kan T, Zhuang H, Zhang J, Han X	Curr Eye Res	10.1080/02713683.2017.1317816	2017	*
Phylogeny	Heterologous surface display on lactic acid bacteria: non-GMO alternative?	Zadravec P, Strukelj B, Berlec A	Bioengineered	10.1080/21655979.2015.1040956	2015	*
Metabolism	Improvement of LysM-mediated surface display of designed ankyrin repeat proteins (DARPins) in recombinant and nonrecombinant strains of Lactococcus lactis and Lactobacillus Species.	Zadravec P, Strukelj B, Berlec A	Appl Environ Microbiol	10.1128/AEM.03694-14	2015	*
Metabolism	Antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens.	Chen PW, Jheng TT, Shyu CL, Mao FC	J Dairy Sci	10.3168/jds.2012-6112	2013	*
Pathogenicity	Protective effect of Lactobacillus casei strain Shirota against lethal infection with multi-drug resistant Salmonella enterica serovar Typhimurium DT104 in mice.	Asahara T, Shimizu K, Takada T, Kado S, Yuki N, Morotomi M, Tanaka R, Nomoto K	J Appl Microbiol	10.1111/j.1365-2672.2010.04884.x	2010	*
Metabolism	Immobilization of Lactobacillus salivarius ATCC 11741 on loofa sponge coated with chitosan for lactic acid fermentation.	Chantawongvuti R, Veerajetbodithat J, Jaturapiree P, Muangnapoh C	J Microbiol Biotechnol	JMB020-01-15	2010	*
Phylogeny	Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds.	Morita H, Shiratori C, Murakami M, Takami H, Kato Y, Endo A, Nakajima F, Takagi M, Akita H, Okada S, Masaoka T	Int J Syst Evol Microbiol	10.1099/ijs.0.65135-0	2007	*
	Lactobacillus plantarum Disrupts S. mutans-C. albicans Cross-Kingdom Biofilms.	Zeng Y, Fadaak A, Alomeir N, Wu TT, Rustchenko E, Qing S, Bao J, Gilbert C, Xiao J	Front Cell Infect Microbiol	10.3389/fcimb.2022.872012	2022	*
	Antioxidants Bioaccessibility and Lactobacillus salivarius (CECT 4063) Survival Following the In Vitro Digestion of Vacuum Impregnated Apple Slices: Effect of the Drying Technique, the Addition of Trehalose, and High-Pressure Homogenization.	Burca-Busaga CG, Betoret N, Segui L, Garcia-Hernandez J, Hernandez M, Barrera C	Foods	10.3390/foods10092155	2021	*
	Survival of Lactobacillus salivarius CECT 4063 and Stability of Antioxidant Compounds in Dried Apple Snacks as Affected by the Water Activity, the Addition of Trehalose and High Pressure Homogenization.	Burca-Busaga CG, Betoret N, Segui L, Betoret E, Barrera C	Microorganisms	10.3390/microorganisms8081095	2020	*
	Microbial Community and FermentationDynamics of Corn Silage Prepared withHeat-Resistant Lactic Acid Bacteria in a HotEnvironment.	Guan H, Shuai Y, Yan Y, Ran Q, Wang X, Li D, Cai Y, Zhang X	Microorganisms	10.3390/microorganisms8050719	2020	*
	Microencapsulation of functional strains by high pressure homogenization for a potential use in fermented milk.	Patrignani F, Siroli L, Serrazanetti DI, Braschi G, Betoret E, Reinheimer JA, Lanciotti R	Food Res Int	10.1016/j.foodres.2017.04.020	2017	*
	Whole-Genome Sequencing of Lactobacillus salivarius Strains BCRC 14759 and BCRC 12574.	Chiu SH, Chen CC, Wang LT, Huang L	Genome Announc	10.1128/genomeA.01336-17	2017	*
	Characterization of lactic acid bacteria isolated from the poultry intestinal environment with anti-Salmonella activity in vitro.	Hidalgo VM, Babot JD, Fernandez MM, Perez Chaia A, Audisio C, Apella MC	Braz J Microbiol	10.1007/s42770-022-00860-9	2022	*

References

#8905

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20555

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40537 ; Curators of the CIP;
#50608 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 31453

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Search for species Ligilactobacillus salivarius in external resources:
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Lactobacillus salivarius strain BCRC 14759

Lactobacillus salivarius strain BCRC 14759

Change proposal

Change proposal