Name: Amylolactobacillus amylotrophicus DSM 20534
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6666

Type strain:

Species: Amylolactobacillus amylotrophicus

Culture col. no.: DSM 20534, LMG 11400, NRRL B-4436, JCM 1124, BCRC 17923, CIP 109878, KCTC 3160

Strain history: CIP <- 2008, JCM <- S.M. Naser, Ghent Univ., Ghent, Belgium <- NRRL B-4436 <- L.K. Nakamura: strain 1124

NCBI tax ID(s): 1423722 (strain), 376807 (species)

SI-ID 264383

LMG 11400, DSM 20534, JCM 1124, NRRL B-4436, KCTC 3160, CIP 109878

Special Collections

Section

Amylolactobacillus amylotrophicus DSM 20534 is a facultative anaerobe, Gram-positive bacterium that produces lactate and was isolated from swine waste-corn fermentation.

lactate production
Gram-positive
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Amylolactobacillus*
	Species	**Amylolactobacillus amylotrophicus**
	Full Scientific Name (LPSN)	Amylolactobacillus amylotrophicus (Naser et al. 2006) Zheng et al. 2020

Synonym

Lactobacillus amylotrophicus

Information on morphological and physiological properties Morphology

[Ref.: #116362]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %95.19

[Ref.: #116362]	Cell shape	rod-shaped

[Ref.: #116362]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %90.833

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8764]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8764]

Culture medium growth

[Ref.: #8764]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8764]

Culture medium composition

expand / minimize

[Ref.: #37866]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #37866]

Culture medium growth

[Ref.: #37866]

Culture medium composition

expand / minimize

[Ref.: #116362]

Culture medium

CIP Medium 40

[Ref.: #116362]

Culture medium growth

[Ref.: #116362]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #8764]		growth	28 °C
[Ref.: #37866]		growth	37 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #116362]		growth	15-37 °C
[Ref.: #116362]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116362]

Oxygen tolerance

facultative anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

[Ref.: #8764]

Name of produced compound

L(+) lactic acid

[Ref.: #8764]	Murein short key	A11.31
[Ref.: #8764]	Murein types	A4alpha L-Lys-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #116362]	nitrate ChEBI	-	reduction
[Ref.: #116362]	nitrate ChEBI	+	respiration
[Ref.: #116362]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #116362]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116362]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116362]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116362]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116362]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116362]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8764]	14692	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	+	+	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-	-	-	-	-
[Ref.: #116362]	11682	not determinedn.d.	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+/-	+/-	+	+	-	-	-	-	-	-	-	+	+	-	+	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116362]	12185	-	+	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8764]	Sample type/isolated from	swine waste-corn fermentation

[Ref.: #67770]	Sample type/isolated from	Swine waste-corn fermentation

[Ref.: #116362]	Sample type/isolated from	Swine waste-corn fermentation
[Ref.: #116362]	Geographic location (country and/or sea, region)	Ghent
[Ref.: #116362]	Country	Belgium
[Ref.: #116362]	Country ISO 3 Code	BEL
[Ref.: #116362]	Continent	Europe
[Ref.: #116362]	Isolation date	2006
* marker position based on {}

Isolation sources categories

#Engineered	#Waste	-
#Host	#Mammals	#Suidae (Pig,Swine)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM236149 (>99% sequence identity) for Amylolactobacillus from Microbeatlas

Aquatic Samples	188
Soil Samples	145
Animal Samples	930
Plant Samples	71
Total Samples	1334

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8764]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116362]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus amylotrophicus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1124	LC064890	1476	GenBank ENA	376807 tax ID
[Ref.: #20218]	Lactobacillus iners partial 16S rRNA gene, type strain CIP 109878T	HE573916	1560	GenBank ENA	147802 tax ID	*
[Ref.: #20218]	Lactobacillus amylotrophicus partial 16S rRNA gene, type strain CIP 109878T	HE573917	1560	GenBank ENA	376807 tax ID	*
[Ref.: #20218]	Lactobacillus amylotrophicus gene for 16S rRNA, partial sequence, strain: JCM 1124	AB289027	676	GenBank ENA	376807 tax ID	*
[Ref.: #20218]	Lactobacillus amylotrophicus 16S rRNA gene, strain type strain: LMG 11400T	AM236149	1488	GenBank ENA	376807 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Amylolactobacillus amylotrophicus DSM 20534	GCA_001434555	scaffold	GenBank ENA	1423722 tax ID
[Ref.: #66792]	Lactobacillus amylotrophicus DSM 20534	1423722.3	wgs	PATRIC	1423722 tax ID	*
[Ref.: #66792]	Amylolactobacillus amylotrophicus DSM 20534	2695420818	draft	JGI IMG/M	1423722 tax ID	*

[Ref.: #8764]	GC-content	45.5 mol%
[Ref.: #67770]	GC-content	43.5 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Amylolactobacillus amylotrophicus DSM 20534 DSM 20534 NCBI: GCA_001434555.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus amylotrophicus DSM 20534 PATRIC: 1423722.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	95.19	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	85.331	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	88.267	no
[Ref.: #69480]	aerobic	aerobicⓘ	no	95.692	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	90.833	no

Availability in culture collections External links

[Ref.: #8764]

Culture collection no.

DSM 20534, LMG 11400, NRRL B-4436, JCM 1124, BCRC 17923, CIP 109878, KCTC 3160

[Ref.: #76140]

SI-ID 264383

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of Lactobacillus amylophilus LMG 11400 and NRRL B-4435 as Lactobacillus amylotrophicus sp. nov.	Naser SM, Vancanneyt M, Snauwaert C, Vrancken G, Hoste B, De Vuyst L, Swings J	Int J Syst Evol Microbiol	10.1099/ijs.0.64463-0	2006	*

References

#8764

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20534

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37866 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76140

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID264383.1 )

#116362 Collection of Institut Pasteur ; Curators of the CIP; CIP 109878
* These data were automatically processed and therefore are not curated

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Amylolactobacillus amylotrophicus DSM 20534 DSM 20534

Lactobacillus amylotrophicus DSM 20534

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