Strain identifier

BacDive ID: 6665

Type strain: Yes

Species: Ligilactobacillus salivarius

Strain Designation: H066, HO 66

Strain history: CIP <- 1988, DSMZ <- ATCC <- M. Rogosa: strain HO 66

NCBI tax ID(s): 1624 (species)

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General

@ref: 8905

BacDive-ID: 6665

DSM-Number: 20555

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic

description: Ligilactobacillus salivarius H066 is a facultative anaerobe, mesophilic bacterium that was isolated from saliva.

NCBI tax id

  • NCBI tax id: 1624
  • Matching level: species

strain history

@refhistory
8905<- ATCC <- M. Rogosa, H066
67770ATCC 11741 <-- M. Rogosa HO66.
122389CIP <- 1988, DSMZ <- ATCC <- M. Rogosa: strain HO 66

doi: 10.13145/bacdive6665.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Ligilactobacillus
  • species: Ligilactobacillus salivarius
  • full scientific name: Ligilactobacillus salivarius (Rogosa et al. 1953) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus salivarius subsp. salicinius
    20215Lactobacillus salivarius

@ref: 8905

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Ligilactobacillus

species: Ligilactobacillus salivarius

full scientific name: Ligilactobacillus salivarius (Rogosa et al. 1953) Zheng et al. 2020

strain designation: H066, HO 66

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.629
69480100positive
122389nopositiverod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8905MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
40537MEDIUM 40- for Lactobacillus and LeuconostocyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
122389CIP Medium 40yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=40

culture temp

@refgrowthtypetemperaturerange
8905positivegrowth37mesophilic
40537positivegrowth37mesophilic
50608positivegrowth37mesophilic
67770positivegrowth37mesophilic
122389positivegrowth22-45
122389nogrowth10psychrophilic
122389nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 122389
  • oxygen tolerance: facultative anaerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.911

murein

  • @ref: 8905
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose+builds acid from17716
68371maltose+builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol+builds acid from17924
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose+builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122389nitrate-reduction17632
122389nitrite-reduction16301
122389nitrate+respiration17632

metabolite tests

  • @ref: 122389
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase+3.1.3.1
122389oxidase-
122389alcohol dehydrogenase-1.1.1.1
122389catalase-1.11.1.6
122389lysine decarboxylase-4.1.1.18
122389ornithine decarboxylase-4.1.1.17

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    50608C14:05.414
    50608C16:026.216
    50608C18:0218
    50608C16:1 ω7c4.415.819
    50608C18:1 ω7c /12t/9t19.317.824
    50608C18:1 ω9c2117.769
    50608C19:0 CYCLO ω9c17.218.87
    50608Unidentified1.9
    50608Unidentified2.7
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122389-+---+----+-++------

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8905----------++++-+--++--+-----+++++--+--------------
8905----------++++----++--+-----+++++--+--------------
8905----------++++-+/---++--+-----+++++--+--------------
8905----------++++-+--++--+-----+++++--+--------------

Isolation, sampling and environmental information

isolation

@refsample type
8905saliva
50608Saliva
67770Human saliva
122389Human, Saliva

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Oral cavity and Airways
  • Cat3: #Saliva

taxonmaps

  • @ref: 69479
  • File name: preview.99_595.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_379;97_427;98_487;99_595&stattab=map
  • Last taxonomy: Ligilactobacillus salivarius
  • 16S sequence: LC071821
  • Sequence Identity:
  • Total samples: 75779
  • soil counts: 1629
  • aquatic counts: 3859
  • animal counts: 69457
  • plant counts: 834

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
89051Risk group (German classification)
1223891Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus salivarius strain ATCC 11741 16S ribosomal RNA gene, complete sequenceAF0891081570ena1624
20218Lactobacillus salivarius subsp. salivarius 16S-23S ribosomal RNA intergenic spacer region, complete sequenceAF113600210ena1624
20218Lactobacillus salivarius strain DSM 20555 16S ribosomal RNA gene, partial sequenceDQ9017331063ena1624
20218Lactobacillus salivarius subsp. salivarius strain DSM 20555 16S ribosomal RNA gene, partial sequenceEF468102575ena1624
20218Lactobacillus salivarius subsp. salivarius gene for 16S rRNA, partial sequence, strain: JCM 1231AB289296657ena1624
20218Lactobacillus salivarius gene for 16S ribosomal RNA, partial sequence, strain: JCM 1231AB3708811486ena1624
20218Lactobacillus salivarius subsp. salivarius 16S ribosomal RNA, partial sequence; 16S/23S intergenic spacer region, complete sequence; and 23S ribosomal RNA partial sequenceAF182725675ena1624
67770Lactobacillus salivarius gene for 16S ribosomal RNA, partial sequence, strain: JCM 1231LC0718211472ena1624

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ligilactobacillus salivarius BCRC 14759GCA_002736025chromosomencbi1624
66792Lactobacillus salivarius ATCC 11741525364.3wgspatric1423799
66792Lactobacillus salivarius DSM 20555 = ATCC 117411423799.3wgspatric1423799
66792Lactobacillus salivarius strain BCRC 147591624.128plasmidpatric1624
66792Lactobacillus salivarius strain BCRC 147591624.127plasmidpatric1624
66792Lactobacillus salivarius strain BCRC 147591624.129plasmidpatric1624
66792Lactobacillus salivarius strain BCRC 147591624.113completepatric1624
66792Ligilactobacillus salivarius BCRC 147592898118425completeimg1624
66792Ligilactobacillus salivarius DSM 205552663763409draftimg1423799
66792Ligilactobacillus salivarius HO66, ATCC 11741643886048draftimg1423799
67770Ligilactobacillus salivarius DSM 20555 = ATCC 11741GCA_000159395scaffoldncbi1423799
67770Ligilactobacillus salivarius DSM 20555 = ATCC 11741GCA_001435955contigncbi1423799
66792Ligilactobacillus salivarius LMG 9477GCA_029823545contigncbi1624

GC content

  • @ref: 8905
  • GC-content: 34.7

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno63no
motileno95.638no
gram-positiveyes95.288no
anaerobicno65.011no
aerobicno96.952no
halophileyes81.828no
spore-formingno94.986no
thermophileno97.863yes
glucose-utilyes91.738no
flagellatedno98.252no
glucose-fermentyes88.256no

External links

@ref: 8905

culture collection no.: CCUG 31453, LMG 9477, DSM 20555, ATCC 11741, NCDO 929, JCM 1231, BCRC 14759, CECT 4063, CIP 103140, NCFB 2747, NCIMB 11975, NRRL B-1949, NCDO 2747

straininfo link

  • @ref: 76139
  • straininfo: 3366

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18048734Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds.Morita H, Shiratori C, Murakami M, Takami H, Kato Y, Endo A, Nakajima F, Takagi M, Akita H, Okada S, Masaoka TInt J Syst Evol Microbiol10.1099/ijs.0.65135-02007Aerobiosis/physiology, Anaerobiosis/physiology, Animals, Bacterial Typing Techniques, Base Composition, Catalase/analysis, DNA/chemistry/genetics, DNA, Bacterial/chemistry/genetics, Feces/*microbiology, Fermentation, Genes, rRNA, Glucose/metabolism, Horses, Japan, Lactic Acid/metabolism, Lactobacillus/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Spores, Bacterial/cytologyMetabolism
Metabolism20134241Immobilization of Lactobacillus salivarius ATCC 11741 on loofa sponge coated with chitosan for lactic acid fermentation.Chantawongvuti R, Veerajetbodithat J, Jaturapiree P, Muangnapoh CJ Microbiol BiotechnolJMB020-01-152010Cells, Immobilized/chemistry/metabolism, Chitosan/chemistry, *Fermentation, Industrial Microbiology/*methods, Lactic Acid/*metabolism, Lactobacillus/chemistry/*metabolismBiotechnology
Pathogenicity21059159Protective effect of Lactobacillus casei strain Shirota against lethal infection with multi-drug resistant Salmonella enterica serovar Typhimurium DT104 in mice.Asahara T, Shimizu K, Takada T, Kado S, Yuki N, Morotomi M, Tanaka R, Nomoto KJ Appl Microbiol10.1111/j.1365-2672.2010.04884.x2010Acetic Acid/pharmacology, Animals, Disease Models, Animal, Drug Resistance, Multiple, Bacterial, Lactobacillus, *Lactobacillus casei, Male, Mice, Mice, Inbred BALB C, Probiotics/pharmacology/*therapeutic use, Salmonella Infections, Animal/pathology/*prevention & control, *Salmonella typhimurium/drug effects/growth & development
Metabolism23332852Antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens.Chen PW, Jheng TT, Shyu CL, Mao FCJ Dairy Sci10.3168/jds.2012-61122013Animals, Anti-Infective Agents/administration & dosage/*therapeutic use, Bifidobacterium/metabolism, Cattle, Enterococcus faecalis/drug effects, Escherichia coli/drug effects, Lactobacillus/metabolism, Lactobacillus acidophilus/metabolism, Lactobacillus rhamnosus/metabolism, Lactoferrin/administration & dosage/drug effects/*therapeutic use, Pediococcus/metabolism, Probiotics/administration & dosage/*therapeutic use, Protein Hydrolysates, Salmonella typhimurium/drug effectsPathogenicity
Metabolism25576617Improvement of LysM-mediated surface display of designed ankyrin repeat proteins (DARPins) in recombinant and nonrecombinant strains of Lactococcus lactis and Lactobacillus Species.Zadravec P, Strukelj B, Berlec AAppl Environ Microbiol10.1128/AEM.03694-142015*Ankyrin Repeat, Cell Surface Display Techniques/*methods, Cell Wall/metabolism, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Lactobacillus/genetics/*metabolism, Lactococcus lactis/genetics/*metabolism, Microscopy, Fluorescence, Peptidoglycan/metabolism, Recombinant Fusion Proteins/genetics/*metabolism
Phylogeny25880164Heterologous surface display on lactic acid bacteria: non-GMO alternative?Zadravec P, Strukelj B, Berlec ABioengineered10.1080/21655979.2015.10409562015Cell Surface Display Techniques/*methods, Cell Wall/*chemistry, Lactobacillus/*chemistry/classification, Organisms, Genetically Modified, Recombinant Fusion Proteins/*chemistry
28578048Microencapsulation of functional strains by high pressure homogenization for a potential use in fermented milk.Patrignani F, Siroli L, Serrazanetti DI, Braschi G, Betoret E, Reinheimer JA, Lanciotti RFood Res Int10.1016/j.foodres.2017.04.0202017Cultured Milk Products/*microbiology, Drug Compounding/*methods, Food Handling/*methods, Food Storage, Lactobacillus/chemistry/*physiology, Microbial Viability, Models, Biological, Polysaccharides, Bacterial/analysis, Pressure, *Probiotics
Metabolism28636459Inhibition of Fusarium solani Infection in Murine Keratocytes by Lactobacillus salivarius ssp. salivarius JCM1231 Culture Filtrate In Vitro.Hu J, Chen F, Kan T, Zhuang H, Zhang J, Han XCurr Eye Res10.1080/02713683.2017.13178162017Animals, *Antibiosis/physiology, Cell Line, Chromatography, High Pressure Liquid, Corneal Keratocytes/*microbiology, Enzyme-Linked Immunosorbent Assay, Eye Infections, Fungal/prevention & control, Flow Cytometry, Fumonisins/metabolism, Fusariosis/prevention & control, Fusarium/*growth & development, Interleukin-6/genetics/metabolism, Lactobacillus salivarius/*physiology, Mice, Microbial Sensitivity Tests, Real-Time Polymerase Chain Reaction, Tandem Mass Spectrometry, Tumor Necrosis Factor-alpha/genetics/metabolism, Zearalenone/metabolismProteome
Metabolism28736998Evasin-displaying lactic acid bacteria bind different chemokines and neutralize CXCL8 production in Caco-2 cells.Skrlec K, Pucer Janez A, Rogelj B, Strukelj B, Berlec AMicrob Biotechnol10.1111/1751-7915.127812017Caco-2 Cells, Chemokines, CXC/*metabolism, Gene Expression, Humans, Interleukin-8/*metabolism, Intestinal Mucosa/*metabolism, Intestines/microbiology, Lactococcus lactis/genetics/*metabolism, Protein Binding, Receptors, CXCR/*genetics/metabolismPathogenicity
29167259Whole-Genome Sequencing of Lactobacillus salivarius Strains BCRC 14759 and BCRC 12574.Chiu SH, Chen CC, Wang LT, Huang LGenome Announc10.1128/genomeA.01336-172017
Pathogenicity29316223Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries-inducing Streptococcus mutans.Wasfi R, Abd El-Rahman OA, Zafer MM, Ashour HMJ Cell Mol Med10.1111/jcmm.134962018*Antibiosis, Bacteriocins/pharmacology, Biofilms/*drug effects/growth & development, Catalase/pharmacology, Culture Media/chemistry, Humans, Hydrogen-Ion Concentration, Interferon-gamma/biosynthesis, Interleukin-10/biosynthesis, Lactobacillus casei/drug effects/growth & development/metabolism, Lactobacillus plantarum/drug effects/growth & development/metabolism, Lactobacillus reuteri/drug effects/growth & development/metabolism, Lactobacillus salivarius/drug effects/*growth & development/metabolism, Leukocytes, Mononuclear/cytology/immunology/microbiology, Microbial Sensitivity Tests, Peroxides/pharmacology, Polysaccharides, Bacterial/antagonists & inhibitors/biosynthesis, Primary Cell Culture, Probiotics/*pharmacology, Quorum Sensing/drug effects, Streptococcus mutans/*drug effects/genetics/growth & development/pathogenicity, Trypsin/pharmacologyMetabolism
32408707Microbial Community and FermentationDynamics of Corn Silage Prepared withHeat-Resistant Lactic Acid Bacteria in a HotEnvironment.Guan H, Shuai Y, Yan Y, Ran Q, Wang X, Li D, Cai Y, Zhang XMicroorganisms10.3390/microorganisms80507192020
32707848Survival of Lactobacillus salivarius CECT 4063 and Stability of Antioxidant Compounds in Dried Apple Snacks as Affected by the Water Activity, the Addition of Trehalose and High Pressure Homogenization.Burca-Busaga CG, Betoret N, Segui L, Betoret E, Barrera CMicroorganisms10.3390/microorganisms80810952020
34574265Antioxidants Bioaccessibility and Lactobacillus salivarius (CECT 4063) Survival Following the In Vitro Digestion of Vacuum Impregnated Apple Slices: Effect of the Drying Technique, the Addition of Trehalose, and High-Pressure Homogenization.Burca-Busaga CG, Betoret N, Segui L, Garcia-Hernandez J, Hernandez M, Barrera CFoods10.3390/foods100921552021
35392605Lactobacillus plantarum Disrupts S. mutans-C. albicans Cross-Kingdom Biofilms.Zeng Y, Fadaak A, Alomeir N, Wu TT, Rustchenko E, Qing S, Bao J, Gilbert C, Xiao JFront Cell Infect Microbiol10.3389/fcimb.2022.8720122022Biofilms, Candida albicans/physiology, *Dental Caries, *Lactobacillus plantarum, Streptococcus mutans/genetics
36333643Characterization of lactic acid bacteria isolated from the poultry intestinal environment with anti-Salmonella activity in vitro.Hidalgo VM, Babot JD, Fernandez MM, Perez Chaia A, Audisio C, Apella MCBraz J Microbiol10.1007/s42770-022-00860-92022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8905Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20555)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20555
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40537Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14905
50608Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31453)https://www.ccug.se/strain?id=31453
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
76139Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3366.1StrainInfo: A central database for resolving microbial strain identifiers
122389Curators of the CIPCollection of Institut Pasteur (CIP 103140)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103140