Cupriavidus necator (CCUG 52966, LMG 1200, ATCC 23442)

Name: Cupriavidus necator (CCUG 52966, LMG 1200, ATCC 23442)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1998

Type strain

Strain Designation H 1

Culture col. no. CCUG 52966 LMG 1200 ATCC 23442 DSM 529 ATCC 17698 1 more

NCBI tax ID(s) 106590

Special Collections DSMZ CCUG

History DSMZ <- IMG, H 1 <- R. Bartha

Links

Cupriavidus necator H 1 is an aerobe bacterium that was isolated from sludge from a garden pond.

aerobe Bacteria

Name and taxonomic classification

SI-ID 699

LMG 1200,ATCC 17698,ATCC 23442,DSM 529,KCTC 2337,CCUG 52966

@ref 20215

Last LPSN update 2026-05-29 08:25:23

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Cupriavidus

Species Cupriavidus necator

Full scientific name Cupriavidus necator Makkar and Casida 1987

BacDive ID	Other strains from **Cupriavidus necator** (39)	Type strain
2033	C. necator N-1, Jr N-1, DSM 13513, ATCC 43291, CCUG ... (type strain)
330	C. necator 1978, DSM 2625, CCUG 52964, ATCC 33178, LMG ...
1999	C. necator H 20, DSM 530, ATCC 17700
2001	C. necator H 16 G+1, H 16 G⁺1, DSM 538
2002	C. necator H 16 F 34, DSM 539
2003	C. necator H 16 F 29, DSM 540
2004	C. necator H 16 PHB-4, H 16 PHB^-4, DSM 541
2005	C. necator LH-7, LH^-7, DSM 416
2006	C. necator PH-9, PH^-9, DSM 418
2007	C. necator PH-4, PH^-4, DSM 422
2008	C. necator pHG1, H 16, H16, Wilde H16, strain S-10-1, ...
2009	C. necator H16 25/1, DSM 430
2010	C. necator B 19, DSM 515, JCM 20894, CCUG 13796, IAM ...
2011	C. necator G 27, DSM 516
2012	C. necator G 29, DSM 517
2013	C. necator DSM 518
2014	C. necator H 16 G+7, H 16 G⁺7, DSM 542
2015	C. necator G 29 G+1, G 29 G⁺1, DSM 543
2016	C. necator H 20 G+3, H 20 G⁺3, DSM 544
2017	C. necator H 1 G+3, H 1 G⁺3, DSM 545
2018	C. necator H 1 G+4, H 1 G⁺4, DSM 546
2019	C. necator H 20 G+1, H 20 G⁺1, DSM 547
2020	C. necator H 16 G+3, H 16 G⁺3, DSM 551
2021	C. necator H16 pHS4, DSM 3102
2022	C. necator JMP363 Smr, pJP2, DSM 4056
2023	C. necator JMP364, pJP3, JMP364 Smr, DSM 4057
2024	C. necator JMP134, pJP4, DSM 4058, NCIB 12592
2025	C. necator JMP365, pJP5, JMP365 Smr, DSM 4059
2026	C. necator JMP366, pJP7, JMP366 Smr, DSM 4060
2027	C. necator TA06, DSM 4182
2028	C. necator H850, DSM 5536, NRRL B-15940
2031	C. necator Bo, Tfa 17, DSM 11098
2032	C. necator H850-4LT, DSM 13439
2034	C. necator C145, DSM 15443
2035	C. necator HF39, DSM 15444
2036	C. necator DSM 30029, ATCC 25207, NRRL B-2804, CCUG ...
162984	C. necator JCM 20695, IAM 12628
163005	C. necator JCM 20908, ATCC 17702, IAM 13548, LMG 1203
163006	C. necator JCM 20909, ATCC 17708, IAM 13549, LMG 1208

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
127	MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)		Medium recipe at MediaDive	Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water
127	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
127	positive	growth	30
59753	positive	growth	30-37

Physiology and metabolism

Oxygen tolerance

59753

Oxygen toleranceaerobe

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

59753

Fatty acid	Percentage	ECL
C16:0	31.4	16
C16:1 ω7c	27.3	15.819
C17:0 CYCLO	12.2	16.888
C18:1 ω7c /12t/9t	11.4	17.824
C14:0 3OH/C16:1 ISO I	5.2	15.485
C14:0	4.5	14
C12:0 ALDE ?	2.5	10.915
C14:0 2OH	2.3	15.205
C16:1 2OH	0.7	17.047
C18:1 2OH	0.6	19.088
C16:0 2OH	0.5	17.233
C16:1 ω5c	0.4	15.908
C15:0	0.4	15
C18:2 ω6,9c/C18:0 ANTE	0.3	17.724
C18:0	0.2	18
Unidentified	0.2	18.135
unknown 14.503	0.2	14.503

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
59753	-	+	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
59753	+	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+	-	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Garden
#Environmental	#Terrestrial	#Mud (Sludge)
#Environmental	#Aquatic	#Pond (small)

Isolation

@ref	Sample type	Sampling date	Country	Country ISO 3 Code	Continent
127	sludge from a garden pond
59753	Sludge,garden pond	1962	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
127	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization.	Miu DM, Eremia MC, Moscovici M.	Materials (Basel)	10.3390/ma15041410	2022
	Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity.	Blunt W, Levin DB, Cicek N.	Polymers (Basel)	10.3390/polym10111197	2018
Metabolism	Microbial synthesis of 3-deoxy-D-erythro-hex-2-ulosonic acid 6-phosphate.	Knappmann BR, el-Nawawy MA, Schlegel HG, Kula MR	Carbohydr Res	10.1016/0008-6215(93)80029-e	1993
Enzymology	Molecular and genetic characterization of an Alcaligenes eutrophus insertion element.	Kung SS, Chen J, Chow WY	J Bacteriol	10.1128/jb.174.24.8023-8029.1992	1992

References

#127	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 529
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#59753	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 52966
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Cupriavidus necator (CCUG 52966, LMG 1200, ATCC 23442)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome-based predictions

Literature

Literature

References

BacDive

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