Name: Cupriavidus necator 1978
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 330

Type strain:

Species: Cupriavidus necator

Strain Designation: 1978

Culture col. no.: DSM 2625, CCUG 52964, ATCC 33178, LMG 1190

Strain history: <- H.G. Schlegel <- K. Kersters <- K. Ohi (Alcaligenes hydrogenophilus)

NCBI tax ID(s): 512 (species)

SI-ID 689

LMG 1190, ATCC 33178, DSM 2625, KCTC 2338, BCRC 12881, CCRC 12881, CCUG 52964

Other strains from Cupriavidus necator

C. necator N-1, Jr N-1, DSM 13513, ATCC 43291, ... (type strain)
C. necator H 1, CCUG 52966, LMG 1200, ATCC ...
C. necator H 20, DSM 530, ATCC 17700
C. necator H 16 G+1, H 16 G⁺1, ...
C. necator H 16 F 34, DSM 539
C. necator H 16 F 29, DSM 540
C. necator H 16 PHB-4, H 16 PHB^-4, ...
C. necator LH-7, LH^-7, DSM 416
C. necator PH-9, PH^-9, DSM 418
C. necator PH-4, PH^-4, DSM 422
C. necator pHG1, H 16, H16, DSM 428, ATCC ...
C. necator H16 25/1, DSM 430
C. necator B 19, DSM 515, JCM 20894, CCUG ...
C. necator G 27, DSM 516
C. necator G 29, DSM 517
C. necator DSM 518
C. necator H 16 G+7, H 16 G⁺7, ...
C. necator G 29 G+1, G 29 G⁺1, ...
C. necator H 20 G+3, H 20 G⁺3, ...
C. necator H 1 G+3, H 1 G⁺3, DSM ...
C. necator H 1 G+4, H 1 G⁺4, DSM ...
C. necator H 20 G+1, H 20 G⁺1, ...
C. necator H 16 G+3, H 16 G⁺3, ...
C. necator H16 pHS4, DSM 3102
C. necator JMP363 Smr, pJP2, DSM 4056
C. necator JMP364, pJP3, JMP364 Smr, DSM 4057
C. necator JMP134, pJP4, DSM 4058, NCIB 12592
C. necator JMP365, pJP5, JMP365 Smr, DSM 4059
C. necator JMP366, pJP7, JMP366 Smr, DSM 4060
C. necator TA06, DSM 4182
C. necator H850, DSM 5536, NRRL B-15940
C. necator Bo, Tfa 17, DSM 11098
C. necator H850-4LT, DSM 13439
C. necator C145, DSM 15443
C. necator HF39, DSM 15444
C. necator DSM 30029, ATCC 25207, NRRL B-2804, ...
C. necator STI05003(IMET), pS3,
C. necator JCM 20695, IAM 12628
C. necator JCM 20908, ATCC 17702, IAM 13548, ...
C. necator JCM 20909, ATCC 17708, IAM 13549, ...

Section

Cupriavidus necator 1978 is an aerobe, mesophilic bacterium that was isolated from paddy field soil.

aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Cupriavidus*
	Species	**Cupriavidus necator**
	Full Scientific Name (LPSN)	Cupriavidus necator Makkar and Casida 1987

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1172]

Culture medium

MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)

[Ref.: #1172]

Culture medium growth

[Ref.: #1172]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1172]

Culture medium composition

expand / minimize

[Ref.: #1172]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #1172]

Culture medium growth

[Ref.: #1172]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1172]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #1172]	growth	28 °C
[Ref.: #1172]	growth	30 °C
[Ref.: #59752]	growth	30-37 °C

[Ref.: #1172]	Temperature range	mesophilic
[Ref.: #59752]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #59752]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #59752]

Fatty acid	Percentage	ECL
C16:1 ω7c	34.3	15.819
C16:0	25.5	16
C18:1 ω7c /12t/9t	21	17.824
C14:0 3OH/C16:1 ISO I	8.3	15.485
C14:0	2.8	14
C17:0 CYCLO	2.8	16.888
C14:0 2OH	2.1	15.205
C16:1 2OH	0.8	17.047
C18:1 2OH	0.7	19.088
C16:1 ω5c	0.3	15.908
C18:0	0.3	18
C17:0	0.3	17
C15:0	0.3	15
Unidentified	0.3	17.224
C18:1 ω9c	0.2	17.769
C20:0	0.1	20
C18:2 ω6,9c/C18:0 ANTE	0.1	17.724

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #59752]	8371	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #59752]	4307	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1172]	Sample type/isolated from	paddy field soil
[Ref.: #1172]	Geographic location (country and/or sea, region)	Osaka
[Ref.: #1172]	Country	Japan
[Ref.: #1172]	Country ISO 3 Code	JPN
[Ref.: #1172]	Continent	Asia

[Ref.: #59752]	Sample type/isolated from	Soil,paddy field
[Ref.: #59752]	Country	Japan
[Ref.: #59752]	Country ISO 3 Code	JPN
[Ref.: #59752]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Condition	#Anoxic (anaerobic)	-
#Condition	#Humid	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1172]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Alcaligenes hydrogenophilus strain DSM 2625 16S ribosomal RNA gene, partial sequence	JF271921	607	ENA	516 tax ID	*

[Ref.: #1172]

GC-content

70.2 mol%

Availability in culture collections External links

[Ref.: #1172]

Culture collection no.

DSM 2625, CCUG 52964, ATCC 33178, LMG 1190

[Ref.: #70011]

SI-ID 689

References

#1172

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 2625

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#59752 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 52964

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#70011

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID689.1 )

* These data were automatically processed and therefore are not curated

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