Cupriavidus necator (DSM 541, H 16 PHB-4, H 16 PHB^-4)

Name: Cupriavidus necator (DSM 541, H 16 PHB-4, H 16 PHB - 4)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2004

Type strain

Strain Designation H 16 PHB-4, H 16 PHB^-4

Culture col. no. DSM 541 H 16 PHB-4 H 16 PHB^-4

NCBI tax ID(s) 106590

Special Collections DSMZ

History DSMZ <- IMG <- H.G. Schlegel, H 16 PHB^-4

Links

Cupriavidus necator H 16 PHB-4 is a bacterium of the family Burkholderiaceae.

Bacteria

Name and taxonomic classification

SI-ID 47208

DSM 541

@ref 20215

Last LPSN update 2026-05-29 08:25:23

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Cupriavidus

Species Cupriavidus necator

Full scientific name Cupriavidus necator Makkar and Casida 1987

BacDive ID	Other strains from **Cupriavidus necator** (39)	Type strain
2033	C. necator N-1, Jr N-1, DSM 13513, ATCC 43291, CCUG ... (type strain)
330	C. necator 1978, DSM 2625, CCUG 52964, ATCC 33178, LMG ...
1998	C. necator H 1, CCUG 52966, LMG 1200, ATCC 23442, DSM ...
1999	C. necator H 20, DSM 530, ATCC 17700
2001	C. necator H 16 G+1, H 16 G⁺1, DSM 538
2002	C. necator H 16 F 34, DSM 539
2003	C. necator H 16 F 29, DSM 540
2005	C. necator LH-7, LH^-7, DSM 416
2006	C. necator PH-9, PH^-9, DSM 418
2007	C. necator PH-4, PH^-4, DSM 422
2008	C. necator pHG1, H 16, H16, Wilde H16, strain S-10-1, ...
2009	C. necator H16 25/1, DSM 430
2010	C. necator B 19, DSM 515, JCM 20894, CCUG 13796, IAM ...
2011	C. necator G 27, DSM 516
2012	C. necator G 29, DSM 517
2013	C. necator DSM 518
2014	C. necator H 16 G+7, H 16 G⁺7, DSM 542
2015	C. necator G 29 G+1, G 29 G⁺1, DSM 543
2016	C. necator H 20 G+3, H 20 G⁺3, DSM 544
2017	C. necator H 1 G+3, H 1 G⁺3, DSM 545
2018	C. necator H 1 G+4, H 1 G⁺4, DSM 546
2019	C. necator H 20 G+1, H 20 G⁺1, DSM 547
2020	C. necator H 16 G+3, H 16 G⁺3, DSM 551
2021	C. necator H16 pHS4, DSM 3102
2022	C. necator JMP363 Smr, pJP2, DSM 4056
2023	C. necator JMP364, pJP3, JMP364 Smr, DSM 4057
2024	C. necator JMP134, pJP4, DSM 4058, NCIB 12592
2025	C. necator JMP365, pJP5, JMP365 Smr, DSM 4059
2026	C. necator JMP366, pJP7, JMP366 Smr, DSM 4060
2027	C. necator TA06, DSM 4182
2028	C. necator H850, DSM 5536, NRRL B-15940
2031	C. necator Bo, Tfa 17, DSM 11098
2032	C. necator H850-4LT, DSM 13439
2034	C. necator C145, DSM 15443
2035	C. necator HF39, DSM 15444
2036	C. necator DSM 30029, ATCC 25207, NRRL B-2804, CCUG ...
162984	C. necator JCM 20695, IAM 12628
163005	C. necator JCM 20908, ATCC 17702, IAM 13548, LMG 1203
163006	C. necator JCM 20909, ATCC 17708, IAM 13549, LMG 1208

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
134	MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)		Medium recipe at MediaDive	Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water
134	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
134	positive	growth	28
134	positive	growth	30

Physiology and metabolism

Compound production

@ref	Compound
134	ribonuclease
134	ribulose-1,5-bisphosphate carboxylase
134	glycollate
134	single cell protein

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
134	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	CnRed: Efficient, Marker-free Genome Engineering of Cupriavidus necator H16 by Adapted Lambda Red Recombineering.	Arhar S, Pirchner J, Stolterfoht-Stock H, Reicher K, Kourist R, Emmerstorfer-Augustin A.	ACS Synth Biol	10.1021/acssynbio.4c00757	2025
	Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16.	Jang Y, Lee YJ, Gong G, Lee SM, Um Y, Kim KH, Ko JK.	Microb Cell Fact	10.1186/s12934-024-02398-x	2024
	CO₂-based production of phytase from highly stable expression plasmids in Cupriavidus necator H16.	Arhar S, Rauter T, Stolterfoht-Stock H, Lambauer V, Kratzer R, Winkler M, Karava M, Kourist R, Emmerstorfer-Augustin A.	Microb Cell Fact	10.1186/s12934-023-02280-2	2024
	Autotrophic Production of the Sesquiterpene alpha-Humulene with Cupriavidus necator in a Controlled Bioreactor.	Sydow A, Becker L, Lombard E, Ulber R, Guillouet SE, Holtmann D.	Bioengineering (Basel)	10.3390/bioengineering10101194	2023
	Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology.	Mullerova L, Markova K, Obruca S, Mravec F.	Microorganisms	10.3390/microorganisms10061179	2022
Metabolism	Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator.	Jahn M, Crang N, Janasch M, Hober A, Forsstrom B, Kimler K, Mattausch A, Chen Q, Asplund-Samuelsson J, Hudson EP.	Elife	10.7554/elife.69019	2021
	N-terminal truncation of PhaC_BP-M-CPF4 and its effect on PHA production.	Neoh SZ, Tan HT, Trakunjae C, Chek MF, Vaithanomsat P, Hakoshima T, Sudesh K.	Microb Cell Fact	10.1186/s12934-024-02329-w	2024
	Continuous Supply of Non-Combustible Gas Mixture for Safe Autotrophic Culture to Produce Polyhydroxyalkanoate by Hydrogen-Oxidizing Bacteria.	Miyahara Y, Wang CT, Ishii-Hyakutake M, Tsuge T.	Bioengineering (Basel)	10.3390/bioengineering9100586	2022
	Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol.	Gascoyne JL, Bommareddy RR, Heeb S, Malys N.	Metab Eng	10.1016/j.ymben.2021.06.010	2021
	Growth medium and electrolyte-How to combine the different requirements on the reaction solution in bioelectrochemical systems using Cupriavidus necator.	Sydow A, Krieg T, Ulber R, Holtmann D.	Eng Life Sci	10.1002/elsc.201600252	2017
Metabolism	Accumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing.	Obruca S, Sedlacek P, Krzyzanek V, Mravec F, Hrubanova K, Samek O, Kucera D, Benesova P, Marova I.	PLoS One	10.1371/journal.pone.0157778	2016
Metabolism	Carbon Sources for Polyhydroxyalkanoates and an Integrated Biorefinery.	Jiang G, Hill DJ, Kowalczuk M, Johnston B, Adamus G, Irorere V, Radecka I.	Int J Mol Sci	10.3390/ijms17071157	2016
	Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining.	Juengert JR, Bresan S, Jendrossek D.	Bio Protoc	10.21769/bioprotoc.2748	2018
	Bio-Based and Biodegradable Polymeric Materials for a Circular Economy.	Oliver-Cuenca V, Salaris V, Munoz-Gimena PF, Aguero A, Peltzer MA, Montero VA, Arrieta MP, Sempere-Torregrosa J, Pavon C, Samper MD, Crespo GR, Kenny JM, Lopez D, Peponi L.	Polymers (Basel)	10.3390/polym16213015	2024
Metabolism	A closer look on the polyhydroxybutyrate- (PHB-) negative phenotype of Ralstonia eutropha PHB-4.	Raberg M, Voigt B, Hecker M, Steinbuchel A.	PLoS One	10.1371/journal.pone.0095907	2014
Metabolism	Impact of multiple beta-ketothiolase deletion mutations in Ralstonia eutropha H16 on the composition of 3-mercaptopropionic acid-containing copolymers.	Lindenkamp N, Peplinski K, Volodina E, Ehrenreich A, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.01058-10	2010
Metabolism	Cloning and molecular analysis of the Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3.	Matsusaki H, Manji S, Taguchi K, Kato M, Fukui T, Doi Y.	J Bacteriol	10.1128/jb.180.24.6459-6467.1998	1998
Biotechnology	PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003.	Thomas T, Sudesh K, Bazire A, Elain A, Tan HT, Lim H, Bruzaud S	Bioengineering (Basel)	10.3390/bioengineering7010029	2020
Biotechnology	Monitoring poly(3-hydroxybutyrate) production in cupriavidus necator DSM 428 (H16) with raman spectroscopy.	Gelder JD, Willemse-Erix D, Scholtes MJ, Sanchez JI, Maquelin K, Vandenabeele P, Boever PD, Puppels GJ, Moens L, Vos PD	Anal Chem	10.1021/ac702185d	2008
Metabolism	Engineering of chimeric class II polyhydroxyalkanoate synthases.	Niamsiri N, Delamarre SC, Kim YR, Batt CA	Appl Environ Microbiol	10.1128/AEM.70.11.6789-6799.2004	2004
Enzymology	Sequence of PHA synthase gene from two strains of Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across two heterologous expression systems.	Clemente T, Shah D, Tran M, Stark D, Padgette S, Dennis D, Bruckener K, Steinbuchel A, Mitsky T	Appl Microbiol Biotechnol	10.1007/s002530051636	2000

References

#134	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 541
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Cupriavidus necator (DSM 541, H 16 PHB-4, H 16 PHB^-4)

Name and taxonomic classification

Morphology