Cupriavidus necator (DSM 4058, JMP134, NCIB 12592)

Name: Cupriavidus necator (DSM 4058, JMP134, NCIB 12592)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2024

Type strain

Strain Designation JMP134, pJP4

Culture col. no. DSM 4058 JMP134 NCIB 12592 pJP4

NCBI tax ID(s) 106590

Special Collections DSMZ

Links

Cupriavidus necator JMP134 is a bacterium of the family Burkholderiaceae.

Bacteria

Name and taxonomic classification

SI-ID 112642

DSM 4058,NCIMB 12592

@ref 20215

Last LPSN update 2026-05-29 08:25:23

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Cupriavidus

Species Cupriavidus necator

Full scientific name Cupriavidus necator Makkar and Casida 1987

BacDive ID	Other strains from **Cupriavidus necator** (39)	Type strain
2033	C. necator N-1, Jr N-1, DSM 13513, ATCC 43291, CCUG ... (type strain)
330	C. necator 1978, DSM 2625, CCUG 52964, ATCC 33178, LMG ...
1998	C. necator H 1, CCUG 52966, LMG 1200, ATCC 23442, DSM ...
1999	C. necator H 20, DSM 530, ATCC 17700
2001	C. necator H 16 G+1, H 16 G⁺1, DSM 538
2002	C. necator H 16 F 34, DSM 539
2003	C. necator H 16 F 29, DSM 540
2004	C. necator H 16 PHB-4, H 16 PHB^-4, DSM 541
2005	C. necator LH-7, LH^-7, DSM 416
2006	C. necator PH-9, PH^-9, DSM 418
2007	C. necator PH-4, PH^-4, DSM 422
2008	C. necator pHG1, H 16, H16, Wilde H16, strain S-10-1, ...
2009	C. necator H16 25/1, DSM 430
2010	C. necator B 19, DSM 515, JCM 20894, CCUG 13796, IAM ...
2011	C. necator G 27, DSM 516
2012	C. necator G 29, DSM 517
2013	C. necator DSM 518
2014	C. necator H 16 G+7, H 16 G⁺7, DSM 542
2015	C. necator G 29 G+1, G 29 G⁺1, DSM 543
2016	C. necator H 20 G+3, H 20 G⁺3, DSM 544
2017	C. necator H 1 G+3, H 1 G⁺3, DSM 545
2018	C. necator H 1 G+4, H 1 G⁺4, DSM 546
2019	C. necator H 20 G+1, H 20 G⁺1, DSM 547
2020	C. necator H 16 G+3, H 16 G⁺3, DSM 551
2021	C. necator H16 pHS4, DSM 3102
2022	C. necator JMP363 Smr, pJP2, DSM 4056
2023	C. necator JMP364, pJP3, JMP364 Smr, DSM 4057
2025	C. necator JMP365, pJP5, JMP365 Smr, DSM 4059
2026	C. necator JMP366, pJP7, JMP366 Smr, DSM 4060
2027	C. necator TA06, DSM 4182
2028	C. necator H850, DSM 5536, NRRL B-15940
2031	C. necator Bo, Tfa 17, DSM 11098
2032	C. necator H850-4LT, DSM 13439
2034	C. necator C145, DSM 15443
2035	C. necator HF39, DSM 15444
2036	C. necator DSM 30029, ATCC 25207, NRRL B-2804, CCUG ...
162984	C. necator JCM 20695, IAM 12628
163005	C. necator JCM 20908, ATCC 17702, IAM 13548, LMG 1203
163006	C. necator JCM 20909, ATCC 17708, IAM 13549, LMG 1208

Morphology

Culture and growth conditions

Physiology and metabolism

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1581	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass.	Volova TG, Kiselev EG, Demidenko AV, Zhila NO, Nemtsev IV, Lukyanenko AV.	Polymers (Basel)	10.3390/polym14010132	2021
	High Cell Density Cultivation of Paracoccus sp. on Sugarcane Juice for Poly(3-hydroxybutyrate) Production.	Moungprayoon A, Lunprom S, Reungsang A, Salakkam A.	Front Bioeng Biotechnol	10.3389/fbioe.2022.878688	2022
Phylogeny	Short-term direct contact with soil and plant materials leads to an immediate increase in diversity of skin microbiota.	Gronroos M, Parajuli A, Laitinen OH, Roslund MI, Vari HK, Hyoty H, Puhakka R, Sinkkonen A.	Microbiologyopen	10.1002/mbo3.645	2019
	Skin exposure to soil microbiota elicits changes in cell-mediated immunity to pneumococcal vaccine.	Roslund MI, Nurminen N, Oikarinen S, Puhakka R, Gronroos M, Puustinen L, Kummola L, Parajuli A, Cinek O, Laitinen OH, Hyoty H, Sinkkonen A.	Sci Rep	10.1038/s41598-024-68235-8	2024
	Indoor green wall affects health-associated commensal skin microbiota and enhances immune regulation: a randomized trial among urban office workers.	Soininen L, Roslund MI, Nurminen N, Puhakka R, Laitinen OH, Hyoty H, Sinkkonen A, ADELE research group.	Sci Rep	10.1038/s41598-022-10432-4	2022
	Meat and bone meal stimulates microbial diversity and suppresses plant pathogens in asparagus straw composting.	Liu X, Li X, Hua Y, Sinkkonen A, Romantschuk M, Lv Y, Wu Q, Hui N.	Front Microbiol	10.3389/fmicb.2022.953783	2022
	Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production.	Bedade DK, Edson CB, Gross RA.	Molecules	10.3390/molecules26113463	2021
Phylogeny	Habitat Elevation Shapes Microbial Community Composition and Alter the Metabolic Functions in Wild Sable (Martes zibellina) Guts.	Su L, Liu X, Jin G, Ma Y, Tan H, Khalid M, Romantschuk M, Yin S, Hui N.	Animals (Basel)	10.3390/ani11030865	2021
Metabolism	Homotaurine metabolized to 3-sulfopropanoate in Cupriavidus necator H16: enzymes and genes in a patchwork pathway.	Mayer J, Cook AM.	J Bacteriol	10.1128/jb.00678-09	2009
	Simulation of Microbial Response to Accidental Diesel Spills in Basins Containing Brackish Sea Water and Sediment.	Yan L, Hui N, Simpanen S, Tudeer L, Romantschuk M.	Front Microbiol	10.3389/fmicb.2020.593232	2020
Metabolism	Abundance of novel and diverse tfdA-like genes, encoding putative phenoxyalkanoic acid herbicide-degrading dioxygenases, in soil.	Zaprasis A, Liu YJ, Liu SJ, Drake HL, Horn MA.	Appl Environ Microbiol	10.1128/aem.01727-09	2010
	Urbanization Reduces Transfer of Diverse Environmental Microbiota Indoors.	Parajuli A, Gronroos M, Siter N, Puhakka R, Vari HK, Roslund MI, Jumpponen A, Nurminen N, Laitinen OH, Hyoty H, Rajaniemi J, Sinkkonen A.	Front Microbiol	10.3389/fmicb.2018.00084	2018
	Half-lives of PAHs and temporal microbiota changes in commonly used urban landscaping materials.	Roslund MI, Gronroos M, Rantalainen AL, Jumpponen A, Romantschuk M, Parajuli A, Hyoty H, Laitinen O, Sinkkonen A.	PeerJ	10.7717/peerj.4508	2018
	Biostimulation proved to be the most efficient method in the comparison of in situ soil remediation treatments after a simulated oil spill accident.	Simpanen S, Dahl M, Gerlach M, Mikkonen A, Malk V, Mikola J, Romantschuk M.	Environ Sci Pollut Res Int	10.1007/s11356-016-7606-0	2016
	Potential of preventive bioremediation to reduce environmental contamination by pesticides in an agricultural context: A case study with the herbicide 2,4-D.	Carles L, Martin-Laurent F, Devers M, Spor A, Rouard N, Beguet J, Besse-Hoggan P, Batisson I	J Hazard Mater	10.1016/j.jhazmat.2021.125740	2021
Metabolism	Efficient Degradation of Phenoxyalkanoic Acid Herbicides by the Alkali-Tolerant Cupriavidus oxalaticus Strain X32.	Xiang S, Lin R, Shang H, Xu Y, Zhang Z, Wu X, Zong F	J Agric Food Chem	10.1021/acs.jafc.9b05061	2020
Enzymology	Substrate Specificity in Thiol Dioxygenases.	Aloi S, Davies CG, Karplus PA, Wilbanks SM, Jameson GNL	Biochemistry	10.1021/acs.biochem.9b00079	2019
Metabolism	Siderophore-Mediated Iron Acquisition Enhances Resistance to Oxidative and Aromatic Compound Stress in Cupriavidus necator JMP134.	Li C, Zhu L, Pan D, Li S, Xiao H, Zhang Z, Shen X, Wang Y, Long M	Appl Environ Microbiol	10.1128/AEM.01938-18	2018
Metabolism	Biofilm vs. Planktonic Lifestyle: Consequences for Pesticide 2,4-D Metabolism by Cupriavidus necator JMP134.	Lerch TZ, Chenu C, Dignac MF, Barriuso E, Mariotti A	Front Microbiol	10.3389/fmicb.2017.00904	2017
Metabolism	Rapid Biodegradation of the Herbicide 2,4-Dichlorophenoxyacetic Acid by Cupriavidus gilardii T-1.	Wu X, Wang W, Liu J, Pan D, Tu X, Lv P, Wang Y, Cao H, Wang Y, Hua R	J Agric Food Chem	10.1021/acs.jafc.7b00544	2017
Metabolism	Simultaneous biodegradation of three mononitrophenol isomers by a tailor-made microbial consortium immobilized in sequential batch reactors.	Fu H, Zhang JJ, Xu Y, Chao HJ, Zhou NY	Lett Appl Microbiol	10.1111/lam.12696	2017
Enzymology	Cloning, expression, characterization and mutational analysis of the tfdA gene from Cupriavidus campinensis BJ71.	Han L, Liu Y, Li C, Zhao D	World J Microbiol Biotechnol	10.1007/s11274-015-1852-z	2015
Metabolism	Structures of the inducer-binding domain of pentachlorophenol-degrading gene regulator PcpR from Sphingobium chlorophenolicum.	Hayes RP, Moural TW, Lewis KM, Onofrei D, Xun L, Kang C	Int J Mol Sci	10.3390/ijms151120736	2014
Metabolism	Microbial degradation of 2,4-dichlorophenoxyacetic acid: Insight into the enzymes and catabolic genes involved, their regulation and biotechnological implications.	Kumar A, Trefault N, Olaniran AO	Crit Rev Microbiol	10.3109/1040841X.2014.917068	2014
Enzymology	Two structurally different dienelactone hydrolases (TfdEI and TfdEII) from Cupriavidus necator JMP134 plasmid pJP4 catalyse cis- and trans-dienelactones with similar efficiency.	Kumar A, Pillay B, Olaniran AO	PLoS One	10.1371/journal.pone.0101801	2014
Phylogeny	16S rRNA gene phylogeny and tfdA gene analysis of 2,4-D-degrading bacteria isolated in China.	Han L, Liu Y, He A, Zhao D	World J Microbiol Biotechnol	10.1007/s11274-014-1680-6	2014
Metabolism	Construction of an engineered strain capable of degrading two isomeric nitrophenols via a sacB- and gfp-based markerless integration system.	Hu F, Jiang X, Zhang JJ, Zhou NY	Appl Microbiol Biotechnol	10.1007/s00253-014-5567-0	2014
Enzymology	Plasmid-mediated bioaugmentation of sequencing batch reactors for enhancement of 2,4-dichlorophenoxyacetic acid removal in wastewater using plasmid pJP4.	Tsutsui H, Anami Y, Matsuda M, Hashimoto K, Inoue D, Sei K, Soda S, Ike M	Biodegradation	10.1007/s10532-012-9591-4	2012
Metabolism	Bioaugmentation with a consortium of bacterial nitrophenol-degraders for remediation of soil contaminated with three nitrophenol isomers.	Chi XQ, Zhang JJ, Zhao S, Zhou NY	Environ Pollut	10.1016/j.envpol.2012.08.002	2012
Metabolism	Structural and catalytic differences between two FADH(2)-dependent monooxygenases: 2,4,5-TCP 4-monooxygenase (TftD) from Burkholderia cepacia AC1100 and 2,4,6-TCP 4-monooxygenase (TcpA) from Cupriavidus necator JMP134.	Hayes RP, Webb BN, Subramanian AK, Nissen M, Popchock A, Xun L, Kang C	Int J Mol Sci	10.3390/ijms13089769	2012
Enzymology	A novel cold-adapted esterase from Salinisphaera sp. P7-4: gene cloning, overproduction, and characterization.	Kim YO, Park IS, Kim HK, Nam BH, Jeong Kong H, Kim WJ, Kim DG, Kim KK, Lee SJ	J Gen Appl Microbiol	10.2323/jgam.57.357	2011
Enzymology	Furfural reduction mechanism of a zinc-dependent alcohol dehydrogenase from Cupriavidus necator JMP134.	Kang C, Hayes R, Sanchez EJ, Webb BN, Li Q, Hooper T, Nissen MS, Xun L	Mol Microbiol	10.1111/j.1365-2958.2011.07914.x	2011
Metabolism	Effect of glucose on the fatty acid composition of Cupriavidus necator JMP134 during 2,4-dichlorophenoxyacetic acid degradation: implications for lipid-based stable isotope probing methods.	Lerch TZ, Dignac MF, Barriuso E, Mariotti A	Appl Environ Microbiol	10.1128/AEM.06438-11	2011
Enzymology	A non-NadB type L-aspartate dehydrogenase from Ralstonia eutropha strain JMP134: molecular characterization and physiological functions.	Li Y, Ishida M, Ashida H, Ishikawa T, Shibata H, Sawa Y	Biosci Biotechnol Biochem	10.1271/bbb.110216	2011
Enzymology	Cupriavidus necator JMP134 rapidly reduces furfural with a Zn-dependent alcohol dehydrogenase.	Li Q, Metthew Lam LK, Xun L	Biodegradation	10.1007/s10532-011-9476-y	2011
Enzymology	Biochemical characterization of ethanol-dependent reduction of furfural by alcohol dehydrogenases.	Li Q, Metthew Lam LK, Xun L	Biodegradation	10.1007/s10532-011-9477-x	2011
Metabolism	Strict and direct transcriptional repression of the pobA gene by benzoate avoids 4-hydroxybenzoate degradation in the pollutant degrader bacterium Cupriavidus necator JMP134.	Donoso RA, Perez-Pantoja D, Gonzalez B	Environ Microbiol	10.1111/j.1462-2920.2011.02470.x	2011
Enzymology	Characterization of catabolic meta-nitrophenol nitroreductase from Cupriavidus necator JMP134.	Yin Y, Xiao Y, Liu HZ, Hao F, Rayner S, Tang H, Zhou NY	Appl Microbiol Biotechnol	10.1007/s00253-010-2666-4	2010
Metabolism	Mineralization of PCBs by the genetically modified strain Cupriavidus necator JMS34 and its application for bioremediation of PCBs in soil.	Saavedra JM, Acevedo F, Gonzalez M, Seeger M	Appl Microbiol Biotechnol	10.1007/s00253-010-2575-6	2010
Metabolism	Characterization of MnpC, a hydroquinone dioxygenase likely involved in the meta-nitrophenol degradation by Cupriavidus necator JMP134.	Yin Y, Zhou NY	Curr Microbiol	10.1007/s00284-010-9640-3	2010
Genetics	The complete multipartite genome sequence of Cupriavidus necator JMP134, a versatile pollutant degrader.	Lykidis A, Perez-Pantoja D, Ledger T, Mavromatis K, Anderson IJ, Ivanova NN, Hooper SD, Lapidus A, Lucas S, Gonzalez B, Kyrpides NC	PLoS One	10.1371/journal.pone.0009729	2010
Enzymology	2,3-Dihydroxypropane-1-sulfonate degraded by Cupriavidus pinatubonensis JMP134: purification of dihydroxypropanesulfonate 3-dehydrogenase.	Mayer J, Huhn T, Habeck M, Denger K, Hollemeyer K, Cook AM	Microbiology (Reading)	10.1099/mic.0.037580-0	2010
Metabolism	A beta-barrel outer membrane protein facilitates cellular uptake of polychlorophenols in Cupriavidus necator.	Belchik SM, Schaeffer SM, Hasenoehrl S, Xun L	Biodegradation	10.1007/s10532-009-9313-8	2009
Enzymology	Crystal structure and catalytic mechanism of 4-methylmuconolactone methylisomerase.	Marin M, Heinz DW, Pieper DH, Klink BU	J Biol Chem	10.1074/jbc.M109.024604	2009
Metabolism	Involvement of several transcriptional regulators in the differential expression of tfd genes in Cupriavidus necator JMP134.	Trefault N, Guzman L, Perez H, Godoy M, Gonzalez B	Int Microbiol	im2306126	2009
Metabolism	Genuine genetic redundancy in maleylacetate-reductase-encoding genes involved in degradation of haloaromatic compounds by Cupriavidus necator JMP134.	Perez-Pantoja D, Donoso RA, Sanchez MA, Gonzalez B	Microbiology (Reading)	10.1099/mic.0.032086-0	2009
Metabolism	3-Chlorobenzoate is taken up by a chromosomally encoded transport system in Cupriavidus necator JMP134.	Ledger T, Aceituno F, Gonzalez B	Microbiology (Reading)	10.1099/mic.0.029207-0	2009
Metabolism	Microbial synthesis of poly((R)-3-hydroxybutyrate-co-3-hydroxypropionate) from unrelated carbon sources by engineered Cupriavidus necator.	Fukui T, Suzuki M, Tsuge T, Nakamura S	Biomacromolecules	10.1021/bm801391j	2009
Metabolism	Metabolic reconstruction of aromatic compounds degradation from the genome of the amazing pollutant-degrading bacterium Cupriavidus necator JMP134.	Perez-Pantoja D, De la Iglesia R, Pieper DH, Gonzalez B	FEMS Microbiol Rev	10.1111/j.1574-6976.2008.00122.x	2008
Enzymology	Secretion pathway for the poly(3-hydroxybutyrate) depolymerase in Ralstonia pickettii T1.	Sugimoto A, Shiraki M, Hatakeyama S, Saito T	Antonie Van Leeuwenhoek	10.1007/s10482-008-9235-1	2008
Metabolism	Functions of flavin reductase and quinone reductase in 2,4,6-trichlorophenol degradation by Cupriavidus necator JMP134.	Belchik SM, Xun L	J Bacteriol	10.1128/JB.01697-07	2007
Metabolism	Tracing 2,4-D metabolism in Cupriavidus necator JMP134 with 13C-labelling technique and fatty acid profiling.	Lerch TZ, Dignac MF, Barriuso E, Bardoux G, Mariotti A	J Microbiol Methods	10.1016/j.mimet.2007.08.003	2007
Metabolism	Genetic characterization of 2,4,6-trichlorophenol degradation in Cupriavidus necator JMP134.	Sanchez MA, Gonzalez B	Appl Environ Microbiol	10.1128/AEM.02584-06	2007
Phylogeny	Role of eukaryotic microbiota in soil survival and catabolic performance of the 2,4-D herbicide degrading bacteria Cupriavidus necator JMP134.	Manzano M, Moran AC, Tesser B, Gonzalez B	Antonie Van Leeuwenhoek	10.1007/s10482-006-9101-y	2007
Metabolism	Molecular and population analyses of a recombination event in the catabolic plasmid pJP4.	Larrain-Linton J, De la Iglesia R, Melo F, Gonzalez B	J Bacteriol	10.1128/JB.00869-06	2006
Metabolism	Chlorophenol hydroxylases encoded by plasmid pJP4 differentially contribute to chlorophenoxyacetic acid degradation.	Ledger T, Pieper DH, Gonzalez B	Appl Environ Microbiol	10.1128/AEM.72.4.2783-2792.2006	2006
Metabolism	Amino acids in positions 48, 52, and 73 differentiate the substrate specificities of the highly homologous chlorocatechol 1,2-dioxygenases CbnA and TcbC.	Liu S, Ogawa N, Senda T, Hasebe A, Miyashita K	J Bacteriol	10.1128/JB.187.15.5427-5436.2005	2005
Metabolism	A previously unexposed forest soil microbial community degrades high levels of the pollutant 2,4,6-trichlorophenol.	Sanchez MA, Vasquez M, Gonzalez B	Appl Environ Microbiol	10.1128/AEM.70.12.7567-7570.2004	2004
Genetics	Localization and characterization of two novel genes encoding stereospecific dioxygenases catalyzing 2(2,4-dichlorophenoxy)propionate cleavage in Delftia acidovorans MC1.	Schleinitz KM, Kleinsteuber S, Vallaeys T, Babel W	Appl Environ Microbiol	10.1128/AEM.70.9.5357-5365.2004	2004
Metabolism	A novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, Rhodococcus opacus SAO101.	Kitagawa W, Kimura N, Kamagata Y	J Bacteriol	10.1128/JB.186.15.4894-4902.2004	2004
Metabolism	Genetic organization of the catabolic plasmid pJP4 from Ralstonia eutropha JMP134 (pJP4) reveals mechanisms of adaptation to chloroaromatic pollutants and evolution of specialized chloroaromatic degradation pathways.	Trefault N, De la Iglesia R, Molina AM, Manzano M, Ledger T, Perez-Pantoja D, Sanchez MA, Stuardo M, Gonzalez B	Environ Microbiol	10.1111/j.1462-2920.2004.00596.x	2004
Metabolism	Mutation analysis of the different tfd genes for degradation of chloroaromatic compounds in Ralstonia eutropha JMP134.	Laemmli C, Werlen C, van der Meer JR	Arch Microbiol	10.1007/s00203-003-0634-4	2003
Enzymology	A monooxygenase catalyzes sequential dechlorinations of 2,4,6-trichlorophenol by oxidative and hydrolytic reactions.	Xun L, Webster CM	J Biol Chem	10.1074/jbc.M312072200	2003
Metabolism	Efficient degradation of 2,4,6-Trichlorophenol requires a set of catabolic genes related to tcp genes from Ralstonia eutropha JMP134(pJP4).	Matus V, Sanchez MA, Martinez M, Gonzalez B	Appl Environ Microbiol	10.1128/AEM.69.12.7108-7115.2003	2003
Metabolism	Bacterial conversion of hydroxylamino aromatic compounds by both lyase and mutase enzymes involves intramolecular transfer of hydroxyl groups.	Nadeau LJ, He Z, Spain JC	Appl Environ Microbiol	10.1128/AEM.69.5.2786-2793.2003	2003
Metabolism	The role of cell bioaugmentation and gene bioaugmentation in the remediation of co-contaminated soils.	Pepper IL, Gentry TJ, Newby DT, Roane TM, Josephson KL	Environ Health Perspect	10.1289/ehp.02110s6943	2002
Metabolism	Efficient turnover of chlorocatechols is essential for growth of Ralstonia eutropha JMP134(pJP4) in 3-chlorobenzoic acid.	Perez-Pantoja D, Ledger T, Pieper DH, Gonzalez B	J Bacteriol	10.1128/JB.185.5.1534-1542.2003	2003
Metabolism	Monitoring gene expression in mixed microbial communities by using DNA microarrays.	Dennis P, Edwards EA, Liss SN, Fulthorpe R	Appl Environ Microbiol	10.1128/AEM.69.2.769-778.2003	2003
Metabolism	Novel insights into the interplay between peripheral reactions encoded by xyl genes and the chlorocatechol pathway encoded by tfd genes for the degradation of chlorobenzoates by Ralstonia eutropha JMP134.	Ledger T, Pieper DH, Perez-Pantoja D, Gonzalez B	Microbiology (Reading)	10.1099/00221287-148-11-3431	2002
Metabolism	Formation of protoanemonin from 2-chloro-cis,cis-muconate by the combined action of muconate cycloisomerase and muconolactone isomerase.	Skiba A, Hecht V, Pieper DH	J Bacteriol	10.1128/JB.184.19.5402-5409.2002	2002
Metabolism	Importance of different tfd genes for degradation of chloroaromatics by Ralstonia eutropha JMP134.	Plumeier I, Perez-Pantoja D, Heim S, Gonzalez B, Pieper DH	J Bacteriol	10.1128/JB.184.15.4054-4064.2002	2002
Metabolism	The copy number of the catabolic plasmid pJP4 affects growth of Ralstonia eutropha JMP134 (pJP4) on 3-chlorobenzoate.	Trefault N, Clement P, Manzano M, Pieper DH, Gonzalez B	FEMS Microbiol Lett	10.1111/j.1574-6968.2002.tb11250.x	2002
Metabolism	TfdD(II), one of the two chloromuconate cycloisomerases of Ralstonia eutropha JMP134 (pJP4), cannot efficiently convert 2-chloro- cis, cis-muconate to trans-dienelactone to allow growth on 3-chlorobenzoate.	Laemmli CM, Schonenberger R, Suter M, Zehnder AJ, van der Meer JR	Arch Microbiol	10.1007/s00203-002-0417-3	2002
Metabolism	Genetic and biochemical characterization of a 2,4,6-trichlorophenol degradation pathway in Ralstonia eutropha JMP134.	Louie TM, Webster CM, Xun L	J Bacteriol	10.1128/JB.184.13.3492-3500.2002	2002
	Expression of tfdA genes in aquatic microbial communities during acclimation to 2,4-dichlorophenoxyacetic acid.	Lipthay JR, Aamand J, Barkay T	FEMS Microbiol Ecol	10.1111/j.1574-6941.2002.tb00953.x	2002
Metabolism	Chemotaxis of Ralstonia eutropha JMP134(pJP4) to the herbicide 2,4-dichlorophenoxyacetate.	Hawkins AC, Harwood CS	Appl Environ Microbiol	10.1128/AEM.68.2.968-972.2002	2002
Metabolism	Novel 2,4-dichlorophenoxyacetic acid degradation genes from oligotrophic Bradyrhizobium sp. strain HW13 isolated from a pristine environment.	Kitagawa W, Takami S, Miyauchi K, Masai E, Kamagata Y, Tiedje JM, Fukuda M	J Bacteriol	10.1128/JB.184.2.509-518.2002	2002
Metabolism	Molecular characterization of a deletion/duplication rearrangement in tfd genes from Ralstonia eutropha JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid.	Clement P, Pieper DH, Gonzalez B	Microbiology (Reading)	10.1099/00221287-147-8-2141	2001
Metabolism	Dual-bioaugmentation strategy to enhance remediation of cocontaminated soil.	Roane TM, Josephson KL, Pepper IL	Appl Environ Microbiol	10.1128/AEM.67.7.3208-3215.2001	2001
Metabolism	The chlorocatechol degradative genes, tfdT-CDEF, of Burkholderia sp. strain NK8 are involved in chlorobenzoate degradation and induced by chlorobenzoates and chlorocatechols.	Liu S, Ogawa N, Miyashita K	Gene	10.1016/s0378-1119(01)00435-8	2001
Metabolism	2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenases from Burkholderia cepacia 2a and Ralstonia eutropha JMP134.	Poh R, Xia X, Bruce IJ, Smith AR	Microbios		2001
Metabolism	A calorimetrically based method to convert toxic compounds into poly-3-hydroxybutyrate and to determine the efficiency and velocity of conversion.	Maskow T, Babel W	Appl Microbiol Biotechnol	10.1007/s002530000546	2001
	A bioluminescent whole-cell reporter for detection of 2, 4-dichlorophenoxyacetic acid and 2,4-dichlorophenol in soil.	Hay AG, Rice JF, Applegate BM, Bright NG, Sayler GS	Appl Environ Microbiol	10.1128/AEM.66.10.4589-4594.2000	2000
Metabolism	Degradation of 2,4,6-trichlorophenol via chlorohydroxyquinol in Ralstonia eutropha JMP134 and JMP222.	Padilla L, Matus V, Zenteno P, Gonzalez B	J Basic Microbiol	10.1002/1521-4028(200008)40:4<243::AID-JOBM243>3.0.CO;2-D	2000
Metabolism	Comparison of 2,4-dichlorophenoxyacetic acid degradation and plasmid transfer in soil resulting from bioaugmentation with two different pJP4 donors.	Newby DT, Gentry TJ, Pepper IL	Appl Environ Microbiol	10.1128/AEM.66.8.3399-3407.2000	2000
Metabolism	Characterization of a second tfd gene cluster for chlorophenol and chlorocatechol metabolism on plasmid pJP4 in Ralstonia eutropha JMP134(pJP4).	Laemmli CM, Leveau JH, Zehnder AJ, van der Meer JR	J Bacteriol	10.1128/JB.182.15.4165-4172.2000	2000
	Deletions of mob and tra pJP4 transfer functions after mating of Ralstonia eutropha JMP134 (pJP4) with Escherichia coli harboring F'::Tn10.	Clement P, Springael D, Gonzalez B	Can J Microbiol		2000
Metabolism	Role of tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II) gene modules in catabolism of 3-chlorobenzoate by Ralstonia eutropha JMP134(pJP4).	Perez-Pantoja D, Guzman L, Manzano M, Pieper DH, Gonzalez B	Appl Environ Microbiol	10.1128/AEM.66.4.1602-1608.2000	2000
Metabolism	Detection and characterization of plasmid pJP4 transfer to indigenous soil bacteria.	Newby DT, Josephson KL, Pepper IL	Appl Environ Microbiol	10.1128/AEM.66.1.290-296.2000	2000
Genetics	Adaptive responses of Ralstonia eutropha to feast and famine conditions analysed by flow cytometry.	Muller S, Bley T, Babel W	J Biotechnol	10.1016/s0168-1656(99)00130-3	1999
Metabolism	X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading alpha-ketoglutarate dioxygenase.	Cosper NJ, Stalhandske CM, Saari RE, Hausinger RP, Scott RA	J Biol Inorg Chem	10.1007/s007750050295	1999
Metabolism	Dynamics of multigene expression during catabolic adaptation of Ralstonia eutropha JMP134 (pJP4) to the herbicide 2, 4-dichlorophenoxyacetate.	Leveau JH, Konig F, Fuchslin H, Werlen C, Van Der Meer JR	Mol Microbiol	10.1046/j.1365-2958.1999.01483.x	1999
Enzymology	Substrate specificities of the chloromuconate cycloisomerases from Pseudomonas sp. B13, Ralstonia eutropha JMP134 and Pseudomonas sp. P51.	Vollmer MD, Schell U, Seibert V, Lakner S, Schlomann M	Appl Microbiol Biotechnol	10.1007/s002530051438	1999
Metabolism	Chemoselective nitro group reduction and reductive dechlorination initiate degradation of 2-chloro-5-nitrophenol by Ralstonia eutropha JMP134.	Schenzle A, Lenke H, Spain JC, Knackmuss HJ	Appl Environ Microbiol	10.1128/AEM.65.6.2317-2323.1999	1999
Enzymology	3-Hydroxylaminophenol mutase from Ralstonia eutropha JMP134 catalyzes a Bamberger rearrangement.	Schenzle A, Lenke H, Spain JC, Knackmuss HJ	J Bacteriol	10.1128/JB.181.5.1444-1450.1999	1999
Phenotype	Monoclonal antibodies to 2,4-dichlorophenol hydroxylase as probes for the 2,4-D-degradative phenotype.	Farhana L, Fulthorpe RR, Harbour C, New PB	Can J Microbiol	10.1139/cjm-44-10-920	1998
Enzymology	Whole-cell kinetics of trichloroethylene degradation by phenol hydroxylase in a ralstonia eutropha JMP134 derivative	Ayoubi PJ, Harker AR	Appl Environ Microbiol	10.1128/AEM.64.11.4353-4356.1998	1998
Metabolism	Amplification of putative chlorocatechol dioxygenase gene fragments from alpha- and beta-Proteobacteria.	Leander M, Vallaeys T, Fulthorpe R	Can J Microbiol	10.1139/cjm-44-5-482	1998
Metabolism	The tfdK gene product facilitates uptake of 2,4-dichlorophenoxyacetate by Ralstonia eutropha JMP134(pJP4).	Leveau JH, Zehnder AJ, van der Meer JR	J Bacteriol	10.1128/JB.180.8.2237-2243.1998	1998
Metabolism	Ascorbic acid-dependent turnover and reactivation of 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase using thiophenoxyacetic acid.	Saari RE, Hausinger RP	Biochemistry	10.1021/bi972388p	1998
Metabolism	Characterization of a gene cluster from Ralstonia eutropha JMP134 encoding metabolism of 4-methylmuconolactone.	Erb RW, Timmis KN, Pieper DH	Gene	10.1016/s0378-1119(97)00565-9	1998
Genetics	Genetic characterization of insertion sequence ISJP4 on plasmid pJP4 from Ralstonia eutropha JMP134.	Leveau JH, van der Meer JR	Gene	10.1016/s0378-1119(97)00460-5	1997
Enzymology	Cloning and characterization of the regulatory genes phlR1 and phlR2 involved in phenol metabolism from Alcaligenes eutrophus JMP134.	Kim Y, Harker AR	Mol Cells		1997
Metabolism	Involvement of two alpha-ketoglutarate-dependent dioxygenases in enantioselective degradation of (R)- and (S)-mecoprop by Sphingomonas herbicidovorans MH.	Nickel K, Suter MJ, Kohler HP	J Bacteriol	10.1128/jb.179.21.6674-6679.1997	1997
Enzymology	Evidence for an isomeric muconolactone isomerase involved in the metabolism of 4-methylmuconolactone by Alcaligenes eutrophus JMP134.	Prucha M, Peterseim A, Pieper DH	Arch Microbiol	10.1007/s002030050466	1997
Metabolism	Pristine environments harbor a new group of oligotrophic 2,4-dichlorophenoxyacetic acid-degrading bacteria.	Kamagata Y, Fulthorpe RR, Tamura K, Takami H, Forney LJ, Tiedje JM	Appl Environ Microbiol	10.1128/aem.63.6.2266-2272.1997	1997
Metabolism	Biodegradation of 2,4-Dichlorophenol through a Distal meta-Fission Pathway.	Koh S, McCullar MV, Focht DD	Appl Environ Microbiol	10.1128/aem.63.5.2054-2057.1997	1997
	Genetic Diversity through the Looking Glass: Effect of Enrichment Bias.	Dunbar J, White S, Forney L	Appl Environ Microbiol	10.1128/aem.63.4.1326-1331.1997	1997
Enzymology	The 2,4-dichlorophenol hydroxylase of Alcaligenes eutrophus JMP134 is a homotetramer.	Farhana L, New PB	Can J Microbiol	10.1139/m97-027	1997
	Identification of the Inducing Agent of the 2,4-Dichlorophenoxyacetic Acid Pathway Encoded by Plasmid pJP4.	Filer K, Harker AR	Appl Environ Microbiol	10.1128/aem.63.1.317-320.1997	1997
Metabolism	Degradation of Chlorophenols by Alcaligenes eutrophus JMP134(pJP4) in Bleached Kraft Mill Effluent.	Valenzuela J, Bumann U, Cespedes R, Padilla L, Gonzalez B	Appl Environ Microbiol	10.1128/aem.63.1.227-232.1997	1997
Metabolism	The tfdR gene product can successfully take over the role of the insertion element-inactivated TfdT protein as a transcriptional activator of the tfdCDEF gene cluster, which encodes chlorocatechol degradation in Ralstonia eutropha JMP134(pJP4)	Leveau JH, van der Meer JR	J Bacteriol	10.1128/jb.178.23.6824-6832.1996	1996
Metabolism	Constitutive expression of the cloned phenol hydroxylase gene(s) from Alcaligenes eutrophus JMP134 and concomitant trichloroethylene oxidation.	Kim Y, Ayoubi P, Harker AR	Appl Environ Microbiol	10.1128/aem.62.9.3227-3233.1996	1996
Metabolism	Gene transfer of Alcaligenes eutrophus JMP134 plasmid pJP4 to indigenous soil recipients.	DiGiovanni GD, Neilson JW, Pepper IL, Sinclair NA	Appl Environ Microbiol	10.1128/aem.62.7.2521-2526.1996	1996
Enzymology	Capture of a catabolic plasmid that encodes only 2,4-dichlorophenoxyacetic acid:alpha-ketoglutaric acid dioxygenase (TfdA) by genetic complementation.	Top EM, Maltseva OV, Forney LJ	Appl Environ Microbiol	10.1128/aem.62.7.2470-2476.1996	1996
Enzymology	Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC.	Suwa Y, Wright AD, Fukimori F, Nummy KA, Hausinger RP, Holben WE, Forney LJ	Appl Environ Microbiol	10.1128/aem.62.7.2464-2469.1996	1996
Cultivation	Growth and viability of Alcaligenes eutrophus JMP134 in seawater as affected by substrate and nutrient amendment.	Nybroe O, Einarson K, Ahl T	Lett Appl Microbiol	10.1111/j.1472-765x.1996.tb01180.x	1996
Enzymology	Maleylacetate reductases in chloroaromatic-degrading bacteria using the modified ortho pathway: comparison of catalytic properties.	Muller D, Schlomann M, Reineke W	J Bacteriol	10.1128/jb.178.1.298-300.1996	1996
Enzymology	Substrate diversity and expression of the 2,4,5-trichlorophenoxyacetic acid oxygenase from Burkholderia cepacia AC1100.	Danganan CE, Shankar S, Ye RW, Chakrabarty AM	Appl Environ Microbiol	10.1128/aem.61.12.4500-4504.1995	1995
Metabolism	Extraction and purification of microbial DNA from petroleum-contaminated soils and detection of low numbers of toluene, octane and pesticide degraders by multiplex polymerase chain reaction and Southern analysis.	Knaebel DB, Crawford RL	Mol Ecol	10.1111/j.1365-294x.1995.tb00258.x	1995
Metabolism	2,4-Dichlorophenoxyacetic acid-degrading bacteria contain mosaics of catabolic genes.	Fulthorpe RR, McGowan C, Maltseva OV, Holben WE, Tiedje JM	Appl Environ Microbiol	10.1128/aem.61.9.3274-3281.1995	1995
Metabolism	Evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway.	Kasberg T, Daubaras DL, Chakrabarty AM, Kinzelt D, Reineke W	J Bacteriol	10.1128/jb.177.13.3885-3889.1995	1995
Metabolism	Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism.	Hausinger RP, Fukumori F	Environ Health Perspect	10.1289/ehp.95103s437	1995
Metabolism	Characterization of diverse 2,4-dichlorophenoxyacetic acid-degradative plasmids isolated from soil by complementation.	Top EM, Holben WE, Forney LJ	Appl Environ Microbiol	10.1128/aem.61.5.1691-1698.1995	1995
Metabolism	Genetic and molecular analysis of a regulatory region of the herbicide 2,4-dichlorophenoxyacetate catabolic plasmid pJP4.	You IS, Ghosal D	Mol Microbiol	10.1111/j.1365-2958.1995.tb02304.x	1995
Metabolism	Degradation of trichlorophenols by Alcaligenes eutrophus JMP134.	Clement P, Matus V, Cardenas L, Gonzalez B	FEMS Microbiol Lett	10.1111/j.1574-6968.1995.tb07449.x	1995
Enzymology	Chlorocatechol 1,2-dioxygenase from Rhodococcus erythropolis 1CP. Kinetic and immunochemical comparison with analogous enzymes from gram-negative strains.	Maltseva OV, Solyanikova IP, Golovleva LA	Eur J Biochem	10.1111/j.1432-1033.1994.01053.x	1994
Cultivation	Frequency of horizontal gene transfer of a large catabolic plasmid (pJP4) in soil.	Neilson JW, Josephson KL, Pepper IL, Arnold RB, Di Giovanni GD, Sinclair NA	Appl Environ Microbiol	10.1128/aem.60.11.4053-4058.1994	1994
Enzymology	Inability of muconate cycloisomerases to cause dehalogenation during conversion of 2-chloro-cis,cis-muconate.	Vollmer MD, Fischer P, Knackmuss HJ, Schlomann M	J Bacteriol	10.1128/jb.176.14.4366-4375.1994	1994
Genetics	Analysis of duplicated gene sequences associated with tfdR and tfdS in Alcaligenes eutrophus JMP134.	Matrubutham U, Harker AR	J Bacteriol	10.1128/jb.176.8.2348-2353.1994	1994
	Crystal structure of chloromuconate cycloisomerase from Alcaligenes eutrophus JMP134 (pJP4) at 3 A resolution.	Hoier H, Schlomann M, Hammer A, Glusker JP, Carrell HL, Goldman A, Stezowski JJ, Heinemann U	Acta Crystallogr D Biol Crystallogr	10.1107/S090744499300900X	1994
Enzymology	Purification and characterization of maleylacetate reductase from Alcaligenes eutrophus JMP134(pJP4).	Seibert V, Stadler-Fritzsche K, Schlomann M	J Bacteriol	10.1128/jb.175.21.6745-6754.1993	1993
Enzymology	Crystallization and preliminary X-ray data of chloromuconate cycloisomerase from Alcaligenes eutrophus JMP134 (pJP4).	Hammer A, Hildenbrand T, Hoier H, Ngai KL, Schlomann M, Stezowski JJ	J Mol Biol	10.1006/jmbi.1993.1385	1993
Metabolism	Alcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase.	Fukumori F, Hausinger RP	J Bacteriol	10.1128/jb.175.7.2083-2086.1993	1993
	Preservation of immobilized bacterial cell-matrix by drying for direct use in microbial sensors.	Malik KA, Beyersdorf-Radeck B, Schmid RD	World J Microbiol Biotechnol	10.1007/BF00327847	1993
Metabolism	Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134.	Vollmer MD, Stadler-Fritzsche K, Schlomann M	Arch Microbiol	10.1007/BF00250280	1993
Metabolism	Polymerase chain reaction and gene probe detection of the 2,4-dichlorophenoxyacetic acid degradation plasmid, pJP4.	Neilson JW, Josephson KL, Pillai SD, Pepper IL	Appl Environ Microbiol	10.1128/aem.58.4.1271-1275.1992	1992
Metabolism	Sequence analysis of the Pseudomonas sp. strain P51 tcb gene cluster, which encodes metabolism of chlorinated catechols: evidence for specialization of catechol 1,2-dioxygenases for chlorinated substrates.	van der Meer JR, Eggen RI, Zehnder AJ, de Vos WM	J Bacteriol	10.1128/jb.173.8.2425-2434.1991	1991
Metabolism	Mineralization of 2,4-dichlorophenoxyacetic acid (2,4-D) in soil inoculated with Pseudomonas cepacia DBO1(pRO101), Alcaligenes eutrophus AEO106(pRO101) and Alcaligenes eutrophus JMP134(pJP4): effects of inoculation level and substrate concentration.	Jacobsen CS, Pedersen JC	Biodegradation	10.1007/BF00114557	1991
Metabolism	Enzymatic formation, stability, and spontaneous reactions of 4-fluoromuconolactone, a metabolite of the bacterial degradation of 4-fluorobenzoate.	Schlomann M, Fischer P, Schmidt E, Knackmuss HJ	J Bacteriol	10.1128/jb.172.9.5119-5129.1990	1990
Enzymology	Different types of dienelactone hydrolase in 4-fluorobenzoate-utilizing bacteria.	Schlomann M, Schmidt E, Knackmuss HJ	J Bacteriol	10.1128/jb.172.9.5112-5118.1990	1990
Metabolism	Degradation of the chlorinated phenoxyacetate herbicides 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid by pure and mixed bacterial cultures.	Haugland RA, Schlemm DJ, Lyons RP 3rd, Sferra PR, Chakrabarty AM	Appl Environ Microbiol	10.1128/aem.56.5.1357-1362.1990	1990
Enzymology	Organization and sequence analysis of the 2,4-dichlorophenol hydroxylase and dichlorocatechol oxidative operons of plasmid pJP4.	Perkins EJ, Gordon MP, Caceres O, Lurquin PF	J Bacteriol	10.1128/jb.172.5.2351-2359.1990	1990
Metabolism	Trichloroethylene degradation by two independent aromatic-degrading pathways in Alcaligenes eutrophus JMP134.	Harker AR, Kim Y	Appl Environ Microbiol	10.1128/aem.56.4.1179-1181.1990	1990
Enzymology	Operon structure and nucleotide homology of the chlorocatechol oxidation genes of plasmids pJP4 and pAC27.	Ghosal D, You IS	Gene	10.1016/0378-1119(89)90108-x	1989
Metabolism	Phenoxyacetic acid degradation by the 2,4-dichlorophenoxyacetic acid (TFD) pathway of plasmid pJP4: mapping and characterization of the TFD regulatory gene, tfdR.	Harker AR, Olsen RH, Seidler RJ	J Bacteriol	10.1128/jb.171.1.314-320.1989	1989
Pathogenicity	Transfer and expression of lithoautotrophy and denitrification in a host lacking these metabolic activities.	Schneider B, Nies A, Friedrich B	Appl Environ Microbiol	10.1128/aem.54.12.3173-3176.1988	1988
Enzymology	Duplication of a 2,4-dichlorophenoxyacetic acid monooxygenase gene in Alcaligenes eutrophus JMP134(pJP4).	Perkins EJ, Lurquin PF	J Bacteriol	10.1128/jb.170.12.5669-5672.1988	1988
Enzymology	Nucleotide homology and organization of chlorocatechol oxidation genes of plasmids pJP4 and pAC27.	Ghosal D, You IS	Mol Gen Genet	10.1007/BF00338401	1988
Enzymology	Analysis, cloning, and high-level expression of 2,4-dichlorophenoxyacetate monooxygenase gene tfdA of Alcaligenes eutrophus JMP134.	Streber WR, Timmis KN, Zenk MH	J Bacteriol	10.1128/jb.169.7.2950-2955.1987	1987
Metabolism	Transposon mutagenesis and cloning analysis of the pathways for degradation of 2,4-dichlorophenoxyacetic acid and 3-chlorobenzoate in Alcaligenes eutrophus JMP134(pJP4).	Don RH, Weightman AJ, Knackmuss HJ, Timmis KN	J Bacteriol	10.1128/jb.161.1.85-90.1985	1985
Enzymology	The identification and cloning of genes encoding haloaromatic catabolic enzymes and the construction of hybrid pathways for substrate mineralization.	Weightman AJ, Don RH, Lehrbach PR, Timmis KN	Basic Life Sci	10.1007/978-1-4684-4715-6_4	1984

References

#1581	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4058
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Cupriavidus necator (DSM 4058, JMP134, NCIB 12592)

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