Cupriavidus necator (DSM 428, ATCC 17699, KCTC 22496)

Name: Cupriavidus necator (DSM 428, ATCC 17699, KCTC 22496)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2008

Type strain

Strain Designation pHG1, H 16, H16, Wilde H16, strain S-10-1, Schlegel H16

Culture col. no. DSM 428 ATCC 17699 KCTC 22496 NCIB 10442 pHG1 19 more

NCBI tax ID(s) 381666 106590

Special Collections DSMZ JCM KCTC JMRC

History DSMZ <- IMG (Alcaligenes eutrophus) <- E. Wilde, H 16 JCM <-- IAM 12567 <-- NCIB 10442 <-- H. G. Schlegel H16.

Links

Cupriavidus necator pHG1 is a bacterium that was isolated from sludge.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 13563

LMG 1201,ATCC 17699,ATCC 23440,CCUG 13797,DSM 428,NCIB 10442,NCIMB 10442,IAM12567,CCM 3726,CECT 4635,CCRC 13036,KCTC 1006,KCTC 2660,NCCB 82042,LMD 82.42,BCRC 13036,CCT 2489,CCUG 52967,JCM 20644

@ref 20215

Last LPSN update 2026-05-29 08:25:23

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Cupriavidus

Species Cupriavidus necator

Full scientific name Cupriavidus necator Makkar and Casida 1987

BacDive ID	Other strains from **Cupriavidus necator** (39)	Type strain
2033	C. necator N-1, Jr N-1, DSM 13513, ATCC 43291, CCUG ... (type strain)
330	C. necator 1978, DSM 2625, CCUG 52964, ATCC 33178, LMG ...
1998	C. necator H 1, CCUG 52966, LMG 1200, ATCC 23442, DSM ...
1999	C. necator H 20, DSM 530, ATCC 17700
2001	C. necator H 16 G+1, H 16 G⁺1, DSM 538
2002	C. necator H 16 F 34, DSM 539
2003	C. necator H 16 F 29, DSM 540
2004	C. necator H 16 PHB-4, H 16 PHB^-4, DSM 541
2005	C. necator LH-7, LH^-7, DSM 416
2006	C. necator PH-9, PH^-9, DSM 418
2007	C. necator PH-4, PH^-4, DSM 422
2009	C. necator H16 25/1, DSM 430
2010	C. necator B 19, DSM 515, JCM 20894, CCUG 13796, IAM ...
2011	C. necator G 27, DSM 516
2012	C. necator G 29, DSM 517
2013	C. necator DSM 518
2014	C. necator H 16 G+7, H 16 G⁺7, DSM 542
2015	C. necator G 29 G+1, G 29 G⁺1, DSM 543
2016	C. necator H 20 G+3, H 20 G⁺3, DSM 544
2017	C. necator H 1 G+3, H 1 G⁺3, DSM 545
2018	C. necator H 1 G+4, H 1 G⁺4, DSM 546
2019	C. necator H 20 G+1, H 20 G⁺1, DSM 547
2020	C. necator H 16 G+3, H 16 G⁺3, DSM 551
2021	C. necator H16 pHS4, DSM 3102
2022	C. necator JMP363 Smr, pJP2, DSM 4056
2023	C. necator JMP364, pJP3, JMP364 Smr, DSM 4057
2024	C. necator JMP134, pJP4, DSM 4058, NCIB 12592
2025	C. necator JMP365, pJP5, JMP365 Smr, DSM 4059
2026	C. necator JMP366, pJP7, JMP366 Smr, DSM 4060
2027	C. necator TA06, DSM 4182
2028	C. necator H850, DSM 5536, NRRL B-15940
2031	C. necator Bo, Tfa 17, DSM 11098
2032	C. necator H850-4LT, DSM 13439
2034	C. necator C145, DSM 15443
2035	C. necator HF39, DSM 15444
2036	C. necator DSM 30029, ATCC 25207, NRRL B-2804, CCUG ...
162984	C. necator JCM 20695, IAM 12628
163005	C. necator JCM 20908, ATCC 17702, IAM 13548, LMG 1203
163006	C. necator JCM 20909, ATCC 17708, IAM 13549, LMG 1208

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	96.249
125438	negative	97.5

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
197	MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81)		Medium recipe at MediaDive	Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water
197	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
197	positive	growth	30
67770	positive	growth	25

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.234

Spore formation

@ref	Spore formation	Confidence
125439		99.221

Compound production

@ref	Compound
197	PHB
197	hydrogenase
197	phenylalanine, hydroxylation of
197	single cell protein

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	biotin biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	palmitate biosynthesis	100	22 of 22
66794	photosynthesis	100	14 of 14
66794	3-chlorocatechol degradation	100	5 of 5
66794	glycogen metabolism	100	5 of 5
66794	acetate fermentation	100	4 of 4
66794	ethanol fermentation	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	gallate degradation	100	5 of 5
66794	adipate degradation	100	2 of 2
66794	aspartate and asparagine metabolism	100	9 of 9
66794	hydrogen production	100	5 of 5
66794	lipoate biosynthesis	100	5 of 5
66794	quinate degradation	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	resorcinol degradation	100	2 of 2
66794	butanoate fermentation	100	4 of 4
66794	L-lactaldehyde degradation	100	3 of 3
66794	cardiolipin biosynthesis	100	7 of 7
66794	Entner Doudoroff pathway	100	10 of 10
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	denitrification	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	taurine degradation	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	CO2 fixation in Crenarchaeota	100	9 of 9
66794	sulfopterin metabolism	100	4 of 4
66794	ubiquinone biosynthesis	100	7 of 7
66794	ppGpp biosynthesis	100	4 of 4
66794	allantoin degradation	100	9 of 9
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	ribulose monophosphate pathway	100	2 of 2
66794	cyanate degradation	100	3 of 3
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	3-phenylpropionate degradation	93.33	14 of 15
66794	citric acid cycle	92.86	13 of 14
66794	glutathione metabolism	92.86	13 of 14
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	sulfate reduction	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	phenol degradation	90	18 of 20
66794	starch degradation	90	9 of 10
66794	propionate fermentation	90	9 of 10
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	d-mannose degradation	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	gluconeogenesis	87.5	7 of 8
66794	alanine metabolism	86.21	25 of 29
66794	vitamin B1 metabolism	84.62	11 of 13
66794	glycolate and glyoxylate degradation	83.33	5 of 6
66794	purine metabolism	82.98	78 of 94
66794	histidine metabolism	82.76	24 of 29
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	proline metabolism	81.82	9 of 11
66794	vitamin B6 metabolism	81.82	9 of 11
66794	tryptophan metabolism	81.58	31 of 38
66794	vitamin K metabolism	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	heme metabolism	78.57	11 of 14
66794	lysine metabolism	78.57	33 of 42
66794	tyrosine metabolism	78.57	11 of 14
66794	non-pathway related	76.32	29 of 38
66794	pyrimidine metabolism	75.56	34 of 45
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	degradation of aromatic, nitrogen containing compounds	75	9 of 12
66794	CMP-KDO biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	ketogluconate metabolism	75	6 of 8
66794	lactate fermentation	75	3 of 4
66794	oxidative phosphorylation	74.73	68 of 91
66794	flavin biosynthesis	73.33	11 of 15
66794	methionine metabolism	73.08	19 of 26
66794	cysteine metabolism	72.22	13 of 18
66794	propanol degradation	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	urea cycle	69.23	9 of 13
66794	androgen and estrogen metabolism	68.75	11 of 16
66794	degradation of sugar acids	68	17 of 25
66794	lipid metabolism	67.74	21 of 31
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	nitrate assimilation	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	metabolism of disaccharids	63.64	7 of 11
66794	degradation of sugar alcohols	62.5	10 of 16
66794	arginine metabolism	62.5	15 of 24
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	creatinine degradation	60	3 of 5
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	degradation of pentoses	57.14	16 of 28
66794	benzoyl-CoA degradation	57.14	4 of 7
66794	glycine metabolism	50	5 of 10
66794	selenocysteine biosynthesis	50	3 of 6
66794	toluene degradation	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	cyclohexanol degradation	50	2 of 4
66794	phenylmercury acetate degradation	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	coenzyme M biosynthesis	50	5 of 10
66794	polyamine pathway	47.83	11 of 23
66794	vitamin B12 metabolism	47.06	16 of 34
66794	cholesterol biosynthesis	45.45	5 of 11
66794	degradation of hexoses	44.44	8 of 18
66794	bile acid biosynthesis, neutral pathway	41.18	7 of 17
66794	carotenoid biosynthesis	40.91	9 of 22
66794	glycine betaine biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	factor 420 biosynthesis	40	2 of 5
66794	arachidonic acid metabolism	38.89	7 of 18
66794	carnitine metabolism	37.5	3 of 8
66794	ascorbate metabolism	36.36	8 of 22
66794	enterobactin biosynthesis	33.33	1 of 3
66794	methane metabolism	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	methanogenesis from CO2	33.33	4 of 12
66794	sphingosine metabolism	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	phosphatidylethanolamine bioynthesis	30.77	4 of 13
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	chlorophyll metabolism	27.78	5 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	alginate biosynthesis	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
197	sludge	Göttingen, Weende-Quelle	Germany	DEU	Europe
67770	Sludge	Göttingen	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
197	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM479872v1 assembly for Cupriavidus necator H16	complete	GCA_004798725	381666.45	2858669534	381666	93.57
66792	ASM928v2 assembly for Cupriavidus necator H16	complete	GCA_000009285	381666.6	640427136	381666	92.95

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Ralstonia eutropha DNA for 16S ribosomal RNA	D88002	1468		106590

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM479872v1 assembly for Cupriavidus necator H16
BV-BRC: 381666.45

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.23	no
125439	gram_stain	BacteriaNetⓘ	negative	96.25	no
125439	motility	BacteriaNetⓘ	yes	68.32	no
125439	spore_formation	BacteriaNetⓘ	no	99.22	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Cupriavidus necator H16 H16
NCBI: GCA_004798725.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.50	no
125438	anaerobic	anaerobicⓘ	no	94.71	no
125438	aerobic	aerobicⓘ	yes	82.23	no
125438	spore-forming	spore-formingⓘ	no	88.79	no
125438	thermophilic	thermophileⓘ	no	98.49	no
125438	flagellated	motile2+ⓘ	yes	83.92	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Bacterial species-structure-property relationships of polyhydroxyalkanoate biopolymers produced on simple sugars for thin film applications.	Attenborough E, Yazdan Parast F, Nosrati R, Banaszak Holl MM, van 't Hag L.	Microb Cell Fact	10.1186/s12934-025-02833-7	2025
Cultivation	Glycerol-supplemented medium promotes transition of Cupriavidus necator from heterotrophic to lithoautotrophic growth.	Castro Gonzalez I, Gorret N, Lauterbach L, Guillouet SE.	Biotechnol Lett	10.1007/s10529-025-03671-8	2025
	Cultivation of Cupriavidus necatorstrains on hydrolyzed lignocellulosic feedstocks widely available in Europe.	Alhafiz HA, Longus K, Verlinden RAJ, Lambauer V, Kruschitz A, Kratzer R.	Biotechnol Rep (Amst)	10.1016/j.btre.2025.e00899	2025
Transcriptome	A promoter library for tuning gene expression in Cupriavidus necator under autotrophic conditions.	Kitagawa W, Igarashi K, Nagasawa R, Kakizawa S, Horino M, Fujishima K, Fukui T, Kato S.	Front Bioeng Biotechnol	10.3389/fbioe.2025.1595440	2025
	Efficient Production of High-Concentration Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO₂ Employing the Recombinant of Cupriavidus necator.	Tanaka K, Orita I, Fukui T.	Bioengineering (Basel)	10.3390/bioengineering12060557	2025
	Recent Advances in Food Waste Transformations into Essential Bioplastic Materials.	Giwa AS, Shafique E, Ali N, Vakili M.	Molecules	10.3390/molecules29163838	2024
	Production of succinate with two CO₂ fixation reactions from fatty acids in Cupriavidus necator H16.	Li L, Zhou X, Gao Z, Xiong P, Liu X.	Microb Cell Fact	10.1186/s12934-024-02470-6	2024
Enzymology	Using Cupriavidus necator H16 to Provide a Roadmap for Increasing Electroporation Efficiency in Nonmodel Bacteria.	Vajente M, Clerici R, Ballerstedt H, Blank LM, Schmidt S.	ACS Synth Biol	10.1021/acssynbio.4c00380	2025
	CnRed: Efficient, Marker-free Genome Engineering of Cupriavidus necator H16 by Adapted Lambda Red Recombineering.	Arhar S, Pirchner J, Stolterfoht-Stock H, Reicher K, Kourist R, Emmerstorfer-Augustin A.	ACS Synth Biol	10.1021/acssynbio.4c00757	2025
	Bio-electrosynthesis of polyhydroxybutyrate and surfactants in microbial fuel cells: a preliminary study.	Nastro RA, Kuppam C, Toscanesi M, Trifuoggi M, Pietrelli A, Pasquale V, Avignone-Rossa C.	Front Microbiol	10.3389/fmicb.2025.1372302	2025
	Synthesis and Properties of Degradable Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] Derived from Waste Fish Oil	Volova T, Kiselev E, Sukovatyi A, Zhila N, Sapozhnikova K, Ipatova N, Shishatskii P.	Polymers (Basel)		2025
	Waste to wealth: Polyhydroxyalkanoates (PHA) production from food waste for a sustainable packaging paradigm.	Kusuma HS, Sabita A, Putri NA, Azliza N, Illiyanasafa N, Darmokoesoemo H, Amenaghawon AN, Kurniawan TA.	Food Chem (Oxf)	10.1016/j.fochms.2024.100225	2024
Metabolism	The energy metabolism of Cupriavidus necator in different trophic conditions.	Jahn M, Crang N, Gynna AH, Kabova D, Frielingsdorf S, Lenz O, Charpentier E, Hudson EP.	Appl Environ Microbiol	10.1128/aem.00748-24	2024
	Optimizing Hexose Utilization Pathways of Cupriavidus necator for Improving Growth and L-Alanine Production under Heterotrophic and Autotrophic Conditions.	Wang L, Luo H, Yao B, Yao J, Zhang J.	Int J Mol Sci	10.3390/ijms25010548	2023
Genetics	Whole Genome Sequence Analysis of Cupriavidus necator C39, a Multiple Heavy Metal(loid) and Antibiotic Resistant Bacterium Isolated from a Gold/Copper Mine.	Xie Z, Wang D, Ben Fekih I, Yu Y, Li Y, Alwathnani H, Herzberg M, Rensing C.	Microorganisms	10.3390/microorganisms11061518	2023
	De Novo Synthesis of Poly(3-hydroxybutyrate-co-3-hydroxypropionate) from Oil by Engineered Cupriavidus necator.	Li M, Li W, Zhang T, Guo K, Feng D, Liang F, Xu C, Xian M, Zou H.	Bioengineering (Basel)	10.3390/bioengineering10040446	2023
	Production of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO2 via pH-Stat Jar Cultivation of an Engineered Hydrogen-Oxidizing Bacterium Cupriavidus necator	Tanaka K, Orita I, Fukui T.	Bioengineering (Basel)		2023
	Pseudomonadal itaconate degradation gene cluster encodes enzymes for methylsuccinate utilization.	Gonner L, Cassens EA, Konig S, Berg IA.	Commun Biol	10.1038/s42003-025-08538-2	2025
	CO₂-based production of phytase from highly stable expression plasmids in Cupriavidus necator H16.	Arhar S, Rauter T, Stolterfoht-Stock H, Lambauer V, Kratzer R, Winkler M, Karava M, Kourist R, Emmerstorfer-Augustin A.	Microb Cell Fact	10.1186/s12934-023-02280-2	2024
	An Overview of Biorefinery Waste for Microbial Production of Green Plastic in a Circular Economy^§.	Gudapati G, Veluru S, Bora T, Tukaram Bai M, Dwarapureddy AKP, Reddi GP, Hamzah HT.	Food Technol Biotechnol	10.17113/ftb.63.02.25.8966	2025
	Medium-Chain-Length Fatty Acid Catabolism in Cupriavidus necator H16: Transcriptome Sequencing Reveals Differences from Long-Chain-Length Fatty Acid beta-Oxidation and Involvement of Several Homologous Genes.	Strittmatter CS, Poehlein A, Himmelbach A, Daniel R, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.01428-22	2023
	Production and Properties of Microbial Polyhydroxyalkanoates Synthesized from Hydrolysates of Jerusalem Artichoke Tubers and Vegetative Biomass.	Volova TG, Kiselev EG, Demidenko AV, Zhila NO, Nemtsev IV, Lukyanenko AV.	Polymers (Basel)	10.3390/polym14010132	2021
	Biosynthesis and Properties of Sulfur-Containing Polyhydroxyalkanoates (PHAs) Produced by Wild-Type Strain Cupriavidus necator B-10646.	Zhila NO, Sapozhnikova KY, Berezovskaya AV, Kiselev EG, Shishatskaya EI, Vasiliev AD, Thomas S, Volova TG.	Polymers (Basel)	10.3390/polym15041005	2023
	Engineering osmolysis susceptibility in Cupriavidus necator and Escherichia coli for recovery of intracellular products.	Adams JD, Sander KB, Criddle CS, Arkin AP, Clark DS.	Microb Cell Fact	10.1186/s12934-023-02064-8	2023
	A biopiezocatalyst harnessing mechanical energy to enhance bioplastic production from CO2 and organic carbon	Tremblay P, Xu M, Joya M, Wang Y, He C, Li Z, Li L, Xu K, Feng Y, Zhang T.	Nat Commun		2025
Enzymology	Seamless and orthogonal expression of genetic parts in polyhydroxyalkanoate (PHA)-producing bacterial chassis for plastic bio-upcycling applications.	Minggu MM, Naseron NAH, Shaberi HSA, Muhammad NAN, Baharum SN, Ramzi AB.	MethodsX	10.1016/j.mex.2023.102434	2023
Enzymology	Heterologous constitutive production of short-chain-length polyhydroxyalkanoates in Pseudomonas putida KT2440: the involvement of IbpA inclusion body protein.	Manoli MT, Blanco FG, Rivero-Buceta V, Kniewel R, Alarcon SH, Salgado S, Prieto MA.	Front Bioeng Biotechnol	10.3389/fbioe.2023.1275036	2023
	Recent updates to microbial production and recovery of polyhydroxyalkanoates.	de Melo RN, de Souza Hassemer G, Steffens J, Junges A, Valduga E.	3 Biotech	10.1007/s13205-023-03633-9	2023
	A Review on Enhancing Cupriavidus necator Fermentation for Poly(3-hydroxybutyrate) (PHB) Production From Low-Cost Carbon Sources.	Zhang L, Jiang Z, Tsui TH, Loh KC, Dai Y, Tong YW.	Front Bioeng Biotechnol	10.3389/fbioe.2022.946085	2022
	The use of LipidGreen2 for visualization and quantification of intracellular Poly(3-hydroxybutyrate) in Cupriavidus necator.	Kettner A, Griehl C.	Biochem Biophys Rep	10.1016/j.bbrep.2020.100819	2020
	Biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from CO₂ by a Recombinant Cupriavidusnecator.	Tanaka K, Yoshida K, Orita I, Fukui T.	Bioengineering (Basel)	10.3390/bioengineering8110179	2021
	Fast, inexpensive, and reliable HPLC method to determine monomer fractions in poly(3-hydroxybutyrate-co-3-hydroxyvalerate).	Duvigneau S, Kettner A, Carius L, Griehl C, Findeisen R, Kienle A.	Appl Microbiol Biotechnol	10.1007/s00253-021-11265-3	2021
	Lab-Scale Cultivation of Cupriavidus necator on Explosive Gas Mixtures: Carbon Dioxide Fixation into Polyhydroxybutyrate.	Lambauer V, Kratzer R.	Bioengineering (Basel)	10.3390/bioengineering9050204	2022
Biotechnology	Manipulating Microbial Cell Morphology for the Sustainable Production of Biopolymers.	Kalia VC, Patel SKS, Karthikeyan KK, Jeya M, Kim IW, Lee JK.	Polymers (Basel)	10.3390/polym16030410	2024
Metabolism	Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator.	Jahn M, Crang N, Janasch M, Hober A, Forsstrom B, Kimler K, Mattausch A, Chen Q, Asplund-Samuelsson J, Hudson EP.	Elife	10.7554/elife.69019	2021
Genetics	Complete genome sequence of Photobacterium ganghwense C2.2: A new polyhydroxyalkanoate production candidate.	Lascu I, Mereuta I, Chiciudean I, Hansen H, Avramescu SM, Tanase AM, Stoica I.	Microbiologyopen	10.1002/mbo3.1182	2021
	Microaerobic insights into production of polyhydroxyalkanoates containing 3-hydroxyhexanoate via native reverse beta-oxidation from glucose in Ralstonia eutropha H16.	Huong KH, Orita I, Fukui T.	Microb Cell Fact	10.1186/s12934-024-02294-4	2024
Biotechnology	Utilization of food waste streams for the production of biopolymers.	Ranganathan S, Dutta S, Moses JA, Anandharamakrishnan C.	Heliyon	10.1016/j.heliyon.2020.e04891	2020
	Unlocking efficient polyhydroxyalkanoate production by Gram-positive Priestia megaterium using waste-derived feedstocks.	Bai X, Xu L, Li K, Zhang G, Zhang M, Huang Y.	Microb Cell Fact	10.1186/s12934-025-02803-z	2025
	A fermentation process for the production of poly(3-hydroxybutyrate) using waste cooking oil or waste fish oil as inexpensive carbon substrate.	Loan TT, Trang DTQ, Huy PQ, Ninh PX, Van Thuoc D.	Biotechnol Rep (Amst)	10.1016/j.btre.2022.e00700	2022
	Autotrophic bacterial production of polyhydroxyalkanoates using carbon dioxide as a sustainable carbon source.	Sathiyanarayanan G, Esteves S.	Front Bioeng Biotechnol	10.3389/fbioe.2025.1545438	2025
	De Novo Assembly of the Polyhydroxybutyrate (PHB) Producer Azohydromonas lata Strain H1 Genome and Genomic Analysis of PHB Production Machinery.	Traversa D, Pazzani C, D'Addabbo P, Trisolini L, Chiara M, Oliva M, Marzella A, Mandorino C, Calia C, Chimienti G, Manzari C, Pesole G, Scrascia M.	Microorganisms	10.3390/microorganisms13010137	2025
	Insights into the Degradation of Medium-Chain-Length Dicarboxylic Acids in Cupriavidus necator H16 Reveal beta-Oxidation Differences between Dicarboxylic Acids and Fatty Acids.	Strittmatter CS, Eggers J, Biesgen V, Hengsbach JN, Sakatoku A, Albrecht D, Riedel K, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.01873-21	2022
Metabolism	Applying Statistical Design of Experiments To Understanding the Effect of Growth Medium Components on Cupriavidus necator H16 Growth.	Azubuike CC, Edwards MG, Gatehouse AMR, Howard TP.	Appl Environ Microbiol	10.1128/aem.00705-20	2020
Phylogeny	Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization.	Miu DM, Eremia MC, Moscovici M.	Materials (Basel)	10.3390/ma15041410	2022
	From CO₂ to Bioplastic - Coupling the Electrochemical CO₂ Reduction with a Microbial Product Generation by Drop-in Electrolysis.	Stockl M, Harms S, Dinges I, Dimitrova S, Holtmann D.	ChemSusChem	10.1002/cssc.202001235	2020
	Optimization of a Two-Species Microbial Consortium for Improved Mcl-PHA Production From Glucose-Xylose Mixtures.	Zhu Y, Ai M, Jia X.	Front Bioeng Biotechnol	10.3389/fbioe.2021.794331	2021
	Recent Biotechnological Applications of Polyhydroxyalkanoates (PHA) in the Biomedical Sector-A Review.	Diniz MSDF, Mourao MM, Xavier LP, Santos AV.	Polymers (Basel)	10.3390/polym15224405	2023
	Heterologous phasin expression in Rhodopseudomonas palustris CGA009 for bioplastic production from lignocellulosic biomass.	Brown B, Immethun C, Alsiyabi A, Long D, Wilkins M, Saha R.	Metab Eng Commun	10.1016/j.mec.2021.e00191	2022
Metabolism	Adaptive Laboratory Evolution of Cupriavidus necator H16 for Carbon Co-Utilization with Glycerol.	Gonzalez-Villanueva M, Galaiya H, Staniland P, Staniland J, Savill I, Wong TS, Tee KL.	Int J Mol Sci	10.3390/ijms20225737	2019
	Engineering Cupriavidus necator H16 for the autotrophic production of (R)-1,3-butanediol.	Gascoyne JL, Bommareddy RR, Heeb S, Malys N.	Metab Eng	10.1016/j.ymben.2021.06.010	2021
Metabolism	Untargeted metabolomics analysis of Ralstonia eutropha during plant oil cultivations reveals the presence of a fucose salvage pathway.	Gutschmann B, Bock MCE, Jahns S, Neubauer P, Brigham CJ, Riedel SL.	Sci Rep	10.1038/s41598-021-93720-9	2021
Enzymology	(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha.	Ishihara S, Orita I, Matsumoto K, Fukui T.	Appl Microbiol Biotechnol	10.1007/s00253-023-12797-6	2023
	Optimization of Inulin Hydrolysis by Penicillium lanosocoeruleum Inulinases and Efficient Conversion Into Polyhydroxyalkanoates.	Corrado I, Cascelli N, Ntasi G, Birolo L, Sannia G, Pezzella C.	Front Bioeng Biotechnol	10.3389/fbioe.2021.616908	2021
	Lost in translation: the quest for Nitrosomonas cluster 7-specific amoA primers and TaqMan probes.	Orschler L, Agrawal S, Lackner S.	Microb Biotechnol	10.1111/1751-7915.13627	2020
	Two-Stage Bio-Hydrogen and Polyhydroxyalkanoate Production: Upcycling of Spent Coffee Grounds.	Kang BJ, Jeon JM, Bhatia SK, Kim DH, Yang YH, Jung S, Yoon JJ.	Polymers (Basel)	10.3390/polym15030681	2023
	Functionalization of Polyhydroxyalkanoates (PHA)-Based Bioplastic with Phloretin for Active Food Packaging: Characterization of Its Mechanical, Antioxidant, and Antimicrobial Activities.	Mirpoor SF, Patane GT, Corrado I, Giosafatto CVL, Ginestra G, Nostro A, Foti A, Gucciardi PG, Mandalari G, Barreca D, Gervasi T, Pezzella C.	Int J Mol Sci	10.3390/ijms241411628	2023
	PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003.	Thomas T, Sudesh K, Bazire A, Elain A, Tan HT, Lim H, Bruzaud S.	Bioengineering (Basel)	10.3390/bioengineering7010029	2020
	An Overview of Recent Advancements in Microbial Polyhydroxyalkanoates (PHA) Production from Dark Fermentation Acidogenic Effluents: A Path to an Integrated Bio-Refinery.	Saratale RG, Cho SK, Saratale GD, Kumar M, Bharagava RN, Varjani S, Kadam AA, Ghodake GS, Palem RR, Mulla SI, Kim DS, Shin HS.	Polymers (Basel)	10.3390/polym13244297	2021
Enzymology	Construction and use of a Cupriavidus necator H16 soluble hydrogenase promoter (PSH) fusion to gfp (green fluorescent protein).	Jugder BE, Welch J, Braidy N, Marquis CP.	PeerJ	10.7717/peerj.2269	2016
	Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks.	Vigneswari S, Noor MSM, Amelia TSM, Balakrishnan K, Adnan A, Bhubalan K, Amirul AA, Ramakrishna S.	Life (Basel)	10.3390/life11080807	2021
Metabolism	Food waste conversion to microbial polyhydroxyalkanoates.	Nielsen C, Rahman A, Rehman AU, Walsh MK, Miller CD.	Microb Biotechnol	10.1111/1751-7915.12776	2017
Metabolism	Characterization of highly active 2-keto-3-deoxy-L-arabinonate and 2-keto-3-deoxy-D-xylonate dehydratases in terms of the biotransformation of hemicellulose sugars to chemicals.	Sutiono S, Siebers B, Sieber V.	Appl Microbiol Biotechnol	10.1007/s00253-020-10742-5	2020
	In-Line Monitoring of Polyhydroxyalkanoate (PHA) Production during High-Cell-Density Plant Oil Cultivations Using Photon Density Wave Spectroscopy.	Gutschmann B, Schiewe T, Weiske MTH, Neubauer P, Hass R, Riedel SL.	Bioengineering (Basel)	10.3390/bioengineering6030085	2019
	Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate).	Esmail A, Pereira JR, Sevrin C, Grandfils C, Menda UD, Fortunato E, Oliva A, Freitas F.	Life (Basel)	10.3390/life11090935	2021
	A Novel Nucleic Lateral Flow Assay for Screening phaR-Containing Bacillus spp.	Wint NY, Han KK, Yamprayoonswat W, Ruangsuj P, Mangmool S, Promptmas C, Yasawong M.	J Microbiol Biotechnol	10.4014/jmb.1907.07045	2019
Metabolism	Poly(3-hydroxybutyrate) production in an integrated electromicrobial setup: Investigation under stress-inducing conditions.	Al Rowaihi IS, Paillier A, Rasul S, Karan R, Grotzinger SW, Takanabe K, Eppinger J.	PLoS One	10.1371/journal.pone.0196079	2018
Metabolism	Thermophilic Coenzyme B12-Dependent Acyl Coenzyme A (CoA) Mutase from Kyrpidia tusciae DSM 2912 Preferentially Catalyzes Isomerization of (R)-3-Hydroxybutyryl-CoA and 2-Hydroxyisobutyryl-CoA.	Weichler MT, Kurteva-Yaneva N, Przybylski D, Schuster J, Muller RH, Harms H, Rohwerder T.	Appl Environ Microbiol	10.1128/aem.00716-15	2015
Metabolism	Biotin Synthesis in Ralstonia eutropha H16 Utilizes Pimeloyl Coenzyme A and Can Be Regulated by the Amount of Acceptor Protein.	Eggers J, Strittmatter CS, Kusters K, Biller E, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.01512-20	2020
	Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production.	Bedade DK, Edson CB, Gross RA.	Molecules	10.3390/molecules26113463	2021
Biotechnology	Development of an immunoFET biosensor for the detection of biotinylated PCR product.	Muangsuwan W, Promptmas C, Jeamsaksiri W, Bunjongpru W, Srisuwan A, Hruanun C, Poyai A, Wongchitrat P, Yasawong M.	Heliyon	10.1016/j.heliyon.2016.e00188	2016
Enzymology	Minimal Influence of [NiFe] Hydrogenase on Hydrogen Isotope Fractionation in H₂-Oxidizing Cupriavidus necator.	Campbell BJ, Sessions AL, Fox DN, Paul BG, Qin Q, Kellermann MY, Valentine DL.	Front Microbiol	10.3389/fmicb.2017.01886	2017
Biotechnology	Microbial valorization of underutilized and nonconventional waste streams.	Lad BC, Coleman SM, Alper HS.	J Ind Microbiol Biotechnol	10.1093/jimb/kuab056	2022
	Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production.	Kourmentza C, Placido J, Venetsaneas N, Burniol-Figols A, Varrone C, Gavala HN, Reis MAM.	Bioengineering (Basel)	10.3390/bioengineering4020055	2017
Metabolism	An innovative cloning platform enables large-scale production and maturation of an oxygen-tolerant [NiFe]-hydrogenase from Cupriavidus necator in Escherichia coli.	Schiffels J, Pinkenburg O, Schelden M, Aboulnaga el-HA, Baumann ME, Selmer T.	PLoS One	10.1371/journal.pone.0068812	2013
Enzymology	Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications.	Jugder BE, Lebhar H, Aguey-Zinsou KF, Marquis CP.	MethodsX	10.1016/j.mex.2016.03.005	2016
	Apple orchard waste recycling and valorization of valuable product-A review.	Duan Y, Mehariya S, Kumar A, Singh E, Yang J, Kumar S, Li H, Kumar Awasthi M.	Bioengineered	10.1080/21655979.2021.1872905	2021
Metabolism	Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds.	Zhang M, Kurita S, Orita I, Nakamura S, Fukui T.	Microb Cell Fact	10.1186/s12934-019-1197-7	2019
	Polyhydroxyalkanoates-Based Nanoparticles as Essential Oil Carriers.	Corrado I, Di Girolamo R, Regalado-Gonzalez C, Pezzella C.	Polymers (Basel)	10.3390/polym14010166	2022
Metabolism	Sulfoacetate is degraded via a novel pathway involving sulfoacetyl-CoA and sulfoacetaldehyde in Cupriavidus necator H16.	Weinitschke S, Hollemeyer K, Kusian B, Bowien B, Smits TH, Cook AM.	J Biol Chem	10.1074/jbc.m110.127043	2010
Metabolism	Homotaurine metabolized to 3-sulfopropanoate in Cupriavidus necator H16: enzymes and genes in a patchwork pathway.	Mayer J, Cook AM.	J Bacteriol	10.1128/jb.00678-09	2009
	Oxygen Plasma Treated-Electrospun Polyhydroxyalkanoate Scaffolds for Hydrophilicity Improvement and Cell Adhesion.	Esmail A, Pereira JR, Zoio P, Silvestre S, Menda UD, Sevrin C, Grandfils C, Fortunato E, Reis MAM, Henriques C, Oliva A, Freitas F.	Polymers (Basel)	10.3390/polym13071056	2021
	Determination of Polyhydroxybutyrate (PHB) Content in Ralstonia eutropha Using Gas Chromatography and Nile Red Staining.	Juengert JR, Bresan S, Jendrossek D.	Bio Protoc	10.21769/bioprotoc.2748	2018
	Potential and Prospects of Continuous Polyhydroxyalkanoate (PHA) Production.	Koller M, Braunegg G.	Bioengineering (Basel)	10.3390/bioengineering2020094	2015
Metabolism	Autotrophic production of stable-isotope-labeled arginine in Ralstonia eutropha strain H16.	Lutte S, Pohlmann A, Zaychikov E, Schwartz E, Becher JR, Heumann H, Friedrich B.	Appl Environ Microbiol	10.1128/aem.01972-12	2012
Metabolism	(S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB') from fatty acid degradation operon of Ralstonia eutropha H16.	Volodina E, Steinbuchel A.	AMB Express	10.1186/s13568-014-0069-0	2014
Metabolism	Dihydrolipoamide dehydrogenases of Advenella mimigardefordensis and Ralstonia eutropha catalyze cleavage of 3,3'-dithiodipropionic acid into 3-mercaptopropionic acid.	Wubbeler JH, Raberg M, Brandt U, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.01706-10	2010
Metabolism	Impact of Ralstonia eutropha's poly(3-Hydroxybutyrate) (PHB) Depolymerases and Phasins on PHB storage in recombinant Escherichia coli.	Eggers J, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.02666-14	2014
Metabolism	Hydrogen-driven asymmetric reduction of hydroxyacetone to (R)-1,2-propanediol by Ralstonia eutropha transformant expressing alcohol dehydrogenase from Kluyveromyces lactis.	Oda T, Oda K, Yamamoto H, Matsuyama A, Ishii M, Igarashi Y, Nishihara H.	Microb Cell Fact	10.1186/1475-2859-12-2	2013
Metabolism	A closer look on the polyhydroxybutyrate- (PHB-) negative phenotype of Ralstonia eutropha PHB-4.	Raberg M, Voigt B, Hecker M, Steinbuchel A.	PLoS One	10.1371/journal.pone.0095907	2014
Metabolism	CO synthesized from the central one-carbon pool as source for the iron carbonyl in O2-tolerant [NiFe]-hydrogenase.	Burstel I, Siebert E, Frielingsdorf S, Zebger I, Friedrich B, Lenz O.	Proc Natl Acad Sci U S A	10.1073/pnas.1614656113	2016
Metabolism	Genetically modified strains of Ralstonia eutropha H16 with beta-ketothiolase gene deletions for production of copolyesters with defined 3-hydroxyvaleric acid contents.	Lindenkamp N, Volodina E, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.00824-12	2012
Metabolism	The maturation factors HoxR and HoxT contribute to oxygen tolerance of membrane-bound [NiFe] hydrogenase in Ralstonia eutropha H16.	Fritsch J, Lenz O, Friedrich B.	J Bacteriol	10.1128/jb.01427-10	2011
Metabolism	Extension of the substrate utilization range of Ralstonia eutropha strain H16 by metabolic engineering to include mannose and glucose.	Sichwart S, Hetzler S, Broker D, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.01977-10	2011
Metabolism	Poly(3-hydroxybutyrate) degradation in Ralstonia eutropha H16 is mediated stereoselectively to (S)-3-hydroxybutyryl coenzyme A (CoA) via crotonyl-CoA.	Eggers J, Steinbuchel A.	J Bacteriol	10.1128/jb.00358-13	2013
Metabolism	Impact of multiple beta-ketothiolase deletion mutations in Ralstonia eutropha H16 on the composition of 3-mercaptopropionic acid-containing copolymers.	Lindenkamp N, Peplinski K, Volodina E, Ehrenreich A, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.01058-10	2010
Metabolism	New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate.	York GM, Stubbe J, Sinskey AJ.	J Bacteriol	10.1128/jb.183.7.2394-2397.2001	2001
Metabolism	Characterization of the reduction of selenate and tellurite by nitrate reductases.	Sabaty M, Avazeri C, Pignol D, Vermeglio A.	Appl Environ Microbiol	10.1128/aem.67.11.5122-5126.2001	2001
Metabolism	The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production.	York GM, Stubbe J, Sinskey AJ.	J Bacteriol	10.1128/jb.184.1.59-66.2002	2002
Metabolism	Functional analysis by site-directed mutagenesis of the NAD(+)-reducing hydrogenase from Ralstonia eutropha.	Burgdorf T, De Lacey AL, Friedrich B.	J Bacteriol	10.1128/jb.184.22.6280-6288.2002	2002
Metabolism	Carbonic anhydrase is essential for growth of Ralstonia eutropha at ambient CO(2) concentrations.	Kusian B, Sultemeyer D, Bowien B.	J Bacteriol	10.1128/jb.184.18.5018-5026.2002	2002
Enzymology	A megaplasmid-borne anaerobic ribonucleotide reductase in Alcaligenes eutrophus H16.	Siedow A, Cramm R, Siddiqui RA, Friedrich B.	J Bacteriol	10.1128/jb.181.16.4919-4928.1999	1999
Metabolism	rRNA and poly-beta-hydroxybutyrate dynamics in bioreactors subjected to feast and famine cycles.	Frigon D, Muyzer G, van Loosdrecht M, Raskin L.	Appl Environ Microbiol	10.1128/aem.72.4.2322-2330.2006	2006
Metabolism	The soluble NAD+-Reducing [NiFe]-hydrogenase from Ralstonia eutropha H16 consists of six subunits and can be specifically activated by NADPH.	Burgdorf T, van der Linden E, Bernhard M, Yin QY, Back JW, Hartog AF, Muijsers AO, de Koster CG, Albracht SP, Friedrich B.	J Bacteriol	10.1128/jb.187.9.3122-3132.2005	2005
Metabolism	Proteomic and transcriptomic elucidation of the mutant ralstonia eutropha G+1 with regard to glucose utilization.	Raberg M, Peplinski K, Heiss S, Ehrenreich A, Voigt B, Doring C, Bomeke M, Hecker M, Steinbuchel A.	Appl Environ Microbiol	10.1128/aem.02015-10	2011
Metabolism	Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells.	York GM, Junker BH, Stubbe JA, Sinskey AJ.	J Bacteriol	10.1128/jb.183.14.4217-4226.2001	2001
Metabolism	Insights into genetic determinants of volatile fatty acid catabolism in Cupriavidus necator H16.	Holmes EC, Breunig SL, Johnson CW, Beckham GT, Bleem AC.	Appl Environ Microbiol	10.1128/aem.00515-25	2025
	Production of polyhydroxyalkanoate (PHA) biopolymer from crop residue using bacteria as an alternative to plastics: a review.	Chouhan A, Tiwari A.	RSC Adv	10.1039/d4ra08505a	2025
	Isopropanol production from carbon dioxide by Cupriavidus necator using a zero-gap cell with culture broth as catholyte	Schoenmakers P, Rad R, Ihl A, Weickardt I, Guillouet S, Apfel U, Lauterbach L.	iScience		2025
	CO₂ upgrading into bioproducts using a two-step abiotic-biotic system.	Lee G, Jo HJ, Choi J, Guzman MF, Shan Y, Le HKD, Feijoo J, Soland N, Clark DS, Yang P.	Proc Natl Acad Sci U S A	10.1073/pnas.2512565122	2025
	Identification of Oil-Loving Cupriavidus necator BM3-1 for Polyhydroxyalkanoate Production and Assessing Contribution of Exopolysaccharide for Vegetable Oil Utilization	Shin Y, Kim H, Choi T, Oh S, Kim S, Lee Y, Choi S, Oh J, Kim S, Lee Y, Choi Y, Bhatia S, Yang Y.	Polymers (Basel)		2024
	Biorecycling of polyethylene (PE): an integrated effort in pretreatment, degradation, and upcycling.	Abid U, Gibbons J, Qin J, Xie D.	Front Bioeng Biotechnol	10.3389/fbioe.2025.1692651	2025
	Genotypic and Phenotypic Detection of Polyhydroxyalkanoate Production in Bacterial Isolates from Food.	Macalova D, Janalikova M, Sedlarikova J, Rektorikova I, Koutny M, Pleva P.	Int J Mol Sci	10.3390/ijms24021250	2023
	Biosynthesis and Properties of a P(3HB-co-3HV-co-4HV) Produced by Cupriavidus necator B-10646.	Zhila NO, Sapozhnikova KY, Kiselev EG, Nemtsev IV, Lukyanenko AV, Shishatskaya EI, Volova TG.	Polymers (Basel)	10.3390/polym14194226	2022
	Polyhydroxybutyrate production by freshwater SAR11 (LD12).	Bennett BD, Meier DAO, Lanclos VC, Asrari H, Coates JD, Thrash JC.	ISME J	10.1093/ismejo/wraf087	2025
	Production of polyhydroxybutyrate by coupled saccharification-fermentation of inulin.	Guzman-Lagunes F, Martinez-dlCruz L, Wongsirichot P, Winterburn J, Montiel C.	Bioprocess Biosyst Eng	10.1007/s00449-023-02953-7	2024
	Sugar Beet Molasses as a Potential C-Substrate for PHA Production by Cupriavidus necator.	Kiselev EG, Demidenko AV, Zhila NO, Shishatskaya EI, Volova TG.	Bioengineering (Basel)	10.3390/bioengineering9040154	2022
Biotechnology	Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria.	Keating KW, Young EM.	ACS Synth Biol	10.1021/acssynbio.3c00104	2023
	Poly(3-hydroxybutyrate) Production from Lignocellulosic Wastes Using Bacillus megaterium ATCC 14581.	Senila L, Gal E, Kovacs E, Cadar O, Dan M, Senila M, Roman C.	Polymers (Basel)	10.3390/polym15234488	2023
	Engineering a Rhodopsin-Based Photo-Electrosynthetic System in Bacteria for CO₂ Fixation.	Davison PA, Tu W, Xu J, Della Valle S, Thompson IP, Hunter CN, Huang WE.	ACS Synth Biol	10.1021/acssynbio.2c00397	2022
	Proteomic Examination for Gluconeogenesis Pathway-Shift during Polyhydroxyalkanoate Formation in Cupriavidus necator Grown on Glycerol.	Tanadchangsaeng N, Roytrakul S.	Bioengineering (Basel)	10.3390/bioengineering7040154	2020
	Biotransformation of d-Xylose-Rich Rice Husk Hydrolysate by a Rice Paddy Soil Bacterium, Priestia sp. Strain JY310, to Low Molecular Weight Poly(3-hydroxybutyrate).	Lee JY, Kim MH, Kim JS, Yun BR, Kim DY, Chung CW.	Biomolecules	10.3390/biom13010131	2023
	Bioconversion Process of Polyethylene from Waste Tetra Pak^® Packaging to Polyhydroxyalkanoates.	Ekere I, Johnston B, Tchuenbou-Magaia F, Townrow D, Wojciechowski S, Marek A, Zawadiak J, Duale K, Zieba M, Sikorska W, Adamus G, Goslar T, Kowalczuk M, Radecka I.	Polymers (Basel)	10.3390/polym14142840	2022
	Improved fermentation strategies in a bioreactor for enhancing poly(3-hydroxybutyrate) (PHB) production by wild type Cupriavidus necator from fructose.	Nygaard D, Yashchuk O, Noseda DG, Araoz B, Hermida EB.	Heliyon	10.1016/j.heliyon.2021.e05979	2021
	Biosynthetic Plastics as Tunable Elastic and Visible Stent with Shape-Memory to Treat Biliary Stricture.	Wang W, Luan Z, Shu Z, Xu K, Wang T, Liu S, Wu X, Liu H, Ye S, Dan R, Zhao X, Yang S, Xing M, Fan C.	Adv Sci (Weinh)	10.1002/advs.202303779	2023
Phylogeny	Producing and Characterizing Polyhydroxyalkanoates from Starch and Chickpea Waste Using Mixed Microbial Cultures in Solid-State Fermentation.	Grgurevic K, Bramberger D, Miloloza M, Stublic K, Ocelic Bulatovic V, Ranilovic J, Ukic S, Bolanca T, Cvetnic M, Markic M, Kucic Grgic D.	Polymers (Basel)	10.3390/polym16233407	2024
Metabolism	Pretreatment of kenaf (Hibiscus cannabinus L.) biomass feedstock for polyhydroxybutyrate (PHB) production and characterization.	Saratale RG, Saratale GD, Cho SK, Kim DS, Ghodake GS, Kadam A, Kumar G, Bharagava RN, Banu R, Shin HS.	Bioresour Technol	10.1016/j.biortech.2019.02.083	2019
Metabolism	In vivo immobilization of an organophosphorus hydrolyzing enzyme on bacterial polyhydroxyalkanoate nano-granules.	Li R, Yang J, Xiao Y, Long L.	Microb Cell Fact	10.1186/s12934-019-1201-2	2019
	Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production.	Szacherska K, Oleskowicz-Popiel P, Ciesielski S, Mozejko-Ciesielska J.	Polymers (Basel)	10.3390/polym13030321	2021
	CRISPR-COPIES: an in silico platform for discovery of neutral integration sites for CRISPR/Cas-facilitated gene integration.	Boob AG, Zhu Z, Intasian P, Jain M, Petrov VA, Lane ST, Tan SI, Xun G, Zhao H.	Nucleic Acids Res	10.1093/nar/gkae062	2024
	Continuous Supply of Non-Combustible Gas Mixture for Safe Autotrophic Culture to Produce Polyhydroxyalkanoate by Hydrogen-Oxidizing Bacteria.	Miyahara Y, Wang CT, Ishii-Hyakutake M, Tsuge T.	Bioengineering (Basel)	10.3390/bioengineering9100586	2022
	Enhanced production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer by endophytic Bacillus cereus RCL 02 utilizing sugarcane molasses as sole source of carbon: a statistical optimization approach.	Das R, Pal A, Paul AK.	BioTechnologia (Pozn)	10.5114/bta.2022.118671	2022
Metabolism	Discovery of novel pathways for carbohydrate metabolism.	Stack TMM, Gerlt JA.	Curr Opin Chem Biol	10.1016/j.cbpa.2020.09.005	2021
	Enhancement of bioplastic polyhydroxybutyrate P(3HB) production from glucose by newly engineered strain Cupriavidus necator NSDG-GG using response surface methodology.	Biglari N, Ganjali Dashti M, Abdeshahian P, Orita I, Fukui T, Sudesh K.	3 Biotech	10.1007/s13205-018-1351-7	2018
	Conversion of Short and Medium Chain Fatty Acids into Novel Polyhydroxyalkanoates Copolymers by Aeromonas sp. AC_01.	Szacherska K, Moraczewski K, Czaplicki S, Oleskowicz-Popiel P, Mozejko-Ciesielska J.	Materials (Basel)	10.3390/ma15134482	2022
Metabolism	Cupriavidus necator H16 Uses Flavocytochrome c Sulfide Dehydrogenase To Oxidize Self-Produced and Added Sulfide.	Lu C, Xia Y, Liu D, Zhao R, Gao R, Liu H, Xun L.	Appl Environ Microbiol	10.1128/aem.01610-17	2017
	Comparison of different solvents for extraction of polyhydroxybutyrate from Cupriavidus necator.	Aramvash A, Moazzeni Zavareh F, Gholami Banadkuki N.	Eng Life Sci	10.1002/elsc.201700102	2018
	The use of low-cost brewery waste product for the production of surfactin as a natural microbial biocide.	Nazareth TC, Zanutto CP, Tripathi L, Juma A, Maass D, de Souza AAU, de Arruda Guelli Ulson de Souza SM, Banat IM.	Biotechnol Rep (Amst)	10.1016/j.btre.2020.e00537	2020
	Improved fed-batch production of high-purity PHB (poly-3 hydroxy butyrate) by Cupriavidus necator (MTCC 1472) from sucrose-based cheap substrates under response surface-optimized conditions.	Dey P, Rangarajan V.	3 Biotech	10.1007/s13205-017-0948-6	2017
	Using Humidity to Control the Morphology and Properties of Electrospun BioPEGylated Polyhydroxybutyrate Scaffolds.	Foster LJR, Chan RTH, Russell RA, Holden PJ.	ACS Omega	10.1021/acsomega.0c02993	2020
Metabolism	Engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using unrelated carbon sources.	Srirangan K, Liu X, Tran TT, Charles TC, Moo-Young M, Chou CP.	Sci Rep	10.1038/srep36470	2016
	Prospecting for Marine Bacteria for Polyhydroxyalkanoate Production on Low-Cost Substrates.	Takahashi RYU, Castilho NAS, Silva MACD, Miotto MC, Lima AOS.	Bioengineering (Basel)	10.3390/bioengineering4030060	2017
	A comparative analysis of biopolymer production by microbial and bioelectrochemical technologies.	Alvarez Chavez B, Raghavan V, Tartakovsky B.	RSC Adv	10.1039/d1ra08796g	2022
	Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments.	Johnston B, Radecka I, Chiellini E, Barsi D, Ilieva VI, Sikorska W, Musiol M, Zieba M, Chaber P, Marek AA, Mendrek B, Ekere AI, Adamus G, Kowalczuk M.	Polymers (Basel)	10.3390/polym11101580	2019
Metabolism	Global changes in the proteome of Cupriavidus necator H16 during poly-(3-hydroxybutyrate) synthesis from various biodiesel by-product substrates.	Sharma PK, Fu J, Spicer V, Krokhin OV, Cicek N, Sparling R, Levin DB.	AMB Express	10.1186/s13568-016-0206-z	2016
	The Molecular Level Characterization of Biodegradable Polymers Originated from Polyethylene Using Non-Oxygenated Polyethylene Wax as a Carbon Source for Polyhydroxyalkanoate Production.	Johnston B, Jiang G, Hill D, Adamus G, Kwiecien I, Zieba M, Sikorska W, Green M, Kowalczuk M, Radecka I.	Bioengineering (Basel)	10.3390/bioengineering4030073	2017
	An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture.	Jugder BE, Chen Z, Ping DT, Lebhar H, Welch J, Marquis CP.	Microb Cell Fact	10.1186/s12934-015-0226-4	2015
	Integrated systems for biopolymers and bioenergy production from organic waste and by-products: a review of microbial processes.	Pagliano G, Ventorino V, Panico A, Pepe O.	Biotechnol Biofuels	10.1186/s13068-017-0802-4	2017
	Production of polyhydroxyalkanoates from waste frying oil by Cupriavidus necator.	Verlinden RA, Hill DJ, Kenward MA, Williams CD, Piotrowska-Seget Z, Radecka IK.	AMB Express	10.1186/2191-0855-1-11	2011
	Beyond Intracellular Accumulation of Polyhydroxyalkanoates: Chiral Hydroxyalkanoic Acids and Polymer Secretion.	Yanez L, Conejeros R, Vergara-Fernandez A, Scott F.	Front Bioeng Biotechnol	10.3389/fbioe.2020.00248	2020
	Induction of Systemic Resistance in Maize and Antibiofilm Activity of Surfactin From Bacillus velezensis MS20.	Ali SAM, Sayyed RZ, Mir MI, Khan MY, Hameeda B, Alkhanani MF, Haque S, Mohammad Al Tawaha AR, Poczai P.	Front Microbiol	10.3389/fmicb.2022.879739	2022
	The Microbial Production of Polyhydroxyalkanoates from Waste Polystyrene Fragments Attained Using Oxidative Degradation.	Johnston B, Radecka I, Hill D, Chiellini E, Ilieva VI, Sikorska W, Musiol M, Zieba M, Marek AA, Keddie D, Mendrek B, Darbar S, Adamus G, Kowalczuk M.	Polymers (Basel)	10.3390/polym10090957	2018
Genetics	Mining of Microbial Genomes for the Novel Sources of Nitrilases.	Sharma N, Thakur N, Raj T, Savitri, Bhalla TC.	Biomed Res Int	10.1155/2017/7039245	2017
Enzymology	Efficient production of active polyhydroxyalkanoate synthase in Escherichia coli by coexpression of molecular chaperones.	Thomson NM, Thomson NM, Saika A, Ushimaru K, Sangiambut S, Tsuge T, Summers DK, Sivaniah E.	Appl Environ Microbiol	10.1128/aem.02881-12	2013
	Targeted and high-throughput gene knockdown in diverse bacteria using synthetic sRNAs.	Cho JS, Yang D, Prabowo CPS, Ghiffary MR, Han T, Choi KR, Moon CW, Zhou H, Ryu JY, Kim HU, Lee SY.	Nat Commun	10.1038/s41467-023-38119-y	2023
	Polyhydroxyalkanoates (PHAs): Biopolymers for Biofuel and Biorefineries.	Riaz S, Rhee KY, Rhee KY, Park SJ.	Polymers (Basel)	10.3390/polym13020253	2021
Metabolism	Engineering Escherichia coli for Microbial Production of Butanone.	Srirangan K, Liu X, Akawi L, Bruder M, Moo-Young M, Chou CP.	Appl Environ Microbiol	10.1128/aem.03964-15	2016
Metabolism	Revisiting the single cell protein application of Cupriavidus necator H16 and recovering bioplastic granules simultaneously.	Kunasundari B, Murugaiyah V, Kaur G, Maurer FH, Sudesh K.	PLoS One	10.1371/journal.pone.0078528	2013
	A Transcription Factor-Based Biosensor for Detection of Itaconic Acid.	Hanko EKR, Minton NP, Malys N.	ACS Synth Biol	10.1021/acssynbio.8b00057	2018
Metabolism	RubisCO selection using the vigorously aerobic and metabolically versatile bacterium Ralstonia eutropha.	Satagopan S, Tabita FR.	FEBS J	10.1111/febs.13774	2016
Biotechnology	The role of biotechnology in the transition from plastics to bioplastics: an opportunity to reconnect global growth with sustainability.	Degli Esposti M, Morselli D, Fava F, Bertin L, Cavani F, Viaggi D, Fabbri P.	FEBS Open Bio	10.1002/2211-5463.13119	2021
Metabolism	Response surface method for polyhydroxybutyrate (PHB) bioplastic accumulation in Bacillus drentensis BP17 using pineapple peel.	Penkhrue W, Jendrossek D, Khanongnuch C, Pathom-Aree W, Aizawa T, Behrens RL, Lumyong S.	PLoS One	10.1371/journal.pone.0230443	2020
	Secretion of polyhydroxybutyrate in Escherichia coli using a synthetic biological engineering approach.	Rahman A, Linton E, Hatch AD, Sims RC, Miller CD.	J Biol Eng	10.1186/1754-1611-7-24	2013
Metabolism	Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work.	Pena C, Castillo T, Garcia A, Millan M, Segura D.	Microb Biotechnol	10.1111/1751-7915.12129	2014
	Production and Characterization of Polyhydroxyalkanoate from Lignin Derivatives by Pandoraea sp. ISTKB.	Kumar M, Singhal A, Verma PK, Thakur IS.	ACS Omega	10.1021/acsomega.7b01615	2017
Metabolism	Delineation of Steroid-Degrading Microorganisms through Comparative Genomic Analysis.	Bergstrand LH, Cardenas E, Holert J, Van Hamme JD, Mohn WW.	mBio	10.1128/mbio.00166-16	2016
Metabolism	Kinetic and stoichiometric characterization of organoautotrophic growth of Ralstonia eutropha on formic acid in fed-batch and continuous cultures.	Grunwald S, Mottet A, Grousseau E, Plassmeier JK, Popovic MK, Uribelarrea JL, Gorret N, Guillouet SE, Sinskey A.	Microb Biotechnol	10.1111/1751-7915.12149	2015
	Surface Active Agents and Their Health-Promoting Properties: Molecules of Multifunctional Significance.	Anestopoulos I, Kiousi DE, Klavaris A, Galanis A, Salek K, Euston SR, Pappa A, Panayiotidis MI.	Pharmaceutics	10.3390/pharmaceutics12070688	2020
Pathogenicity	PHA productivity and yield of Ralstonia eutropha when intermittently or continuously fed a mixture of short chain fatty acids.	Chakraborty P, Muthukumarappan K, Gibbons WR.	J Biomed Biotechnol	10.1155/2012/506153	2012
Metabolism	Metabolic engineering of Escherichia coli for production of enantiomerically pure (R)-(--)-hydroxycarboxylic acids.	Lee SY, Lee Y.	Appl Environ Microbiol	10.1128/aem.69.6.3421-3426.2003	2003
	Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16.	Gai CS, Lu J, Brigham CJ, Bernardi AC, Sinskey AJ.	AMB Express	10.1186/2191-0855-4-2	2014
Metabolism	Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16.	Budde CF, Mahan AE, Lu J, Rha C, Sinskey AJ.	J Bacteriol	10.1128/jb.00207-10	2010
Metabolism	Purification and properties of an intracellular 3-hydroxybutyrate-oligomer hydrolase (PhaZ2) in Ralstonia eutropha H16 and its identification as a novel intracellular poly(3-hydroxybutyrate) depolymerase.	Kobayashi T, Shiraki M, Abe T, Sugiyama A, Saito T.	J Bacteriol	10.1128/jb.185.12.3485-3490.2003	2003
Metabolism	Requirements for heterologous production of a complex metalloenzyme: the membrane-bound [NiFe] hydrogenase.	Lenz O, Gleiche A, Strack A, Friedrich B.	J Bacteriol	10.1128/jb.187.18.6590-6595.2005	2005
Metabolism	Spectroscopic and Kinetic Properties of the Molybdenum-containing, NAD+-dependent Formate Dehydrogenase from Ralstonia eutropha.	Niks D, Duvvuru J, Escalona M, Hille R.	J Biol Chem	10.1074/jbc.m115.688457	2016
Metabolism	Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains.	Budde CF, Riedel SL, Willis LB, Rha C, Sinskey AJ.	Appl Environ Microbiol	10.1128/aem.02429-10	2011
	From Residues to Added-Value Bacterial Biopolymers as Nanomaterials for Biomedical Applications.	Blanco FG, Hernandez N, Rivero-Buceta V, Maestro B, Sanz JM, Mato A, Hernandez-Arriaga AM, Prieto MA.	Nanomaterials (Basel)	10.3390/nano11061492	2021
Metabolism	Cloning of an intracellular Poly[D(-)-3-Hydroxybutyrate] depolymerase gene from Ralstonia eutropha H16 and characterization of the gene product.	Saegusa H, Shiraki M, Kanai C, Saito T.	J Bacteriol	10.1128/jb.183.1.94-100.2001	2001
Metabolism	Properties of a novel intracellular poly(3-hydroxybutyrate) depolymerase with high specific activity (PhaZd) in Wautersia eutropha H16.	Abe T, Kobayashi T, Saito T.	J Bacteriol	10.1128/jb.187.20.6982-6990.2005	2005
Metabolism	Characterization of the signaling domain of the NO-responsive regulator NorR from Ralstonia eutropha H16 by site-directed mutagenesis.	Klink A, Elsner B, Strube K, Cramm R.	J Bacteriol	10.1128/jb.01865-06	2007
Metabolism	Involvement of hyp gene products in maturation of the H(2)-sensing [NiFe] hydrogenase of Ralstonia eutropha.	Buhrke T, Bleijlevens B, Albracht SP, Friedrich B.	J Bacteriol	10.1128/jb.183.24.7087-7093.2001	2001
Metabolism	The H(2) sensor of Ralstonia eutropha is a member of the subclass of regulatory [NiFe] hydrogenases.	Kleihues L, Lenz O, Bernhard M, Buhrke T, Friedrich B.	J Bacteriol	10.1128/jb.182.10.2716-2724.2000	2000
Metabolism	Ralstonia eutropha TF93 is blocked in tat-mediated protein export.	Bernhard M, Friedrich B, Siddiqui RA.	J Bacteriol	10.1128/jb.182.3.581-588.2000	2000
Metabolism	Mutational analysis of the cbb operon (CO2 assimilation) promoter of Ralstonia eutropha.	Jeffke T, Gropp NH, Kaiser C, Grzeszik C, Kusian B, Bowien B.	J Bacteriol	10.1128/jb.181.14.4374-4380.1999	1999
Metabolism	Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic.	Madison LL, Huisman GW.	Microbiol Mol Biol Rev	10.1128/mmbr.63.1.21-53.1999	1999
Biotechnology	Developing Microbial Co-Culture System for Enhanced Polyhydroxyalkanoates (PHA) Production Using Acid Pretreated Lignocellulosic Biomass.	Saratale RG, Cho SK, Kadam AA, Ghodake GS, Kumar M, Bharagava RN, Varjani S, Nair S, Kim DS, Shin HS, Saratale GD	Polymers (Basel)	10.3390/polym14040726	2022
	Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer.	Pantelic B, Ponjavic M, Jankovic V, Aleksic I, Stevanovic S, Murray J, Fournet MB, Nikodinovic-Runic J	Polymers (Basel)	10.3390/polym13213692	2021
Biotechnology	Utilization of Noxious Weed Water Hyacinth Biomass as a Potential Feedstock for Biopolymers Production: A Novel Approach.	Saratale RG, Cho SK, Ghodake GS, Shin HS, Saratale GD, Park Y, Lee HS, Bharagava RN, Kim DS	Polymers (Basel)	10.3390/polym12081704	2020
	Preparation and Characterization of Films Based on a Natural P(3HB)/mcl-PHA Blend Obtained through the Co-culture of Cupriavidus Necator and Pseudomonas Citronellolis in Apple Pulp Waste.	Rebocho AT, Pereira JR, Neves LA, Alves VD, Sevrin C, Grandfils C, Freitas F, Reis MAM	Bioengineering (Basel)	10.3390/bioengineering7020034	2020
Metabolism	Characterization of an l-Ascorbate Catabolic Pathway with Unprecedented Enzymatic Transformations.	Stack TMM, Morrison KN, Dettmer TM, Wille B, Kim C, Joyce R, Jermain M, Naing YT, Bhatti K, Francisco BS, Carter MS, Gerlt JA	J Am Chem Soc	10.1021/jacs.9b09863	2020
Genetics	Complete Genome Sequence of Cupriavidus necator H16 (DSM 428).	Little GT, Ehsaan M, Arenas-Lopez C, Jawed K, Winzer K, Kovacs K, Minton NP	Microbiol Resour Announc	10.1128/MRA.00814-19	2019
Metabolism	Characterization of poly-3-hydroxybutyrate (PHB) produced from Ralstonia eutropha using an alkali-pretreated biomass feedstock.	Saratale GD, Oh MK	Int J Biol Macromol	10.1016/j.ijbiomac.2015.07.034	2015
Pathogenicity	Sophorolipid biosurfactants: Possible uses as antibacterial and antibiofilm agent.	Diaz De Rienzo MA, Banat IM, Dolman B, Winterburn J, Martin PJ	N Biotechnol	10.1016/j.nbt.2015.02.009	2015
Metabolism	Recovery of amorphous polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil.	Martino L, Cruz MV, Scoma A, Freitas F, Bertin L, Scandola M, Reis MA	Int J Biol Macromol	10.1016/j.ijbiomac.2014.04.016	2014
Biotechnology	Production of polyhydroxyalkanoates from spent coffee grounds oil obtained by supercritical fluid extraction technology.	Cruz MV, Paiva A, Lisboa P, Freitas F, Alves VD, Simoes P, Barreiros S, Reis MA	Bioresour Technol	10.1016/j.biortech.2014.02.013	2014
Metabolism	Molecular genetics and biochemistry of N-acetyltaurine degradation by Cupriavidus necator H16.	Denger K, Lehmann S, Cook AM	Microbiology (Reading)	10.1099/mic.0.048462-0	2011
Metabolism	Reaction engineering studies for the production of 2-hydroxyisobutyric acid with recombinant Cupriavidus necator H 16.	Hoefel T, Wittmann E, Reinecke L, Weuster-Botz D	Appl Microbiol Biotechnol	10.1007/s00253-010-2739-4	2010
Metabolism	Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures.	Wang J, Yu HQ	Appl Microbiol Biotechnol	10.1007/s00253-007-0870-7	2007
Metabolism	Identification of oxalotrophic bacteria by neural network analysis of numerical phenetic data.	Sahin N, Aydin S	Folia Microbiol (Praha)	10.1007/BF02932161	2006
Enzymology	Stability and activity of hydrogenases of Methanobacterium thermoautotrophicum and Alcaligenes eutrophus in reversed micellar systems.	Hoppert M, Braks I, Mayer F	FEMS Microbiol Lett	10.1111/j.1574-6968.1994.tb06836.x	1994
Enzymology	Rhein as an electron acceptor for various flavoproteins and for electron transport particles.	Egerer P, Buhler M, Simon H	Hoppe Seylers Z Physiol Chem	10.1515/bchm2.1982.363.1.627	1982
Phylogeny	Isolation and characterization of polysheaths, phage tail-like defective bacteriophages of Alcaligenes eutrophus H 16.	Walther-Mauruschat A, Mayer F	J Gen Virol	10.1099/0022-1317-41-2-239	1978
Metabolism	Mutants of Alcaligenes eutrophus defective in autotrophic metabolism.	Schink B, Schlegel HG	Arch Microbiol	10.1007/BF00402299	1978
Enzymology	Alpha-isopropylmalate synthase from Alcaligenes eutrophus H 16. III. Endproduct inhibition and its relief by valine and isoleucine.	Wiegel J, Schlegel HG	Arch Microbiol	10.1007/BF00446863	1977
Enzymology	alpha-Isopropylmalate synthase from Alcaligenes eutrophus H 16. II. Substrate specificity and kinetics.	Wiegel J, Schlegel HG	Arch Microbiol	10.1007/BF00413088	1977
Enzymology	Localization and stability of hydrogenases from aerobic hydrogen bacteria.	Schneider K, Schlegel HG	Arch Microbiol	10.1007/BF00413086	1977
Enzymology	Alpha-Isopropylmalate synthase from Alcaligenes eutrophus H 16 I. Purification and general properties.	Wiegel J, Schlegel HG	Arch Microbiol	10.1007/BF00413087	1977
Enzymology	Purification and properties of soluble hydrogenase from Alcaligenes eutrophus H 16.	Schneider K, Schlegel HG	Biochim Biophys Acta	10.1016/0005-2744(76)90058-9	1976
Metabolism	Regulation of Chorismate mutase-prephenate dehydratase and prephenate dehydrogenase from alcaligenes eutrophus.	Friedrich CG, Friedrich B, Schlegel HG	J Bacteriol	10.1128/jb.126.2.723-732.1976	1976
Enzymology	Aromatic amino acid biosynthesis in Alcaligenes eutrophus H 16 III. Properites and regulation of anthranilate synthase.	Friedrich CG, Friedrich B, Schlegel HG	Arch Microbiol	10.1007/BF00446831	1976
Metabolism	[Adenosine-dependent death of Hydrogenomonas eutropha (Alcaligenes eutrophus) H 16 (author's transl)].	Kaltwasser H, Glaeser H	Zentralbl Bakteriol Parasitenkd Infektionskr Hyg	10.1016/s0044-4057(76)80055-x	1976
Enzymology	Regulation of the pyruvate kinase from Alcaligenes eutrophus H 16 in vitro and in vivo.	Wilke D, Schlegel HG	Arch Microbiol	10.1007/BF00447123	1975
Metabolism	Aromatic amino acid biosynthesis in Alcaligenes eutrophus H16. II. The isolation and characterization of mutants auxotrophic for phenylalanine and tyrosine.	Friedrich B, Schlegel HG	Arch Microbiol	10.1007/BF00436341	1975
Enzymology	Kinetics and properties of beta-ketothiolase from Clostridium pasteurianum.	Berndt H, Schlegel HG	Arch Microbiol	10.1007/BF00436325	1975
Biotechnology	Monitoring poly(3-hydroxybutyrate) production in cupriavidus necator DSM 428 (H16) with raman spectroscopy.	Gelder JD, Willemse-Erix D, Scholtes MJ, Sanchez JI, Maquelin K, Vandenabeele P, Boever PD, Puppels GJ, Moens L, Vos PD	Anal Chem	10.1021/ac702185d	2008

References

#197	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 428
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Cupriavidus necator (DSM 428, ATCC 17699, KCTC 22496)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_004798725

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media