Name: Cupriavidus necator H850-4LT
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2032

Type strain:

Species: Cupriavidus necator

Strain Designation: H850-4LT

Culture col. no.: DSM 13439

Strain history: <- R M. Wittich, GBF, genetic modification <- NRRL

NCBI tax ID(s): 106590 (species)

SI-ID 215451

DSM 13439

Other strains from Cupriavidus necator

Section

Cupriavidus necator H850-4LT is a mesophilic bacterium that was isolated from strain H850 genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase, xylXYZ of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway..

mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Cupriavidus*
	Species	**Cupriavidus necator**
	Full Scientific Name (LPSN)	Cupriavidus necator Makkar and Casida 1987

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5047]

Culture medium

REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)

[Ref.: #5047]

Culture medium growth

[Ref.: #5047]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5047]

Culture medium composition

expand / minimize

[Ref.: #5047]

Culture medium

MINERAL MEDIUM WITH VITAMINS (DSMZ Medium 462)

[Ref.: #5047]

Culture medium growth

[Ref.: #5047]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5047]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5047]			growth	28 °C

[Ref.: #5047]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68369]	beta-glucosidase	-	3.2.1.21	* from API 20NE
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #5047]	23373	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5047]	Sample type/isolated from	strain H850 (NRRL B-15940) genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase, xylXYZ of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.
[Ref.: #5047]	Geographic location (country and/or sea, region)	H850 was isolated from Hudson River dredge, USA
* marker position based on {}

Isolation sources categories

#Host

#Microbial

#Bacteria

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5047]

Biosafety level

Risk group (German classification)
According to TRBA 466

Availability in culture collections External links

[Ref.: #5047]

Culture collection no.

DSM 13439

[Ref.: #71679]

SI-ID 215451

References

#5047

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13439

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#68369

Automatically annotated from API 20NE .

#71679

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID215451.1 )

* These data were automatically processed and therefore are not curated

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