Strain identifier
BacDive ID: 2004
Type strain: 
Species: Cupriavidus necator
Strain Designation: H 16 PHB-4, H 16 PHB-4
Strain history: <- IMG <- H.G. Schlegel, H 16 PHB-4
NCBI tax ID(s): 106590 (species)
version 8.1 (current version)
General
@ref: 134
BacDive-ID: 2004
DSM-Number: 541
keywords: Bacteria, mesophilic
description: Cupriavidus necator H 16 PHB-4 is a mesophilic bacterium of the family Burkholderiaceae.
NCBI tax id
- NCBI tax id: 106590
- Matching level: species
strain history
- @ref: 134
- history: <- IMG <- H.G. Schlegel, H 16 PHB-4
doi: 10.13145/bacdive2004.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Cupriavidus
- species: Cupriavidus necator
- full scientific name: Cupriavidus necator Makkar and Casida 1987
@ref: 134
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Cupriavidus
species: Cupriavidus necator
full scientific name: Cupriavidus necator Makkar and Casida 1987
strain designation: H 16 PHB-4, H 16 PHB-4
type strain: no
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 134 | MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) | yes | https://mediadive.dsmz.de/medium/81 | Name: MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) (DSMZ Medium 81) Composition: Agar 20.1005 g/l Na2HPO4 x 2 H2O 2.91457 g/l KH2PO4 2.31156 g/l NH4Cl 1.00503 g/l MgSO4 x 7 H2O 0.502512 g/l Ferric ammonium citrate 0.0502513 g/l CaCl2 x 2 H2O 0.0100503 g/l NaVO3 x H2O 0.00502512 g/l Calcium pantothenate 0.00251256 g/l Pyridoxine hydrochloride 0.00251256 g/l Nicotinic acid 0.00251256 g/l Thiamine-HCl x 2 H2O 0.00251256 g/l H3BO3 0.00150754 g/l CoCl2 x 6 H2O 0.00100503 g/l Riboflavin 0.000502513 g/l ZnSO4 x 7 H2O 0.000502513 g/l MnCl2 x 4 H2O 0.000150754 g/l Na2MoO4 x 2 H2O 0.000150754 g/l NiCl2 x 6 H2O 0.000100503 g/l CuCl2 x 2 H2O 5.02513e-05 g/l Vitamin B12 5.02513e-05 g/l Folic acid 1.00503e-05 g/l Biotin 5.02513e-06 g/l Distilled water |
| 134 | NUTRIENT AGAR (DSMZ Medium 1) | yes | https://mediadive.dsmz.de/medium/1 | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water |
culture temp
- @ref: 134
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
compound production
| @ref | compound |
|---|---|
| 134 | ribonuclease |
| 134 | ribulose-1,5-bisphosphate carboxylase |
| 134 | glycollate |
| 134 | single cell protein |
Safety information
risk assessment
- @ref: 134
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
External links
@ref: 134
culture collection no.: DSM 541
straininfo link
- @ref: 71651
- straininfo: 47208
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 10803898 | Sequence of PHA synthase gene from two strains of Rhodospirillum rubrum and in vivo substrate specificity of four PHA synthases across two heterologous expression systems. | Clemente T, Shah D, Tran M, Stark D, Padgette S, Dennis D, Bruckener K, Steinbuchel A, Mitsky T | Appl Microbiol Biotechnol | 10.1007/s002530051636 | 2000 | Acyltransferases/chemistry/*genetics/*metabolism, Amino Acid Sequence, Base Sequence, Cupriavidus necator/enzymology/genetics, Genes, Bacterial, Gram-Negative Bacteria/*enzymology/genetics, Molecular Sequence Data, Pseudomonas putida/enzymology/genetics, Recombinant Proteins/metabolism, Rhodospirillum rubrum/*enzymology/genetics, Sequence Analysis, DNA, Substrate Specificity | Metabolism |
| Metabolism | 15528546 | Engineering of chimeric class II polyhydroxyalkanoate synthases. | Niamsiri N, Delamarre SC, Kim YR, Batt CA | Appl Environ Microbiol | 10.1128/AEM.70.11.6789-6799.2004 | 2004 | Acyltransferases/chemistry/*genetics/metabolism, Amino Acid Sequence, Cupriavidus necator/*enzymology/genetics, Genetic Engineering/*methods, Models, Molecular, Molecular Sequence Data, Phylogeny, Pseudomonas oleovorans/*enzymology/genetics, Recombinant Fusion Proteins/chemistry/*genetics/metabolism, Soil Microbiology | Enzymology |
| Biotechnology | 18266340 | Monitoring poly(3-hydroxybutyrate) production in cupriavidus necator DSM 428 (H16) with raman spectroscopy. | Gelder JD, Willemse-Erix D, Scholtes MJ, Sanchez JI, Maquelin K, Vandenabeele P, Boever PD, Puppels GJ, Moens L, Vos PD | Anal Chem | 10.1021/ac702185d | 2008 | ||
| Biotechnology | 32244900 | PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003. | Thomas T, Sudesh K, Bazire A, Elain A, Tan HT, Lim H, Bruzaud S | Bioengineering (Basel) | 10.3390/bioengineering7010029 | 2020 |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 134 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 541) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-541 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 71651 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID47208.1 | StrainInfo: A central database for resolving microbial strain identifiers |